A Comparison of Six Selective Media for the Enumeration and Isolation of Staphylococci1

1988 ◽  
Vol 51 (9) ◽  
pp. 685-690 ◽  
Author(s):  
D. G. WHITE ◽  
J. S. MATOS ◽  
R. J. HARMON ◽  
B. E. LANGLOIS
Keyword(s):  
Blood Agar ◽  
Control Medium ◽  
Bovine Blood ◽  
Mannitol Salt Agar ◽  
Selective Media ◽  
Specific Medium

The efficacy of Baird-Parker agar, Baird-Parker agar with bacitracin (0.8 μg/ml), Baird-Parker agar supplemented with acriflavin (7 μg/ml), polymyxin (20 units/ml) and sulphonamides (55 μg/ml), KRANEP agar, mannitol salt agar, and Staphylococcus medium 110 agar for the isolation and enumeration of Staphylococcus species was investigated. Bovine blood agar was used as the control medium. Thirty-seven staphylococci strains representing 23 species and 19 non-Staphylococcus species were tested. None of the six selective media supported the growth of all 37 Staphylococcus strains. The number of Staphylococcus species that grew on a specific medium ranged from only the S. aureus strains on Baird-Parker agar supplemented with acriflavin, polymyxin, and sulphonamides, to all but S. warneri BG 647 on Staphylococcus medium 110 agar. Strains of Bacillus, Corynebacterium, and Micrococcus grew on all six selective media.

scholarly journals Influence of local tetracycline on the microbiota of alveolar osteitis in rats

2008 ◽  
Vol 19 (2) ◽  
pp. 119-123 ◽  
Author(s):  
Joseane Maria Dias Bosco ◽  
Sérgio Ricardo de Oliveira ◽  
Álvaro Francisco Bosco ◽  
Christiane Marie Schweitzer ◽  
Elerson Gaetti Jardim Júnior
Keyword(s):  
Aqueous Solution ◽  
Wistar Rats ◽  
Blood Agar ◽  
Selective Media ◽  
Sterile Saline ◽  
Alveolar Osteitis ◽  
Microbial Samples

The aim of the present study was to evaluate the effects of local tetracycline on the occurrence of alveolar osteitis in rats, and on the microbiota associated to this infection. Forty Wistar rats were randomly assigned to 4 groups (n=10): I - the rats had the maxillary right incisor extracted and the alveolar wound did not receive any treatment; II - adrenaline and Ringer-PRAS were introduced into the alveolar wound; III - the alveolar wound was irrigated with sterile saline; and IV - the alveolar wound was irrigated with an aqueous solution of tetracycline. Microbial samples from the alveolar wounds were collected 2 days after surgery and inoculated on blood agar (with and without 8 µg/mL of tetracycline) and other selective media, and were incubated in either aerobiosis or anaerobiosis at 37ºC, for 2 to 14 days. It was verified that tetracycline reduced the occurrence of alveolar osteitis in the rats and caused significant changes in the microbiota of the surgical sites, decreasing the number of anaerobes and increasing the participation of tetracycline-resistant and multi-resistant microorganisms.

scholarly journals Case Study: Misdiagnosis of Nonhemolytic Staphylococcus aureus Isolates from Cases of Bovine Mastitis as Coagulase-Negative Staphylococci

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 252
Author(s):  
Valerie E. Ryman ◽  
Felicia M. Kautz ◽  
Steve C. Nickerson
Keyword(s):  
Staphylococcus Aureus ◽  
Bovine Mastitis ◽  
Dairy Farm ◽  
Blood Agar ◽  
Coagulase Negative Staphylococci ◽  
Mannitol Salt Agar ◽  
Lactating Cows ◽  
Presumptive Identification ◽  
Microbiological Techniques

