Interaction of Salmonella enterica Subspecies enterica Serovar Typhimurium and Mung Bean (Phaseolus aureus) Plants

2005 ◽  
Vol 68 (3) ◽  
pp. 476-481 ◽  
Author(s):  
BHOJ RAJ SINGH ◽  
MUDIT CHANDRA ◽  
RAVIKANT AGARWAL
Keyword(s):  
Salmonella Typhimurium ◽  
Salmonella Enterica ◽  
Mung Bean ◽  
Room Temperature ◽  
Basal Area ◽  
Phaseolus Aureus ◽  
Sterile Soil ◽  
Aerial Parts ◽  
Serovar Typhimurium ◽  
Germinating Seeds

The effect of Salmonella enterica subspecies enterica serovar Typhimurium, a zoonotic serovar, on mung bean (Phaseolus aureus) cultivar Pant Mung-3 plants was studied. Inoculation of mung bean seeds with Salmonella Typhimurium (7.2 × 105 CFU/ml) reduced sprouting rate (P < 0.07). This effect was more pronounced at higher levels of contamination. In the soil inoculated with Salmonella Typhimurium (7.2 × 106 CFU/g), germination was retarded and the number of defective sprouts was also significantly higher (P < 0.002). Salmonella Typhimurium grew inside germinating seeds and plant tissues and persisted in seedlings, adult plants, and harvested seedlings dried and stored at room temperature (30°C) up to 45 days. Phaseolus aureus plants grown in sterile soil was resistant to Salmonella Typhimurium infection at 15 days of age and cleared Salmonella from all the aerial parts within 3 h of infection. However, Salmonella Typhimurium could be reisolated from the basal area of the stem and from soil even after 45 days of exposure to the pathogen.

Fate of Staphylococcus aureus, Salmonella enterica Serovar Typhimurium, and Vibrio vulnificus in Raw Oysters Treated with Chitosan

2006 ◽  
Vol 69 (7) ◽  
pp. 1600-1604 ◽  
Author(s):  
PALLAVI CHHABRA ◽  
YAO-WEN HUANG ◽  
JOSEPH F. FRANK ◽  
REVIS CHMIELEWSKI ◽  
KEITH GATES
Keyword(s):  
Staphylococcus Aureus ◽  
Hydrochloric Acid ◽  
Salmonella Typhimurium ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Vibrio Vulnificus ◽  
Storage Period ◽  
Major Effect ◽  
Chitosan Concentration ◽  
Serovar Typhimurium

The fate of Staphylococcus aureus, Salmonella enterica serovar Typhimurium, and Vibrio vulnificus in oysters treated with chitosan was investigated. Three concentrations (0.5, 1.0, and 2.0%) of chitosan in 0.5% hydrochloric acid were prepared and coated onto raw oysters, which were then stored at 4°C for 12 days. Untreated oysters and oysters coated with 0.5% hydrochloric acid without chitosan were used as controls. S. aureus cells were most sensitive to 2.0% chitosan followed by 0.5 and 1.0%. In general, chitosan treatment of oysters produced a decline in the population of S. aureus by 1 to 4 log CFU/ml compared with the untreated control. Chitosan treatment had no influence on the reduction of Salmonella Typhimurium over the 12-day storage period; inhibition of Salmonella Typhimurium growth was similar in both the control samples and the chitosan-treated samples. However, time of storage had a major effect on the survival of Salmonella Typhimurium on oysters. Neither time nor chitosan concentration had a significant effect on the growth of V. vulnificus during storage. All treatments were similar in inhibiting V. vulnificus growth.

