scholarly journals Effect of normobaric oxygen treatment on oxidative stress and enzyme activities in guinea pig heart

2012 ◽  
Vol 31 (02) ◽  
pp. 179-184 ◽  
Author(s):  
Zuzana Tatarkova ◽  
Ivan Engler ◽  
Andrea Calkovska ◽  
Daniela Mokra ◽  
Anna Drgova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Normobaric Oxygen ◽  
Oxygen Treatment

scholarly journals Increasing Oxygen Partial Pressures Induce a Distinct Transcriptional Response in Human PBMC: A Pilot Study on the “Normobaric Oxygen Paradox”

2021 ◽  
Vol 22 (1) ◽  
pp. 458
Author(s):  
Deborah Fratantonio ◽  
Fabio Virgili ◽  
Alessandro Zucchi ◽  
Kate Lambrechts ◽  
Tiziana Latronico ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transcription Factors ◽  
Stress Response ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Oxidative Stress Response ◽  
Molecular Characteristics ◽  
Nuclear Factor ◽  
Normobaric Oxygen ◽  
Oxygen Paradox

The term “normobaric oxygen paradox” (NOP), describes the response to the return to normoxia after a hyperoxic event, sensed by tissues as oxygen shortage, and resulting in up-regulation of the Hypoxia-inducible factor 1α (HIF-1α) transcription factor activity. The molecular characteristics of this response have not been yet fully characterized. Herein, we report the activation time trend of oxygen-sensitive transcription factors in human peripheral blood mononuclear cells (PBMCs) obtained from healthy subjects after one hour of exposure to mild (MH), high (HH) and very high (VHH) hyperoxia, corresponding to 30%, 100%, 140% O2, respectively. Our observations confirm that MH is perceived as a hypoxic stress, characterized by the activation of HIF-1α and Nuclear factor (erythroid-derived 2)-like 2 (NRF2), but not Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB). Conversely, HH is associated to a progressive loss of NOP response and to an increase in oxidative stress leading to NRF2 and NF-kB activation, accompanied by the synthesis of glutathione (GSH). After VHH, HIF-1α activation is totally absent and oxidative stress response, accompanied by NF-κB activation, is prevalent. Intracellular GSH and Matrix metallopeptidase 9 (MMP-9) plasma levels parallel the transcription factors activation pattern and remain elevated throughout the observation time. In conclusion, our study confirms that, in vivo, the return to normoxia after MH is sensed as a hypoxic trigger characterized by HIF-1α activation. On the contrary, HH and VHH induce a shift toward an oxidative stress response, characterized by NRF2 and NF-κB activation in the first 24 h post exposure.

scholarly journals Hyperbaric Oxygen Treatment: Effects on Mitochondrial Function and Oxidative Stress

Biomolecules ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1827
Author(s):  
Nofar Schottlender ◽  
Irit Gottfried ◽  
Uri Ashery
Keyword(s):  
Oxidative Stress ◽  
Hyperbaric Oxygen ◽  
Mitochondrial Function ◽  
Defense Mechanisms ◽  
Term Treatment ◽  
Mitochondrial Activity ◽  
Hyperbaric Oxygen Treatment ◽  
Oxygen Treatment ◽  
Long Term Treatment ◽  
And Oxidative Stress

Hyperbaric oxygen treatment (HBOT)—the administration of 100% oxygen at atmospheric pressure (ATA) greater than 1 ATA—increases the proportion of dissolved oxygen in the blood five- to twenty-fold. This increase in accessible oxygen places the mitochondrion—the organelle that consumes most of the oxygen that we breathe—at the epicenter of HBOT’s effects. As the mitochondrion is also a major site for the production of reactive oxygen species (ROS), it is possible that HBOT will increase also oxidative stress. Depending on the conditions of the HBO treatment (duration, pressure, umber of treatments), short-term treatments have been shown to have deleterious effects on both mitochondrial activity and production of ROS. Long-term treatment, on the other hand, improves mitochondrial activity and leads to a decrease in ROS levels, partially due to the effects of HBOT, which increases antioxidant defense mechanisms. Many diseases and conditions are characterized by mitochondrial dysfunction and imbalance between ROS and antioxidant scavengers, suggesting potential therapeutic intervention for HBOT. In the present review, we will present current views on the effects of HBOT on mitochondrial function and oxidative stress, the interplay between them and the implications for several diseases.

