scholarly journals Effect of Tamarindus indica juice intake on some oxidative stress markers in carbon tetrachloride induced rats

2021 ◽  
Vol 14 (1) ◽  
pp. 31-37
Author(s):  
N.S. Sadi ◽  
S.M. Abubakar ◽  
A. Ibrahim ◽  
A.M. Umar ◽  
A.M. Gadanya ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Normal Control ◽  
Colorimetric Assay ◽  
Normal Control Group ◽  
Control Group ◽  
Albino Rats ◽  
High Dose ◽  
Oxidative Stress Markers ◽  
Standard Drug ◽  
Stress Markers

Tamarind tree is a multipurpose tree of which almost every part finds at least some use, either nutritional or medicinal. Due to its pleasant acidic taste and rich aroma, the pulp is widely used for domestic and industrial purpose. A study was carried out to evaluate the effect of Tamarind juice intake in CCl4 induced oxidative stress albino rats. The Proximate, antinutrient, and Phytochemical contents of tamarind juice were analyzed using standard AOAC methods while mineral contents were determined using atomic absorption spectrometry. Oxidative stress markers were also analyzed using colorimetric assay kit. The serum levels of oxidative stress markers were compared between the normal and test groups. Experimental rats were divided into five groups: Normal control group, negative control (CCl4) group, standard drug (Vitamin C) group, tamarind low and high dose group. At the end of the experiment, significant increase in malondialdehyde level and decrease in superoxide dismutase, catalase, reduced glutathione and glutathione Peroxidase activities were recorded in CCl4-exposed rats as compared to normal control group. In the tamarind supplemented groups, the level of MDA along with the activities of SOD, CAT, GSH and GPx were comparable with the normal control rats (p>0.05). Thus, it appears that tamarind juice ameliorate the effect of CCl4; suggesting that consumption of natural compounds with an antioxidant profile may be a preventive alternative to those diseases associated with oxidative stress.

scholarly journals Auricularia Polytricha (Mushroom) Regulates Testicular DNA Expression and Oxidative Stress Markers of Streptozotocin-Induced Diabetic Male Wistar Rat

2020 ◽  
Vol 5 (3) ◽  
pp. 7-15
Author(s):  
Agbor Cyril Abang ◽  
Anyanwu G. E
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Normal Control ◽  
Diabetic Control ◽  
Normal Control Group ◽  
Control Group ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Testicular Dysfunction ◽  
Dna Strands

Local Nigerian men have been using AuriculariaPolytricha as a treatment for sexual dysfunction without supporting evidence from scientific experiments. This study was to investigate the effect of ethanolic extract of A. Polytricha on testicular DNA expression and some oxidative stress markers using STZ-Induced diabetic rats as a model. The experiment included six groups, Group A (Normal Control, treated with normal saline), Group B (treated with 65mg/kg.bw of STZ), Groups C, D, and E (treated with 250mg/kg.bw, 500mg/kg.bw, 1000mg/kg.bw AP after inducing diabetics), and Group F (treated with 40mg/kg.bw metformin after inducing diabetics). The experiment lasted for 35 days. After termination of the experiment, Fuelgen nuclear reaction was used for DNA demonstration to assess testicular DNA distribution while serum Superoxide Dimutase (SOD), Catalase and Melondialdehyde where evaluated using reagent based antioxidant enzyme assay. Results reveals that SOD and Melondialdehyde activities were remarkably (p<0.05) higher in diabetic control animals when compared with the normal control group. Values in Groups C, D and F that were administered with 250, 500mg/kg.bw A. polytricha and metformin respectively were also significantly (p<0.05) increased when compared with the normal control group. However, diabetic animals placed on 1000mg/kg.bw A. polytrichadid not show any statistical significance in comparison with normal control group but was remarkably (p<0.01) decreased when compared to the diabetic group that received low dose A. polytricha, an indication that the reversal is dose dependent. Catalase concentration in diabetic control animals was remarkably (p<0.05) higher when compared to the normal control but was not significantly (p<0.05) different in groups D (DM+500mg/kg.bw A. polytricha) and E (DM+1000mg/kg.bw A. polytricha) when compared with the normal control group. Diabetic control animals showed reduced magenta colour intensity of DNA and increased clustering and cross linking of DNA strands when compared with the normal control. However the degree of cross link in DNA strands was reduced in the diabetic animals placed on 1000mg/kg.bw A. polytrichawhen compared with the diabetic control group. Reversal in DNA damage and values of serum oxidative stress markers following administration of graded doses of A. polytricha could be attributed to essential phytochemical and therapeutic constituents in A. polytricha like polyphenol and flavonoid which can be found useful in prevention and treatment of diabetes induced testicular dysfunction. In summary, AP can contribute to a reversal in DNA damage and levels of serum oxidative stress markers in treating diabetes-induced testicular dysfunction.

