scholarly journals Quantification of Pharmacologically Active Marker Gallic Acid and Ellagic Acid from Leaf and Stem of Pergularia daemia Forsk. by HPTLC Method

2016 ◽  
Vol 7 (1) ◽  
Keyword(s):  
Gallic Acid ◽  
Ellagic Acid ◽  
Pharmacologically Active ◽  
Hptlc Method

HPTLC Method for Estimation of Ellagic Acid and Gallic Acid from Flowers of Woodfordia fruticosa Kurz.

2021 ◽  
Vol 11 (3) ◽  
pp. 187-192
Author(s):  
Bhaskar O. Aher ◽  
Yogesh T. Sonawane ◽  
Vinod A. Bairagi ◽  
Parag A. Pathade
Keyword(s):  
Gallic Acid ◽  
Ellagic Acid ◽  
Total Content ◽  
Medicinal Use ◽  
Wide Range ◽  
Bowel Complaint ◽  
History Of ◽  
Marker Compounds ◽  
Hptlc Method ◽  
Woodfordia Fruticosa

Woodfordia fruticosa Kurz, belonging to family Lythraceae, commonly known as dhataki pushpa, is a plant of tropical and subtropical region with a long history of medicinal use. Wide range of chemical compounds including tannins, flavonoides, anthraquinones glycosides and polyphenols have been isolated from the plant. The extract from the flowers are used in folklore medicine for treatments of like wound healing, bowel complaint, rheumatism, hematuria. Litreture survey indicates that the flowers are rich in tannins, both gallo and ellagitannins. Hence, it was thought worthwhile to use Ellagic acid and Gallic acid as the marker compounds for the standardization of the flowers. With this background the present study was undertaken to standardize the flowers using Ellagic acid and Gallic acid as marker compounds. The HPTLC method used for the standardization was validated for the parameters like specificity, limits of detection and quantification, linearity, precision, accuracy and recovery. The total content of Ellagic acid and Gallic acid in the Methanolic extract was found to be 4.21%, 6.70% and the total content of Ellagic acid and Gallic acid in the Total aqueous extract was found to be 2.62%, 4.75%.

scholarly journals Comparative HPTLC Estimation and Antibactrial Effect of Ellagic Acid, Gallic Acid and Ethanolic Extract of Syzygium cumini Seeds Under Accelerated Storage Condition

2018 ◽  
Vol 9 (07) ◽  
Author(s):  
Dalavi N. B. ◽  
Gawali V. B ◽  
Bhalsing M. D.
Keyword(s):  
Antibacterial Activity ◽  
Gallic Acid ◽  
Ellagic Acid ◽  
Bacterial Strain ◽  
Ethanolic Extract ◽  
High Sensitivity ◽  
Storage Condition ◽  
Syzygium Cumini ◽  
Accelerated Storage ◽  
Hptlc Method

Syzygium cumini seed (L.) Skeels (myrtaceae) commonly known as “jamun” is widely used in Ayurveda. The main active constituents present in syzygium cumini seed is Ellagic and Ellagic acid. Syzygium cumini seed is official in Indian Ayurvedic pharmacopia. The comparative study of assay by HPTLC method and antibacterial activity was done for the Ellagic and Gallic acid and S.cumini extract at accelerated storage condition for a period of 6 months.The antibacterial activity of Ellagic acid, Gallic acid and ethanolic extract of dried seeds of s.cumini was determined by cup plate technique against gram-positive bacterial strain (Staphylococcus aureus) and gram-negative bacterial strain (Escherichia coli). For HPTLC method Ellagic acid, Gallic acid and extract was spotted on the plates precoated with silica gel 60 F254 and developed using toluene: ethyl acetate: formic acid, (6:6:1.5v/v/v) as mobile phase. Densitometry analysis was carried out at 271 nm. The method showed high sensitivity with good linearity over the concentration range of 200-1000ng/spot. The peak for Ellagic acid and Gallic acid were observed at Rf of 0.47 ±0.02 and 0.57±0.02 resp. The aim of our study was to observe the effect of accelerated storage on markers and extract. The analysis was carried out at 1,2,3,6 months study as per ICH guidelines for stability testing of drug at storage condition of 40ºC ± 2ºC/75% RH ± 5% RH. A decrease in antibacterial potential of the extract was observed with the simultaneous reduction in the % assay after a 6 month study. This method can be used for the quality control of the extract as well as markers.

