scholarly journals Gut Histomorphometric and Blood Profile of Broiler Chicks Fed Varied Levels Of Protein

Author(s):  
Shittu, M. D ◽  
Adejumo, D. O, ◽  
Ademola, S. G, ◽  
Alagbe, J.O

Gut plays active role in screening what goes into the blood stream and blood is very important parameter to access for us to establish the health status of animal to any dietary variation, this will help the producer ability to guaranty the consumers’ health. Therefore this research was designed to determine gut histomorphometry and blood profile of broiler starter fed varied levels of protein. Material and method is A total of 144 days-old-broiler chicks were used for the study. Broiler standard starter mash with varied protein percentages of 20, 23, and 26%, respectively for treatments 1, 2 and 3 were used. Data were analysed using ANOVA at α0.05. Results is Except crude protein and ash contents, others nutrient digestibilities were not affected by the dietary treatments. Birds fed lower crude protein had the lowest crude protein digestibility while birds fed standard diet (23% CP) had the best and highest crude protein digestibility. White blood cell decrease with decrease dietary protein, this might have serious effect on the immunity level of the broilers. Also globulin increases while albumin decreases with increase in dietary protein levels. Serum total protein increases with increase in the dietary protein levels and diet with26% CP recorded the highest value (5.82g/dL). Serum cholesterol values were significantly higher at lower protein level unlike serum protein whiledifferent gut parts response significantly different to dietary treatments. Conclusion is at early stage broiler growth, diet need to have higher CP to improve the immunity and health growth of gut.

1977 ◽  
Vol 24 (1) ◽  
pp. 69-75 ◽  
Author(s):  
R. G. Campbell

SUMMARYThirty-six male piglets weaned at 20 days of age were allocated to an initial slaughter group and to five dietary treatments with proteinlevels of 15·0, 17·2, 19·1, 21·4 and 23·2% crude protein at a common energy level of approximately 3·6 Meal digestible energy/kg. All diets were offered ad libitum and growth response was compared between 5 and 20 kg and 10 and 20 kg live weight. In both periods there were significant improvements in growth and the food conversion ratio from 15·0 to 17·2%, and 17·2 to 19·1% crude protein. Between 10 and 20 kg live weight dietary protein levels above 19·1% were associated with a depression in growth. Carcass protein increased and ether-extractable material decreased with increased dietary protein over the entire range studied. Retention of digestible nitrogen was maximized on the lower protein diets. Conversion of food into carcass lean was maximized on the two highest protein diets.


2005 ◽  
Vol 75 (5) ◽  
pp. 357-368 ◽  
Author(s):  
Foote ◽  
Nonnecke† ◽  
Waters ◽  
Palmer ◽  
Beitz ◽  
...  

Effects of increased protein and energy provided by an intensified milk replacer on the antigen-specific, cell-mediated immune response of the neonatal calf were examined. Calves were fed a standard (0.45 kg/day of a 20% crude protein, 20% fat milk replacer; n = 11) or intensified (1.14 kg/day of a 28% crude protein, 20% fat milk replacer; n = 11) diet from 0 to 6 weeks of age. All calves were vaccinated with Mycobacterium bovis bacillus Calmette-Guerin (BCG) at 1 week of age. The daily weight gain of intensified-diet calves (0.62 kg/day) was greater than the weight gain of standard-diet calves (0.29 kg/day). Liver, kidney, heart, thymus, and subcervical lymph nodes from intensified-diet calves were heavier than the same organs from standard-diet calves. Flow cytometric analysis of peripheral blood mononuclear cell (PBMC) populations indicated that CD4+ cells, gamma delta TCR+ cells, and monocyte percentages, although unaffected by diet during the first 5 weeks of the study, were higher in intensified-diet calves at week 6. The decline in gamma deltad TCR+ cell percentages and increase in B cell percentages with increasing age seen in all calves are characteristic of the maturing immune system of the calf. CD8+ T cell or B cell percentages were not affected by diet. In intensified-diet calves, percentages of CD4+ expressing interleukin-2 receptor increased and percentages of gamma delta TCR+ cells expressing interleukin-2 receptor decreased with time. The same populations in standard-diet calves did not change with time. Percentages of CD4+ and CD8+ T cells, and B cells expressing MHC class II antigen, were unaffected by diet or age. Although mitogen-induced interferon (IFN)-gamma and nitric oxide (NO) secretion increased with age for all calves, PBMC from intensified-diet calves produced less IFN-gamma and more NO than did cells from standard-diet calves at week 6 of the study. Antigen-induced secretion of IFN-gamma and NO also increased with age but was unaffected by diet. Antigen-elicited delayed-type hypersensitivity was unaffected by diet, suggesting increased dietary protein and energy did not alter adaptive immunity in vivo. Overall, these results suggest that feeding calves a commercially available, intensified milk replacer affects minimally the composition and functional capacities of PBMC populations. Additional research is necessary to determine whether these subtle effects influence the calf’s susceptibility to infectious disease.