MDR Salmonella enterica serovar Typhimurium ST34 carrying mcr-1 isolated from cases of bloodstream and intestinal infection in children in China

2019 ◽  
Vol 75 (1) ◽  
pp. 92-95 ◽  
Author(s):  
Qixia Luo ◽  
Fen Wan ◽  
Xiao Yu ◽  
Beiwen Zheng ◽  
Yunbo Chen ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Ecological Niches ◽  
Salmonella Infection ◽  
Serovar Typhimurium ◽  
Multiple Resistance Genes ◽  
Tn7 Transposition

Abstract Objectives Children are vulnerable to Salmonella infection due to their immature immune system. Cases of infection with mcr-1-harbouring Salmonella in child inpatients have not been reported in China before. Methods Salmonella isolates from gastroenteritis and bacteraemia were screened using primers targeting mcr-1. Complete genome sequences of mcr-1-harbouring isolates were determined using the PacBio RS II platform. The transferability of mcr-1-harbouring plasmids was verified by conjugation. Results We investigated two mcr-1-carrying polymyxin-resistant Salmonella enterica serovar Typhimurium ST34 isolates, S61394 and S44712, from bloodstream and intestinal Salmonella infection of two child inpatients, respectively. Both isolates were non-susceptible to commonly used antibiotics for children that compromised the success of clinical treatment and infection control. The mcr-1-harbouring plasmids pLS61394-MCR and pLS44712-MCR (from S61394 and S44712, respectively) were both conjugative pHNSHP45-2-like IncHI2-type epidemic plasmids carrying multiple resistance genes. Compared with pHNSHP45-2, a ∼33 kb insertion region encoding Tn7 transposition protein and heavy metal resistance proteins was identified in pLS61394-MCR, which might enhance adaptation of bacteria carrying this plasmid to various ecological niches. The phylogenetic tree of worldwide mcr-harbouring Salmonella indicated a host preference of mcr and a worldwide and cross-sectoral prevalence of the mcr-positive Salmonella ST34 clone. Conclusions To our knowledge, for the first time we report completed whole genomes of mcr-1-positive MDR Salmonella Typhimurium ST34 isolated from infected children in China, suggesting that improved surveillance is imperative for tackling the dissemination of mcr-harbouring MDR Salmonella Typhimurium ST34.

Lactic acid bacteria and bifidobacteria attenuate the proinflammatory response in intestinal epithelial cells induced by Salmonella enterica serovar Typhimurium

2013 ◽  
Vol 59 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Christine M. Carey ◽  
Magdalena Kostrzynska
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Salmonella Typhimurium ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Farm Animals ◽  
Bifidobacterium Adolescentis ◽  
Lactobacillus Kefir ◽  
Serovar Typhimurium ◽  
Ht 29

Inflammation is a physiological response to infections and tissue injury; however, abnormal immune responses can give rise to chronic inflammation and contribute to disease progression. Various dietary components, including probiotic lactic acid bacteria and prebiotics, have the potential to modulate intestinal inflammatory responses. One factor in particular, the chemokine interleukin-8 (IL-8, CXCL-8), is one of the major mediators of the inflammatory response. The purpose of this study was to investigate modulation of the inflammatory host response induced by Salmonella enterica serovar Typhimurium DT104 in the presence of selected probiotics and lactic acid bacteria (LAB) isolated from human sources, dairy products, and farm animals. IL-8 gene expression and protein production in HT-29 cells were evaluated by real-time PCR and ELISA, respectively. Pre-incubation of HT-29 cells with Lactobacillus kefir IM002, Bifidobacterium adolescentis FRP 61, Bifidobacterium longum FRP 68 and FRP 69, Bifidobacterium breve FRP 334, and Leuconostoc mesenteroides IM080 significantly inhibited IL-8 secretion induced by Salmonella Typhimurium DT104. Co-culture of selected probiotics and Salmonella Typhimurium DT104 reduced IL-8 production, while potential probiotics and LAB had no effect on IL-8 secretion in HT-29 cells preincubated with Salmonella Typhimurium DT104 prior to adding probiotics. Lactobacillus kefir IM002 supernatant also significantly reduced IL-8 production. In conclusion, our study suggests that probiotic bifidobacteria and LAB modulate cytokine induction and possess anti-inflammatory properties; however, the effectiveness is strain dependent.

scholarly journals Explosive increase of multiresistant Salmonella Typhimurium DT104 in 2001 in the Netherlands

2001 ◽  
Vol 5 (50) ◽  
Author(s):  
W van Pelt
Keyword(s):  
Public Health ◽  
The Netherlands ◽  
Salmonella Typhimurium ◽  
Salmonella Enterica ◽  
Phage Type ◽  
Serovar Typhimurium

