Microbiological Flora of Aquacultured Hybrid Striped Bass

1993 ◽  
Vol 56 (12) ◽  
pp. 1054-1060 ◽  
Author(s):  
PATTI C. NEDOLUHA ◽  
DENNIS WESTHOFF
Keyword(s):  
Striped Bass ◽  
Foodborne Pathogens ◽  
Yersinia Pseudotuberculosis ◽  
Wild Fish ◽  
Shigella Dysenteriae ◽  
Hybrid Striped Bass ◽  
Bacillus Spp ◽  
Plate Counts ◽  
Aerobic And Anaerobic

Aquacultured hybrid striped bass were examined for quantity and quality of microflora. Plate counts were performed at three temperatures (7, 22, and 35°C) under aerobic and anaerobic conditions. Bacterial loads on the skin, gills, and intestines were similar to those reported for wild fish. Plate counts performed at 22°C yielded the highest counts and the greatest variety of species. The predominant groups of bacteria isolated were Aeromonas spp. (27%), coryneforms (14%), Pseudomonas spp. (12%), Flavobacterium/Cytophaga/Sphingobacterium group (8%), Plesiomonas shigelloides (7%), Bacillus spp. (7%), and Enterobacteriaceae (6%). Human foodborne pathogens Listeria monocytogenes, Staphylococcus aureus, Shigella dysenteriae, Vibrio spp., and Yersinia pseudotuberculosis were also isolated. There were no indications, however, that pond-raised fish present a higher risk of foodborne illness than do wild fish.

Microbiological Analysis of Striped Bass (Morone saxatilis) Grown in Flow-Through Tanks

1995 ◽  
Vol 58 (12) ◽  
pp. 1363-1368 ◽  
Author(s):  
PATTI C. NEDOLUHA ◽  
DENNIS WESTHOFF
Keyword(s):  
Striped Bass ◽  
Foodborne Pathogens ◽  
Morone Saxatilis ◽  
Microbiological Analysis ◽  
Bacillus Spp ◽  
Plate Counts ◽  
Flow Through ◽  
Aerobic And Anaerobic ◽  
Vibrio Spp

Aquacultured striped bass from indoor flow-through tanks were examined for quantity and quality of microflora. Plate counts were performed at three temperatures (7°C, 22°C, and 35°C) under aerobic and anaerobic conditions. Bacterial loads on the skin, gills, and intestines were similar to those reported for wild striped bass and pond-raised hybrid striped bass. The predominant groups of bacteria isolated were Aeromonas spp. (18%), Flavobacterium/Cytophaga species (15%), Comamonadaceae (15%), Plesiomonas shigelloides (13%), Moraxellaceae (6%), Bacillus spp. (4%), and an unidentified gram-negative rod (9%). Foodborne pathogens Staphylococcus aureus and Vibrio spp. were also isolated.

A Bacteriological Survey of Raw Ground Beef1

1977 ◽  
Vol 40 (11) ◽  
pp. 790-794 ◽  
Author(s):  
JAMES F. FOSTER ◽  
JAMES L. FOWLER ◽  
WARREN C. LADIGES
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Retail Store ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Fecal Streptococci ◽  
Plate Counts ◽  
Aerobic And Anaerobic

The microbiological quality of 150 units of raw ground beef obtained from a local retail store was determined. The range of aerobic plate counts was from 6.9 × 104 to 8.3 × 107/g. By using the most probable number method 96.7% of the 150 units were positive for coliforms, 94.7% for Escherichia coli and 61.3% for Staphylococcus aureus. By the plate methods, 99.3% of the units were positive for fecal streptococci and 56% were positive for Clostridium perfringens. No salmonellae were isolated. Aerobic and anaerobic organisms were isolated and identified. E. coli was the most frequently isolated aerobe followed by organisms in the Klebsiella-Enterobacter group. Among the anaerobic isolates, C. perfringens was the organism most frequently encountered.

scholarly journals Novel Genomic Tools for Specific and Real-Time Detection of Biothreat and Frequently Encountered Foodborne Pathogens

2012 ◽  
Vol 75 (4) ◽  
pp. 660-670 ◽  
Author(s):  
ABDELA WOUBIT ◽  
TESHOME YEHUALAESHET ◽  
TSEGAYE HABTEMARIAM ◽  
TEMESGEN SAMUEL
Keyword(s):  
Real Time ◽  
Foodborne Pathogens ◽  
Yersinia Pseudotuberculosis ◽  
Bacterial Species ◽  
Preliminary Test ◽  
Shigella Dysenteriae ◽  
Whole Genome ◽  
Tularensis Subsp ◽  
Primer Sets