Staphylococcus aureus is one of the most concerning mastitis-causing pathogens in dairy cattle. Using basic microbiological techniques, S. aureus is typically identified by colony characteristics and hemolysis on blood agar where isolates without hemolysis are typically considered to be coagulase-negative staphylococci (CNS) isolates. Herein, we present a decade-long case study where suspected S. aureus isolates from one Georgia dairy farm were further tested to confirm presumptive identification. Presumptive identification of bacterial growth from 222 mammary secretions from bred Holstein heifers and lactating cows was conducted at the time of collection. Presumptive identification of S. aureus on blood agar was based on observation of colony morphology, color, and presence or absence of a broad zone of incomplete hemolysis and a smaller zone of complete hemolysis at 48 h. Those without hemolysis were presumptively characterized as CNS. All isolates were further plated on mannitol salt agar and a coagulase test was performed. A positive for both of these tests together was deemed to be S. aureus. A selection of isolates was tested using API® Staph to biochemically confirm S. aureus identification. Data showed that 63.96% of isolates presumed to be CNS isolates were identified as S. aureus, 9.46% of isolates presumed to be CNS isolates were identified as coagulase-positive staphylococci (CPS) species (but not S. aureus), and 26.58% of samples that were presumed to be CNS isolates were identified correctly.

scholarly journals Identifikasi Staphylococcus aureus pada Daging Ayam yang Dijual di Pasar Besar Kota Palangka Raya

2019 ◽  
Vol 1 (1) ◽  
pp. 13-16
Author(s):  
Rahmawati Rahmawati ◽  
Eva Apriliana ◽  
Agus Agus
Keyword(s):  
Staphylococcus Aureus ◽  
High Temperatures ◽  
Staphylococcus Epidermidis ◽  
Food Poisoning ◽  
Chicken Meat ◽  
High Protein ◽  
Mannitol Salt Agar ◽  
Selective Media ◽  
Contaminated Food

Staphylococcus aureus can be a cause of food poisoning in humans. This type of bacteria can produce enterotoxins that cause contaminated food and poisoning in humans. The toxin produced is resistant in high temperatures, although the bacteria die by heating but the resulting toxin will not be damaged and can still change despite freezing or freezing. Staphylococcus aureus is commonly found in high-protein foods such as egg and chicken products. The purpose of this study was to investigate the contamination of Staphylococcus aureus bacteria in chicken meat sold in Pasar Besar Kota Palangka Raya in 2018. The examination using MSA media (Mannitol Salt Agar) because MSA media is different selective media to differentiate Staphylococcus aureus with Staphylococcus epidermidis. The results of analysis of chicken meat in Pasar Besar Kota Palangka Raya 20% positive contaminated Staphylococcus aureus bacteria in the first-day sample. In the second day, the sample found 13.3% positive contaminated Staphylococcus aureus bacteria.

Recovery of Streptococci from a Variety of Foods: A Comparison of Several Media1

1975 ◽  
Vol 38 (6) ◽  
pp. 323-326 ◽  
Author(s):  
J. L. OBLINGER
Keyword(s):  
Food Samples ◽  
Blood Agar ◽  
Selective Media ◽  
Solid Media ◽  
Ethyl Violet

Nine liquid and solid media used to isolate and/or enumerate streptococci were evaluated using 30 food samples. Streptococci were recovered from all of the samples surveyed. Azide Blood agar gave the highest recovery of streptococci and also allowed the observation of hemolytic reactions. Streptosel, KF streptococcus, and m-Enterococcus agars were also used, with the latter giving the lowest recovery among the agars. Azide Dextrose, KF, and Streptosel broths gave similar recoveries from most samples. Confirmation of Azide Dextrose isolates in Ethyl Violet Azide broth lowered streptococcal estimates by several log cycles. When isolates from both agar and broth cultures were subjected to further testing. Lactobacillus and Staphylococcus were often encountered, thus indicating the need to confirm representative numbers of colonies even if they are from selective media.

Isolation and Identification of Staphylococcus Aureus from Dairy Farms in Bishoftu Town, Ethiopia

2021 ◽  
Author(s):  
Sara Amanuel Bude ◽  
Abdi Kidane Mengesha
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Farms ◽  
Nutrient Agar ◽  
Blood Agar ◽  
Swab Sample ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Mannitol Salt Agar ◽  
Milk Samples ◽  
Golden Yellow

