scholarly journals Exposure Profile of SARS-CoV-2 in Canadian Food Sources.

2021 ◽  
Author(s):  
Megan Rose-Martel ◽  
Elizabeth Tompkins ◽  
Rebecca Rutley ◽  
Pablo Romero-Barrios ◽  
Enrico Buenaventura
Keyword(s):  
Food Packaging ◽  
Food Contamination ◽  
Food Sources ◽  
Animal Origin ◽  
Food Of Animal Origin ◽  
Contaminated Food ◽  
Exposure Profile ◽  
Human Digestive Tract ◽  
Exposure Routes ◽  
Foodborne Infection

A new coronavirus strain known as SARS-CoV-2 has spread throughout the world. This virus is the causative agent for the Coronavirus Disease 2019 (COVID-19) and spreads primarily through human-to-human transmission via infected droplets and aerosols generated by infected persons. While COVID-19 is a respiratory virus, the potential for transmission of SARS-CoV-2 via food is considered theoretically possible and remains a concern for Canadian consumers. We have conducted an exposure assessment of the likelihood of exposure of SARS-CoV-2 in Canadian food sources at the time of consumption. This article describes the exposure routes considered most relevant in the context of food contamination with SARS-CoV-2, including contaminated food of animal origin, other contaminated fresh foods, fomites and SARS-CoV-2 contaminated feces. The likelihood of foodborne infection of SARS-CoV-2 via the human digestive tract was also considered. Our analysis indicates that there is no evidence that foodborne transmission of SARS-CoV-2 has occurred and we consider the likelihood of contracting COVID-19 via food and food packaging in Canada as low to remote. Adherence to safe food practices and cleaning procedures would in any case prevent a potential foodborne infection with SARS-CoV-2.

scholarly journals Exposure Assessment of SARS-CoV-2 in Canadian Food Sources

2020 ◽  
Author(s):  
Megan Rose-Martel ◽  
Elizabeth Tompkins ◽  
Rebecca Rutley ◽  
Pablo Romero-Barrios ◽  
Enrico Buenaventura
Keyword(s):  
Exposure Assessment ◽  
Food Packaging ◽  
Food Contamination ◽  
Food Sources ◽  
Animal Origin ◽  
Food Of Animal Origin ◽  
Contaminated Food ◽  
Human Digestive Tract ◽  
Exposure Routes ◽  
Foodborne Infection

A new coronavirus strain known as SARS-CoV-2 has spread throughout the world. This virus is the causative agent for the Coronavirus Disease 2019 (COVID-19) and spreads primarily through human-to-human transmission via infected droplets and aerosols generated by infected persons. While COVID-19 is a respiratory virus, the potential for transmission of SARS-CoV-2 via food is considered theoretically possible and remains a concern for Canadian consumers. We have conducted a rapid exposure assessment of the likelihood of exposure of SARS-CoV-2 in Canadian food sources at the time of consumption. This article describes the exposure routes considered most relevant in the context of food contamination with SARS-CoV-2 in Canada, including contaminated food of animal origin, contaminated fresh foods, fomites and SARS-CoV-2 contaminated feces. The likelihood of foodborne infection of SARS-CoV-2 via the human digestive tract is also considered. Our analysis indicates that there is no indication of foodborne transmission of SARS-CoV-2 in Canadian food sources and we consider the concern of contracting COVID-19 via food and food packaging in Canada as low to remote. Adherence to safe food practices and disinfection procedures, including the thorough cooking of food, would in any case prevent a potential foodborne infection with SARS-CoV-2.

scholarly journals Determination of Cyclopropane Fatty Acids in Food of Animal Origin by 1H NMR

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Veronica Lolli ◽  
Angela Marseglia ◽  
Gerardo Palla ◽  
Emanuela Zanardi ◽  
Augusta Caligiani
Keyword(s):  
Fatty Acids ◽  
Dairy Products ◽  
Cyclopropane Ring ◽  
Food Sources ◽  
Animal Origin ◽  
Animal Fats ◽  
H Nmr ◽  
Cyclopropane Fatty Acids ◽  
Food Of Animal Origin ◽  
Full Scan

Cyclopropane fatty acids (CPFAs) are unusual fatty acids of microbial origin, recently detected in milk and dairy products. CPFAs have been demonstrated to be interesting molecular markers for authentication of dairy products obtained without ensiled feeds. Moreover, they can also be recognized as a new secondary component of human diet. Information is lacking on the presence of cyclic fatty acids in other food sources. Cyclopropane fatty acids have been detected by GC-MS analysis in cheese and other animal fats in concentration ranging from 200 to 1000 mg/kg fat, but in some cases, the complex fatty acid profile and the possible presence of interfering peaks make the separation not straightforward and the quantification uneasy. Therefore, a new reliable 1H NMR method was developed to detect and measure CPFA content in different foods of animal origin, based on the detection of the characteristic signals of cyclopropane ring. The 1H NMR (600 MHz) method showed detection limits comparable with those of full scan GC-MS, and it allowed the identification and quantitation of the cyclopropane fatty acids in different foods.