scholarly journals ANALYSIS OF OXIDATIVE STRESS MARKERS IN CHRONIC CONSUMPTION OF MONOSODIUM GLUTAMATE ON LIVER OF WISTAR ALBINO RATS

2021 ◽  
pp. 116-119
Author(s):  
SURENDRA BABU THANGACHI ◽  
VARSHA SRIRAM MOKHASI ◽  
AGA AMMAR MURTHUZA
Keyword(s):  
Oxidative Stress ◽  
Monosodium Glutamate ◽  
Liver Homogenate ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Albino Rats ◽  
High Dose ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Wistar Albino Rats

Objective: The study was intended to explore whether Monosodium glutamate (MSG) induces oxidative stress on the liver of Wistar albino rats when fed chronically at three different doses, namely, low, mid, and high doses identical to human consumption doses in growing countries. Methods: The acclimatized Wistar albino rats (n=24) were randomly selected and grouped into four groups, namely Control, Low dose MSG (180 mg kg), Mid dose MSG (360 mg/kg), and High dose MSG (720 mg/kg). The animals were orally administered MSG for 120 days. After completion of the experimental period (120 days), euthanized animal liver was homogenized to investigate the oxidative stress marker enzymes such as Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), Catalase (CAT), and Myeloperoxidase (MPO). Results: The MPO showed a significant increase (p<0.05) in liver homogenate of all MSG induced groups when compared to control group. The SOD, CAT, and GPx activity deteriorated (p<0.05) in monosodium induced groups contrasting to the control group. Conclusion: The effects of MSG on oxidative stress markers on liver homogenate in the current study exhibited erratic abnormal changes in oxidative stress markers of monosodium induced groups which contemplate the harmful effects of MSG consumed chronically. The further studies should confirm the genetic basis of oxidative stress damage and transform the safety regulations of MSG consumption throughout the world.

scholarly journals Oxidative Stress Markers in Prostate Cancer Patients after HDR Brachytherapy Combined with External Beam Radiation

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Alina Woźniak ◽  
Rafał Masiak ◽  
Michał Szpinda ◽  
Celestyna Mila-Kierzenkowska ◽  
Bartosz Woźniak ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Prostate Cancer ◽  
External Beam Radiotherapy ◽  
External Beam Radiation ◽  
Control Group ◽  
High Dose ◽  
External Beam ◽  
Oxidative Stress Markers ◽  
Beam Radiation ◽  
Stress Markers

Assessment of oxidative stress markers was perfomed in prostate cancer (PCa) patients subjected to high-dose brachytherapy (HDR) with external beam radiotherapy (EBRT). Sixty men with PCa were subjected to combined two-fraction treatment with HDR (tot. 20 Gy) and EBRT (46 Gy). Blood samples were taken before treatment, immediately afterwards, after 1.5–3 months, and approx. 2 years. Control group consisted of 30 healthy men. Erythrocyte glutathione peroxidase activity in the patients was lower than in healthy subjects by 34% (, 50% (, 30% (, and 61% (, respectively, at all periods. No significant differences were found by comparing superoxide dismutase and catalase activity in PCa patients with that of the controls. After 2 years of the end of treatment, the activity of studied enzymes demonstrated a decreasing tendency versus before therapy. Blood plasma thiobarbituric acid reactive substances (TBARS) concentration was higher than in the controls at all periods, while erythrocyte TBARS decreased after 2 years to control levels. The results confirm that in the course of PCa, imbalance of oxidant-antioxidant processes occurs. The therapy did not alter the levels of oxidative stress markers, which may prove its applicability. Two years is too short a period to restore the oxidant-antioxidant balance.

scholarly journals Combined Antioxidant Recovery Properties of Fruit Juice Extracts of Cucumber and Water Melon on Lipid Profile and Gonadal Steroid Levels of Cadmium Induced Testicular Damage on Male Albino Rats

2019 ◽  
pp. 1-9
Author(s):  
F. C. Anacletus ◽  
B. Nwakaku ◽  
K. T. Nwauche
Keyword(s):  
Normal Control ◽  
Low Dose ◽  
Fruit Juice ◽  
Positive Control ◽  
Normal Control Group ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Albino Rats ◽  
High Dose ◽  
Treatment Groups