2013 ◽  
Vol 48 (6) ◽  
pp. 645-650 ◽  
Author(s):  
Cleujosí da Silva Nunes ◽  
Gilberto Moraes ◽  
Fernando Fabrizzi ◽  
Araceli Hackbarth ◽  
Gustavo Alberto Arbeláez-Rojas

The objective of this work was to evaluate the effect of sustained swimming and dietary protein levels on growth and hematological responses of juvenile pacu (Piaractus mesopotamicus). A completely randomized design was used in a 3x2 factorial arrangement, with three levels of dietary protein (24, 28, and 32% crude protein), two rearing conditions (sustained swimming or motionless water), and 15 replicates. Fish were subjected to sustained swimming at the velocity of two body lengths per second (2 BL s-1), for 45 days. The level of dietary protein and the swimming conditions affected the performance, growth, and hematological profile of pacu. Swimming conditions influenced nutritional factors, increasing daily weight gain, specific growth rate, number of erythrocytes, mean corpuscular volume, and mean corpuscular hemoglobin. Fish under sustained swimming and fed with 24% crude protein showed better growth performance, with higher specific growth rate (4.11±0.88) and higher daily weight gain (2.19±0.47 g per day). Sustained swimming can increase the productive performance of pacu and simultaneously reduce dietary protein levels.


2021 ◽  
Vol 4 (1) ◽  
pp. 239-253
Author(s):  
M. J. Olomu

Three experiments were conducted to determine the optimum protein and energy levels for finishing broiler chickens in a tropical environment. Dietary protein levels (20, 23 and 26 percent) and energy levels (2800, 3000 and 3200 Kcal/kg diet) tested had no significant effects on performance of finishing chickens fed fishmeal-containing rations. Rations based on cereals and groundnut meal, without fishmeal, did not support maximum liveweight gain and feed efficiency at dietary protein levels below 26 percent. Dietary treatments had no significant effects on carcass dressing percentages. Based on the present results, a protein level of 20 percent(for fishmeal containing rations) and 23 or 26 percent (for rations based on groundnut meal without fishmeal) and energy levels of 2800 to 3000 Kcal/Kg diet are tentatively recommended for finishing broiler chickens raised in a tropical country like Nigeria.


2017 ◽  
Vol 25 (3) ◽  
pp. 157-164 ◽  
Author(s):  
Muhammet Altunok ◽  
Osman Özden

Abstract The purpose of the study was to obtain preliminary data on the effect of dietary protein on the growth of the thick-lipped grey mullet (Chelon labrosus) juveniles reared in net sea-cages. The juveniles (6.2 ± 0.61 g) were obtained from the wild and were stocked into net sea-cages at a stocking rate of 100 juveniles per cubic meter and fed with experimental diets including 25, 30, or 35% crude protein for 87 days. The highest mean weight and specific growth rate (SGR) were 39.5 g and 2.13% day−1, respectively, at protein levels of 30%. The diet also resulted in the most efficient feed conversion ratio of 1.61.