scholarly journals The Effects of Hyperbaric Oxygen at Different Pressures on Oxidative Stress and Antioxidant Status in Rats

Medicina ◽  
2019 ◽  
Vol 55 (5) ◽  
pp. 205 ◽  
Author(s):  
Şefika Körpınar ◽  
Hafize Uzun
Keyword(s):  
Oxidative Stress ◽  
Hyperbaric Oxygen ◽  
Antioxidant Status ◽  
Central Retinal Artery Occlusion ◽  
Retinal Artery Occlusion ◽  
Antioxidant Response ◽  
Control Group ◽  
Albino Rats ◽  
Normobaric Oxygen ◽  
Group I

Background: The optimal use of oxygen at greater than atmospheric pressures in any operational or therapeutic application (hyperbaric oxygen, HBO2) requires awareness of the fact that the beneficial effects of oxygen coexist with toxic effects depending on the pressure and duration of exposure. In this study, we aimed to investigate the effect of HBO2 therapy on oxidative stress and antioxidant status in commonly used protocol for acute HBO2 indications, such as carbon monoxide intoxication, central retinal artery occlusion, crush injury, gas gangrene, and to compare it with normobaric oxygen (NBO2) in healthy rats. Materials and Methods: Fifty-six male, young adult Wistar albino rats were randomly divided into seven groups and named as Group I through Group VII. Plasma malondialdehyde (MDA), superoxide dismutase (SOD), and erythrocyte glutathione (GSH) levels in control group were compared to the levels in other groups. Results: The increases in MDA levels and the decrease in SOD activities were statistically significant in HBO2 groups at the end of the first 24 h when compared to the control group, and the significant decrease in erythrocyte GSH level was only at 2.4 atmospheres absolute. Conclusions: The present study showed that pressure and frequency of exposure are important factors to consider when investigating HBO2-induced oxidative stress and antioxidant response.

scholarly journals Advantages and Disadvantages of Hyperbaric Oxygen Treatment in Mice with Obesity Hyperlipidemia and Steatohepatitis

2011 ◽  
Vol 11 ◽  
pp. 2124-2135 ◽  
Author(s):  
Koichi Tsuneyama ◽  
Yen-Chen Chen ◽  
Makoto Fujimoto ◽  
Yoshiyuki Sasaki ◽  
Wataru Suzuki ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hyperbaric Oxygen ◽  
Lipid Level ◽  
The Body ◽  
Cellular Damage ◽  
Control Group ◽  
Second Phase ◽  
Hyperbaric Oxygen Treatment ◽  
Advantages And Disadvantages ◽  
Oxygen Treatment

The effect of hyperbaric oxygen treatment (HBOT) was examined using MSG mice, which are an animal model of obesity, hyperlipidemia, diabetes, and nonalcoholic fatty liver disease. Nineteen MSG male mice were divided into HBOT treated and control groups at 12 weeks of ages. The HBOT group was treated with hyperbaric oxygen from 12 to 14 weeks (first phase) and then from 16 to 18 weeks (second phase). Interestingly, the body weight of the HBOT group was significantly lower (P<0.01) than that of the control group. In contrast, the serum lipid level did not show significant changes between the two groups. As for the effects of increasing oxidative stress, the liver histology of the HBOT group showed severer cellular damage and aberrant TNF-α expression. HBOT has the advantage of improving obesity in patients with metabolic syndrome, but the fault of causing organ damage by increasing oxidative stress.

scholarly journals An Analysis of Patients With Carbon Monoxide Poisoning Transported by a Physician-Staffed Helicopter

