Microbiological Analysis of Striped Bass (Morone saxatilis) Grown in a Recirculating System†

1997 ◽  
Vol 60 (8) ◽  
pp. 948-953 ◽  
Author(s):  
PATTI C. NEDOLUHA ◽  
DENNIS WESTHOFF
Keyword(s):  
Striped Bass ◽  
Morone Saxatilis ◽  
Microbiological Analysis ◽  
Food Borne Pathogens ◽  
Group I ◽  
Food Borne ◽  
Vibrio Mimicus ◽  
Plate Counts ◽  
Tank Water

The skin, gills, intestines, tank water, and diet of striped bass grown in recirculating tanks were examined for quantity and quality of microflora. Plate counts for the fish and water samples were similar to counts reported in other systems. The bacteria groups represented most frequently in isolates from the fish were Aeromonas (14%), Moraxellaceae (15%), the Flavobacterium-Chryseobacterium-Cytophaga-Sphingobacterium group (11 %), Bacillus (8%), Group I Pseudomonas (6%), the Shewanella-Alteromonas group (6%), and Comamonadaceae (5%). The food-borne pathogens Staphylococcus aureus, motile Aeromonas spp., and Vibrio mimicus were isolated repeatedly. V. parahaemolyticus and V. cholera were each isolated once, and no Listeria, Plesiomonas, Salmonella, or Yersinia enterocolitica were isolated. Close taxonomic relationships were observed among some of the bacteria found on the fish and in the water; bacteria from the skin and gills were more likely to be related to each other and to the water isolates than to the intestinal isolates. Some bacteria in the intestines originated in the diet, but these strains did not significantly affect the overall microflora of the fish and water.

Microbiological Analysis of Striped Bass (Morone saxatilis) Grown in Flow-Through Tanks

1995 ◽  
Vol 58 (12) ◽  
pp. 1363-1368 ◽  
Author(s):  
PATTI C. NEDOLUHA ◽  
DENNIS WESTHOFF
Keyword(s):  
Striped Bass ◽  
Foodborne Pathogens ◽  
Morone Saxatilis ◽  
Microbiological Analysis ◽  
Bacillus Spp ◽  
Plate Counts ◽  
Flow Through ◽  
Aerobic And Anaerobic ◽  
Vibrio Spp

Aquacultured striped bass from indoor flow-through tanks were examined for quantity and quality of microflora. Plate counts were performed at three temperatures (7°C, 22°C, and 35°C) under aerobic and anaerobic conditions. Bacterial loads on the skin, gills, and intestines were similar to those reported for wild striped bass and pond-raised hybrid striped bass. The predominant groups of bacteria isolated were Aeromonas spp. (18%), Flavobacterium/Cytophaga species (15%), Comamonadaceae (15%), Plesiomonas shigelloides (13%), Moraxellaceae (6%), Bacillus spp. (4%), and an unidentified gram-negative rod (9%). Foodborne pathogens Staphylococcus aureus and Vibrio spp. were also isolated.

scholarly journals Effect of Modified Pre-Milking Sanitizing Approaches on Raw Milk Quality Obtained from the Dairy Farmers of Tawau Area, Sabah

2016 ◽  
Vol 4 (1) ◽  
pp. 5
Author(s):  
Sim Kheng Yuen ◽  
Mohammad Raisul Alam
Keyword(s):  
Microbiological Quality ◽  
Raw Milk ◽  
Total Coliform ◽  
Dairy Farmers ◽  
Food Borne Pathogens ◽  
Trace Back ◽  
Food Borne ◽  
Milk Hygiene ◽  
Small Holder

The aim of the study was to investigate the raw milk hygiene and quality among the small holder dairy farmers in Tawau area. A total of 216 samples were collected from the respective dairy farmers and milk collecting centre located at Mile 15, Tawau. Preliminary results indicated that the quality of the raw milks obtained at farm level contained were inferior with high bacteria load (> than 107 CFU/ml). The total coliform (2.9-3.8 CFU/mL) and Staphylococcus count (2.3-3.6 CFU/mL) were relatively high in certain samples. However, none of the food borne pathogens was found. Trace back study revealed that the causes of contamination were attributed by poor hygienic handling among the dairy farmers and insufficient for immediate chilling of raw milk. A significant reduction in bacteria load was observed if the raw milk chilled immediately at farm. The implementation of modified pre-milking sanitizing practices improved the microbiology quality of the raw milks obtained from respective dairy farms. Future study will focus more on the effect of prolong storage towards the microbiological quality of raw milk.

