Dietary Proteins Extend the Survival of Salmonella Dublin in a Gastric Acid Environment

2012 ◽  
Vol 75 (2) ◽  
pp. 353-358 ◽  
Author(s):  
TINA BIRK ◽  
KIM KRISTENSEN ◽  
ANNE HARBOE ◽  
TINA BECK HANSEN ◽  
HANNE INGMER ◽  
...  
Keyword(s):  
Gastric Acid ◽  
Foodborne Pathogen ◽  
Gastric Fluid ◽  
Human Stomach ◽  
Pathogen Inactivation ◽  
Related Factors ◽  
Salmonella Dublin ◽  
Food Components ◽  
Set Up ◽  
The Impact

The pH of the human stomach is dynamic and changes over time, depending on the composition of the food ingested and a number of host-related factors such as age. To evaluate the number of bacteria surviving the gastric acid barrier, we have developed a simple gastric acid model, in which we mimicked the dynamic pH changes in the human stomach. In the present study, model gastric fluid was set up to imitate pH dynamics in the stomachs of young and elderly people after ingestion of a standard meal. To model a serious foodborne pathogen, we followed the survival of Salmonella enterica serotype Dublin, and found that the addition of proteins such as pepsin, ovalbumin, and blended turkey meat to the simple gastric acid model significantly delayed pathogen inactivation compared with the control, for which no proteins were added. In contrast, no delay in inactivation was observed in the presence of bovine serum albumin, indicating that protection could be protein specific. The simple gastric acid model was validated against a more laborious and complex fermenter model, and similar survival of Salmonella Dublin was observed in both models. Our gastric acid model allowed us to evaluate the influence of food components on survival of pathogens under gastric conditions, and the model could contribute to a broader understanding of the impact of specific food components on the inactivation of pathogens during gastric passage.

scholarly journals New strategies for the control of infectious and parasitic diseases in blood donors: the impact of pathogen inactivation methods

2020 ◽  
Vol 4 (2) ◽  
pp. 53-66
Author(s):  
Luca Galli ◽  
Fabrizio Bruschi
Keyword(s):  
Blood Donors ◽  
Parasitic Diseases ◽  
Infectious Agents ◽  
Blood Components ◽  
Pathogen Inactivation ◽  
Blood Safety ◽  
New Agents ◽  
Set Up ◽  
The Impact ◽  
New Strategies

AbstractAround 70 infectious agents are possible threats for blood safety.The risk for blood recipients is increasing because of new emergent agents like West Nile, Zika and Chikungunya viruses, or parasites such as Plasmodium and Trypanosoma cruzi in non-endemic regions, for instance.Screening programmes of the donors are more and more implemented in several Countries, but these cannot prevent completely infections, especially when they are caused by new agents.Pathogen inactivation (PI) methods might overcome the limits of the screening and different technologies have been set up in the last years.This review aims to describe the most widely used methods focusing on their efficacy as well as on the preservation integrity of blood components.

scholarly journals Prior Exposure to Dry-Cured Meat Promotes Resistance to Simulated Gastric Fluid in Salmonella Typhimurium

Foods ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 603 ◽  
Author(s):  
Yhan S. Mutz ◽  
Denes K. A. Rosario ◽  
Vinicius S. Castro ◽  
Patricia C. Bernardes ◽  
Vania M. F. Paschoalin ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Salmonella Enterica ◽  
Meat Product ◽  
Foodborne Pathogen ◽  
Acid Tolerance ◽  
Gastric Fluid ◽  
Simulated Gastric Fluid ◽  
Pathogen Inactivation ◽  
Cured Meat

This study assessed if exposure of foodborne Salmonella enterica in Brazilian dry-cured loin (BDL) affects pathogen inactivation in simulated gastric fluid (SGF). The acid tolerance responses of three Salmonella enterica serovars, Typhimurium, Derby and Panama, were assessed by an acid challenge trial at pH 3.0 for 4 h following pre-adaptation to three conditions: neutral pH, acidic pH (4.5) or BDL matrix. The influence of Salmonella exposure temperature and time in the BDL on pathogen gastric fluid resistance was evaluated by the response surface methodology. The Salmonella serovars acquired acid tolerance when exposed to the BDL matrix and their response to acid stress was strain-dependent, with S. Typhimurium being the most tolerant strain. S. Typhimuirum exposed to temperatures >25 °C in the BDL matrix displayed increased resistance to SGF. By using the response surface methodology, it was determined that S. Typhimurium becomes less resistant against SGF if maintained in the BDL matrix at temperatures <7 °C, reinforcing the recommendation to store dry-cured meat under refrigeration in order to minimize consumer risks. The results presented herein point to a novel aspect of hurdle technology that should be taken into account to further understand the risks associated with hurdle-stable meat product, such as dry-cured meats, concerning foodborne pathogen contamination.

