scholarly journals In vivo antidiabetic activity of qwueous extract of Artemisia argyi (Chinese mugwort) in alloxan-induced diabetic rats

2020 ◽  
Vol 19 (7) ◽  
pp. 1487-1493
Author(s):  
Xinliang Wu ◽  
Junxue Zhuang ◽  
Zixia Bai ◽  
Daliang Guo
Keyword(s):  
Diabetes Mellitus ◽  
Total Cholesterol ◽  
Histological Examination ◽  
Glycosylated Hemoglobin ◽  
Intraperitoneal Administration ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Hypolipidemic Effect ◽  
Artemisia Argyi ◽  
Soxhlet Apparatus

Purpose: To determine the antidiabetic, antioxidant and anti-hyperlipidemic effects of aqueous leaf extract of Artemisia argyi (Asteraceae) in alloxan (ALX)-induced diabetic rats. Experimental: Soxhlet apparatus was packed with grinded leaves of A. Argyi and subjected to extraction by double distillation using water as  running solvent for 4 – 5 h. Male albino Wistar rats weighing 150 ± 10 g were used in this study. Diabetes was induced in overnight-fasted rats via intraperitoneal administration of freshly prepared 10 % alloxan solution at a dose of 186.9 mg/kg. Serum glucose (Glc), high-density lipoprotein cholesterol (HDL-c), triglycerides (TGs) and total cholesterol (TC) were evaluated using Randox assay kits. Serum reduced glutathione (GSH) was assayed using a slight modification of a previously reproted procedure, while histological examination was carried out microscopically after hematoxylin and eosin staining. Results: Oral administration of aqueous extract of Artemisia argyi significantly reduced ALX-induced increases in glycosylated hemoglobin and blood glucose, but significantly increased total protein, hemoglobin, insulin, and C-peptide levels (p < 0.05). Administration of the extract also led to a significant upsurge in non-enzymic antioxidants i.e. ceruloplasmin, GSH, vitamin E and vitamin C. The extract produced a hypolipidemic effect by significantly reducing total cholesterol (TC) and serum TGs. The hypoglycemic and hypolipidemic effects of the extract were dose-dependent (p < 0.05). Histological examination of the pancreas revealed that the extract protected the integrity of beta cells in ALXinduced diabetic rats. Conclusion: These results indicate the beneficial effects of Artemisia argyi against diabetes mellitus. Thus, Artemisia argyi may be useful in the management of diabetes mellitus. Keywords: Artemisia argyi, Antidiabetic, Glutathione, Histopathology, Antioxidant

ANTIDIABETIC ACTIVITY OF METHANOLIC EXTRACT OF CUCUMIS DIPSACEUS EHRENB. (FRUIT) IN STREPTOZOTOCIN- INDUCED DIABETIC RATS

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (12) ◽  
pp. 53-61
Author(s):  
Gagandeep Kaur ◽  
◽  
D. Srivastav ◽  
Suman Lata
Keyword(s):  
Blood Glucose ◽  
Total Cholesterol ◽  
Cholesterol Level ◽  
Methanolic Extract ◽  
Total Cholesterol Level ◽  
Serum Insulin ◽  
Glycosylated Hemoglobin ◽  
Diabetic Rats ◽  
The Body ◽  
Antidiabetic Activity

The present study was designed to investigate the hypoglycemic and antidiabetic properties of Cucumis dipsaceus fruit which is widely used in the Indian traditional medicine for the treatment of diabetes.Aim of present study, to investigate the antidiabetic action of the methanolic extract of C. dipsaceus fruits in Streptozotocin induced-diabetic rats. The antidiabetic activity of methanolic extract of fruit C. dipsaceus was evaluated by using normal and streptozotocin induced-diabetic rats. The acute effect of methanolic extract was evaluated by administering 500 mg/kg p.o. to normoglycemic rats. In the chronic model, the methanolic extract was administered to normal and STZ-induced-diabetic rats at dose of 250 mg/kg and 500 mg/kg p.o. per day for 21 days. Blood glucose levels and body weights were observed at specific intervals and different biochemical parameters were also monitored. Methanolic extract (500mg/kg) produced a significant reduction in blood glucose level in STZ- induced diabetic rats. The statistical data indicated a significant increase in the body weight and serum insulin level and decrease in the glycosylated hemoglobin levels. Total cholesterol level decreased while an increase in HDL and LDL cholesterol level was observed when treated with the extract. Thus the methanolic fruit extract of C. dipsaceus exhibited significant anti-hyperglycemic activities and beneficial effects in reducing the elevated total cholesterol level of STZ-induced-diabetic rats.