The antioxidant protective effects of fruit juice of cucumber and watermelon on lipid profile of cadmium induced toxicity on male albino rats was investigated. Forty male rats were divided into eight groups. Group NC served as normal control group while group PC was positive control that was not treated but induced with cadmium. Groups I to VI received high dose and low dose of juice of Cucumber and Watermelon respectively. Excluding the normal control group, other groups were fed with lard 14 days before treatment commenced.  Doses of 0.8 mg/kg-high dose and 0.4 mg/kg-low dose for cucumber and watermelon respectively. At the 4th and 6th week, biochemical parameters were assayed. Results revealed that the levels of total cholesterol, LDL, VLDL and triglyceride significantly (P˂0.05) were decreased compared to positive control but HDL was increased in treatment groups compared to positive control. Pretreatment with cucumber and watermelon juice indicated that total cholesterol, LDL, VLDL and triglyceride significantly (P˂0.05) were decreased compared to positive control but HDL was increased in treatment groups compared to positive control. The result also revealed an increase in testosterone levels in treated groups after 4 weeks of administration of whole extract of cucumber and watermelon when compared to their week 2 values. Testosterone level in positive control was also reduced significantly from 1.5±0.14 ng/ml to 0.46±0.31 ng/ml. Histological evaluation of the testes of normal control group revealed that the interstitium was intact with leydig cells present and maturing germ cells embedded in normal seminiferous tubules while the other groups that were induced with cadmium only showed morphology of testes with empty seminiferous tubules and consolidated interstitial spaces.

Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Type 2 Diabetes Mellitus: A Retrospective Cohort Study

2020 ◽  
Vol 20 (4) ◽  
pp. 584-590 ◽  
Author(s):  
Shima Fathi ◽  
Shiva Borzouei ◽  
Mohammad Taghi Goodarzi ◽  
Jalal Poorolajal ◽  
Fatemeh Ahmadi-Motamayel
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Control Group ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Type 2 Dm ◽  
Patients With Diabetes ◽  
The Difference ◽  
And Oxidative Stress

Background: Diabetes Mellitus (DM) is a progressive metabolic disorder. Objective: The aim of this study was to investigate the relationship between antioxidant and oxidative stress markers in the saliva of patients with type 2 DM and a healthy control group. Methods: In this study, 20 patients with diabetes and 20 healthy individuals were evaluated. Salivary antioxidants markers consisted of total antioxidant capacity (TAC), uric acid (UA), peroxidase and catalase. Oxidative stress markers included total oxidant status (TOS), malondealdehyde (MDA) and total thiol (SH). Sialochemical analysis was performed with spectrophotometric assay. All the statistical analyses were conducted using STATA software. Results: TAC decreased significantly in patients with diabetes. Although salivary UA and peroxidase were lower in patients with diabetes compared to the control group, the difference was not significant. Salivary catalase in patients with diabetes was significantly lower than that in the control group. MDA and TOS exhibited significantly higher levels in type 2 DM. SH levels were slightly higher in DM. Conclusions: According to the results of the present study, there were some changes in the salivary levels of some antioxidants and oxidative stress markers in patients with type 2 DM and could be measured as an indicator of serum changes..

scholarly journals The Effects of Vibroacoustically Induced Microvibrations on Arterial Blood Pressure and Oxidative Stress in Rats

2014 ◽  
Vol 15 (2) ◽  
pp. 83-88
Author(s):  
Dusko Kornjaca ◽  
Vladimir Zivkovic ◽  
Nevena Barudzic ◽  
Vladimir Jakovljevic ◽  
Dragan Djuric
Keyword(s):  
Oxidative Stress ◽  
Arterial Pressure ◽  
Systolic Arterial Pressure ◽  
Control Group ◽  
Oxidative Stress Markers ◽  
Sound Waves ◽  
Initial Value ◽  
The Third ◽  
Stress Markers ◽  
Arterial Blood