A Study On Impact Of Work Life Related Factors On Employee Engagement In Health Care Sector Workers In Dindigul District.

2019 ◽  
Vol 118 (7) ◽  
pp. 20-26
Author(s):  
S. JAYARAMAN ◽  
R. Sindhya ◽  
P. Vijiyalakshmi
Keyword(s):  
Health Care ◽  
Path Analysis ◽  
Employee Engagement ◽  
Health Care Workers ◽  
Health Care Sector ◽  
Work Life ◽  
Primary Data ◽  
Related Factors ◽  
Care Workers ◽  
The Impact

this research aims to find out the intensity of Employee Engagement of the health care sector workers and the relationship between the Work life factors and Employee Engagement of Health care sector workers in Dindigul District. Primary data were used in this research, were collected from 298 Health care workers from Dindigul District. Questionnaire was the major tool used to gather the primary data from the selected sample respondents. For this purpose, a well structured questionnaire was constructed with the help of professionals and the practiced employees of various health care units in Dindigul District. The health care employees were chosen by simple random sampling method. The investigative measures of regression Path analysis, and simple percentage analysis were utilized to find the impact of work life related factors with the Employee Engagement. The maximum Health care workers were generally satisfied with their jobs. The analytical procedure of path analysis multiple regressions was utilized to determine the predicting strength among Work life factors and the employee engagement. This study provides an another view about the importance of Work life factors and Employee engagement for organizational effectiveness and performance .

MOTIF DAN DAMPAK PERNIKAHAN DINI DI INDARUNG NGALAU BATU GADANG

2018 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Murisal Murisal
Keyword(s):  
Young Men ◽  
Early Marriage ◽  
Marriage Law ◽  
Men And Women ◽  
Home Life ◽  
Family Household ◽  
Set Up ◽  
The Impact ◽  
Husband And Wife

Motif and Impact of Early Marriage in Indarung Ngalau Batu Gadang.Penelitian is motivated by teenagers who married early on. Today, young men and women have a tendency to be less prepared to enter the home life, they are only ready to marry (ready here can be interpreted, maturity in terms of financial, understand what the meaning of marriage according to marriage law) is the bond of inner birth between a man and a woman as husband and wife for the purpose of forming a happy and eternal family (household) based on the Supreme Godhead while they are not ready to set up a home, whereas to build a household requires preparation both physically and spiritually . The purpose of this study to determine the motives underlying adolescents to make early marriage and the impact caused in the household as a result of the marriage.

Dissent and Education

2018 ◽  
Author(s):  
Mark Burden
Keyword(s):  
Eighteenth Century ◽  
Seventeenth Century ◽  
Educational Activity ◽  
Educational Institutions ◽  
Higher Educational Institutions ◽  
Legal Restrictions ◽  
Higher Educational ◽  
Set Up ◽  
The Impact ◽  
Dissenting Academies

Much eighteenth-century Dissenting educational activity was built on an older tradition of Puritan endeavour. In the middle of the seventeenth century, the godly had seen education as an important tool in spreading their ideas but, in the aftermath of the Restoration, had found themselves increasingly excluded from universities and schools. Consequently, Dissenters began to develop their own higher educational institutions (in the shape of Dissenting academies) and also began to set up their own schools. While the enforcement of some of the legal restrictions that made it difficult for Dissenting institutions diminished across the eighteenth century, the restrictions did not disappear entirely. While there has been considerable focus on Dissenting academies and their contribution to debates about doctrinal orthodoxy, the impact of Dissenting schools was also considerable.

scholarly journals On Kaufmann's theory of the impact of the pianoforte hammer

1920 ◽  
Vol 97 (682) ◽  
pp. 99-110 ◽  
Keyword(s):  
Initial Conditions ◽  
Practical Importance ◽  
Prima Facie ◽  
Point Of View ◽  
Infinite Length ◽  
Modes Of Vibration ◽  
Rigorous Treatment ◽  
The Subject ◽  
Set Up ◽  
The Impact