The bacterial genera Escherichia, Salmonella, Shigella, Vibrio, Yersinia, and Francisella include important food safety and biothreat agents. By extensive mining of the whole genome and protein databases of diverse, closely and distantly related bacterial species and strains, we have identified novel genome regions, which we utilized to develop a rapid detection platform for these pathogens. The specific genomic targets we have identified to design the primers in Francisella tularensis subsp. tularensis, F. tularensis subsp. novicida, Shigella dysenteriae, Salmonella enterica serovar Typhimurium, Vibrio cholerae, Yersinia pestis, and Yersinia pseudotuberculosis contained either known genes or putative proteins. Primer sets were designed from the target regions for use in real-time PCR assays to detect specific biothreat pathogens at species or strain levels. The primer sets were first tested by in silico PCR against whole-genome sequences of different species, subspecies, or strains and then by in vitro PCR against genomic DNA preparations from 23 strains representing six biothreat agents (Escherichia coli O157:H7 strain EDL 933, Shigella dysenteriae, S. enterica serovar Typhi, F. tularensis subsp. tularensis, V. cholerae, and Y. pestis) and six foodborne pathogens (Salmonella Typhimurium, Salmonella Saintpaul, Shigella sonnei, F. tularensis subsp. novicida, Vibrio parahaemolyticus, and Y. pseudotuberculosis). Each pathogen was specifically identifiable at the genus and species levels. Sensitivity assays performed with purified DNA showed the lowest detection limit of 128 fg of DNA/μl for F. tularensis subsp. tularensis. A preliminary test to detect Shigella organisms in a milk matrix also enabled the detection of 6 to 60 CFU/ml. These new tools could ultimately be used to develop platforms to simultaneously detect these pathogens.

Microbiological Analysis of Striped Bass (Morone saxatilis) Grown in a Recirculating System†

1997 ◽  
Vol 60 (8) ◽  
pp. 948-953 ◽  
Author(s):  
PATTI C. NEDOLUHA ◽  
DENNIS WESTHOFF
Keyword(s):  
Striped Bass ◽  
Morone Saxatilis ◽  
Microbiological Analysis ◽  
Food Borne Pathogens ◽  
Group I ◽  
Food Borne ◽  
Vibrio Mimicus ◽  
Plate Counts ◽  
Tank Water

The skin, gills, intestines, tank water, and diet of striped bass grown in recirculating tanks were examined for quantity and quality of microflora. Plate counts for the fish and water samples were similar to counts reported in other systems. The bacteria groups represented most frequently in isolates from the fish were Aeromonas (14%), Moraxellaceae (15%), the Flavobacterium-Chryseobacterium-Cytophaga-Sphingobacterium group (11 %), Bacillus (8%), Group I Pseudomonas (6%), the Shewanella-Alteromonas group (6%), and Comamonadaceae (5%). The food-borne pathogens Staphylococcus aureus, motile Aeromonas spp., and Vibrio mimicus were isolated repeatedly. V. parahaemolyticus and V. cholera were each isolated once, and no Listeria, Plesiomonas, Salmonella, or Yersinia enterocolitica were isolated. Close taxonomic relationships were observed among some of the bacteria found on the fish and in the water; bacteria from the skin and gills were more likely to be related to each other and to the water isolates than to the intestinal isolates. Some bacteria in the intestines originated in the diet, but these strains did not significantly affect the overall microflora of the fish and water.

scholarly journals The effect of different fat content fish feed on the production parameters and meat quality of the hybrid striped bass

2013 ◽  
pp. 33-37
Author(s):  
Péter Juhász ◽  
Péter Bársony ◽  
Éva Csorvási ◽  
István Szűcs ◽  
Judit Remenyik ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Striped Bass ◽  
Nutritive Value ◽  
Fat Content ◽  
Fish Feed ◽  
Hybrid Striped Bass ◽  
Omega 3 ◽  
Feed Conversion ◽  
Omega 6

The interspecific ’hybrid striped bass’ is the result of cross breeding. Their offspring have got good chances of growth and tolerate extreme water temperatures and the content of dissolute oxygen more than their parents. Furthermore, they are more resistant to diseases. Therefore, the hybrid is perfectly suitable for intensive fishery production. Our experiment lasted for seven weeks. We analysed the indices of growth and production of the hybrid as the consequence of nurturing them with two feeds of high nutritive value during this period. We examined the content of fat and of fatty acids in the flesh of these fish. There were no considerable differences in terms of weight gain, feed conversion ratio and uneven growth between the two groups, while survival was less likely among the the fish which had consumed more fat. We demonstrated, examining the flesh of the individuals which had consumed feed containing lower fat content, that it was fattier and contained more dry matter than those that had been fed of higher fat content. Besides, the content of omega-3 and omega-6 fatty acids was higher in the fish that had consumed less lipids.