scholarly journals Evaluation of Antidiabetic and Antihyperlipidemic Effects of Peganum harmala Seeds in Diabetic Rats

Cholesterol ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Gholamreza Komeili ◽  
Mohammad Hashemi ◽  
Mohsen Bameri-Niafar
Keyword(s):  
Total Cholesterol ◽  
Glycosylated Hemoglobin ◽  
Diabetic Rats ◽  
Male Rats ◽  
Peganum Harmala ◽  
Hydroalcoholic Extract ◽  
End Of Treatment ◽  
Total Antioxidant ◽  
Treatment Of Diabetes ◽  
Different Doses

The present study was carried out to investigate the antidiabetic and antihyperlipidemic properties of hydroalcoholic extract of Peganum harmala in streptozotocin-induced diabetic male rats. In an experimental study, 64 normal Wistar albino male rats (200–230 g) were randomly divided into 8 groups. Control and diabetic rats were treated with normal saline and three different doses (30, 60, and 120 mg/kg) of hydroalcoholic extract of Peganum harmala seeds for 4 weeks orally. At the end of treatment, blood samples were taken and glucose, triglycerides, total cholesterol, LDL-c, HDL-c, malondialdehyde (MDA), total antioxidant capacity (TCA), ALT, AST, GGT, bilirubin, and glycosylated hemoglobin (HbA1C) were determined. STZ-induced diabetic rats showed significant changes in the values of glucose, triglycerides, total cholesterol, LDL-c, MDA, TAC, ALT, AST, GGT, bilirubin, and HbA1C in comparison with normal rats. Administration of the extract to diabetic rats resulted in a remarkable decrease in glucose, lipid profiles, MDA, ALT, AST, GGT, bilirubin, and HbA1C levels and increase in TAC relative to diabetic group. The results of this study indicated that hydroalcoholic extract of Peganum harmala seeds possesses antidiabetic and hypolipidemic activities and could be useful in treatment of diabetes.

scholarly journals Anti-Diabetic Potential of the Leaves of Anisomeles malabarica in Streptozotocin Induced Diabetic Rats

2017 ◽  
Vol 43 (4) ◽  
pp. 1689-1702 ◽  
Author(s):  
Peddanna Kotha ◽  
Kameswara Rao Badri ◽  
Ramya Nagalapuram ◽  
Rajasekhar Allagadda ◽  
Appa Rao Chippada
Keyword(s):  
Diabetes Mellitus ◽  
Glucose Tolerance ◽  
Ethyl Acetate ◽  
Plasma Insulin ◽  
Ethyl Acetate Extract ◽  
Glycosylated Hemoglobin ◽  
Diabetic Rats ◽  
Active Fraction ◽  
Insulin Levels ◽  
Plasma Insulin Levels

Background/Aims: Diabetes mellitus is a pandemic metabolic disorder that is affecting a majority of populations in recent years. There is a requirement for new drugs that are safer and cheaper due to the side effects associated with the available medications. Methods: We investigated the anti-diabetic activity of leaves of Anisomeles malabarica following bioactivity guided fractionation. The different solvent (hexane, ethyl acetate, methanol and water) extracts of A. malabarica leaves were used in acute treatment studies to evaluate and identify the active fraction. The ethyl acetate extract was subjected to further fractionation using silica gel column chromatography and the compounds were identified by LC-SRM/MS and GC-MS. Additional chronic treatment studies were carried out using this active fraction (AMAF) for 30 days in experimental diabetic rats. Fasting blood glucose (FBG), glycosylated hemoglobin (HbA1c), plasma insulin levels and glucose tolerance were measured along with insulin resistance/sensitivity indicators (HOMA-IR, HOMA-β and QUICKI) to assess the beneficial effects of A. malabarica in the management of diabetes mellitus. Results: Among the different solvent extracts tested, ethyl acetate extract showed maximum (66%) anti-hyperglycemic activity. The hexane and ethyl acetate (1: 1) fraction that has maximum anti-diabetic activity was identified as active fraction of A. malabarica (AMAF). The FBG, HbA1c, plasma insulin levels and insulin sensitivity/resistance indicators such as glucose tolerance, HOMA-IR, HOMA-β and QUICKI were significantly improved to near normal in diabetic rats treated with AMAF. Further, we identified key flavonoids and fatty acids as the anti-diabetic active principles from the AMAF of A. malabarica leaves. Conclusion: The results of our study suggest that Anisomeles malabarica has potential anti-diabetic activity in STZ induced diabetic rats.