Survey of the Microbiological Quality of Whole, Black Pepper and Turmeric Powder Sold in Retail Shops in Bombay

1987 ◽  
Vol 50 (5) ◽  
pp. 401-403 ◽  
Author(s):  
H. GEETA ◽  
P. R. KULKARNI
Keyword(s):  
Microbiological Quality ◽  
Black Pepper ◽  
Poor Quality ◽  
International Standards ◽  
Microbiological Analysis ◽  
Fungal Flora ◽  
Plate Counts ◽  
The City

Microbiological analysis of loosely packed, whole, black pepper and turmeric powder obtained from retail shops in the city of Bombay revealed that the samples of both spices were highly contaminated. Aerobic plate counts of black pepper ranged from 12.1 × 107 to 81.9 × 108 c.f.u. per gram and turmeric powder from 4.1 × 107 to 73.6 × 108 c.f.u. per g. In both spices, mesophilic sporeformers like Bacillus occurred. Coliforms ranged in counts from 102-103 per g. Fungal counts ranged from 0.6 × 104 to 16 × 105 per g for black pepper and from 0.5 × 103 to 11.1 × 105 per g for turmeric powder. Fungal flora included mainly Aspergillus spp. with the occurrence of Mucor in some of the samples. No other organisms were observed in the dilutions plated. The extent of contamination was slightly greater in pepper than in turmeric, although both spices were of a poor quality when compared with international standards.

scholarly journals Bacteriological quality of commercially prepared fermented Ogi (Akamu) sold in Some Parts of Afikpo Area in Ebonyi State

2020 ◽  
Vol 4 (3) ◽  
pp. 077-081
Author(s):  
Ojokoh Eromosele ◽  
Ndukwe Chima K ◽  
Omonu Richard ◽  
Asadu Kenneth ◽  
Okpara Kingsley O
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Method ◽  
E Coli ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Bacteriological Test ◽  
Local Areas ◽  
Processing And Storage ◽  
And Storage

In this study ogi (akamu) prepared from fermented maize sold in Enohia, Afikpo and Unwana were subjected to bacteriological test together with the laboratory prepared ogi for bacterial quality using the standard microbiological method. The commercial purchased ogi from the markets of (Enohia, Unwana and Afikpo) showed total bacterial growth of 5.6 × 107, 2.0 × 103, 4.2 × 102 and 3.6 × 106 respectively. Staphylococci growth count of 4.2×102, 2.9×102 and 3.5 × 102 were recorded respectively, while Coliform count recorded 2.0×103, 1.2×103 and 1.5×103 and Lactic acid bacterial loads of 3.6×106, 2.7×106 and 3.2 × 106 respectively. The bacteria isolated from the commercial and laboratory fermented pap were Lactobacillus sp, Staphylococcus sp, Leuconostoc sp, Micrococcus sp, Salmonella sp, E. coli, Citrobacter sp and Klebsiella sp. Lactobacillus sp Leuconostoc sp and Citrobacter sp were present in all the samples, Micrococcus sp and Klebsiella sp were isolated from Enohia and Unwana markets ogi respectively, while E. coli and Staphylococcus sp were present in Enohia, Unwana and Afikpo markets. Salmonella sp were isolated from the three markets. No pathogenic bacteria was isolated from the laboratory Prepared ogi (Akamu). Hence, there are chances of contracting food borne diseases from commercially prepared ogi in the local areas of study. Therefore, there is need for sanitary measures in the production of fermented cereals such as ogi so as to minimize the rate of food borne pathogens during processing and storage of such cereals.

