Changes in the Microflora of Bovine Colostrum During Natural Fermentation

1976 ◽  
Vol 39 (1) ◽  
pp. 27-31 ◽  
Author(s):  
T. L. THOMPSON ◽  
E. H. MARTH
Keyword(s):  
Titratable Acidity ◽  
Bovine Colostrum ◽  
Coliform Bacteria ◽  
Aerobic Bacteria ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Natural Fermentation ◽  
Fecal Streptococci ◽  
Yeasts And Molds

Colostrum from five cows was collected and pooled separately and allowed to ferment naturally for 21 days. During this time the pH decreased and was approximately 4.0 at the end of the fermentation. The titratable acidity of the colostrum at this time was 1.5% or more. Numbers of total aerobic bacteria, fecal streptococci, coliform bacteria, and gram-negative bacteria increased rapidly during the first 2–3 days of fermentation. After several days of reasonable stability, numbers of aerobic bacteria and coliform bacteria declined, whereas numbers of fecal streptococci and gram-negative bacteria remained fairly constant. The number of yeasts and molds increased much more slowly than did those of bacteria and in some instances growth of these organisms was still evident when the fermentation was terminated.

Changing trends in bacteriology of peritonsillar abscess

2007 ◽  
Vol 122 (9) ◽  
pp. 928-930 ◽  
Author(s):  
S B Megalamani ◽  
G Suria ◽  
U Manickam ◽  
D Balasubramanian ◽  
S Jothimahalingam
Keyword(s):  
Tertiary Care ◽  
Peritonsillar Abscess ◽  
Aerobic Bacteria ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Increasing Trend ◽  
Changing Patterns ◽  
Sensitivity Studies ◽  
Resistance To Penicillin ◽  
Empirical Antibiotics

AbstractObjectives:To evaluate the type of aerobic bacteria commonly isolated from peritonsillar abscesses.Study design:A retrospective study involving 60 patients presenting with peritonsillar abscess treated between 2003 and 2006.Setting:The patients were treated in a tertiary care, teaching hospital. The records of the patients were reviewed and analysed.Results:Gram-positive organisms were commonly isolated from the abscesses. However, an increasing trend towards Gram-negative bacterial isolates was noted. The antibiogram showed changing patterns of sensitivity, with a resistance to penicillin.Conclusion:There is an increasing incidence of Gram-negative aerobic bacteria causing peritonsillar abscess. Appropriate antibiotics for Gram-negative bacteria should be included in the regimen if such bacteria are isolated in culture and sensitivity studies.Significance:Empirical antibiotics with Gram-negative cover will be appropriate as adjunctive therapy.

Identification and Drug Sensitivity of Aerobic Bacterial Isolates from Diabetic Foot Ulcers of Sudanese Patients: a Cross sectional study

2020 ◽  
Author(s):  
saada Mohamed nour ◽  
Maowia Mukhtar ◽  
Mohamed Elmakk ◽  
Elshibli Mohamed Elshibli ◽  
Walyeldin Elnour Elfakey ◽  
...  
Keyword(s):  
Diabetic Foot ◽  
Diffusion Method ◽  
Foot Ulcers ◽  
Aerobic Bacteria ◽  
Diabetic Patients ◽  
Gram Negative Bacteria ◽  
Female Ratio ◽  
Gram Negative ◽  
The Mean ◽  
Grade 3