scholarly journals Evaluation of Antidiabetic Activity of Gymnema sylvestre and Andrographis paniculata in Streptozotocin Induced Diabetic Rats

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Prem Kumar ◽  
Sudha Rani ◽  
B Arunjyothi ◽  
P. Chakrapani ◽  
A Rojarani
Keyword(s):  
Diabetes Mellitus ◽  
Medicinal Plants ◽  
Low Cost ◽  
Diabetic Rats ◽  
Andrographis Paniculata ◽  
Antidiabetic Activity ◽  
Gymnema Sylvestre ◽  
Streptozotocin Induced Diabetes ◽  
Oxidative Effect

Diabetes mellitus is a difficult metabolic disorder that has seriously impact the human health and quality of life. Medicinal plants are being used to control diabetes However, they are not entirely effective and no one has ever been reported to have fully recovered from diabetes. Many plants have been used for the management of diabetes mellitus in various traditional systems of medicine worldwide as they are a great source of biological constituents and many of them are known to be effective against diabetes. Medicinal plants with antihyperglycemic activities are being more desired, owing to lesser sideeffects and low cost. Streptozotocin was induced to all groups of rats at dosage of 35 -55mg/kg except for the normal. Streptozotocin induced diabetes in sprague dawly rats were used to study antidiabetic activity of methonolic extract of two medicinal plants Gymnema sylvestre,Andrographis paniculata methanolic leaf extract was administered orally in graded doses of 30 mg/kg,50mg /kg sprague dawly rats Gymnema sylvestre at a dose of 30mg/kg and Andrographis paniculata at a dose of 50mg/kg showed significant anti-hyperglycemic and anti-oxidative effect which was evident from the 1st week of treatment.

scholarly journals Effects of Orally Consumed Rosa damascena Mill. Hydrosol on Hematology, Clinical Chemistry, Lens Enzymatic Activity, and Lens Pathology in Streptozotocin-Induced Diabetic Rats

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4069
Author(s):  
İlker Demirbolat ◽  
Cansu Ekinci ◽  
Fadime Nuhoğlu ◽  
Murat Kartal ◽  
Pelin Yıldız ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Enzymatic Activity ◽  
Aldose Reductase ◽  
Clinical Chemistry ◽  
Intraperitoneal Administration ◽  
Diabetic Rats ◽  
Rosa Damascena ◽  
Dependent Manner ◽  
Chronic Hyperglycemia ◽  
Dose Dependent

Diabetes mellitus is a multisystemic metabolic disorder that may affect the eyes, kidneys, vessels, and heart. Chronic hyperglycemia causes non-enzymatic glycation of proteins and elevation of the polyol pathway resulting in oxidative stress that damages organs. The current study aimed to investigate the dose-dependent effects of orally consumed Rosa damascena Mill. hydrosol on hematology, clinical biochemistry, lens enzymatic activity, and lens pathology in streptozotocin (STZ)-induced diabetic rats. Diabetes was induced into male Sprague–Dawley rats by intraperitoneal administration of STZ (40 mg/kg body weight). Rose hydrosols containing 1515 mg/L and 500 mg/L total volatiles (expressed as citronellol) were introduced to rats orally for 45 days. Consumption of 1515 mg/L volatile containing rose hydrosol successfully ameliorated hematologic, hepatic, and renal functions. Hydrosols also attenuated hyperglycemia and decreased the advanced glycation end-product formation in a dose-dependent manner. Rose hydrosol components significantly increased the lens enzymatic activities of glutathione peroxidase and decreased the activity of aldose reductase to prevent cataractogenesis. Histopathological examinations of rat lenses also indicated that increasing the dose of rose hydrosol had a protective effect on lenses in diabetic conditions. Additionally, in silico modeling of aldose reductase inhibition with rose hydrosol volatiles was carried out for extrapolating the current study to humans. The present results suggest that rose hydrosol exerts significant protective properties in diabetes mellitus and has no toxic effect on all studied systems in healthy test groups.