ABSTRACT Vibroacoustics, a scientific field that has been intensively studied for the last thirty years, uses the properties of sound waves (infrasound, ultrasound, noise and music) to induce vibrations that, like a sound wave, may have both useful and harmful effects. Th e aim of this study was to examine the effects of vibroacoustically induced microvibrations on arterial blood pressure and markers of oxidative stress in the blood. Th e experiments were performed on Wistar male rats that had a 180-200 g body mass and were divided into control and experimental groups (6 rats in each). In the experimental group, microvibrations were induced using the Vitafon vibroacoustic apparatus (Vitafon, St. Petersburg, Russian Federation), which delivers sound waves of varying frequencies by a process called “phoning”. Up to 60 minutes of phoning time was delivered to the kidney and liver using 4 diff erent regimens that included a 5-minute stabilisation time; up to four 10-minute phoning regimens, with 5-minute breaks between each single regimen, at a 30 Hz-18000 kHz frequency range;, and 2.8 μm-12.3 μm microwave amplitudes. After the completion of a phoning regimen, animals were sacrificed and the oxidative stress markers were measured in blood samples (O2-, H2O2, nitrites, lipid peroxidation index, superoxide dismutase, catalase, and glutathione) and compared with the values of markers in the control group. Systolic arterial pressure was analysed after the acute application of up to four diff erent regimens of vibroacoustic microvibrations. Systolic arterial pressure decreased significantly during the administration of the second regimen in comparison to the control group. Systolic arterial pressure returned, almost completely, to the initial value after the administration of the third and fourth regimens. Th ere was no significant change in diastolic arterial pressure after the acute administration of up to four different regimens, although the pressure decreased slightly after the first and second regimens and returned to the initial value during the administration of the third and fourth regimens. Analysis of oxidative stress markers showed a statistically significant change in the catalase level. No statistically significant differences were found in the other oxidative stress markers analyzeanalysed. Further research is needed to clarify the physiological effects of low compared to high frequencies of vibroacoustically induced microvibrations and their possible therapeutic significance.

scholarly journals The Effect of High-Dose Insulin Analog Initiation Therapy on Lipid Peroxidation Products and Oxidative Stress Markers in Type 2 Diabetic Patients

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Hazal Tuzcu ◽  
Ibrahim Aslan ◽  
Mutay Aslan
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Blood Glucose ◽  
Glucose Variability ◽  
Insulin Analog ◽  
High Dose ◽  
Oxidative Stress Markers ◽  
Insulin Analogs ◽  
Stress Markers ◽  
Mean Blood Glucose

Effect of high-dose insulin analog initiation therapy was evaluated on lipid peroxidation and oxidative stress markers in type 2 diabetes mellitus (T2DM). Twenty-four T2DM patients with HbA1c levels above 10% despite ongoing therapy with sulphonylurea and metformin were selected. Former treatment regimen was continued for the first day followed by substitution of sulphonylurea therapy with different insulin analogs. Glycemic profiles were determined over 72 hours by Continuous Glucose Monitoring System (CGMS), and blood/urine samples were collected at 24 and 72 hours. Insulin analog plus metformin treatment significantly reduced glucose variability. Plasma and urine lipid peroxidation were markedly decreased following insulin analog plus metformin treatment. No correlation existed between glucose variability and levels of plasma and urine oxidative stress markers. Likewise, changes in mean blood glucose from baseline to end point showed no significant correlation with changes in markers of oxidative stress. On the contrary, decreased levels of oxidative stress markers following treatment with insulin analogs were significantly correlated with mean blood glucose levels. In conclusion, insulin plus metformin resulted in a significant reduction in oxidative stress markers compared with oral hypoglycemic agents alone. Data from this study suggests that insulin analogs irrespective of changes in blood glucose exert inhibitory effects on free radical formation.

scholarly journals Swimming Attenuates Blood Pressure and Oxidative Stress in Hypertensive Rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Anica Petkovic ◽  
Marko Ravic ◽  
Sasa Plecevic ◽  
Jovana Jeremic ◽  
Ivan Srejovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Blood Pressure ◽  
Diastolic Pressure ◽  
Control Group ◽  
Albino Rats ◽  
Positive System ◽  
Hypertensive Rats ◽  
Stress Markers ◽  
Antioxidant Parameters ◽  
And Oxidative Stress

Abstract Hypertension presents one of the main risk factors for cardiovascular diseases which are the leading cause of morbidity and mortality worldwide. Structural and mechanical changes of the heart and blood vessels as well as overproduction of reactive oxygen species may occur due to the increased blood pressure. Therewith, the goal of our study was to estimate the effects and duration of swimming as a possible therapy approach on blood pressure and oxidative stress parameters in normotensive and hypertensive rats. The study was conducted on 60 male Wistar albino rats divided into two groups, normotensive and hypertensive rats. Each of these groups was divided into three subgroups according to the swimming protocol. The swimming training was kept constant (60 min/day, for five days a week) with two days of rest. After six or nine weeks of the swimming protocol, blood pressure and oxidative stress markers were measured. The control group rats were put in water for one minute a day, in order to avoid water-induced stress. Training significantly reduced systolic blood pressure in hypertensive rats, while diastolic pressure did not change in the group that swam six or nine weeks. The results showed that swimming increases the activity of all measured antioxidative parameters, while values of prooxidants varied depending on the training protocol. Our results confirmed that swimming, as an aerobic exercise, decreases blood pressure and has time-dependent positive system adaptations, especially on the antioxidant parameters.

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