scholarly journals A Proof of Principle for the Detection of Viable Brucella spp. in Raw Milk by qPCR Targeting Bacteriophages

2020 ◽  
Vol 8 (9) ◽  
pp. 1326
Author(s):  
Michaela Projahn ◽  
Jens A. Hammerl ◽  
Ralf Dieckmann ◽  
Sascha Al Dahouk
Keyword(s):  
Limit Of Detection ◽  
Raw Milk ◽  
Qpcr Assay ◽  
Animal Origin ◽  
Indicator Organisms ◽  
Food Of Animal Origin ◽  
Global Health Issue ◽  
Contaminated Food ◽  
Viable Bacteria ◽  
And Performance

Brucellosis is still a global health issue, and surveillance and control of this zoonotic disease in livestock remains a challenge. Human outbreaks are mainly linked to the consumption of unpasteurized dairy products. The detection of human pathogenic Brucella species in food of animal origin is time-consuming and laborious. Bacteriophages are broadly applied to the typing of Brucella isolates from pure culture. Since phages intracellularly replicate to very high numbers, they can also be used as specific indicator organisms of their host bacteria. We developed a novel real-time PCR (qPCR) assay targeting the highly conserved helicase sequence harbored in all currently known Brucella-specific lytic phages. Quality and performance tests determined a limit of detection of <1 genomic copy/µL. In raw milk artificially contaminated with Brucella microti, Izv phages were reliably detected after 39 h of incubation, indicating the presence of viable bacteria. The qPCR assay showed high stability in the milk matrix and significantly shortened the time to diagnosis when compared to traditional culture-based techniques. Hence, our molecular assay is a reliable and sensitive method to analyze phage titers, may help to reduce the hands-on time needed for the screening of potentially contaminated food, and reveals infection risks without bacterial isolation.

scholarly journals Resistance to antibiotics and genotype characteristics of Salmonella enterica subspecies enterica serovar Mbandaka isolated from poultry

2008 ◽  
Vol 62 (5-6) ◽  
pp. 351-358
Author(s):  
Dejan Vidanovic ◽  
Z. Sabo ◽  
Natasa Kilibarda ◽  
Mira Zivadinovic ◽  
Aleksandar Zarkovic ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Research Work ◽  
Animal Origin ◽  
Antibiotics Resistance ◽  
Resistance To Antibiotics ◽  
Food Of Animal Origin ◽  
Geographical Regions ◽  
Contaminated Food ◽  
Degree Of Certainty ◽  
Multiresistant Strains

Salmonellas are one of the main zoonotic pathogens whose reservoirs are poultry, cattle and pigs. By means of the food chain salmonellas can be transferred to humans through contaminated food of animal origin. Multiresistant strains Salmonella are particularly dangerous since they can transfer genes of resistance to antibiotics to other microorganisms. Control of salmonellas primarily depends on a good surveillance system and knowledge of the strain types present in the epizootiologic area. In some geographical regions only a few Salmonella serotypes are usually of epidemiological importance. Due to the predomination of some serotypes and fagotypes, when an additional discrimination within serotypes and fagotypes is needed, DNA genotyping is used. In cases when it is necessary to compare the strains which caused the poisoning of patients, with strains isolated from food or animals, a highly discriminatory method is used - pulsed field gel electrophoresis (PFGE). Due to a high degree of discrimination the results of PFGE testing enable decision making with a higher degree of certainty in epizootiologic and epidemiologic research work. The aim of this testing was to determine the antibiotics resistance and genotype characteristics of Salmonella enterica subsp. enterica serovar Mbandaka isolated from poultry from some areas of the territory of Serbia.

scholarly journals Impact of hygienic slaughter practices on Salmonella contamination of broiler carcasses in Biskra, Algeria

2020 ◽  
Vol 51 (4) ◽  
pp. 463-470
Author(s):  
Nadjah Guergueb ◽  
Laila Aoun ◽  
Omar Bennoun ◽  
Ammar Ayachi ◽  
Ilhem Chachoua ◽  
...  
Keyword(s):  
Risk Factors ◽  
Poultry Meat ◽  
World Health ◽  
Animal Origin ◽  
Broiler Meat ◽  
Food Of Animal Origin ◽  
Contaminated Food ◽  
Health Organization ◽  
Live Bird

