Reliability of Selective Media for Recovery of Staphylococci from Cheese

1977 ◽  
Vol 40 (1) ◽  
pp. 11-16 ◽  
Author(s):  
M. E. STILES
Keyword(s):  
Staphylococcus Aureus ◽  
Significant Interaction ◽  
Egg Yolk ◽  
Selective Medium ◽  
Selective Agent ◽  
Mannitol Salt Agar ◽  
Selective Media ◽  
Cheese Ripening ◽  
Different Strains ◽  
Blood Agar Base

The reliability of 12 selective media for recovery of four different strains of Staphylococcus aureus inoculated at manufacture into three cheese types was determined. Selective medium and time of ripening had a highly significant effect (p < 0.001) on reliability of the staphylococcal count. In addition, highly significant interaction effects were observed. The most reliable medium in the overall analysis was mannitol salt agar. However, this medium was not equally reliable at all times during ripening, and use of both mannitol salt agar and Staphylococcus medium no. 110 is recommended. The tellurite- and azide-based selective media were generally unsatisfactory, however tellurite glycine agar, Vogel Johnson (VJ) agar, and azide blood agar base were totally unreliable. In general, the salt-based selective media were most reliable. This applied also to the egg yolk media that use salt as the selective agent. Salt egg yolk agar and Colbeck's egg yolk medium generally gave higher recoveries of S. aureus than did Baird-Parker medium, Crisley et al. tellurite polymyxin egg yolk agar, and Hopton egg yolk azide agar, except in the unripened cheeses. The debilitating effect of cheese ripening on the staphylococcal cells was not eliminated by the egg yolk tellurite and azide media.

Comparison of selective media for the enumeration of sublethally heated food-poisoning strains of Staphylococcus aureus

1974 ◽  
Vol 20 (7) ◽  
pp. 1072-1075 ◽  
Author(s):  
D. L. Collins-Thompson ◽  
A. Hurst ◽  
B. Aris
Keyword(s):  
Staphylococcus Aureus ◽  
Phosphate Buffer ◽  
Food Poisoning ◽  
Potassium Phosphate ◽  
Egg Yolk ◽  
Potassium Phosphate Buffer ◽  
Mannitol Salt Agar ◽  
Selective Media

Staphylococcus aureus strains (19 food-poisoning strains) were heated at 52C for 15 min in 100 mM potassium phosphate buffer. The sublethally heated organisms were enumerated on seven selective media commonly used for quantitating S. aureus. Compared to trypticase soy agar, Baird-Parker agar was the only medium which was satisfactory with all cultures tested. The remaining selective media gave unsatisfactory recoveries with one or more of the strains tested and are listed in the following order of decreasing acceptability: tellurite glycine agar, egg yolk azide agar, phenolphthalein phosphate agar with polymyxin, mannitol salt agar, Vogel Johnson agar.

scholarly journals COMPARATIVE EVALUATION OF SIX SELECTIVE MEDIA FOR THE DETECTION AND ENUMERATION OF STAPHYLOCOCCUS AUREUS IN FOODS

1974 ◽  
Vol 25 (4) ◽  
pp. 10
Author(s):  
Β. ΑΛΜΠΑΛΑΣ
Keyword(s):  
Staphylococcus Aureus ◽  
Comparative Evaluation ◽  
Egg Yolk ◽  
Mannitol Salt Agar ◽  
Selective Media ◽  
Pure Cultures ◽  
The Media ◽  
Coagulase Positive Staphylococci ◽  
The Ideal

Six selective media for the detection and enumeration of coagulase-positive staphylococci werl ctudied evaluated for their efficiency in the recovery and easy recognition of five strains of Staphylococcus aureus. They were Mannitol Salt agar (MS', Tellurite Polymyxine Egg Yolk agar (TPEY),Egg Yolk Azide Agar (EYAA), Egg yolk Tellurite Glycine Pyruvate Agar (ETGPA). Milk Salt Agar (MSA), in combination with Salt Egg Yolk Agar (SEYA), and Vogel-Johnson agar (VJ), after enrichement in Trypticase Soy Broth 10V» NaCl.The recovery of the used strains was evaluated in pure cultures and after inoculation in various foods. None of the tested media was proved to be the ideal one. The media TPEY, ETGRA. and EYAA were considered as the nost appropriate for the detection of S. aureus in foods. The selective ability of eash medium is greatly depended on the strain and the food involved The simultaneoue use of two selective media is suggested if that is possible.