Abstract A cross-sectional study was conducted from February, 2020 to March, 2020 in selected dairy farms in Bishoftu Town to isolate and identify Staphylococcus aureus from milk samples, swab (teat swab, tank swab and bucket swab) samples. The samples were transported to microbiology laboratory and, isolation and identification of an organism was based on morphological, cultural and biochemical characteristics. Accordingly a total of 120 samples of them 58 milk sample, 58 polled teat swab sample, 2 polled tank swab sample and 2 polled bucket swab samples. Those milk samples and swab sample were collected and cultured parallel on both Blood agar and nutrient agar. Out of total samples 68/120 were well grown on both Medias and sub cultured on nutrient agar for farther identification using Primary and secondary biochemical tests. Accordingly, 68 milk samples were shown typical large, round, golden yellow colonies with hemolysis when grown on blood agar, round shaped grape like clusters are seen under microscope after stained with grams stain, and catalase positive Staphylococcus aureus with bubble formation is observed and typical yellow pigmentation on mannitol salt agar which is selective media for genus Staphylococcus. Finally, 68(56.67%) Staphylococcus aureus were identified with coagulase test with the clumping result observed. Hence, implementing hygiene conditions, creation of awareness on control and prevention of subclinical mastitis in dairy farms and conducting drug sensitivity test for Staphylococcus aureus is recommended.

Comparison of selective media for the enumeration of sublethally heated food-poisoning strains of Staphylococcus aureus

1974 ◽  
Vol 20 (7) ◽  
pp. 1072-1075 ◽  
Author(s):  
D. L. Collins-Thompson ◽  
A. Hurst ◽  
B. Aris
Keyword(s):  
Staphylococcus Aureus ◽  
Phosphate Buffer ◽  
Food Poisoning ◽  
Potassium Phosphate ◽  
Egg Yolk ◽  
Potassium Phosphate Buffer ◽  
Mannitol Salt Agar ◽  
Selective Media

Staphylococcus aureus strains (19 food-poisoning strains) were heated at 52C for 15 min in 100 mM potassium phosphate buffer. The sublethally heated organisms were enumerated on seven selective media commonly used for quantitating S. aureus. Compared to trypticase soy agar, Baird-Parker agar was the only medium which was satisfactory with all cultures tested. The remaining selective media gave unsatisfactory recoveries with one or more of the strains tested and are listed in the following order of decreasing acceptability: tellurite glycine agar, egg yolk azide agar, phenolphthalein phosphate agar with polymyxin, mannitol salt agar, Vogel Johnson agar.

scholarly journals Dentistry and Intensive Care Unit: A Brief Report

2021 ◽  
Author(s):  
Lisiane Cristina Bannwart ◽  
Clóvis Lamartine de Moraes Melo Neto ◽  
Daniela Micheline dos Santos ◽  
André Luiz de Melo Moreno ◽  
Aldiéris Alves Pesqueira ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Culture Media ◽  
Blood Agar ◽  
Macconkey Agar ◽  
Candida Spp ◽  
Removable Partial Denture ◽  
Mannitol Salt Agar ◽  
Sabouraud Agar ◽  
Shigella Spp

Abstract Objective The aim of this study is to verify whether removable dentures of patients admitted to an intensive care unit (ICU) are niches of microorganisms that can cause pathologies (Staphylococcus aureus, Candida spp., and enterobacteria). Materials and Methods Fifteen patients who were denture wearers (removable partial denture and complete denture) were included in this study. Patients must wear their dentures daily, and these dentures must have acrylic parts. Microbial biofilm was collected from the acrylic part of one denture of each patient. Then, the biofilm was seeded on different culture media: Sabouraud agar, blood agar, MacConkey agar, and mannitol salt agar. In this study, biochemical evaluations of microorganisms were performed. Statistical analysis The percentage of dentures with the microorganism identified by each culture medium was calculated. Results In total, 100% of the dentures were positive for Staphylococcus spp. (blood agar) and Candida spp. (Sabouraud agar); 33.3% of the dentures were positive for S. aureus (Mannitol salt agar); and 13.3% of the dentures were positive for Shigella spp. (MacConkey agar). Conclusion Removable dentures of patients (removable partial dentures and complete dentures) admitted to an ICU are niches of microorganisms that can cause pathologies.

scholarly journals Growth of Streptococcus mutans on various selective media

1976 ◽  
Vol 4 (1) ◽  
pp. 95-98
Author(s):  
C G Emilson ◽  
D Bratthall
Keyword(s):  
Streptococcus Mutans ◽  
Growth Phase ◽  
Blood Agar ◽  
Similar Data ◽  
Logarithmic Growth Phase ◽  
Batch Cultures ◽  
Selective Media ◽  
Serotype A ◽  
Colony Forming Units ◽  
Logarithmic Growth

