HPTLC Method for Estimation of Ellagic Acid and Gallic Acid from Flowers of Woodfordia fruticosa Kurz.

2021 ◽  
Vol 11 (3) ◽  
pp. 187-192
Author(s):  
Bhaskar O. Aher ◽  
Yogesh T. Sonawane ◽  
Vinod A. Bairagi ◽  
Parag A. Pathade
Keyword(s):  
Gallic Acid ◽  
Ellagic Acid ◽  
Total Content ◽  
Medicinal Use ◽  
Wide Range ◽  
Bowel Complaint ◽  
History Of ◽  
Marker Compounds ◽  
Hptlc Method ◽  
Woodfordia Fruticosa

Woodfordia fruticosa Kurz, belonging to family Lythraceae, commonly known as dhataki pushpa, is a plant of tropical and subtropical region with a long history of medicinal use. Wide range of chemical compounds including tannins, flavonoides, anthraquinones glycosides and polyphenols have been isolated from the plant. The extract from the flowers are used in folklore medicine for treatments of like wound healing, bowel complaint, rheumatism, hematuria. Litreture survey indicates that the flowers are rich in tannins, both gallo and ellagitannins. Hence, it was thought worthwhile to use Ellagic acid and Gallic acid as the marker compounds for the standardization of the flowers. With this background the present study was undertaken to standardize the flowers using Ellagic acid and Gallic acid as marker compounds. The HPTLC method used for the standardization was validated for the parameters like specificity, limits of detection and quantification, linearity, precision, accuracy and recovery. The total content of Ellagic acid and Gallic acid in the Methanolic extract was found to be 4.21%, 6.70% and the total content of Ellagic acid and Gallic acid in the Total aqueous extract was found to be 2.62%, 4.75%.

scholarly journals A Review on Woodfordia fruticosa Kurz (Dhatki): Ayurvedic, Folk and Modern Uses

2021 ◽  
Vol 11 (3) ◽  
pp. 126-131
Author(s):  
Shifali Thakur ◽  
Hemlata Kaurav ◽  
Gitika Chaudhary
Keyword(s):  
Biological Activities ◽  
Chemical Constituents ◽  
Anthraquinone Glycosides ◽  
Medicinal Use ◽  
The Family ◽  
History Of ◽  
Phytochemical Compounds ◽  
Therapeutic Activities ◽  
Herbal Plant ◽  
Woodfordia Fruticosa

Woodfordia fruticosa kurz is an herbal plant that belongs to the family Lythraceae. This plant is widely distributed throughout the tropical and subtropical regions of India, Sri Lanka, China, Malaysia, Indonesia, Japan and Pakistan. The plant possesses a long history of medicinal use. The flowers of Woodfordia fruticosa are recorded to possess potent therapeutic values. The various phytochemicals isolated from this plant are tannins, flavonoids, anthraquinone, glycosides and polyphenols. The extract of flowers and leaves are associated with useful therapeutic activities. These phytochemical compounds have many pharmacological properties such as antimicrobial, hepatoprotective, cardioprotective, antioxidant, antiulcer, immunomodulatory, antifertility and anti-tumor.  These pharmacological activities of Woodfordia fruticosa plant are also mentioned in the literature of ayurveda, yunani and other traditional systems of medicine. This review is intended to provide brief information on the plant Woodfordia fruticosa on the basis of chemical constituents, folk usage, ayurvedic usage, modern usage and its biological activities.  Keywords: Woodfordia fruticosa, antimicrobial, Ayurvedic, flavonoids, Octacosanol 

scholarly journals Identification and quantification of phenolic compounds in pomegranate juices from eight Macedonian cultivars

2019 ◽  
Vol 38 (2) ◽  
pp. 149
Author(s):  
Jasmina Petreska Stanoeva ◽  
Nina Peneva ◽  
Marina Stefova ◽  
Viktor Gjamovski
Keyword(s):  
Chemical Composition ◽  
Phenolic Compounds ◽  
Ellagic Acid ◽  
Total Content ◽  
Punica Granatum ◽  
Wide Range ◽  
Health Promoting ◽  
Punica Granatum L ◽  
Unique Chemical

Punica granatum L. is one of the species enjoying growing interest due to its complex and unique chemical composition that encompasses the presence of anthocyanins, ellagic acid and ellagitannins, gallic acid and gallotannins, proanthocyanidins, flavanols and lignans. This combination is deemed responsible for a wide range of health-promoting biological activities.This study was focused on the analysis of flavonoids, anthocyanins and phenolic acids in eight pomegranate varieties (Punica granatum) from Macedonia, in two consecutive years. Fruits from each cultivar were washed and manually peeled, and the juice was filtered. NaF (8.5 mg) was added to 100 ml juice as a stabilizer. The samples were centrifuged for 15 min at 3000 rpm and analyzed using an HPLC/DAD/MSn method that was optimized for determination of their polyphenolic fingerprints.The dominant anthocyanin in all pomegranate varieties was cyanidin-3-glucoside followed by cyanidin and delphinidin 3,5-diglucoside. From the results, it can be concluded that the content of anthocyanins was higher in 2016 compared to 2017. But in contrast, the total content of non-colored polyphenols was around 10 times lower in 2016 compared to the amount found in the same samples in 2017.