The theory of the vibrations of the pianoforte string put forward by Kaufmann in a well-known paper has figured prominently in recent discussions on the acoustics of this instrument. It proceeds on lines radically different from those adopted by Helmholtz in his classical treatment of the subject. While recognising that the elasticity of the pianoforte hammer is not a negligible factor, Kaufmann set out to simplify the mathematical analysis by ignoring its effect altogether, and treating the hammer as a particle possessing only inertia without spring. The motion of the string following the impact of the hammer is found from the initial conditions and from the functional solutions of the equation of wave-propagation on the string. On this basis he gave a rigorous treatment of two cases: (1) a particle impinging on a stretched string of infinite length, and (2) a particle impinging on the centre of a finite string, neither of which cases is of much interest from an acoustical point of view. The case of practical importance treated by him is that in which a particle impinges on the string near one end. For this case, he gave only an approximate theory from which the duration of contact, the motion of the point struck, and the form of the vibration-curves for various points of the string could be found. There can be no doubt of the importance of Kaufmann’s work, and it naturally becomes necessary to extend and revise his theory in various directions. In several respects, the theory awaits fuller development, especially as regards the harmonic analysis of the modes of vibration set up by impact, and the detailed discussion of the influence of the elasticity of the hammer and of varying velocities of impact. Apart from these points, the question arises whether the approximate method used by Kaufmann is sufficiently accurate for practical purposes, and whether it may be regarded as applicable when, as in the pianoforte, the point struck is distant one-eighth or one-ninth of the length of the string from one end. Kaufmann’s treatment is practically based on the assumption that the part of the string between the end and the point struck remains straight as long as the hammer and string remain in contact. Primâ facie , it is clear that this assumption would introduce error when the part of the string under reference is an appreciable fraction of the whole. For the effect of the impact would obviously be to excite the vibrations of this portion of the string, which continue so long as the hammer is in contact, and would also influence the mode of vibration of the string as a whole when the hammer loses contact. A mathematical theory which is not subject to this error, and which is applicable for any position of the striking point, thus seems called for.

scholarly journals Impact of Air Infiltration on IAQ and Ventilation Efficiency in Higher Educational Classrooms in Spain

2021 ◽  
Vol 13 (12) ◽  
pp. 6875
Author(s):  
Irene Poza-Casado ◽  
Raquel Gil-Valverde ◽  
Alberto Meiss ◽  
Miguel Ángel Padilla-Marcos
Keyword(s):  
Natural Ventilation ◽  
Well Being ◽  
Cold Season ◽  
Climate Conditions ◽  
Air Infiltration ◽  
Ventilation Efficiency ◽  
Educational Buildings ◽  
Set Up ◽  
Total Volatile Organic Compounds ◽  
The Impact

Indoor air quality (IAQ) in educational buildings is a key element of the students’ well-being and academic performance. Window-opening behavior and air infiltration, generally used as the sole ventilation sources in existing educational buildings, often lead to unhealthy levels of indoor pollutants and energy waste. This paper evaluates the conditions of natural ventilation in classrooms in order to study how climate conditions affect energy waste. For that purpose, the impact of the air infiltration both on the IAQ and on the efficiency of the ventilation was evaluated in two university classrooms with natural ventilation in the Continental area of Spain. The research methodology was based on site sensors to analyze IAQ parameters such as CO2, Total Volatile Organic Compounds (TVOC), Particulate Matter (PM), and other climate parameters for a week during the cold season. Airtightness was then assessed within the classrooms and the close built environment by means of pressurization tests, and infiltration rates were estimated. The obtained results were used to set up a Computational Fluid Dynamics (CFD) model to evaluate the age of the local air and the ventilation efficiency value. The results revealed that ventilation cannot rely only on air infiltration, and, therefore, specific controlled ventilation strategies should be implemented to improve IAQ and to avoid excessive energy loss.

scholarly journals Sustainable Streetscape and Built Environment Designs around BRT Stations: A Stated Choice Experiment Using 3D Visualizations

2021 ◽  
Vol 13 (12) ◽  
pp. 6594
Author(s):  
Ahmad Adeel ◽  
Bruno Notteboom ◽  
Ansar Yasar ◽  
Kris Scheerlinck ◽  
Jeroen Stevens
Keyword(s):  
Built Environment ◽  
Mass Transit ◽  
Local Context ◽  
Transit Oriented Development ◽  
Commercial Building ◽  
Related Factors ◽  
Housing Projects ◽  
The Road ◽  
Development Goals ◽  
Set Up