Poultry meat is the primary meat consumed in Algeria, surpassing sheep and beef in the 1980s. However, this product is often at risk of being contaminated by Salmonella. Salmonellosis is a foodborne disease with tens of millions of human cases estimated to occur worldwide, and resulting in more than a hundred thousand deaths per year. According to the World Health Organization, salmonellosis in humans is generally contracted through the consumption of contaminated food of animal origin, including poultry. The main objective of this study was to investigate the risk factors associated with Salmonella contamination of broiler carcasses at the slaughterhouse. Sixty fresh chicken carcasses from six slaughterhouses were subjected to bacteriological analysis in accordance with AFNOR (French Standardization Association) standards. Statistical analyses showed an impact of the hygienic quality of the slaughter process on the likelihood of Salmonella contamination of poultry meat. A correlation (r=0.84) was found between hygienic slaughter practices and Salmonella contamination of chicken carcasses. The average level of TAMB (total aerobic mesophilic bacteria) was high in carcasses positive for Salmonella (t-test = 0.019). Not washing live bird transport crates was associated with an increasing risk of Salmonella contamination carcasses (odds ratio/OR = 28). The mean level of TAMB was higher in the presence of the following risk factors: old and small slaughterhouse, not washing live bird transport crates, manual bleeding, scalding type (soaking), non-renewal of scalding water, no disinfectant in water, no ventilation drying, no sanitary facilities. The identification of risk factors responsible for bacterial contamination of broiler meat is essential to determine the most effective methods of prevention.

scholarly journals Emerging Porcine adenovirus PAdV-SVN1 and other enteric viruses in samples of industrialized meat by-products

2020 ◽  
Vol 50 (12) ◽  
Author(s):  
Fernanda Gil de Souza ◽  
Artur Fogaça Lima ◽  
Viviane Girardi ◽  
Thalles Guillem Machado ◽  
Victória Brandalise ◽  
...  
Keyword(s):  
Enteric Viruses ◽  
Human Adenovirus ◽  
Hepatitis E ◽  
Animal Origin ◽  
Food Of Animal Origin ◽  
Porcine Adenovirus ◽  
Contaminated Food ◽  
New Type ◽  
First Time ◽  
By Products

ABSTRACT: Foodborne diseases are often related to consumption of contaminated food or water. Viral agents are important sources of contamination and frequently reported in food of animal origin. The goal of this study was to detect emerging enteric viruses in samples of industrialized foods of animal origin collected in establishments from southern of Brazil. In the analyzed samples, no Hepatitis E virus (HEV) genome was detected. However, 21.8% (21/96) of the samples were positive for Rotavirus (RVA) and 61.4% (59/96) for Adenovirus (AdV), including Human adenovirus-C (HAdV-C), Porcine adenovirus-3 (PAdV-3) and new type of porcine adenovirus PAdV-SVN1. In the present research, PAdV-SVN1 was detected in foods for the first time. The presence of these viruses may be related to poor hygiene in sites of food preparation, production or during handling.

scholarly journals Dietary Intake, Nutritional Adequacy, and Food Sources of Protein and Relationships with Personal and Family Factors in Spanish Children Aged One to < 10 Years: Findings of the EsNuPI Study

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1062
Author(s):  
Casandra Madrigal ◽  
María José Soto-Méndez ◽  
Ángela Hernández-Ruiz ◽  
Teresa Valero ◽  
Federico Lara Villoslada ◽  
...  
Keyword(s):  
Dietary Protein ◽  
Total Protein ◽  
Dairy Products ◽  
Protein Intake ◽  
Meat Products ◽  
Food Sources ◽  
Animal Origin ◽  
Total Protein Intake ◽  
Fish And Shellfish ◽  
Milk And Dairy Products

