scholarly journals An overview of the enzyme: Amylase and its industrial potentials

2020 ◽  
Vol 12 (1) ◽  
pp. 352-358
Author(s):  
M.A. Ahmad ◽  
U. Isah ◽  
I.A. Raubilu ◽  
S.I. Muhammad ◽  
D. Ibrahim
Keyword(s):  
Capital Investment ◽  
Simple Technique ◽  
Energy Requirement ◽  
Industrial Applications ◽  
High Yield ◽  
Physical Parameters ◽  
Scale Production ◽  
Pharmaceutical Industries ◽  
As Effects ◽  
Industrial Scale Production

Amylases are group of enzymes produced by plants, animals and microorganisms, the enzyme has the ability to hydrolyze or degrade starch molecules into polymers containing units of glucose, thus, it is one of the most useful enzymes used by industries dependent on starch in their production processes. The enzyme has varying applications in food, fermentation, textile, pharmaceutical industries among others. Generally, amylase from microbial sources (i.e. fungal and bacterial origin) has over shadowed others in industrial usage. As such, this Paper aimed at reviewing amylase enzyme as a whole and some of its common industrial applications. The review visited the types of amylase based on hydrolases classification, its sources with emphasis to microorganisms, methods of production as well as effects of some chemical and physical parameters. The review also discusses some of the most common industrial application or uses of amylase enzyme in food, brewing, chemicals, paper, pharmaceutical, textile industries to mention but few. In conclusion, the reviewers suggest the use of microbial amylase due to it easy and simple technique in production, lower capital investment, lower energy requirement and high yield during production, exploration of more microbes with enzyme production potentials as well as improved industrial Scale production of the amylase for the betterment of the economy and improved industrial production of products. Key: Amylase, Application, Enzyme, Industry, Microbes and Starch.

scholarly journals Efficient media for high production of microbial lipase from Bacillus subtilis (BSK-L) using response surface methodology for enantiopure synthesis of drug molecules

2020 ◽  
Author(s):  
Indu Bhushan
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Industrial Applications ◽  
Bioactive Molecules ◽  
High Yield ◽  
Racemic Mixture ◽  
Potential Candidate ◽  
Chiral Drugs ◽  
Microbial Lipase ◽  
Pharmaceutical Industries

Lipases are a multipurpose enzyme that holds a significant position in industrial applications due to its ability to catalyse a large number of reactions such as hydrolysis, esterification, interesterification, transesterification which makes it a potential candidate. It is also used for the separation of chiral drugs from the racemic mixture and this property of lipase is considered very important in pharmaceutical industries for the synthesis of enantiopure bioactive molecules. Assuming the tremendous importance of lipases, as stereoselective biocatalysts, in pharmaceuticals and various other commercial applications, industrial enzymologists have been forced to search for those microorganisms which are able to produce novel biocatalysts at reasonably high yield. In the present study microbial lipase was isolated from the water sample of pond at Katra, Jammu and Kashmir (India). This enzyme has shown wide specificity and higher enantioselectivity, which make it pharmaceutical important enzyme. To make it economical for industrial application, it was produced on cheap nutrient media using Response Surface Methodology and got maximum production. It was used for resolution of chiral drugs and the significant results obtained during the course of work shall have potential towards pharmaceutical industries.

scholarly journals Metabolic engineering for high yield synthesis of astaxanthin in Xanthophyllomyces dendrorhous

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Alejandro Torres-Haro ◽  
Jorge Verdín ◽  
Manuel R. Kirchmayr ◽  
Melchor Arellano-Plaza
Keyword(s):  
Metabolic Engineering ◽  
Large Scale ◽  
Dry Mass ◽  
High Yield ◽  
Xanthophyllomyces Dendrorhous ◽  
Scale Production ◽  
Proteomic Data ◽  
Large Scale Production ◽  
Mutant Strains ◽  
Pharmaceutical Industries

AbstractAstaxanthin is a carotenoid with a number of assets useful for the food, cosmetic and pharmaceutical industries. Nowadays, it is mainly produced by chemical synthesis. However, the process leads to an enantiomeric mixture where the biologically assimilable forms (3R, 3′R or 3S, 3′S) are a minority. Microbial production of (3R, 3′R) astaxanthin by Xanthophyllomyces dendrorhous is an appealing alternative due to its fast growth rate and easy large-scale production. In order to increase X. dendrorhous astaxanthin yields, random mutant strains able to produce from 6 to 10 mg/g dry mass have been generated; nevertheless, they often are unstable. On the other hand, site-directed mutant strains have also been obtained, but they increase only the yield of non-astaxanthin carotenoids. In this review, we insightfully analyze the metabolic carbon flow converging in astaxanthin biosynthesis and, by integrating the biological features of X. dendrorhous with available metabolic, genomic, transcriptomic, and proteomic data, as well as the knowledge gained with random and site-directed mutants that lead to increased carotenoids yield, we propose new metabolic engineering targets to increase astaxanthin biosynthesis.