From September 2000 and up until the end of that year, the national salmonella centre at the Rijksinstituut voor Volksgezondheid en Milieu (RIVM, the National Institute of Public Health and the Environment) has noted an explosive increase of isolates of Salmonella enterica subspecies enterica serovar Typhimurium definitive phage type (DT) 104 from humans, which has since plateaued at a level double that of previous years (1).

scholarly journals DNA Amplification and Rearrangements in Archival Salmonella enterica Serovar Typhimurium LT2 Cultures

2004 ◽  
Vol 186 (6) ◽  
pp. 1678-1682 ◽  
Author(s):  
Steffen Porwollik ◽  
Rita Mei-Yee Wong ◽  
R. Allen Helm ◽  
Kelly K. Edwards ◽  
Michael Calcutt ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Room Temperature ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Dna Amplification ◽  
Genomic Rearrangements ◽  
Pcr Analysis ◽  
Serovar Typhimurium ◽  
Over 40 Years ◽  
Over Time ◽  
Genomic Duplication

ABSTRACT Variations in genome size and gene order were observed in archival Salmonella enterica serovar Typhimurium cultures stored for over 40 years. In one strain, microarray analysis revealed a large, stable amplification. PCR analysis of the same strain revealed a genomic duplication that underwent a translocation. Other strains had smaller duplications and deletions. These results demonstrate that storage in stabs over time at room temperature not only allows for further bacterial growth but also may produce an environment that selects for a variety of mutations, including genomic rearrangements.

Experimental Use of 2-Nitropropanol for Reduction of Salmonella Typhimurium in the Ceca of Broiler Chicks†‡

2004 ◽  
Vol 67 (9) ◽  
pp. 1945-1947 ◽  
Author(s):  
YONG SOO JUNG ◽  
ROBIN C. ANDERSON ◽  
THOMAS S. EDRINGTON ◽  
KENNETH J. GENOVESE ◽  
J. ALLEN BYRD ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Salmonella Typhimurium ◽  
Inhibitory Activity ◽  
Salmonella Enterica ◽  
Volatile Fatty Acid ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Broiler Chicks ◽  
Serovar Typhimurium ◽  
Experimental Use

The effect of 2-nitropropanol (2NPOH) administration on Salmonella enterica serovar Typhimurium in experimentally infected chicks was determined. Chicks orally challenged with 106 CFU/ml of a novobiocin- and naladixic acid–resistant Salmonella Typhimurium at 6 days of age were divided into three groups receiving 0 (control), 6.5, and 13 mg 2NPOH per bird (experiment 1) or four groups receiving 0 (control), 13, 65, and 130 mg 2NPOH per bird (experiment 2). Treatments were administered orally 1 day post–Salmonella challenge. Cecal contents collected at necropsy 24 and 48 h after treatment were subjected to bacterial and volatile fatty acid (VFA) analysis. In experiment 1, concentrations (mean ± SD log CFU per g) of Salmonella were reduced (P < 0.05) in the group administered 13 mg 2NPOH per bird at both the 24- and 48-h samplings compared with the controls (2.58 ± 2.10 versus 4.64 ± 1.79 and 2.88 ± 2.78 versus 5.03 ± 2.42 at 24 and 48 h, respectively). In experiment 2, mean ± SD populations of Salmonella were reduced (P < 0.05) in all groups receiving 2NPOH compared with untreated controls (3.65 ± 2.01, 3.39 ± 2.42, and 3.47 ± 1.55 at 13, 65, and 130 mg, respectively, versus 6.09 ± 1.02). Propionate concentrations were reduced (P < 0.05) by the 13-mg 2NPOH per bird treatment. Total VFA concentrations from the group treated with 13 mg 2NPOH per bird were lower (P < 0.05) by 48, but not 24, hours posttreatment than those from the group treated with 6.5 mg 2NPOH per bird. These results demonstrate the inhibitory activity of 2NPOH against Salmonella Typhimurium in vivo.

scholarly journals Genomic Diversification among Archival Strains of Salmonella enterica Serovar Typhimurium LT7