scholarly journals An antioxidant activity of Cinnamonum tamala improves histopathological alterations and biochemical parameters in alloxan induced diabetic rats

2019 ◽  
Vol 10 (6) ◽  
pp. 50-56 ◽  
Author(s):  
Mamata Laxmikant Pochhi
Keyword(s):  
Serum Insulin ◽  
Glycosylated Hemoglobin ◽  
Antioxidant Properties ◽  
Insulin Level ◽  
Diabetic Control ◽  
Protective Role ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Standard Drug ◽  
Cinnamomum Tamala

Background: Diabetic mellitus is a multifactorial disorder associated with its devastating consequences has assumed epidemic proportion. Diabetes mellitus (DM) is a global health problem and the incidence of DM is increasing at alarming rate all over the world. Many Indian medicinal plants have been reported to possess potential antidiabetic activity and could play important role in the management of diabetes with less adverse effects. Aims and Objectives: The main objective of this study was to focus on the anti-diabetic activity of Cinnamomum tamala, with special reference to its curative and protective role in alloxan induced diabetic rats. Attempts were further made to study the antioxidant properties of C. tamala leaves. Materials and Methods: The diabetic rats were administered orally with the aqueous leaves extracts of Cinnamonum tamala (250 mg/kg) for 30 days. The results were compared with standard drug Tolbutamide. Result: The alloxan treated diabetic control rats showed a significant increase in the plasma glucose, glycosylated hemoglobin (HbA1C), glucose-6-phosphatase, aldolase, LDH, ALT, AST, ALP and GGT activity, freeradicals formation with a concomitant decrease in glycogen content in the liver and serum insulin level and phosphoglucoisomerase and hexokinase activity in tissues as compared to normal control rats. Oral administration of C. tamala extract for 30 days showed significant result as compared to Tolbutamide and diabetic control rats. Conclusion: On the basis of above findings it can be concluded that extracts of C. tamala to alloxan induced diabetic rats showed significant positive changes in the biochemical and histopathological parametersrelated to carbohydrate and protein metabolism.

scholarly journals Antidiabetic Effects of the Ethanolic Root Extract ofUvaria chamaeP. Beauv (Annonaceae) in Alloxan-Induced Diabetic Rats: A Potential Alternative Treatment for Diabetes Mellitus

2018 ◽  
Vol 2018 ◽  
pp. 1-13
Author(s):  
Jonathan Emeka Emordi ◽  
Esther Oluwatoyin Agbaje ◽  
Ibrahim Adekunle Oreagba ◽  
Osede Ignis Iribhogbe
Keyword(s):  
Diabetes Mellitus ◽  
Alternative Treatment ◽  
Pancreatic Beta Cells ◽  
Hematological Parameters ◽  
Hdl Cholesterol ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Root Extract ◽  
Acacia Senegal ◽  
Cholesterol Levels

Diabetes mellitus has been a menace to mankind from time immemorial. However, a natural product such asU. chamaeP. Beauv (Annonaceae) offers alternative treatment for diabetes mellitus. The study aimed at evaluating antidiabetic activity of the ethanolic root extract ofU. chamaein alloxan-induced diabetic rats. Diabetes was induced in Sprague Dawley rats after overnight fast with 150 mg/kg alloxan intraperitoneally. After 72 h, those with plasma glucose levels >200 mg/dl were classified as diabetic. Five diabetic rats in each group were treated daily for 14 days orally with 100, 250, and 400 mg/kg of the extract, glibenclamide (71 µg/kg) and pioglitazone (429 µg/kg), respectively, while another group was untreated. Control received 0.5 ml ofAcacia senegal. Effects of extract on glucose, other biochemical, and hematological parameters were evaluated.α-amylase andα-glucosidase inhibitory activities of extract and its fractions were also evaluated. Percentage inhibition and IC50values were determined. Diabetic control was achieved on the 7th day of the study with 100, 250, and 400 mg/kg of the extract showing glucose reduction of 72.14%, 78.75%, and 87.71%, respectively. The HDL-cholesterol levels of diabetic rats treated with extracts were significantly increased. Extract and its fractions causedα-amylase andα-glucosidase inhibition. Histologically, pancreas of diabetic rats treated with extract showed regenerated islet cells which were not seen in rats treated with glibenclamide and pioglitazone. This study showed thatU. chamaehas antidiabetic activity which may be throughα-amylase andα-glucosidase inhibition and regeneration of pancreatic beta cells. Also, it may reduce the risk of cardiovascular disease by increasing HDL-cholesterol levels.