scholarly journals Promising Immobilization of Industrial-Class Phospholipase A1 to Attain High-Yield Phospholipids Hydrolysis and Repeated Use with Optimal Water Content in Water-in-Oil Microemulsion Phase

2021 ◽  
Vol 11 (4) ◽  
pp. 1456
Author(s):  
Yusuke Hayakawa ◽  
Ryoichi Nakayama ◽  
Norikazu Namiki ◽  
Masanao Imai
Keyword(s):  
Water Content ◽  
Reaction Rate ◽  
Enzyme Reaction ◽  
Initial Reaction Rate ◽  
Industrial Applications ◽  
Molar Ratio ◽  
High Yield ◽  
Initial Reaction ◽  
Phospholipase A1 ◽  
Repeated Use

In this study, we maximized the reactivity of phospholipids hydrolysis with immobilized industrial-class phospholipase A1 (PLA1) at the desired water content in the water-in-oil (W/O) microemulsion phase. The optimal hydrophobic-hydrophilic condition of the reaction media in a hydrophobic enzyme reaction is critical to realize the maximum yields of enzyme activity of phospholipase A1. It was attributed to enzymes disliking hydrophobic surroundings as a special molecular structure for reactivity. Immobilization of PLA1 was successfully achieved with the aid of a hydrophobic carrier (Accurel MP100) combination with the treatment using glutaraldehyde. The immobilized yield was over 90% based on simple adsorption. The hydrolysis reaction was kinetically investigated through the effect of glutaraldehyde treatment of carrier and water content in the W/O microemulsion phase. The initial reaction rate increased linearly with an increasing glutaraldehyde concentration and then leveled off over a 6% glutaraldehyde concentration. The initial reaction rate, which was predominantly driven by the water content in the organic phase, changed according to a typical bell-shaped curve with respect to the molar ratio of water to phospholipid. It behaved in a similar way with different glutaraldehyde concentrations. After 10 cycles of repeated use, the reactivity was well sustained at 40% of the initial reaction rate and the creation of the final product. Accumulated yield after 10 times repetition was sufficient for industrial applications. Immobilized PLA1 has demonstrated potential as a biocatalyst for the production of phospholipid biochemicals.

Semi-industrial Scale Production of a New Yeast with Probiotic Traits, Cryptococcus sp. YMHS, Isolated from the Red Sea

2017 ◽  
Vol 10 (1) ◽  
pp. 77-88 ◽  
Author(s):  
Ashraf F. El-Baz ◽  
Hesham A. El-Enshasy ◽  
Yousseria M. Shetaia ◽  
Hoda Mahrous ◽  
Nor Zalina Othman ◽  
...  
Keyword(s):  
Red Sea ◽  
Industrial Scale ◽  
Scale Production ◽  
New Yeast ◽  
Industrial Scale Production

Isolasi dan Potensi Enzim Hidrolase Bakteri Simbion Padina sp. dari Pantai Lengkuas Belitung

2021 ◽  
Vol 23 (1) ◽  
pp. 11-17
Author(s):  
Siti Nur Jannah ◽  
Yumna Rahmadias Hanifa ◽  
Adi Budi Utomo ◽  
Ashar Kurnia Dian Prambodo ◽  
Arina Tri Lunggani
Keyword(s):  
Marine Organism ◽  
Marine Ecosystem ◽  
Gram Negative Bacteria ◽  
Scale Production ◽  
Market Demand ◽  
Bacterial Isolates ◽  
Clear Zone ◽  
Plate Method ◽  
Sea Use ◽  
Industrial Scale Production

Marine organism is one of the riches in the ocean of Indonesia. The benefits of sea use for new products produced are widely used and have high market demand. Enzymes that have marine interests have unique properties and have good benefits for industry. This study aims to isolate the bacteria that have symbionts with Padina sp and determine the potential of the enzyme hydrolase produced by these bacteria. Isolation is done by the spread plate method. Pure isolates obtained were then tested for the potential of the enzyme hydrolase on selective media. Clear zone measurements are performed to determine which bacterial isolates are good for enzyme production. The results obtained by 6 isolates of pure bacteria, all of which include Gram negative bacteria that form bacilli. All isolates had the ability to produce different Protease, Lipase, Amylase and Cellulase enzymes. The enzymes obtained from these symbiotic bacteria are expected to be used for industrial-scale production in Indonesia. In addition, the presence of this symbiont bacteria is able to reduce the level of exploitation of Padina sp and contribute to preserving the marine ecosystem.

scholarly journals Bioreactor Operating Strategies for Improved Polyhydroxyalkanoate (PHA) Productivity