2003 ◽  
Vol 185 (7) ◽  
pp. 2131-2142 ◽  
Author(s):  
Gui-Rong Liu ◽  
Kelly Edwards ◽  
Abraham Eisenstark ◽  
Ying-Mei Fu ◽  
Wei-Qiao Liu ◽  
...  
Keyword(s):  
Rapid Growth ◽  
Colony Size ◽  
Salmonella Enterica ◽  
Room Temperature ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Genome Structure ◽  
Point Mutations ◽  
Mutator Strain ◽  
Genomic Changes ◽  
Serovar Typhimurium

ABSTRACT To document genomic changes during long periods of storage, we analyzed Salmonella enterica serovar Typhimurium LT7, a mutator strain that was previously reported to have higher rates of mutations compared to other serovar Typhimurium strains such as LT2. Upon plating directly from sealed agar stabs that had been stocked at room temperature for up to four decades, many auxotrophic mutants derived from LT7 gave rise to colonies of different sizes. Restreaking from single colonies consistently yielded colonies of diverse sizes even when we repeated single-colony isolation nine times. Colonies from the first plating had diverse genomic changes among and even within individual vials, including translocations, inversions, duplications, and point mutations, which were detected by rare-cutting endonuclease analysis with pulsed-field gel electrophoresis. Interestingly, even though the colony size kept diversifying, all descendents of the same single colonies from the first plating had the same sets of detected genomic changes. We did not detect any colony size or genome structure diversification in serovar Typhimurium LT7 stocked at −70°C or in serovar Typhimurium LT2 stocked either at −70°C or at room temperature. These results suggest that, although colony size diversification occurred during rapid growth, all detected genomic changes took place during the storage at room temperature and were carried over to their descendents without further changes during rapid growth in rich medium. We constructed a genomic cleavage map on the LT7 strain that had been stocked at −70°C and located all of the detected genomic changes on the map. We speculated on the significance of mutators for survival and evolution under environmentally stressed conditions.

scholarly journals Caracterización genética y patrones de resistencia antimicrobiana en cepas de Salmonella enterica subsp. enterica serovar Typhimurium en cuyes de crianza intensiva

2020 ◽  
Vol 31 (1) ◽  
pp. e17542
Author(s):  
Meylin Huamán ◽  
Crhistian Pérez ◽  
Jorge Rodríguez ◽  
Marjorie Killerby ◽  
Stephane Lovón ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Salmonella Enterica ◽  
Serovar Typhimurium

El objetivo del estudio fue realizar la caracterización fenotípica y genética de 35 aislados de Salmonella Typhimurium provenientes de sistemas de producción de cuyes en la región Lima, Perú, con relación a la resistencia a antimicrobianos. Se determinó el perfil de 11 antibióticos (florfenicol, sulfametoxazol, doxiciclina, oxitetraciclina, amoxicilina, enrofloxacina, norfloxacina, levofloxacina, ciprofloxacina, colistina y fosfomicina), genotipificación por técnicas de ERIC-PCR y perfil de genes de resistencia a quinolonas (qnrB, qnrD, qnrR, aa6), tetraciclinas (tetA, tetB, tetC, tetD), fenicoles (cat1, cat2, cmlA, cmlB) y sulfametoxazol (sul1, sul2). Los resultados indican la presencia de multidrogo resistencia en un 80% (n=28) de las cepas, siendo la resistencia más común al antibiótico colistina (91.4%), seguido del sulfametoxazol (68.6%) y enrofloxacina (62.9%), y con una moderada resistencia a amoxicilina (20%), ciprofloxacina (20%) y norfloxacina (11.43%). Nueve de 14 genes de resistencia fueron detectados, con una mayor frecuencia de los genes tetB (71.4%), sulI (57.1%) y cat2 (48.6%). Se observó la presencia de siete perfiles genéticos diferenciados (A-G) distribuidos en dos clústeres genéticos, siendo el perfil D más frecuente (54.3%) seguido del perfil C (28.6%) de frecuencia. Los resultados sugieren la presencia de cepas de Salmonella Typhimurium con moderada variabilidad y perfiles de multidrogo resistencia con la presencia de genes de resistencia, principalmente para tetraciclinas y sulfonamidas.

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