2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Dong Wang ◽  
Guoshun Chen ◽  
Lili Song ◽  
Mingjie Chai ◽  
Yongfeng Wang ◽  
...  

Diets containing different crude protein levels (16%, 14%, and 12%) were created to feed Bamei pigs in order to study the effect of these compositions on intestinal colonies. Therefore, 27 healthy Bamei pigs of similar weight ( 20.99   kg ± 0.16   kg ) were selected and randomly divided into three groups for microbial diversity analysis. The results of this study show that microbial diversities and abundances in Bamei pig jejunum and caecum samples after feeding with different dietary protein levels were significantly different. Dietary crude protein level exerted no significant effect on the Shannon index for cecum microbes in these pigs, while Simpson, ACE, and Chao1 indices for group I were all significantly higher than those of either the control group or group II ( P < 0.05 ). Indeed, data show that microbial diversities and abundances in the 14% protein level group were higher than those in either the 16% or 12% groups. Dominant bacteria present in jejunum and cecum samples given low-protein diets were members of the phyla Firmicutes and Bacteroidetes. Data show that as dietary crude protein level decreases, representatives of the microbial flora genus Lactobacillus in jejunum and cecum samples gradually increases. Values for the KEGG functional prediction of microbial flora at different dietary protein levels also show that genes of jejunum and cecum microorganisms were mainly enriched in the “metabolism” pathway and indicate that low protein diets increase intestinal metabolic activity. Therefore, we recommend that Bamei pig dietary protein levels are reduced 2% from their existing level of 16% crude protein. We also suggest that essential synthetic amino acids (AA) are added to optimize this ideal protein model as this will increase intestinal flora diversity in these pigs and enhance health. These changes will have a positive effect in promoting the healthy growth of Bamei pigs.


2021 ◽  
Vol 12 ◽  
Author(s):  
Xin Tao ◽  
Bo Deng ◽  
Qizhi Yuan ◽  
Xiaoming Men ◽  
Jie Wu ◽  
...  

Low protein diets are commonly used in the growing-finishing pig stage of swine production; however, the effects of low dietary protein on the intestinal microbiota and their metabolites, and their association with pig sex, remain unclear. The present study aimed to assess the impact of a low crude protein (CP) diet on the gut microbiome and metabolome, and to reveal any relationship with sex. Barrows and gilts (both n = 24; initial body = 68.33 ± 0.881 kg) were allocated into two treatments according to sex. The four groups comprised two pairs of gilts and barrows fed with a high protein diet (CP 17% at stage I; CP 13% at stage II) and a low protein diet (CP 15% at stage I; CP 11% at stage II), respectively, for 51 d. Eight pigs in each group were slaughtered and their colon contents were collected. Intestinal microbiota and their metabolites were assessed using 16S rRNA sequencing and tandem mass spectrometry, respectively. The low protein diet increased intestinal microbiota species and richness indices (P &lt; 0.05) in both sexes compared with the high protein diet. The sample Shannon index was different (P &lt; 0.01) between barrows and gilts. At the genus level, unidentified Clostridiales (P &lt; 0.05), Neisseria (P &lt; 0.05), unidentified Prevotellaceae (P &lt; 0.01) and Gracilibacteria (P &lt; 0.05) were affected by dietary protein levels. The relative abundance of unidentified Prevotellaceae was different (P &lt; 0.01) between barrows and gilts. The influence of dietary protein levels on Neisseria (P &lt; 0.05), unidentified Prevotellaceae (P &lt; 0.01) and Gracilibacteria (P &lt; 0.05) were associated with sex. Metabolomic profiling indicated that dietary protein levels mainly affected intestinal metabolites in gilts rather than barrows. A total of 434 differentially abundant metabolites were identified in gilts fed the two protein diets. Correlation analysis identified that six differentially abundant microbiota communities were closely associated with twelve metabolites that were enriched for amino acids, inflammation, immune, and disease-related metabolic pathways. These results suggested that decreasing dietary protein contents changed the intestinal microbiota in growing-finishing pigs, which selectively affected the intestinal metabolite profiles in gilts.