scholarly journals SIMULTANEOUS ESTIMATION OF CURCUMINOIDS, PIPERINE, AND GALLIC ACID IN AN AYURVEDIC FORMULATION BY VALIDATED HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHIC METHOD

2016 ◽  
pp. 117
Author(s):  
Niraj Yashvantrai Vyas ◽  
Sejal Patel
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
High Performance ◽  
Retention Factor ◽  
Simultaneous Estimation ◽  
Phase Plate ◽  
Analytical Work ◽  
Ambient Room Temperature ◽  
Marker Compounds ◽  
Hptlc Method

<p>ABSTRACT<br />Objective: The present study was proposed to quantitatively estimate the amount of three marker compounds; curcuminoids, piperine, and gallic<br />acid in a multicomponent ayurvedic formulation using high-performance thin layer chromatographic (HPTLC) method for routine analytical work.<br />Methods: TLC separation was performed on silica gel 60 F<br /> plates using toluene:ethyl acetate:formic acid:methanol (5.6:2.2:1.2:1.0 v/v/v/v) as<br />mobile phase. Plate was developed by to a distance of 90 mm at ambient room temperature with 20 minutes saturation time. Densitometric analysis<br />was performed at 327 nm. Method was validated as per International Conference on Harmonization Q2 (R1) guideline also.<br />254<br />Results: Piperine, curcuminoids, and gallic acid were separated on TLC at retention factor values of 0.71, 0.61, and 0.29, respectively. The described<br />method was linear over the range of 300-700 ng/spot, 100-300 ng/spot, and 250-550 ng/spot, respectively, for curcuminoids, piperine, and gallic<br />acid. The accuracy of the method was assessed by recovery studies and was found to be 101.71%, 99.67%, and 99.59% for curcuminoids, piperine,<br />and gallic acid, respectively. The amount of curcuminoids, piperine, and gallic acid in the ayurvedic formulation was found to be 3.99% w/w, 1.9%<br />w/w, and 0.8% w/w, respectively, when analyzed quantitatively by developed validated HPTLC method.<br />Conclusion: The method can be used as a tool for quality control of herbal formulation.<br />Keywords: Curcuminoids, Piperine, Gallic acid, High-performance thin layer chromatographic.</p>

scholarly journals Comparative HPTLC Estimation and Antibactrial Effect of Ellagic Acid, Gallic Acid and Ethanolic Extract of Syzygium cumini Seeds Under Accelerated Storage Condition

2018 ◽  
Vol 9 (07) ◽  
Author(s):  
Dalavi N. B. ◽  
Gawali V. B ◽  
Bhalsing M. D.
Keyword(s):  
Antibacterial Activity ◽  
Gallic Acid ◽  
Ellagic Acid ◽  
Bacterial Strain ◽  
Ethanolic Extract ◽  
High Sensitivity ◽  
Storage Condition ◽  
Syzygium Cumini ◽  
Accelerated Storage ◽  
Hptlc Method

Syzygium cumini seed (L.) Skeels (myrtaceae) commonly known as “jamun” is widely used in Ayurveda. The main active constituents present in syzygium cumini seed is Ellagic and Ellagic acid. Syzygium cumini seed is official in Indian Ayurvedic pharmacopia. The comparative study of assay by HPTLC method and antibacterial activity was done for the Ellagic and Gallic acid and S.cumini extract at accelerated storage condition for a period of 6 months.The antibacterial activity of Ellagic acid, Gallic acid and ethanolic extract of dried seeds of s.cumini was determined by cup plate technique against gram-positive bacterial strain (Staphylococcus aureus) and gram-negative bacterial strain (Escherichia coli). For HPTLC method Ellagic acid, Gallic acid and extract was spotted on the plates precoated with silica gel 60 F254 and developed using toluene: ethyl acetate: formic acid, (6:6:1.5v/v/v) as mobile phase. Densitometry analysis was carried out at 271 nm. The method showed high sensitivity with good linearity over the concentration range of 200-1000ng/spot. The peak for Ellagic acid and Gallic acid were observed at Rf of 0.47 ±0.02 and 0.57±0.02 resp. The aim of our study was to observe the effect of accelerated storage on markers and extract. The analysis was carried out at 1,2,3,6 months study as per ICH guidelines for stability testing of drug at storage condition of 40ºC ± 2ºC/75% RH ± 5% RH. A decrease in antibacterial potential of the extract was observed with the simultaneous reduction in the % assay after a 6 month study. This method can be used for the quality control of the extract as well as markers.