scholarly journals Investigation of the In Vitro Antioxidant Potential Of Polyphenolic-Rich Extract of Artocarpus heterophyllus Lam Stem Bark and Its Antidiabetic Activity In Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 25 ◽  
pp. 2515690X2091612 ◽  
Author(s):  
Basiru Olaitan Ajiboye ◽  
Oluwafemi Adeleke Ojo ◽  
Babatunji Emmanuel Oyinloye ◽  
Mary Abiola Okesola ◽  
Adeyonu Oluwatosin ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Fasting Blood Glucose ◽  
Stem Bark ◽  
Diabetic Rats ◽  
Antioxidant Potential ◽  
Antidiabetic Activity ◽  
Artocarpus Heterophyllus ◽  
Inflammatory Parameters ◽  
Anti Inflammatory

Artocarpus heterophyllus Lam (Moraceae) stem bark has been used locally in managing diabetes mellitus with sparse scientific information. This study investigates the in vitro antioxidant potential of polyphenolic-rich extract of A heterophyllus stem bark as well as its antidiabetic activity in streptozotocin-induced diabetic rats. Fifty male Wistar rats were used with the induction of diabetes by a single intraperitoneal injection of streptozotocin (45 mg/kg body weight) and were orally administered 400 mg/kg free and bound phenols of A heterophyllus stem bark. The animals were sacrificed on the 28th day of the experiment using the cervical dislocation method; antihyperglycemia and anti-inflammatory parameters were subsequently assessed. The polyphenolic extracts demonstrated antioxidant potentials (such as hydrogen peroxide and diphenyl-1-picrylhydrazyl), as well as strong inhibitory activity against amylase and glucosidase. There was a significant ( P < .05) increase in glycogen, insulin concentration, pancreatic β-cell scores (HOMA-β), antioxidant enzymes and hexokinase activities, as well as glucose transporter concentration in diabetic animals administered the extracts and metformin. Also, a significant ( P < .05) reduction in fasting blood glucose, lipid peroxidation, glucose-6-phosphatase, and all anti-inflammatory parameters were observed in diabetic rats administered the extracts and metformin. The extracts demonstrated antidiabetic potential, which may be useful in the management of diabetes mellitus

scholarly journals Antidiabetic effect of Coccinia grandis (L.) Voigt (Cucurbitales: Cucurbitaceae) on streptozotocin induced diabetic rats and its role in regulating carbohydrate metabolizing enzymes

2018 ◽  
Vol 5 (11) ◽  
pp. 683-698
Author(s):  
Meenatchi Packirisamy ◽  
Purushothaman Ayyakkannu ◽  
Maneemegalai Sivaprakasam
Keyword(s):  
Glycosylated Hemoglobin ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Perennial Herb ◽  
Optimum Dose ◽  
Oral Glucose ◽  
Metabolizing Enzymes ◽  
Coccinia Grandis ◽  
Treatment Of Diabetes

Coccinia grandis (L.) Voigt (Cucurbitales: Cucurbitaceae) is a climbing perennial herb, growing throughout India and it is widely used in the traditional treatment of diabetes. The aim of present study was to evaluate the antidiabetic potential of the mature unripe of Coccinia grandis in streptozotocin (STZ)-induced diabetic rats with special reference to carbohydrate metabolizing enzymes. The optimum dose of Coccinia grandis extract (GCE) was determined by oral glucose tolerance test. The effects of CGE were compared with glibenclamide. Oral administration of Coccinia grandis extract at a concentration of 250 mg/kg body weight once daily to diabetic rats for the period of 30 days resulted in significant reduction in the levels of plasma glucose and glycosylated hemoglobin. Administration of CGE showed a significant increase in the levels of glycolytic enzymes and glycogen content and decrease in the levels of gluconeogenic enzymes in the liver of diabetic treated rats. The anti-hyperglycemic effect of the extract was comparable with glibenclamide, a known hypoglycemic drug. Present findings provide experimental evidence that the fruits of C. grandis have potential antidiabetic activity which might be used as a functional food and safe remedy for the treatment of diabetes and associated complications.

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