scholarly journals Heritabilitas Produki Telur Ayam Lokal Papua Berbeda Genotip dari Lokus 24- bp Insertion-Deletion dalam Promotor Gen Prolaktin

Author(s):  
Muhammad Affan Mu'in ◽  
Sintje Lumatauw

The heritability (h2) of a trait shows phenotypes variance of the trait caused by additive genetic variance. The h2 value is used to estimate the quantitative trait breeding value of livestock in order to improve these traits through selection. This study aims to estimate the h2 of egg production characteristics in Papua local chickens with different genotypes from the 24-bp InDel (Insertion-Deletion) locus in the prolactin gene promoter region (24-bp InDel/cPRLp locus). A total of 13 pairs of Papua local chickens consisting of 3 pairs of II genotypes (♂II x ♀II) and 11 female offspring, 5 pairs of ID genotypes (♂ID x ♂ID) and 19 female offspring, and 5 pairs of DD genotypes (DD x ♀DD) and 17 female offspring were used in this study. Observations were made on the characteristics of egg production in female offspring of each genotype group. The variance component for h2 estimation is obtained by the one-way analysis of variance method and the separation of the variance components for single pairs. The results showed that the h2 at first laying of eggs in all genotype groups was moderate (0.10 to 0.30); the h2 of the number of eggs produced from the time they first laid eggs until the age of 240 days in II and ID genotype groups was high (> 0.3), while in the DD genotype group was classified as moderate (0.10 to 0.30); and the h2 of egg weight in all genotype groups was moderate (0.10 to 0.30). The high h2 of a trait indicates that the trait is more dominated by additive genes and is more responsive to the selection treatment.

2021 ◽  
pp. 18-26
Author(s):  
Muhammad Affan Mu'in ◽  
Sintje Lumatauw

This study aims to estimate the response of the number of eggs produced up to 240 days of age (EN240) to the selection of Papua local chickens (PLC) with different genotypes from the 24-bp Indel cPRLp locus. A total of 68 PLCs were taken randomly from several breeders in Manokwari as the basic population for selection (G0). Genotype identification of the 24-bp Indel cPRLp locus was performed G0. Based on the distribution of these genotypes, mating pairs were randomly formed G0-II, G0-DD and G0-ID to produce G1-II, G1-DD, and G1-Control (G0-ID offspring). Heritability of EN240 in G1-II and G1-DD populations were estimated in full-sib (single pairs mating). Individual selection based on breeding value EN240 was carried out on G1-II (♀) and G1-DD (♀) to form the selection generation (GS): GS-II (♀) and GS-DD (♀). Selection was also carried out on G1-II (♂) and G1-DD (♂) based on body weight at 240 days (BW240) to become selected GS-II (♀) and GS-DD (♀) mating partners. GS-Control was also formed through 25% random sampling from G1-Control (♂ and ♀). GS mating resulted in the second generation (G2): G2-II, G2-DD, and G2-Control. Age at first laying (AFL), EN240, and mean egg weight (EWA) in hens of G1, GS, and G2 were recorded. Response to selection for EN240 was calculated by two methods predicted selection response (Rp) and actual selection response (Rr). Both methods of calculation yield positive and high values. In actual response (Rr), PLC in II genotypes group are more responsive to the selection treatment than DD genotype group. Selection increase EN240 impact accelerate of AFL and lower the EWA, because of their negative genetic correlation.


2019 ◽  
Vol 51 (1) ◽  
Author(s):  
Evert W. Brascamp ◽  
Piter Bijma

Abstract Background In honey bees, observations are usually made on colonies. The phenotype of a colony is affected by the average breeding value for the worker effect of the thousands of workers in the colony (the worker group) and by the breeding value for the queen effect of the queen of the colony. Because the worker group consists of multiple individuals, interpretation of the variance components and heritabilities of phenotypes observed on the colony and of the accuracy of selection is not straightforward. The additive genetic variance among worker groups depends on the additive genetic relationship between the drone-producing queens (DPQ) that produce the drones that mate with the queen. Results Here, we clarify how the relatedness between DPQ affects phenotypic variance, heritability and accuracy of the estimated breeding values of replacement queens. Second, we use simulation to investigate the effect of assumptions about the relatedness between DPQ in the base population on estimates of genetic parameters. Relatedness between DPQ in the base generation may differ considerably between populations because of their history. Conclusions Our results show that estimates of (co)variance components and derived genetic parameters were seriously biased (25% too high or too low) when assumptions on the relationship between DPQ in the statistical analysis did not agree with reality.


1975 ◽  
Vol 55 (4) ◽  
pp. 741-747 ◽  
Author(s):  
J. H. STRAIN ◽  
A. P. PILOSKI

Dwarf broiler breeders in single-bird cages (DC), in floor pens (DF) and normal broiler breeders in floor pens (NF) were compared for their own and their offspring’s performance. No differences were observed in rate of egg production at 147–329 days of age between the NF and DF birds (48 vs. 47%) whereas the DC birds laid at the rate of 40%. Hen-housed egg numbers for the same period followed the same general pattern (NF, 81; DF, 82; DC, 70 eggs/bird). DF and DC birds consumed less feed per dozen eggs than the NF birds (3.4 vs. 4.3 kg/doz). Egg weights in the early part of the production cycle were not significantly different among the groups but at 294 days of age the normal birds laid larger eggs than the dwarfs (65 vs. 62 g). Dwarfs in cages reached 50% production earlier than the DF or NF birds. Female offspring from the three groups grew at the same rate and weighed 1.7 kg at 56 days of age but there were significant differences in feed conversion (NF, 2.46; DF, 2.43; DC, 2.53 kg/kg body weight produced). Male offspring had small but not statistically significant differences in body weight at 56 days (NF, 2.16; DF, 2.12; DC, 2.09 kg) or feed conversion (NF, 2.30; DF, 2.29; DC, 2.34 kg/kg body weight). Mortality in the offspring ranged from 2.7 to 9.2% but did not differ significantly. Income over feed costs per broiler started did not differ from the three groups regardless of the meat price – feed price combination considered. However, when profit was computed on a breeder hen basis, the DF females were more profitable than either the NF or DC females.