2020 ◽  
Vol 53 (3) ◽  
pp. 117-125
Author(s):  
Taiwo Kamar-deen Bello ◽  
Peter Ibrahim Rekwot ◽  
Joseph Olusegun Ayo ◽  
Balkisu Banke Oyelowo ◽  
Armiyau Mada Khumran ◽  
...  

AbstractThe increasing demand of the feed market for protein has necessitated the need to look for and use other sources of this nutrient. Cottonseed cake and palm kernel cakes are used extensively for supplementing ruminant rations to increase their productivity. The efficiency of sperm production, libido and quality of spermatozoa tend to remain uniform throughout the reproductive life of an animal but may be significantly altered by nutrition. The aim of this study was to assess the effects of graded levels of protein on semen characteristics, and serum testosterone profile in Yankasa rams. Fifteen rams aged between 18–24 months and weighing 15–25 kg with good body condition scores (3.5) were used in this study. They were divided into three treatment groups (A, B and C) according to the dietary protein level. Group A (n = 5) received 10 %, group B (n = 5) received 15 % while group C (n = 5) received 20 % combined crude protein of cotton seed and palm kernel cakes, respectively. Semen samples were collected weekly using battery-controlled electro ejaculator. Serum samples were harvested using a Pasteur pipette into serum vials and stored at −20 °C for analysis. These samples were from a representative animal in each group for determination of testosterone profiles using ELISA technique at weeks 1, 6 and 12. Data collected were expressed as means and standard error of the mean (± SEM). Significance of differences between treatments means were estimated at P ≤ 0.05 with Tukey-Kramer multiple comparison test of repeated measures analysis of variance (ANOVA). From this study, it was concluded that rams fed concentrate of 15 % crude protein using cotton seed and palm kernel cakes combined had improved semen characteristics in terms of semen concentration, motility, live sperm cells and morphology, but the crude protein levels had no effects on testosterone concentrations.


2007 ◽  
Vol 23 (5-6-2) ◽  
pp. 339-346
Author(s):  
Z. Pavlovski ◽  
N. Nikolova ◽  
D. Vitorovic ◽  
Z. Skrbic ◽  
M. Lukic

Cobb 500 and Hubbard Classic broiler chicks were reared on the floor during seven weeks of fattening. Chicks, of each strain, were divided in two groups (160 chicks in group) and feed with two isocaloric diets differed in protein levels. The contents of crude protein in the diets 1, were 23 % (1-2 weeks of age), 22 % (3. week), 21 % (4 - 6 weeks), 18 % (7. week) and in the diets 2 were 22 % (1 - 2 weeks), 21 % (3. week), 20 % (4 - 6 weeks) and 17 % (7. week of age). At days 35, 42 and 49, 10 male and 10 female chicks were sacrificed from both strain and both group. The right tibiae were removed and prepared for analysis. Bone breaking force were determined by three-point-bending test at the midlength. Geometrical properties were obtained by measuring of outside and inside antero-posterior and lateromedial diameters, at the middiaphyseal transverse section of each tibia. These data were used to calculated of bones cross sectional cortical area and cross sectional medullar area. The obtained results showed that cross sectional cortical area and cross sectional medullar area increased with age and were influenced by strain and sex but not by dietary protein levels. Hubbard Classic chicks had higher cortical and lower medullar area than Cobb 500 chicks. The breaking force and ratio cortical/medullar area were significantly (p< 0.05) greater in male than in female chicks of each strain. The male chicks of both strain, fed the diets 2, during six weeks of fattening, had the largest tibiae breaking force (47.0-50.0 kg) but the differences were not significant. Geometrical and mechanical characteristics of broilers tibiae were notsignificantly influenced by dietary protein levels.


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