Abstract Background Diabetic foot ulcer infection cause great morbidity and mortality among diabetic patients and is a major cause of lower extremity amputation worldwide. This study aimed to determine the profile of aerobic bacteria and their antibiotic sensitivity patterns in diabetic foot infections (DFI) among different Wagner's grades. Methods This study was conducted during December 2017 - March 2018 in a Diabetic Center, Sudan. A total of 152 diabetic patients with different grades of foot ulcers were randomly enrolled in the study. The patients were grouped using Wagner's classification. Tissue biopsies and deep swabs were collected from the ulcers for aerobic cultures. The cultured isolates were identified using phenotypic and biochemical properties and their sensitivity to commonly used antibiotics, Colistin, Aikacin, Ciprofloxacin, Augmentin, Ceftazideme, Gentamicin, Clindamycin, Ceftriaxone Meropenum. Cotrimoxazole, Erythomycin, Oxacillin and Vancomycin. Fusidic acid, Imepenem, and Piperacillin was tested using the Kirby Bauer disk diffusion method. Results The mean age of the patients was 54.31 (SD ± 12.1) years, male to female ratio of 8: 1. The mean duration of diabetes was 14 (SD ± 8) years. The ulcers varied in duration from 1 day to 10 years. of 152 samples 181 aerobic bacteria were isolated. Cultures yielded 1-3 isolate per culture. The maximum number was isolated from grade 3 group followed by long standing ulcer LSU group 50.8% and 28% respectively. Polymicrobial infection was higher in LSU (30.4%). The isolates were mostly Gram-negative bacteria. The most frequent were proteus spp. (35.3%), S. aureus MRSA 14.4% and Coliform 12.2% respectively. The most common isolates in grade 3 were P. Mirablis, Staphylococcus and Coliform and in long standing ulcers were P. Mirablis, S. aureus MRSA and Coagulase negative staphylococcus respectively. Conclusion Gram-negative bacteria were more prevalent and the most frequent pathogens were Proteus spp. The most common polymicrobial infections were due to P. mirablis with; P. aerginosa, S. MRSA and Coliform respectively . Gram negative rods were sensitive to Amikacin, (80.6) %) while the highest sensitivity of Gram positive was to Imepnem (85%). Most of the isolates were sensitive to Meropenem. No significant relation between Wagner grades and neuropathy was detected.

Potato-Like Odor of Retail Beef Cuts Associated with Species of Pseudomonas

1986 ◽  
Vol 49 (4) ◽  
pp. 272-273 ◽  
Author(s):  
R. L. DAISE ◽  
E. A. ZOTTOLA ◽  
R. J. EPLEY
Keyword(s):  
Coliform Bacteria ◽  
Plate Count ◽  
Aerobic Bacteria ◽  
Gram Negative ◽  
Consumer Complaints ◽  
Psychrotrophic Bacteria ◽  
Plate Count Agar ◽  
Litmus Milk ◽  
Musty Odor ◽  
Retail Cuts

Retail cuts of beef and hamburger packages from a North Dakota meat processor were examined due to consumer complaints of a strong potato-like or musty odor associated with the meat. Examination for total numbers of aerobic bacteria on plate count agar and for gram-negative psychrotrophic bacteria on crystal violet tetrazolium agar revealed numbers in excess of 108 CFU/g. Numbers of coliform bacteria on violet red bile agar were in excess of 106 CFU/g. Gram-negative rods were isolated and identified. The isolates were characterized by a positive catalase reaction, oxidase production, an oxidative O/F reaction, nonutilization of lactose, liquefication of nutrient gelatin, slight motility, production of acid in litmus milk with decoloration and clotting, nonproduction of indole, and nonreduction of nitrate. The isolate was tentatively identified as a Pseudomonas of undetermined species, probably a variant of either Pseudomonas taetrolens or Pseudomonas perolens.

Influence of Sodium Substitution with Potassium on Microbial and Organoleptic Spoilage Patterns in Sliced Vacuum-Packed Pasteurized Pork Loin

1986 ◽  
Vol 49 (12) ◽  
pp. 999-1002 ◽  
Author(s):  
H.-J. S. NIELSEN ◽  
P. ZEUTHEN
Keyword(s):  
Adverse Effect ◽  
Sodium Chloride ◽  
Potassium Chloride ◽  
Aerobic Bacteria ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Brochothrix Thermosphacta ◽  
Sodium Substitution ◽  
Pork Loin ◽  
Sodium And Potassium

Sliced, cured, cooked and smoked pork loin was produced with sodium chloride or a mixture of sodium and potassium chloride, with each preparation of pork loin having the same water activity (0.967–0.968). The pork loins were sliced, vacuum packaged and stored at 2, 5 and 10°C. Microbial spoilage was determined using selective and nonselective media to enumerate total aerobic bacteria, lactics, Brochothrix thermosphacta, gram-negative bacteria and yeasts. Spoilage was also determined using sensory evaluation. Generally, the influence of sodium substitution on microorganisms was minimal. Organoleptic scores were similar for the two preparations of pork loin, hence no adverse effect of sodium substitution was observed.