Diet in the first years of life is an important factor in growth and development. Dietary protein is a critical macronutrient that provides both essential and nonessential amino acids required for sustaining all body functions and procedures, providing the structural basis to maintain life and healthy development and growth in children. In this study, our aim was to describe the total protein intake, type and food sources of protein, the adequacy to the Population Reference Intake (PRI) for protein by the European Food Safety Authority (EFSA), and the Recommended Dietary Allowance (RDA) by the Institute of Medicine (IoM). Furthermore, we analyzed whether the consumption of dairy products (including regular milk, dairy products, or adapted milk formulas) is associated with nutrient adequacy and the contribution of protein to diet and whole dietary profile in the two cohorts of the EsNuPI (in English, Nutritional Study in the Spanish Pediatric Population) study; one cohort was representative of the Spanish population from one to < 10 years old (n = 707) (Spanish reference cohort, SRS) who reported consuming all kinds of milk and one was a cohort of the same age who reported consuming adapted milk over the last year (including follow-on formula, growing up milk, toddler’s milk, and enriched and fortified milks) (n = 741) (adapted milk consumers cohort, AMS). The children of both cohorts had a high contribution from protein to total energy intake (16.79% SRS and 15.63% AMS) and a high total protein intake (60.89 g/day SRS and 53.43 g/day AMS). We observed that protein intake in Spanish children aged one to < 10 years old was above the European and international recommendations, as well as the recommended percentages for energy intakes. The main protein sources were milk and dairy products (28% SRS and 29% AMS) and meat and meat products (27% SRS and 26% AMS), followed by cereals (16% SRS and 15% AMS), fish and shellfish (8% in both cohorts), eggs (5% SRS and 6% AMS), and legumes (4% in both cohorts). In our study population, protein intake was mainly from an animal origin (meat and meat products, milk and dairy products, fish and shellfish, and eggs) rather than from a plant origin (cereals and legumes). Future studies should investigate the long-term effect of dietary protein in early childhood on growth and body composition, and whether high protein intake affects health later in life.

scholarly journals Presence of Perfluoroalkyl and Polyfluoroalkyl Substances (PFAS) In Food Contact Materials (FCM) and Its Migration to Food

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1443
Author(s):  
Arabela Ramírez Carnero ◽  
Antía Lestido-Cardama ◽  
Patricia Vazquez Loureiro ◽  
Letricia Barbosa-Pereira ◽  
Ana Rodríguez Bernaldo de Quirós ◽  
...  
Keyword(s):  
Fast Food ◽  
Food Packaging ◽  
Health Concern ◽  
Contact Materials ◽  
Food Contact Materials ◽  
Food Contact ◽  
Contaminated Food ◽  
Polyfluoroalkyl Substances ◽  
And Storage ◽  
Perfluoroalkyl And Polyfluoroalkyl Substances

Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are synthetic chemical compounds widely used in different industry fields including food contact materials (FCM), providing resistance to fat and humidity, and non-stick properties. PFAS enter into the food chain directly from the intake of contaminated food or indirectly from the migration of the FCM into the food. This exposure published in different research highlights a public health concern. Therefore, it is necessary to perform analysis of the content of different FCM and evaluate the migration from the FCM under normal conditions of use and storage. This bibliographical review proves that different perfluoroalkyl and polyfluoroalkyl compounds are detected in fast food packaging, microwave popcorn bags, and frying pans, among others. Furthermore, it shows the conditions or factors that favor the migration of the PFAS from the FCM into the food.

scholarly journals Undeclared (Poly)phosphates Detection in Food of Animal Origin as a Potential Tool toward Fraud Prevention

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1547
Author(s):  
Radmila Pavlovic ◽  
Federica Di Cesare ◽  
Francesca Longo ◽  
Franco Abballe ◽  
Sara Panseri ◽  
...  
Keyword(s):  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Exchange Chromatography ◽  
Animal Origin ◽  
Food Of Animal Origin ◽  
Conductometric Detection ◽  
Q Exactive ◽  
Phosphate Treatment ◽  
Potential Tool ◽  
Vulnerable Population Groups

(Poly)phosphates are approved as water-preserving and emulsifying agents that improve the appearance and consistency of many food products. The labelling of added (poly)phosphates is essential for protecting vulnerable population groups and to prevent unfair trade practices resulting in economic fraud. The problems with (poly)phosphates’ utilisation concerns both analytical and legislative issues, such as: (1) their straightforward detection; (2) excessive addition altering freshness perception and misleading consumers; (3) uncontrolled usage increasing foodstuff weight; (4) application in products where they are not permitted; and (5) no indication on the label. Bearing all these issues in mind, the main purpose of this study was the quantification and screening of the (poly)phosphates profile in meat, marine and dairy products (160 samples), of which 43 were without declared (poly)phosphate treatment. Analysis was completed by high-performance ion-exchange chromatography either with conductometric detection or coupled to Q-Exactive Orbitrap high-resolution mass spectrometry. Although the (poly)phosphates profiles varied greatly according to species and processing type, the following criteria for detection of illicit treatment were established: high orthophosphate level, quantified short-chain (poly)phosphate anions and the presence of long-chain forms. In conclusion, the instrumental platforms used in this study can be recommended to inspection bodies as reliable methods for the detection of food adulteration with (poly)phosphates.

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