Microbiological Flora of Aquacultured Hybrid Striped Bass

1993 ◽  
Vol 56 (12) ◽  
pp. 1054-1060 ◽  
Author(s):  
PATTI C. NEDOLUHA ◽  
DENNIS WESTHOFF
Keyword(s):  
Striped Bass ◽  
Foodborne Pathogens ◽  
Yersinia Pseudotuberculosis ◽  
Wild Fish ◽  
Shigella Dysenteriae ◽  
Hybrid Striped Bass ◽  
Bacillus Spp ◽  
Plate Counts ◽  
Aerobic And Anaerobic

Aquacultured hybrid striped bass were examined for quantity and quality of microflora. Plate counts were performed at three temperatures (7, 22, and 35°C) under aerobic and anaerobic conditions. Bacterial loads on the skin, gills, and intestines were similar to those reported for wild fish. Plate counts performed at 22°C yielded the highest counts and the greatest variety of species. The predominant groups of bacteria isolated were Aeromonas spp. (27%), coryneforms (14%), Pseudomonas spp. (12%), Flavobacterium/Cytophaga/Sphingobacterium group (8%), Plesiomonas shigelloides (7%), Bacillus spp. (7%), and Enterobacteriaceae (6%). Human foodborne pathogens Listeria monocytogenes, Staphylococcus aureus, Shigella dysenteriae, Vibrio spp., and Yersinia pseudotuberculosis were also isolated. There were no indications, however, that pond-raised fish present a higher risk of foodborne illness than do wild fish.

scholarly journals Microbiological Analysis, Cyanide and Moisture Content of Different Garri Samples

2019 ◽  
pp. 1-4
Author(s):  
C. E. Kalu
Keyword(s):  
Moisture Content ◽  
Microbiological Analysis ◽  
Food Borne ◽  
Food Standard ◽  
Plate Counts ◽  
Safe Limits ◽  
Microbial Analysis ◽  
Moisture Content Determination ◽  
Staphylococcus Spp ◽  
Cyanide Content

Three garri samples from three differents markets in Afikpo North L.G.A of Ebonyi State were collected and the microbial, moisture content and cyanide content of the samples (A, B, C) were assayed to ascertain their quality and safety. The Microbial analysis, the total plate counts were 7.9 x 105 cfu/g, 2.6 x 105 cfu/g, and 1.6 x105 cfu/g in samples A, B and C respectively. On the other hand the fungal counts were 1.6 x105 cfu/g, 0.8 x105 cfu/g and 0.2 x 105 cfu/g in samples A, B and C respectively. Staphylococcus spp was isolated from all the garri samples while streptococcus spp was isolated in samples A and B. However Escherichia coli was isolated only in sample A. The fungi isolated were Aspergillus spp in samples A and B while Penicillium spp was isolated in all samples A, B and C. Cyanide content of the samples were also determined. The values were 0.688 mg/kg, 0.750 mg/kg and 0.630 mg/kg in samples A, B, and C respectively. The moisture content determination of the garria the values of 16%, 14% and 13% in samples A, B, and C respectively. Thus, the microbial counts of the garri samples were within safe limits of the international food standard (<105 cfu/g). The cyanide contents were also within safe limits of the international standard (<IHCH/100 g). Moreover, the absence of food borne pathogens of microbiological safe.

Assessment of Microbiological Quality of Retail Fresh Sushi from Selected Sources in Norway

2015 ◽  
Vol 78 (5) ◽  
pp. 977-982 ◽  
Author(s):  
SUNNIVA HOEL ◽  
LISBETH MEHLI ◽  
TORKJEL BRUHEIM ◽  
OLAV VADSTEIN ◽  
ANITA NORDENG JAKOBSEN
Keyword(s):  
Temperature Control ◽  
Pathogenic Bacteria ◽  
Raw Materials ◽  
Microbiological Quality ◽  
Microbiological Analysis ◽  
Follow Up Study ◽  
Plate Counts ◽  
Initial Sampling

Retail fresh sushi is gaining popularity in Europe. This study was conducted to investigate the microbiological quality of selected samples of fresh sushi with a shelf life of 2 to 3 days offered as complete meals in Norwegian supermarkets. Analysis of aerobic plate counts in 58 sushi samples from three producers revealed large variations in microbiological quality, and 48% of the analyzed sushi boxes were rated as unsatisfactory (&gt; 6.0 log CFU/g). Mesophilic Aeromonas spp. was detected in 71% of the samples. In a follow-up study, we collected products and raw materials directly from the production facility of one producer and observed a significant decrease (P &lt; 0.01) in aerobic plate counts compared with the initial sampling. The observed difference between products purchased in stores compared with those collected directly from the factory suggests that poor temperature control during distribution and display in stores leads to reduced microbiological quality. Microbiological analysis of the sushi ingredients revealed that potentially pathogenic bacteria such as mesophilic Aeromonas spp. or bacteria belonging to the Enterobacteriaceae can be introduced into sushi through both raw vegetables and fish. The results highlight the importance of high quality ingredients and proper temperature control to ensure stable quality and safety of these food products.