Diversity of culturable heterotrophic aerobic bacteria in pristine stream bed sediments

1999 ◽  
Vol 45 (10) ◽  
pp. 879-884 ◽  
Author(s):  
L Halda-Alija ◽  
T C Johnston
Keyword(s):  
Fatty Acid ◽  
Restriction Analysis ◽  
Fatty Acid Analysis ◽  
Aerobic Bacteria ◽  
Gram Negative Bacteria ◽  
Bed Sediments ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Genotypic Analysis ◽  
Dna Restriction

More than 900 culturable, heterotrophic aerobic isolates were obtained from the sediments of a forested, pristine stream and analyzed using three classical microbiological tests: API 20E, amplified ribosomal DNA restriction analysis (ARDRA), and fatty acid analysis. Gram-negative bacteria comprised most of the heterotrophic aerobic isolates (66.7%), similar to other oligotrophic environments. The isolates were assigned to the genus level as Pseudomonas, Flavobacterium, Micrococcus, Bacillus, Chromobacterium, Acinetobacter, Alcaligenes, Aeromonas, Methylobacterium, Enterobacter, Corynebacterium, and Sporolactobacillus. Genotypic analysis by ARDRA facilitated the comparison among strains within Pseudomonas, Bacillus, and Enterobacter groups. Temperature and predation may influence the survival of bacteria during seasons, as shown previously by others. Our results showed that the number of heterotrophic aerobic bacteria, especially Enterobacter, Alcaligenes, and Aeromonas, and Gram-positive bacteria, decreased in winter compared to summer conditions.Key words: stream, heterotrophic aerobic bacteria, ARDRA.

THE COLIFORM BACTERIA OF STRAWBERRIES*

1954 ◽  
Vol 17 (12) ◽  
pp. 362-365
Author(s):  
J. Orvin Mundt ◽  
G. A. Shuey ◽  
Ivan C. McGarty
Keyword(s):  
Brilliant Green ◽  
Coliform Bacteria ◽  
Animal Origin ◽  
Gram Negative Bacteria ◽  
Gram Negative

The positive presumptive tests obtained on the examination of strawberries undergoing processing for freezing are generally caused by Gram negative bacteria indigenous to plants and to the soil. The bacteria produce gas at the expense of the sugars naturally present in the berries. Washing the berries in chlorinated water or in water containing detergents does not eliminate the microorganisms. The incidence of coliform bacteria appears to be associated with dirty fruit, and soft and unsound fruit. A greater number of positive presumptive tests are obtained with lauryl tryptose broth than with brilliant green bile 2 percent broth. The former medium is more efficient in detecting the organisms of human and of animal origin.

scholarly journals IDENTIFIKASI BAKTERI AEROB GRAM NEGATIF DAN GRAM POSITIF MENGGUNAKAN METODE KONVENSIONAL DAN OTOMATIK

2018 ◽  
Vol 19 (2) ◽  
pp. 105
Author(s):  
Patricia M. Tauran ◽  
Irda Handayani ◽  
Nurhayana Sennang
Keyword(s):  
Aerobic Bacteria ◽  
Gram Negative Bacteria ◽  
Bacterial Taxonomy ◽  
Biochemical Tests ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Automated Method ◽  
Automatic Methods ◽  
Automated Methods

Choosing the method of bacteria identification is crucial to obtain accurate and quick results. This study will analyze the identificationresults of Gram negative and Gram positive from aerobic bacteria by examination using conventional and automatic methods at Dr.Wahidin Sudirohusodo Hospital Laboratory. A total of 85 samples consisting of 66 Gram negative bacteria and 19 Gram positive bacteriawere identified using conventional and automated methods. In this study, there was some correspondent identification result betweenthe conventional as well as the automated methods, namely 31.5% for Gram negative bacteria and 30.8% for Gram positive bacteria.However, the non-correspondent identification result between conventional and automated methods was found greater, namely, 68.5%for Gram negative bacteria and 69.2% for Gram positive bacteria. The non-correspondent identification result was due to the developmentof bacterial taxonomy and the differences of numbers and types of the biochemical tests between conventional and automatic methods.Bacteria identification using automated method is more accurate and faster than the conventional method, so it is recommended usingthis particularly for the laboratory and educational referral center.

scholarly journals Bakteri Aerob Penyebab Infeksi Nosokomial di Ruang Bedah RSU Abepura, Kota Jayapura, Papua