scholarly journals Identifikasi Staphylococcus aureus pada Daging Ayam yang Dijual di Pasar Besar Kota Palangka Raya

2019 ◽  
Vol 1 (1) ◽  
pp. 13-16
Author(s):  
Rahmawati Rahmawati ◽  
Eva Apriliana ◽  
Agus Agus
Keyword(s):  
Staphylococcus Aureus ◽  
High Temperatures ◽  
Staphylococcus Epidermidis ◽  
Food Poisoning ◽  
Chicken Meat ◽  
High Protein ◽  
Mannitol Salt Agar ◽  
Selective Media ◽  
Contaminated Food

Staphylococcus aureus can be a cause of food poisoning in humans. This type of bacteria can produce enterotoxins that cause contaminated food and poisoning in humans. The toxin produced is resistant in high temperatures, although the bacteria die by heating but the resulting toxin will not be damaged and can still change despite freezing or freezing. Staphylococcus aureus is commonly found in high-protein foods such as egg and chicken products. The purpose of this study was to investigate the contamination of Staphylococcus aureus bacteria in chicken meat sold in Pasar Besar Kota Palangka Raya in 2018. The examination using MSA media (Mannitol Salt Agar) because MSA media is different selective media to differentiate Staphylococcus aureus with Staphylococcus epidermidis. The results of analysis of chicken meat in Pasar Besar Kota Palangka Raya 20% positive contaminated Staphylococcus aureus bacteria in the first-day sample. In the second day, the sample found 13.3% positive contaminated Staphylococcus aureus bacteria.

scholarly journals Recovery and Enumeration of Staphylococcus aureus by the Selective Agar

Fine Focus ◽  
2016 ◽  
Vol 2 (1) ◽  
pp. 51-59
Author(s):  
Jill Bange ◽  
Emily Brumfield ◽  
Alysha L. Ellison
Keyword(s):  
Staphylococcus Aureus ◽  
Food Processing ◽  
Recovery Period ◽  
False Negative ◽  
Selective Medium ◽  
Bacterial Cells ◽  
Human Immune System ◽  
Selective Media ◽  
Selective Agar ◽  
Overlay Method

Staphylococcus aureus isan example of a commensal bacterium responsible for emesis, acute diarrheal syndrome, and sepsis. S. aureus often must be isolated from patient samples in a clinical setting or from food samples during food processing in an industrial setting, although these bacterial cells may be injured by the human immune system or by food processing measures. Therefore, injured cells may not be fully recovered on media selective for S. aureus and enumeration (e.g., CFU/mL) may not reflect the true concentration of the original sample. The objective of this study was to determine whether the selective agar overlay method of recovery is more sensitive, selective, and time-effective for enumeration of artificially injured S. aureus cultures when compared to more traditional techniques. The selective agar overlay method involves pour plating S. aureus in non-selective medium, allowing the sample to incubate for a four hour recovery period, and then overlaying selective medium over the non-selective medium. Artificial injury of S. aureus cells was accomplished by treatment with carvacrol, an extract from oil of oregano. Our results indicated that carvacrol-injured S. aureus cells were recovered by the selective agar overlay at the same concentration as recovery on non-selective media, and at a significantly higher concentration than recovery on selective media. This method allows for more rapid and accurate diagnoses, and may be more cost-effective due to the reduction or elimination of false negative results.

ICMSF methods studies. X. An international comparative study of four media for the enumeration of Staphylococcus aureus in foods

1978 ◽  
Vol 24 (3) ◽  
pp. 274-281 ◽  
Author(s):  
M. K. Rayman ◽  
J. J. Devoyod ◽  
U. Purvis ◽  
D. Kusch ◽  
J. Lanier ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Comparative Study ◽  
Egg Yolk ◽  
Nuclease Activity ◽  
Selective Media ◽  
International Comparative Study ◽  
Significant Difference ◽  
The Media ◽  
International Comparative

An international comparative study, undertaken by six laboratories to assess the performance of four selective media commonly used for the enumeration of Staphylococcus aureus in foods, revealed that Baird-Parker agar performed most satisfactorily. There was no significant difference among milk salt, tellurite polymyxin egg yolk, and kalium rhodanid – actidione – natriumazid – eigelb – pyruvat (KRANEP) agars. The type of food examined appeared to influence the performance of the media, but no specific patterns could be determined. Cultures yielding 3+ and 4+ coagulase reactions are most likely to possess thermostable nuclease activity, and are therefore most likely to be S. aureus.