Author(s):  
Azhaguraja Manoharan ◽  
S. Sankaralingam ◽  
P. Anitha ◽  
Binoj Chacko ◽  
T.V. Aravindakshan

Background: Prolactin is a physiological candidate gene which has significant effects on egg production in poultry. Also, it plays a major role on incubation behaviour (broodiness) in birds. The main objective of the present study was identification of single nucleotide polymorphism (SNP) C-2161G in the promoter region of prolactin gene and its association with egg production in Tellicherry native chicken population. Methods: A total of 200 blood samples were collected from the randomly selected birds of Tellicherry native chicken in All India Co-ordinated Research Project on Poultry improvement (AICRP) farm, Mannuthy, Thrissur, Kerala. Isolation of Genomic DNA was done and the isolated samples were subjected to polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis to identify the SNP C-2161G of prolactin gene. Result: On the basis of RFLP patterns, birds were designated with three different genotypes namely CC, CG and GG. The allelic and genotypic frequency was calculated. The observed genotypic frequency at the SNP site C-2161G was CC (0.61), CG (0.160) and GG (0.23) and the frequency of allele was 0.69 for C and 0.31 for G. The egg production was shown to be statistically similar for the genotypes of SNP C-2161G.


Genetika ◽  
2014 ◽  
Vol 46 (2) ◽  
pp. 591-599 ◽  
Author(s):  
Mehrangiz Fathi ◽  
Ghorban Elyasi-Zarringhobaie

The induction and regulation of broodiness is of the most important role of prolactin in avian species. In this study, the association between prolactin promoter region alleles and reproductive traits in Fars native turkey was investigated. These traits consisted of mean egg weight (MEW), number of egg (EN) and egg mass, during the first laying period. In total, 115 laying turkeys, randomly selected from the flock of the Breeding Center for Fars Native turkey, and DNA was purificated from blood samples, 231 bp of prolactin promoter region was amplified and Genotype of Samples was determinate by PCR-SSCP technique were genotyped. Two alleles D and I were identified. Based on the results obtained, the frequency of D and I alleles were 0.67 and 0.33, respectively. Frequencies of DD, II and ID genotypes were 0.385, 0.044 and 0.571, respectively. The association analysis between the polymorphism PRL gene promoter region and egg performance was carried out. Significant relationship was found between genotypes with egg production (P<0.01). Individuals with II genotype produced higher egg production than DD and ID genotype. The results of current study showed that using information of genes related to egg production could be used to improve the performance of native turkey of East Azerbaijan province.


2021 ◽  
Vol 748 (1) ◽  
pp. 012003
Author(s):  
N.G.A Mulyantini S.S ◽  
Ulrikus R Lole

Abstract The objective of this study was to compare the performance of four-breed combination under semiintensive management system. The experiment used 160 females and 40 males chickens as parents. Artificial insemination was performed to produce the experimental chicken of the four breed combinations (1. Sabu x Sabu, 2. Sabu x Semau, 3. Sabu x KUB, 4. Semau x KUB). The chickens were fed with chick starter crumb sad libitum from day old to 4 weeks of age. From 4-8 weeks of age, chickens were allowed to scavenge during the day, and also given commercial grower pellets + rice bran + corn (5:4:1). Combination of Sabu x KUBfrom 4-8 weeks of age were significantly heavier (P < 0.05) than the other combination breeds. Feed intake in the Sabu x KUB group was the lowest, and their weight gain was the highest among the others group(P < 0.05). The combination Sabu x KUB also had the highest egg production, and hatchability. However, the percentage of abdominal fat of Sabu x KUB were higher than others groups. In conclusion, combination Sabu x KUB had the best growth performance and egg production performance, but not for the percentage of abdominal fat.


Genetika ◽  
2014 ◽  
Vol 46 (1) ◽  
pp. 95-104 ◽  
Author(s):  
Mladen Popovac ◽  
Milica Petrovic ◽  
Dragan Radojkovic ◽  
Dragan Stanojevic ◽  
Aleksandar Miletic ◽  
...  

The objective of this research paper was to make an assessment of breeding value of performance tested gilts of Swedish Landrace and F1 crossbreds of Swedish Landrace and Great Yorkshire by the method of selection index. The traits on whose basis the breeding value was estimated were: daily liveweight gain, average backfat thickness measured at two sites and carcass meat percentage. These traits were corrected for body mass of 100kg by the method of base indexes and the following average values were determined: corrected daily liveweight gain (KZDP) 408.93g/day, corrected average backfat thickness measured at two sites (KSL) 9.77mm and corrected carcass meat percentage (KPM) 61.08%. Studying the effect of genotype, year and birth season of gilts a statistically significant variation (P>0.05) of these traits provoked by the mentioned factors was not determined while the gilts` sire statistically highly significantly (P<0.001) influenced all studied traits. Heritability coefficients were: h2= 0.255 for KZDP, h2= 0.356 for KSL and h2 = 0.349 for KPM. The four selection index equations were constructed among which as the most optimal was chosen the one which includes all three traits (KZDP, KSL and KPM) and whose coefficient of the correlation of selection index and aggregate genotype was rIAG = 0.594.


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