Urban and Community Fisheries Programs: Development, Management, and Evaluation

2008 ◽  
Keyword(s):  
Striped Bass ◽  
Survival Rates ◽  
Total Mortality ◽  
Natural Mortality ◽  
Hybrid Striped Bass ◽  
Morone Chrysops ◽  
Potential Benefits ◽  
Community Fisheries ◽  
Effect On Yield

<em>Abstract.-</em>Growth, exploitation, and natural mortality of walleyes <em>Sander vitreus </em>and hybrid striped bass <em>Morone chrysops </em>x <em>M. saxatilis </em>were investigated in Lake Manawa during 2005 and 2006. The major objectives were to assess the contribution of these popular sportfish species in an urban situation and to determine the potential benefits of regulation reductions. Lake Manawa is a 320 ha natural lake in Council Bluffs, Iowa. Currently, walleyes are regulated only with a creel limit of 5 and possession limit of 10, and hybrid striped bass are not regulated. Growth of walleyes (through age 8) was average to slightly above average that of other North American populations, while growth of hybrid striped bass (through age 5) was average to slightly lower. Significantly more walleyes and hybrid striped bass were caught than harvested; exploitation rates were estimated at 24% for both species. Instantaneous total mortality rates (<EM>Z</EM>) for walleyes and hybrid striped bass were less than 40%, and corresponding survival rates (<EM>S</EM>) approached 70%. Regulation models predicted that length limits on either species would have negligible effect on yield, but decrease harvest greatly. We conclude that natural mortality plays a greater role in the abundance and fishing quality of walleyes and hybrid striped bass in this urban lake than do angler effects, and regulation changes are not warranted, especially considering that usage rates indicate that education and communication efforts are not adequately promoting these fisheries.

Composition and Quality of Aquacultured Hybrid Striped Bass Fillets as Affected by Dietary Fatty Acids

1994 ◽  
Vol 59 (1) ◽  
pp. 70-75 ◽  
Author(s):  
KELLY P. FOWLER ◽  
CAROL KARAHADIAN ◽  
NEIL J. GREENBERG ◽  
REGINAL M. HARRELL
Keyword(s):  
Fatty Acids ◽  
Striped Bass ◽  
Dietary Fatty Acids ◽  
Hybrid Striped Bass

Aquatic Francisella-like bacterium associated with mortality of intensively cultured hybrid striped bass Morone chrysops × M. saxatilis

2006 ◽  
Vol 72 ◽  
pp. 135-145 ◽  
Author(s):  
VE Ostland ◽  
JA Stannard ◽  
JJ Creek ◽  
RP Hedrick ◽  
HW Ferguson ◽  
...  
Keyword(s):  
Striped Bass ◽  
Hybrid Striped Bass ◽  
Morone Chrysops

Microbiological Quality of Fresh Blue Crabmeat, Clams and Oysters

1983 ◽  
Vol 46 (11) ◽  
pp. 978-981 ◽  
Author(s):  
B. A. WENTZ ◽  
A. P. DURAN ◽  
A. SWARTZENTRUBER ◽  
A. H. SCHWAB ◽  
R. B. READ
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Geometric Means ◽  
Colony Forming Units ◽  
Plate Counts ◽  
The Mean ◽  
Eastern Oysters

The microbiological quality of fresh blue crabmeat, soft- and hardshell clams and shucked Eastern oysters was determined at the retail (crabmeat, oysters) and wholesale (clams) levels. Geometric means of aerobic plate counts incubated at 35°C were: blue crabmeat 140,000 colony-forming units (CFU)/g, hardshell clams, 950 CFU/g, softshell clams 680 CFU/g and shucked Eastern oysters 390,000 CFU/g. Coliform geometric means ranged from 3,6/100 g for hardshell clams to 21/g for blue crabmeat. Means for fecal coliforms or Escherichia coli ranged from &lt;3/100 g for clams to 27/100 g for oysters, The mean Staphylococcus aureus count in blue crabmeat was 10/g.

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