Selective Efficacy of Culture Media Recommended for Isolation and Enumeration of Fusarium spp.

2004 ◽  
Vol 67 (1) ◽  
pp. 207-211 ◽  
Author(s):  
M. R. BRAGULAT ◽  
E. MARTÍNEZ ◽  
G. CASTELLÁ ◽  
F. J. CABAÑES
Keyword(s):  
Malachite Green ◽  
Culture Media ◽  
Fungal Species ◽  
Selective Medium ◽  
The Other ◽  
Fusarium Spp ◽  
Selective Media ◽  
Different Strains

Selective culture media, such as Nash and Snyder medium (NS), dichloran-chloramphenicol peptone agar (DCPA), modified Czapek-Dox agar (MCz), Czapek Dox iprodione dichloran agar (CZID), potato dextrose iprodione dichloran agar (PDID), or malachite green agar (MGA 2.5), have been developed for isolating and enumerating Fusarium spp. from natural samples. However, some of these culture media are not very selective because they allow the growth of many other fungal species. In this study, a comparison of the selective efficacy of these culture media, using different strains of Fusarium spp. (F. anthophilum, F. culmorum, F. dlamini, F. graminearum, F. napiforme, F. nygamai, F. oxysporum, F. proliferatum, F. semitectum, F. solani, F. subglutinans, and F. verticillioides) and natural samples has been carried out. Among the six recommended selective culture media assayed, no statistical differences were detected in colony counts of the Fusarium spp. strains tested, although the colony diameters in MGA 2.5 were significantly lower than in NS, MCz, DCPA, CZID, and PDID media. With natural samples, MGA 2.5 performs as a potent selective medium for Fusarium spp., whereas the other recommended selective media allow the growth of many other different fungal species including Zygomycetes and yeasts.

The reliability of selective media for the enumeration of unheated and heated staphylococci

1974 ◽  
Vol 20 (12) ◽  
pp. 1735-1744 ◽  
Author(s):  
M. E. Stiles ◽  
P. C. Clark
Keyword(s):  
Heat Treatment ◽  
Staphylococcus Aureus ◽  
Relative Efficiency ◽  
Protective Action ◽  
Egg Yolk ◽  
Protective Agent ◽  
Selective Media ◽  
Heat Injury

The relative efficiency of 15 selective media for enumerating unheated and sublethally heated strains of Staphylococcus aureus was tested against tryptic soy agar (Difco). Baird-Parker's egg yolk tellurite glycine pyruvate agar was found to be the medium of choice for both unheated and sublethally heated cells. Tellurite polymyxin egg yolk agar and egg yolk azide agar also gave favorable results. For unheated cells, Vogel-Johnson agar and tellurite glycine agar gave satisfactory results, but after heat treatment, even after a 10-h enrichment in tryptic soy broth for resuscitation of the heat injury, these media gave unreliable counts. Egg yolk acted not only as a diagnostic, but also as a protective agent in selective media for staphylococci. The protective action of egg yolk was more effective in azide- and tellurite-containing selective media than in salt-containing selective media.

scholarly journals Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with Mastitis

2003 ◽  
Vol 27 (1) ◽  
pp. 174-179
Author(s):  
Safana, A.S. Al-Taan
Keyword(s):  
Staphylococcus Aureus ◽  
Raw Milk ◽  
Selective Medium ◽  
Isolation And Identification ◽  
Mannitol Salt Agar ◽  
Staphylococcal Species ◽  
Staph Aureus ◽  
Milk Samples ◽  
Positive Results ◽  
Prevalent Pathogen