Phytochemical Investigation and Antimutagenic Potential of Ethanolic Extracts of Emblica officinalis, Terminalia chebula and Terminalia bellirica

2020 ◽  
Vol 10 (4) ◽  
pp. 488-494 ◽  
Author(s):  
Venugopal Singamaneni ◽  
Sudheer Kumar Dokuparthi ◽  
Nilanjana Banerjee ◽  
Ashish Kumar ◽  
Tulika Chakrabarti
Keyword(s):  
Ethyl Acetate ◽  
Gallic Acid ◽  
Ellagic Acid ◽  
Antimutagenic Activity ◽  
Dependent Manner ◽  
Terminalia Chebula ◽  
Emblica Officinalis ◽  
Dried Fruits ◽  
Terminalia Bellerica ◽  
The Family

Background: Emblica officinalis Gaertn. which belongs to the family Euphorbiaceae, Terminalia chebula Retz. and Terminalia bellerica Roxb. belong to the family Combretaceae. These are well known medicinal plants with phytochemical reservoir of great medicinal values and possess a vast ethnomedical history. Objective: The aim of the present study is to isolation of major compounds and to evaluate antimutagenic potential of the ethanol extracts of these plants. Methods: The dried fruits of E. officinalis, T. bellirica and T. chebula were powdered and extracted with 95% ethanol. The ethyl acetate portions were chromatographed over silica gel to isolate major compounds. Antimutagenic activity was determined by Ames test using TA98 and TA100 strains of Salmonella typhimurium. Results: Two major known compounds, gallic acid and ellagic acid were isolated from the dried fruits of Emblica officinalis, Terminalia chebula and T. bellirica. All the three extracts counteracted the mutagenicity induced by different genotoxic compounds in a dose dependent manner. Conclusion: This study showed that ethyl acetate portion of three extracts contain two major compounds, gallic acid and ellagic acid which might be responsible for potent antimutagenic activity of these extracts.

Study on Active Ingredient of Blueberry Pomace in Fermentation by Lentinus edodes Hypha

2014 ◽  
Vol 926-930 ◽  
pp. 238-241
Author(s):  
Li Guo ◽  
Peng Wang
Keyword(s):  
Gallic Acid ◽  
Active Ingredient ◽  
Ellagic Acid ◽  
Acid Content ◽  
Hplc Method ◽  
Spectrophotometric Analysis ◽  
Anthocyanin Content ◽  
Lentinus Edodes ◽  
Flavonoid Content ◽  
Fermentation Products

The effect of fermentation by Lentinus edodes hypha on active ingredient of blueberry pomace was studied, and the changes of fermentation products with protein, flavonoid and anthocyanin content were evaluated by spectrophotometric analysis. The protein content decreased slowly, and the flavonoid content in the first 66 h increased the highest level 0.09 mg/g, the anthocyanin content decreased during the first fermentation process, then increased to 0.568 mg/g at 30 h. The fermentation product of ellagic acid and gallic acid content were analyzed by using HPLC Method, the ellagic acid content decreased, and the gallic acid content increased first and then decreased, reached the highest value for the 0.310 mg/g in the first 54 h.

DEVELOPMENT AND VALIDATION OF A CHEMOMETRICS ASSISTED SPECTROSCOPIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF GALLIC ACID, ELLAGIC ACID AND CURCUMIN IN POLYHERBAL ANTIDIABETIC FORMULATIONS

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (06) ◽  
pp. 67-73
Author(s):  
M. A Shah ◽  
◽  
H. U. Patel ◽  
H.A Raj
Keyword(s):  
Gallic Acid ◽  
Ellagic Acid ◽  
Spectroscopic Method ◽  
Least Square ◽  
Data Matrix ◽  
Simultaneous Estimation ◽  
Chemometric Methods ◽  
Mixed Solutions ◽  
Validation Set ◽  
Development And Validation

Two chemometric methods, Inverse Least Square (ILS) and Classical Least Square (CLS), were applied for the simultaneous estimation of gallic acid, ellagic acid and curcumin in polyherbal antidiabetic formulation. Twenty mixed solutions were prepared for the chemometric calibration as training set and 10 mixed solutions were prepared as validation set. The absorbance data matrix was obtained by measuring the absorbance at 20 different wavelengths, from 241 to 279 nm with the interval of 2 nm (Δλ= 2 nm). The developed calibrations were successfully tested for three antidiabetic polyherbal formulations for their gallic acid, ellagic acid and curcumin contents. Developed methods were validated and root mean square error of precision (RMSEP) was determined. Both chemometric methods in this study can be satisfactorily used for the quantitative analysis in polyherbal dosage forms. The chemometric calculations were performed by using the chemometrics toolbox with MATLAB R2015a software.

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