2019 ◽  
pp. 43-47
Author(s):  
iroki Nagasawa ◽  
Youichi Yanagawa
Keyword(s):  
Carbon Monoxide ◽  
Study Design ◽  
Carbon Monoxide Poisoning ◽  
Male Gender ◽  
Co Poisoning ◽  
Middle Aged ◽  
Normobaric Oxygen ◽  
Base Hospital ◽  
Oxygen Treatment ◽  
Before And After

OBJECTIVE: We retrospectively investigated a series of patients with carbon monoxide (CO) poisoning transported by a physician-staffed helicopter (DH) to seek evidence supporting the daily management of patients with CO poisoning. STUDY DESIGN: Subjects were divided into two groups: the carboxyhemoglobin saturation (SpCO) (+) group, which included patients who had a pulse CO-oximeter attached during transportation; and the SpCO (-) group, which included all other patients. We compared the level of SpCO before and after transportation when the level was noted. RESULTS: The subjects were predominantly middle-aged and male. Seventeen subjects (53.1%) received a diagnosis of CO poisoning based on the situation in which the subjects were found, without the carboxyhemoglobin level being measured. No subjects show deterioration of their condition after transportation, and none ultimately died. The frequency of male gender, normobaric oxygen treatment and return to the base hospital in the SpCO (+) group was significantly greater than in the SpCO (-) group. The SpCO levels after transportation were significantly lower than they had been before transportation. CONCLUSION: This is the first study to show the results of the analysis of patients with CO poisoning transported by the DH. All patients with CO poisoning were safely transported.

Normobaric oxygen treatment improves neuronal survival functional recovery and axonal plasticity after newborn hypoxia-ischemia

2020 ◽  
Vol 379 ◽  
pp. 112338
Author(s):  
Taha Kelestemur ◽  
Mustafa C. Beker ◽  
Ahmet B. Caglayan ◽  
Berrak Caglayan ◽  
Serdar Altunay ◽  
...  
Keyword(s):  
Functional Recovery ◽  
Neuronal Survival ◽  
Normobaric Oxygen ◽  
Axonal Plasticity ◽  
Oxygen Treatment ◽  
Hypoxia Ischemia

scholarly journals Early and late effects of hyperbaric oxygen treatment on oxidative stress parameters in diabetic patients

2008 ◽  
pp. 41-47
Author(s):  
F Gürdöl ◽  
M Cimşit ◽  
YZ Öner-İyidoğan ◽  
Ş Körpinar ◽  
S Yalçinkaya ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hyperbaric Oxygen ◽  
Biological Tissues ◽  
Foot Ulcers ◽  
Diabetic Patients ◽  
Hyperbaric Oxygen Treatment ◽  
Endogenous Factors ◽  
Oxidative Stress Parameters ◽  
Oxygen Treatment ◽  
Stress Parameters

Exposure to hyperbaric oxygen leads to increased amount of reactive oxygen species (ROS) that are derived from various sources. After the discovery that ROS can function as signaling molecules, the idea of ROS being hazardous to biological tissues has been challenged. The aim of this study was to examine the changes in oxidative stress parameters in diabetics undergoing hyperbaric oxygen therapy (HBOT) due to foot ulcers. Twenty patients, who received HBOT for diabetic foot ulcers, were included in the study. Blood samples were taken before HBOT and 30 min after exit from the chamber, on the day of the first and the 15th HBOT sessions. They were used for the determinations of malondialdehyde (MDA), 8-isoprostane and advanced oxidation protein products (AOPPs). 8-Isoprostane and AOPP levels were not altered significantly after the first HBOT session, while both were increased on the fifteenth day (p<0.05). MDA was significantly increased only after the first HBOT session, and remained unchanged on the fifteenth day (within-day variations). Plasma AOPP levels were lowered significantly after fifteen consecutive HBOT sessions (between-day variations). Decreased AOPP levels suggest that increased oxygenation of tissues due to HBO therapy may activate some endogenous factors that prevent hazardous effects of the disease itself.

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