Emamectin benzoate is a safe and effective anthelmintic against coelomic nematode Philometra rubra in striped bass Morone saxatilis

2020 ◽  
Vol 142 ◽  
pp. 47-53
Author(s):  
K Béland ◽  
G Séguin ◽  
S Lair
Keyword(s):  
Mortality Rate ◽  
Striped Bass ◽  
High Mortality ◽  
Morone Saxatilis ◽  
Treatment Results ◽  
Emamectin Benzoate ◽  
Once Daily ◽  
Fish Hatchery ◽  
Parasitic Burden

An unusually high mortality rate due to verminous (Philometra rubra) coelomitis was documented in wild-hatched striped bass Morone saxatilis raised in a fish hatchery as part of a stock restoration program. To decrease the parasitic burden and therefore potentially minimize mortality, the effectiveness of 2 different anthelmintics was evaluated. Two trials were conducted on wild-collected fingerlings naturally infected by P. rubra. In 2006, 144 yearling fish were randomly assigned to 4 experimental groups: (1) levamisole (Levasol®) at 2 mg l-1 via immersion for 8 h once weekly for 3 wk; (2) levamisole at a dose of 2.5 mg kg-1 biomass via feed once daily for 7 d; (3) emamectin benzoate (Slice®) at a dose of 0.05 mg kg-1 biomass via feed once daily for 7 d; and (4) control. Emamectin successfully eliminated live nematodes in 84.9% of the fish, whereas the administration of levamisole, either via immersion or feed, was not successful in significantly reducing the number of live P. rubra. In 2007, the administration of the same dosage of emamectin to approximately 1000 naturally infected yearling striped bass was associated with a 100% mortality rate of P. rubra in the 30 fish randomly examined 5 wk after the beginning of the treatment. Results of these trials indicate that, at the dosage used, the administration of emamectin at the end of the summer is safe for striped bass yearlings and considerably reduces the prevalence and intensity of the infection by this parasite.

Nerve-sparing radical hysterectomy in patients with infiltrative cervical cancer

2016 ◽  
pp. 46-51
Author(s):  
T. Dermenzhy ◽  
◽  
V. Svintitskiy ◽  
S. Nespryadko ◽  
L. Legerda ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Radical Hysterectomy ◽  
Contractile Function ◽  
Major Elements ◽  
Control Group ◽  
Nerve Sparing ◽  
Group I ◽  
Hypogastric Plexus ◽  
Prognostic Indexes

The objective: to improve an effectiveness of therapy and quality of life of patients with infiltrative cervical cancer using radical hysterectomy accomplished with nerve-sparing methodology. Patients and Methods: Ninety patients with histologically verified infiltrative cervical cancer were cured with radical hysterectomy (RHE) in the Department of Oncogynecology of National Cancer Institute (Kyiv, Ukraine) in 2012-2016. The age of the patients was from 26 to 65 years (an average age of 42.61±1.06). The patients were distributed in 2 groups: group I treated with nerve-sparing radical hysterectomy (NSRHE), 45 patients, the main group; group II treated with radical hysterectomy (RHE III), the control group, 45 patients. The prognostic indexes in the groups were similar. Results. NSRHE that included the dissection of cardinal ligament, separation of dorsal and anterior layers of uterovesical ligament allowed separate uterine branch of inferior hypogastric plexus, preserve an innervation of urinary bladder and prevent the malfunction of its contractile function at postoperative period. Conclusion. The data of the urodynamic study using cystomanometry performed at pre- and early operative periods have shown that surgical treatment of patients with infiltrative cervical cancer with preservation of the major elements of pelvic autonomic plexuses allows significantly decrease the rate of postoperative urogenical malfunctions. Key words: nerve-sparing radical hysterectomy, cervical cancer, cystomanometry.

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