Twenty-six isolates of staphylococci were recovered from of 100 raw milk samples collected from one hundred cows infected with mastitis. For the isolation of bacteria, Mannitol salt agar was used as a selective medium. Staphylococcal species were identified by API-STAPH system. The identified species were: - Staphy aureus (13), Staph. epidermidis(9), Staph. haemolyticus(2), Staph. lugdunensis()), and Staph. hominis). Staphylococcus aureus was the most prevalent pathogen among other Staphyloccocal species isolated in this study. Staph. aureus isolates revealed positive results for the defection of capsule structure and for production of some essential enzymes such as coagulase, phosphatase, DNA ase and haemolysin which are highly associated with the virulance of bacteria. Staph. aureus isolates were tested for their sensitivity to the antibiotics and the results were: 13(100%) isolates were sensitive to Gentamycin and Tetracyclene, 11 (84.6%) isolates were sensitive to Oxacillin, Penicillin, Ampicillin, Erythromycin and Cephalexin, where as only 2 (15.4%) isolates were Methicillin Resistant Staph. aureus (MRSA) which showed multi -resistant  towards many antibiotics used in this work.

Staphylococcus aureus Nasal Carriage in Medical Students of Universiti Teknologi MARA

2017 ◽  
Vol 2 (1) ◽  
pp. 29 ◽  
Author(s):  
Zaini Mohd Zain ◽  
Muhammad Fikri Johari ◽  
Nurul Shahirah Mohd Husin ◽  
Nurul Syamimi Rozman ◽  
Athirah Ab Rashid ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Medical Students ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Nasal Carriage ◽  
Tissue Necrosis ◽  
Mannitol Salt Agar ◽  
Microbiological Techniques ◽  
Polymerase Chain ◽  
Low Prevalence ◽  
Panton Valentine Leukocidin

Introduction: To determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) nasal carriage and detection of S. aureus leukotoxins among medical students of Universiti Teknologi MARA. Methods: Both sides of the anterior nares of 136 volunteers, comprising 68 preclinical and 68 clinical medical students, were swabbed and immediately cultured onto mannitol salt agar for growth of S. aureus. Standard microbiological techniques were conducted to identify and confirm the S. aureus colonies and susceptibility test against oxacillin were conducted by using Kirby-Bauer method to determine their resistance to methicillin. Polymerase chain reaction was performed for detection of leukotoxins, i.e., Panton-Valentine Leukocidin (PVL) and -haemolysin genes. Results: Nineteen students (14%) consisting of 10 preclinical (14.7%) and 9 clinical (13.2%) were nasal carriers of S. aureus. However, none of the S. aureus isolates were MRSA. No PVL gene was detected but eight of them were positive for -haemolysin gene. Conclusion: There were no MRSA nasal carriers among the medical students, but a low prevalence of S. aureus nasal carriers was detected. These carriers do not pose as high risk because none of the strains of S. aureus possess both the -haemolysin toxin and the PVL toxin that are associated with tissue necrosis.

scholarly journals Detection of Methicillin Resistant Staphylococcus aureus in Dairy Products and Anterior Nares of Dairy Workers

2019 ◽  
Vol 6 ◽  
pp. 59-62
Author(s):  
Ranjana K.C. ◽  
Ganga Timilsina ◽  
Anjana Singh ◽  
Supriya Sharma
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Susceptibility ◽  
Dairy Products ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Dairy Product ◽  
Control Measures ◽  
Diffusion Method ◽  
Mannitol Salt Agar ◽  
Methicillin Resistant ◽  
Dairy Workers

Objectives: To isolate methicillin resistant Staphylococcus aureus (MRSA) from anterior nares of dairy workers and dairy products and assess the antibiotic susceptibility pattern of the isolates. Methods: Swab samples collected from anterior nares of dairy workers and dairy product (butter) were inoculated into mannitol salt agar and incubated at 37ºC for 24 hours. Identification was done based on colony characteristics, Gram's staining, catalase, oxidase and coagulase test. Antibiotic susceptibility testing was done by modified Kirby Bauer disc diffusion method. MRSA was confirmed by using cefoxitin disc. Results: A total of 109 S. aureus (98 from dairy workers and 11 from butter samples) were isolated. Out of them 32 MRSA were isolated from dairy workers and 4 from butter samples. The association between age group and MRSA was found insignificant (p = 0.115). The association of MRSA between male and female workers was found significant (>0.05). About 86% of the MRSA isolates were susceptible to Gentamicin (86.11%) followed by Ciprofloxacin (77.78%). Conclusion: Detection of MRSA among dairy workers and dairy products warrants proper handling and adequate control measures to prevent transmission of MRSA from dairy industry.

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