The incompatibility between the microscale-built environment designs around mass transit stations and stakeholders’ preferences causes dissatisfaction and inconvenience. The lack of a pedestrian-friendly environment, uncontrolled development patterns, traffic and parking issues make the street life vulnerable and unattractive for users, and affect the mass transit usage. How to design the streetscapes around mass transit stations to provide a user-friendly street environment is a crucial question to achieve sustainable transit-oriented development goals. To recognize the specific attributes of streetscape environment relevant in local context of BRT Lahore, this paper presents the results of a visual preference experiment in which nine attributes of built environment were systematically varied across choice sets. Multinomial logit models were set up to identify the preferences of three target groups: BRT users, commercial building users and residents at different locations. The research indicates that not only the road-related factors (bike lane and sidewalk widths, crossings facilities, street greenery) have a significant influence on people’s preference but also that building heights, and the typology of buildings and housing projects around BRT corridor have shaped these preferences. When planning and designing urban design projects around mass transit projects, these significant attributes should be considered.

scholarly journals The Impact of Intervention-Related Risk Factors on the Risk of Ventilator-Associated Pneumonia Is High in a Neurosurgical Intensive Care Unit

2020 ◽  
Vol 41 (S1) ◽  
pp. s407-s409
Author(s):  
Ksenia Ershova ◽  
Oleg Khomenko ◽  
Olga Ershova ◽  
Ivan Savin ◽  
Natalia Kurdumova ◽  
...  
Keyword(s):  
Risk Factors ◽  
Mechanical Ventilation ◽  
Negative Slope ◽  
Infection Prevention And Control ◽  
Ventilator Associated Pneumonia ◽  
Related Factors ◽  
Icu Patients ◽  
The Impact ◽  
Adf Test ◽  
Over Time

Background: Ventilator-associated pneumonia (VAP) represents the highest burden among all healthcare-associated infections (HAIs), with a particularly high rate in patients in neurosurgical ICUs. Numerous VAP risk factors have been identified to provide a basis for preventive measures. However, the impact of individual factors on the risk of VAP is unclear. The goal of this study was to evaluate the dynamics of various VAP risk factors given the continuously declining prevalence of VAP in our neurosurgical ICU. Methods: This prospective cohort unit-based study included neurosurgical patients who stayed in the ICU >48 consecutive hours in 2011 through 2018. The infection prevention and control (IPC) program was implemented in 2010 and underwent changes to adopt best practices over time. We used a 2008 CDC definition for VAP. The dynamics of VAP risk factors was considered a time series and was checked for stationarity using theAugmented Dickey-Fuller test (ADF) test. The data were censored when a risk factor was present during and after VAP episodes. Results: In total, 2,957 ICU patients were included in the study, 476 of whom had VAP. Average annual prevalence of VAP decreased from 15.8 per 100 ICU patients in 2011 to 9.5 per 100 ICU patients in 2018 (Welch t test P value = 7.7e-16). The fitted linear model showed negative slope (Fig. 1). During a study period we observed substantial changes in some risk factors and no changes in others. Namely, we detected a decrease in the use of anxiolytics and antibiotics, decreased days on mechanical ventilation, and a lower rate of intestinal dysfunction, all of which were nonstationary processes with a declining trend (ADF testP > .05) (Fig. 2). However, there were no changes over time in such factors as average age, comorbidity index, level of consciousness, gender, and proportion of patients with brain trauma (Fig. 2). Conclusions: Our evidence-based IPC program was effective in lowering the prevalence of VAP and demonstrated which individual measures contributed to this improvement. By following the dynamics of known VAP risk factors over time, we found that their association with declining VAP prevalence varies significantly. Intervention-related factors (ie, use of antibiotics, anxiolytics and mechanical ventilation, and a rate of intestinal dysfunction) demonstrated significant reduction, and patient-related factors (ie, age, sex, comorbidity, etc) remained unchanged. Thus, according to the discriminative model, the intervention-related factors contributed more to the overall risk of VAP than did patient-related factors, and their reduction was associated with a decrease in VAP prevalence in our neurosurgical ICU.Funding: NoneDisclosures: None

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