The ability of Streptococcus mutans to grow on mitis-salivarius (MS) agar, MC agar, mitis-sucrose-bacitracin (MSB), BCY agar, and MM10 sucrose agar was studied. Batch cultures of S. mutans serotype a demonstrated no growth on MSB agar. Certain serotype d and g strains did not grow on MC agar. The yield for most strains of other serotypes on these selective media was lower compared with that on MS agar. The number of total colony-forming units on BCY and MM10 sucrose agar was similar to the blood agar results. Similar data were obtained when fermenter-grown strains, harvested in the middle or the end of the logarithmic growth phase, were used for inoculation of the various media. Enumeration of S. mutans from plaque samples plated on MC and MSB agar yielded about 75% of the counts obtained on MS or the nonselective medium. When the proportions of S. mutans were expressed as a percentage of the total cultivable flora, the selective media (MC and MSB agar) showed approximately 10% lower values than the MS, BCY, and MM10 sucrose agar.

scholarly journals COMPARATIVE EVALUATION OF SIX SELECTIVE MEDIA FOR THE DETECTION AND ENUMERATION OF STAPHYLOCOCCUS AUREUS IN FOODS

1974 ◽  
Vol 25 (4) ◽  
pp. 10
Author(s):  
Β. ΑΛΜΠΑΛΑΣ
Keyword(s):  
Staphylococcus Aureus ◽  
Comparative Evaluation ◽  
Egg Yolk ◽  
Mannitol Salt Agar ◽  
Selective Media ◽  
Pure Cultures ◽  
The Media ◽  
Coagulase Positive Staphylococci ◽  
The Ideal

Six selective media for the detection and enumeration of coagulase-positive staphylococci werl ctudied evaluated for their efficiency in the recovery and easy recognition of five strains of Staphylococcus aureus. They were Mannitol Salt agar (MS', Tellurite Polymyxine Egg Yolk agar (TPEY),Egg Yolk Azide Agar (EYAA), Egg yolk Tellurite Glycine Pyruvate Agar (ETGPA). Milk Salt Agar (MSA), in combination with Salt Egg Yolk Agar (SEYA), and Vogel-Johnson agar (VJ), after enrichement in Trypticase Soy Broth 10V» NaCl.The recovery of the used strains was evaluated in pure cultures and after inoculation in various foods. None of the tested media was proved to be the ideal one. The media TPEY, ETGRA. and EYAA were considered as the nost appropriate for the detection of S. aureus in foods. The selective ability of eash medium is greatly depended on the strain and the food involved The simultaneoue use of two selective media is suggested if that is possible.

Reliability of Selective Media for Recovery of Staphylococci from Cheese

1977 ◽  
Vol 40 (1) ◽  
pp. 11-16 ◽  
Author(s):  
M. E. STILES
Keyword(s):  
Staphylococcus Aureus ◽  
Significant Interaction ◽  
Egg Yolk ◽  
Selective Medium ◽  
Selective Agent ◽  
Mannitol Salt Agar ◽  
Selective Media ◽  
Cheese Ripening ◽  
Different Strains ◽  
Blood Agar Base

The reliability of 12 selective media for recovery of four different strains of Staphylococcus aureus inoculated at manufacture into three cheese types was determined. Selective medium and time of ripening had a highly significant effect (p < 0.001) on reliability of the staphylococcal count. In addition, highly significant interaction effects were observed. The most reliable medium in the overall analysis was mannitol salt agar. However, this medium was not equally reliable at all times during ripening, and use of both mannitol salt agar and Staphylococcus medium no. 110 is recommended. The tellurite- and azide-based selective media were generally unsatisfactory, however tellurite glycine agar, Vogel Johnson (VJ) agar, and azide blood agar base were totally unreliable. In general, the salt-based selective media were most reliable. This applied also to the egg yolk media that use salt as the selective agent. Salt egg yolk agar and Colbeck's egg yolk medium generally gave higher recoveries of S. aureus than did Baird-Parker medium, Crisley et al. tellurite polymyxin egg yolk agar, and Hopton egg yolk azide agar, except in the unripened cheeses. The debilitating effect of cheese ripening on the staphylococcal cells was not eliminated by the egg yolk tellurite and azide media.

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