2018 ◽  
Vol 4 (2) ◽  
pp. 63-68
Author(s):  
Daniel Lantang ◽  
Desi Paiman
Keyword(s):  
Listeria Monocytogenes ◽  
Nosocomial Infection ◽  
Brain Heart Infusion ◽  
Aerobic Bacteria ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Agar Media ◽  
Identification Of Bacteria

The research on identification of aerobic bacteria causing nosocomial infection in the surgery room of General Hospital (RSU) Abepura, Jayapura had been done from August–December 2008. Samples were taken from room air (30 minutes) using blood agar media and from certain tools at some points using brain heart infusion media (BHI). The colony appearance was observed after 24 hours of incubation period of bacterial growth. Further analysis to identify the aerobic bacteria causing nosocomial infections was done by several tests in Jayapura Health Laboratory. The result showed that there were 15 aerobic bacteria consisted of: the coccid gram–positive bacteria: Staphylococus epidermidis, S. aureus, S. saprophyticus, Streptcoccus sp; the rod gram-positive bacteria: Listeria monocytogenes, Diptheroid sp, Lactobacillus sp and gram-negative bacteria: Providence rettgeri, Pseudomonas puttrefaciens, Klebsiela ozaena, P. malthophyla, Morganela morganii, Serattia sp, K. oxytoca, and K. pneumonia. Key words: Aerobic bacteria, identification of bacteria, nosocomial infection, surgical room, Jayapura.

Lactate production by aerobic bacteria grown in cerebrospinal fluid.

1984 ◽  
Vol 30 (11) ◽  
pp. 1875-1876 ◽  
Author(s):  
F L Kiechle ◽  
M A Kamela ◽  
R W Starnes
Keyword(s):  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
Lactate Concentration ◽  
Lactate Production ◽  
Aerobic Bacteria ◽  
Gram Negative Bacteria ◽  
High Concentration ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Abstract The source of the abnormally high concentration of lactate in the cerebrospinal fluid in patients with bacterial meningitis is not known. It may represent a bacterial metabolite. To determine whether cerebrospinal fluid possesses appropriate substrates to support the growth of pathogenic aerobic bacteria, three Gram-positive and nine Gram-negative bacteria were separately inoculated in pooled normal cerebrospinal fluid. After incubation for 24 h, all Gram-positive bacteria increased lactate, eight Gram-negative bacteria decreased lactate, and one Gram-negative bacteria failed to significantly change the lactate concentration. We conclude that lactate produced in cerebrospinal fluid in patients with aerobic bacterial meningitis is not necessarily a bacterial metabolite.

scholarly journals Microbiological Quality Assessment of Popular Fresh Date Samples Available in Local Outlets of Dhaka City, Bangladesh

2018 ◽  
Vol 2018 ◽  
pp. 1-4
Author(s):  
Rausan Zamir ◽  
A. B. M. Nazmul Islam ◽  
Anisur Rahman ◽  
Sunzid Ahmed ◽  
M. Omar Faruque
Keyword(s):  
Food Poisoning ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Aerobic Bacteria ◽  
Dhaka City ◽  
Hygiene Practices ◽  
Yeasts And Molds ◽  
Potential Human Health ◽  
Date Fruits

It is evident that date fruits provide a wide variety of essential nutrients which impart potential human health benefits. In Bangladesh, the popularity of date and its consumption surge few-fold during Ramadan among Muslims owing to the profound emotion related to religious belief that breaking of dawn-to-dusk fasting with dates is fulfilling a Sunnah. The present study aimed to explore the microbiological quality of the five most popular fresh date samples (Nagal, Dhapas, Boroi, Morium, and Tunisia) purchased from different locations of Dhaka City, Bangladesh. Among 25 samples tested, 23 were observed to carry 3.30-5.65 Log CFU/gm aerobic bacteria and 3.30-5.36 Log CFU/gm yeasts and molds population. Coliform bacteria and Escherichia coli were not found in any of the samples analyzed in this study. However, except Nagal and Boroi from Mohammadpur and Mirpur, respectively, none of the samples were found safe to consume according to the microbiological grade recommended by Woolworths Quality Assurance Standard (WQAS), 2009, as fresh dates with microbial load can cause food poisoning or even foodborne intoxication. Date samples with less or no processing is responsible for this contamination which can be subsided and eliminated by appropriate handling and hygiene practices during postharvest processing.

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