Group Experiences and Individual Differences in Stuttering

2019 ◽  
Vol 62 (12) ◽  
pp. 4335-4350 ◽  
Author(s):  
Seth E. Tichenor ◽  
J. Scott Yaruss
Keyword(s):  
Individual Differences ◽  
Support Groups ◽  
Support Group ◽  
Affective States ◽  
Life Outcomes ◽  
Self Help ◽  
Wide Range ◽  
Group Experiences ◽  
History Of ◽  
Adults Who Stutter

Purpose This study explored group experiences and individual differences in the behaviors, thoughts, and feelings perceived by adults who stutter. Respondents' goals when speaking and prior participation in self-help/support groups were used to predict individual differences in reported behaviors, thoughts, and feelings. Method In this study, 502 adults who stutter completed a survey examining their behaviors, thoughts, and feelings in and around moments of stuttering. Data were analyzed to determine distributions of group and individual experiences. Results Speakers reported experiencing a wide range of both overt behaviors (e.g., repetitions) and covert behaviors (e.g., remaining silent, choosing not to speak). Having the goal of not stuttering when speaking was significantly associated with more covert behaviors and more negative cognitive and affective states, whereas a history of self-help/support group participation was significantly associated with a decreased probability of these behaviors and states. Conclusion Data from this survey suggest that participating in self-help/support groups and having a goal of communicating freely (as opposed to trying not to stutter) are associated with less negative life outcomes due to stuttering. Results further indicate that the behaviors, thoughts, and experiences most commonly reported by speakers may not be those that are most readily observed by listeners.

Introduction: Emotion, History and the Arts

2018 ◽  
Vol 7 (2) ◽  
pp. 117-128 ◽  
Author(s):  
Erin Sullivan ◽  
Marie Louise Herzfeld-Schild
Keyword(s):  
Historical Change ◽  
Carson Mccullers ◽  
Emotional Life ◽  
Review Section ◽  
The Arts ◽  
Wide Range ◽  
War Correspondents ◽  
History Of ◽  
Johan Huizinga

This introduction surveys the rise of the history of emotions as a field and the role of the arts in such developments. Reflecting on the foundational role of the arts in the early emotion-oriented histories of Johan Huizinga and Jacob Burkhardt, as well as the concerns about methodological impressionism that have sometimes arisen in response to such studies, the introduction considers how intensive engagements with the arts can open up new insights into past emotions while still being historically and theoretically rigorous. Drawing on a wide range of emotionally charged art works from different times and places—including the novels of Carson McCullers and Harriet Beecher-Stowe, the private poetry of neo-Confucian Chinese civil servants, the photojournalism of twentieth-century war correspondents, and music from Igor Stravinsky to the Beatles—the introduction proposes five ways in which art in all its forms contributes to emotional life and consequently to emotional histories: first, by incubating deep emotional experiences that contribute to formations of identity; second, by acting as a place for the expression of private or deviant emotions; third, by functioning as a barometer of wider cultural and attitudinal change; fourth, by serving as an engine of momentous historical change; and fifth, by working as a tool for emotional connection across communities, both within specific time periods but also across them. The introduction finishes by outlining how the special issue's five articles and review section address each of these categories, while also illustrating new methodological possibilities for the field.

History of the Study of Apocrypha in Early Medieval England

2020 ◽  
Vol 48 (3-4) ◽  
pp. 13-26
Author(s):  
Brandon W. Hawk
Keyword(s):  
Old Testament ◽  
Medieval England ◽  
Medieval Period ◽  
Early Medieval ◽  
Future Directions ◽  
Early Judaism ◽  
Wide Range ◽  
History Of ◽  
The Subject ◽  
Early English

Literature written in England between about 500 and 1100 CE attests to a wide range of traditions, although it is clear that Christian sources were the most influential. Biblical apocrypha feature prominently across this corpus of literature, as early English authors clearly relied on a range of extra-biblical texts and traditions related to works under the umbrella of what have been called “Old Testament Pseudepigrapha” and “New Testament/Christian Apocrypha." While scholars of pseudepigrapha and apocrypha have long trained their eyes upon literature from the first few centuries of early Judaism and early Christianity, the medieval period has much to offer. This article presents a survey of significant developments and key threads in the history of scholarship on apocrypha in early medieval England. My purpose is not to offer a comprehensive bibliography, but to highlight major studies that have focused on the transmission of specific apocrypha, contributed to knowledge about medieval uses of apocrypha, and shaped the field from the nineteenth century up to the present. Bringing together major publications on the subject presents a striking picture of the state of the field as well as future directions.

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