Polymers ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1197 ◽  
Author(s):  
Warren Blunt ◽  
David Levin ◽  
Nazim Cicek
Keyword(s):  
Production Costs ◽  
Scale Production ◽  
Fed Batch ◽  
Continuous Bioreactor ◽  
Carbon Substrates ◽  
Pure Cultures ◽  
High Production ◽  
Operating Strategies ◽  
Major Factors ◽  
Industrial Scale Production

Microbial polyhydroxyalkanoates (PHAs) are promising biodegradable polymers that may alleviate some of the environmental burden of petroleum-derived polymers. The requirements for carbon substrates and energy for bioreactor operations are major factors contributing to the high production costs and environmental impact of PHAs. Improving the process productivity is an important aspect of cost reduction, which has been attempted using a variety of fed-batch, continuous, and semi-continuous bioreactor systems, with variable results. The purpose of this review is to summarize the bioreactor operations targeting high PHA productivity using pure cultures. The highest volumetric PHA productivity was reported more than 20 years ago for poly(3-hydroxybutryate) (PHB) production from sucrose (5.1 g L−1 h−1). In the time since, similar results have not been achieved on a scale of more than 100 L. More recently, a number fed-batch and semi-continuous (cyclic) bioreactor operation strategies have reported reasonably high productivities (1 g L−1 h−1 to 2 g L−1 h−1) under more realistic conditions for pilot or industrial-scale production, including the utilization of lower-cost waste carbon substrates and atmospheric air as the aeration medium, as well as cultivation under non-sterile conditions. Little development has occurred in the area of fully continuously fed bioreactor systems over the last eight years.

Industrial Scale Production of L-B Layers

1989 ◽  
pp. 41-49 ◽  
Author(s):  
O. Albrecht ◽  
T. Ginnai ◽  
A. Harrington ◽  
D. Marr-Leisy ◽  
V. Rodov
Keyword(s):  
Industrial Scale ◽  
Scale Production ◽  
Industrial Scale Production

scholarly journals Following the Thread: Elite Iconography on Weaving Objects at Poggio Civitate (Murlo)

Arts ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 15
Author(s):  
Nora K. Donoghue
Keyword(s):  
Large Scale ◽  
Sixth Century ◽  
Industrial Scale ◽  
Manufacturing Processes ◽  
Scale Production ◽  
Industrial Scale Production

Evidence for industrial scale production of numerous manufacturing processes has been attested in all phases of occupation at Poggio Civitate (Murlo). A subset of these, tools for the production of textiles and fibers, indicates that textile crafts were manufactured on a large scale as a part of a centralized and organized industry. These industrialized practices occurred within and around the monumental seventh and sixth century BCE complexes which displayed architectural decoration bearing iconographic themes that served to secure the positions of the aristocratic elites. This paper investigates the stamped decoration present on rocchetti and its relationship to the architectural decoration present on the monumental structures of the site. As small moveable objects used by members of the community, rocchetti present an opportunity to investigate the movement of elite images through the non-elite population of a community and their reception of aristocratic ideology presented in monumental structures.

scholarly journals Large-Scale Production of Human iPSC-Derived Macrophages for Drug Screening

2020 ◽  
Vol 21 (13) ◽  
pp. 4808 ◽  
Author(s):  
Simon Gutbier ◽  
Florian Wanke ◽  
Nadine Dahm ◽  
Anna Rümmelin ◽  
Silke Zimmermann ◽  
...  
Keyword(s):  
Drug Screening ◽  
Large Scale ◽  
Neoplastic Cell ◽  
Leukemic Cells ◽  
High Yield ◽  
Scale Production ◽  
Large Scale Production ◽  
Compound Screening ◽  
Health And Disease ◽  
Induced Pluripotent

Tissue-resident macrophages are key players in inflammatory processes, and their activation and functionality are crucial in health and disease. Numerous diseases are associated with alterations in homeostasis or dysregulation of the innate immune system, including allergic reactions, autoimmune diseases, and cancer. Macrophages are a prime target for drug discovery due to their major regulatory role in health and disease. Currently, the main sources of macrophages used for therapeutic compound screening are primary cells isolated from blood or tissue or immortalized or neoplastic cell lines (e.g., THP-1). Here, we describe an improved method to employ induced pluripotent stem cells (iPSCs) for the high-yield, large-scale production of cells resembling tissue-resident macrophages. For this, iPSC-derived macrophage-like cells are thoroughly characterized to confirm their cell identity and thus their suitability for drug screening purposes. These iPSC-derived macrophages show strong cellular identity with primary macrophages and recapitulate key functional characteristics, including cytokine release, phagocytosis, and chemotaxis. Furthermore, we demonstrate that genetic modifications can be readily introduced at the macrophage-like progenitor stage in order to interrogate drug target-relevant pathways. In summary, this novel method overcomes previous shortcomings with primary and leukemic cells and facilitates large-scale production of genetically modified iPSC-derived macrophages for drug screening applications.

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