scholarly journals A Study on Antimicrobial Activity of Sericin on Cotton Fabric

Author(s):  
Arunkumar K.V. ◽  
Gomathi K. ◽  
Deepika Priya A.

In the manufacturing of silk in the dried cocoons of a silkworm, fibroin is separated from sericin by a degumming process. The sericin is mostly discarded in the wastewater. The recovery of sericin not only reduces the environmental impact of silk manufacturing but a significant economic and social benefit could be realized from the recovery and recycling of sericin protein. Sericin has been studied as one of the new resources for textile and non-textile materials. It has properties like antioxidation, antibacterial, UV resistant, absorb and release of moisture, etc. Sericin has a good effect on wound healing, no toxicity, and low inflammatory reaction. Hence the present investigation aims to improve the antimicrobial activity of cotton fabric through the process of sericin finish.

2018 ◽  
Vol 51 (2) ◽  
pp. 647-663 ◽  
Author(s):  
Bobin Mi ◽  
Jing Liu ◽  
Yi Liu ◽  
Liangcong Hu ◽  
Yukun Liu ◽  
...  

Background/Aims: Antimicrobial peptides are effective promoters of wound healing but are susceptible to degradation. In this study, we replaced the GIGDP unit on the N-terminal of the endogenous human antimicrobial peptide hBD-2 with APKAM to produce A-hBD-2 and analyzed the effect on wound healing both in vitro and in vivo. Methods: The effects of A-hBD-2 and hBD-2 on cytotoxicity and proliferation in keratinocytes were assessed by Cell Counting Kit-8 assay. The structural stability and antimicrobial activity of hBD-2 and A-hBD-2 were evaluated against Staphylococcus aureus. RNA and proteins levels were evaluated by real-time PCR and western blotting, respectively. Cell migration was evaluated using a transwell assay. Cell cycle analysis was performed by flow cytometry. Wound healing was assessed in Sprague-Dawley rats. Epidermal thickness was evaluated by hematoxylin and eosin staining. Results: We found that hBD-2 exhibited cytotoxicity at high concentrations and decreased the structural stability in the presence of high sodium chloride concentrations. A-hBD-2 exhibited increased structural stability and antimicrobial activity, and had lower cytotoxicity in keratinocytes. A-hBD-2 increased the migration and proliferation of keratinocytes via phosphorylation of EGFR and STAT3 and suppressed terminal differentiation of keratinocytes. We also found that A-hBD-2 elicited mobilization of intracellular Ca2+ and stimulated keratinocytes to produce pro- and anti-inflammatory cytokines and chemokines via phospholipase C activation. Furthermore, A-hBD-2 promoted wound healing in vivo. Conclusion: Our data suggest that A-hBD-2 may be a promising candidate therapy for wound healing.


Water ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 821 ◽  
Author(s):  
Meimei Wu ◽  
Wei Ge ◽  
Zongkun Li ◽  
Zening Wu ◽  
Hexiang Zhang ◽  
...  

Despite the rapid development of risk analysis in dam engineering, there is a relative absence of research on the environmental impact of dam break. As a systematic theory, set pair analysis has a good effect in dealing with uncertainties, although the result is relatively rough and easy to distort. A connection degree of five grades and a generalized set of potential are introduced to improve traditional set pair analysis. Combined with the index system, the evaluation model of the environmental impact of dam break is established, which is based on generalized set pair analysis. Taking Sheheji Reservoir dam as an example, a comparison of evaluation results of fuzzy mathematics theory and generalized set pair analysis is made, which verifies the scientificity and practicability of the method proposed in this paper. The results show that the evaluation grade of the environmental impact of dam break at Sheheji Reservoir is serious, and appropriate management measures should be taken to reduce the risk.


Author(s):  
Mohd Riyaz Beg ◽  
Shital Ghodinde ◽  
Vidhi Gupta

In this changing world, we all are surrounded by the surmountable risk of getting injured. Amongst various risk factors, major burns are the most distressing and catastrophic. Burn wounds are not easy to heal via natural healing process and ultimately ended up with scar formation. If the degree of burn is high then the loss of tissue and its function is very common. To fasten-up the natural burn wound healing; zinc, an essential trace element is found to be very much effective. But due to its’ particle size limitation, less contact with wounded cells and tissues, and high inherent toxicity restrict its use. Needlessly, zinc is an element with dual action i.e. both antimicrobial and wound healing it is a prime choice to apply its aptitude in burn wound healing. To overcome the documented limitations zinc has converted to nanoparticle form. Zinc oxide nanoparticles, in particular, have attained ample of interest due to their unique properties and potential antimicrobial activity along with wound healing activity which makes it promising for the healing of topical burn wounds. Plant mediated green synthesis of nano-metal oxide particles is gaining a lot of significance due to its simplicity, eco-friendliness and extensive antimicrobial activity and recommended as an appealing substitute to not only physical methods but also chemical methods avoiding the use of the high rate of toxic chemicals and extreme surroundings. This study includes ZnO NPs role in burn wound healing with Phyto-mediated synthesis methods to provide evidence of their potential applications. Additionally, it provides an overview of traditional methods used for the synthesis of ZnO nanoparticles and characterization techniques to obtain information concerning the size, shape and optical properties along with toxicity and safety concern of ZnO NPs and its biomedical applications.


2020 ◽  
Vol 8 (32) ◽  
pp. 7197-7212 ◽  
Author(s):  
Zhiyong Qian ◽  
Yating Bai ◽  
Jin Zhou ◽  
Linhao Li ◽  
Jing Na ◽  
...  

The CTS-SF/SA/Ag-Exo dressing possessed multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.


Author(s):  
Kakaraparthy Ravishankar ◽  
Garlanka Venkata Naga Kiranmayi ◽  
Yejella Rajendra Prasad ◽  
Lankadi Devi

2012 ◽  
Vol 20 ◽  
pp. 77-88 ◽  
Author(s):  
Stanislav Kurajica ◽  
T. Očko ◽  
Vilko Mandić ◽  
V. Cigula Kurajica ◽  
I. Lozić

The simple and industrially adjustable process of silver nanoparticles deposition on cotton fabric followed by coating with γ-methacryloxypropyl trimethoxysilane (MPS), as well as the properties of obtained nanocomposite has been investigated. Silver nanoparticles were prepared through the reduction of silver nitrate with sodium borohydrate and deposited on cellulose fabric. In order to stabilize obtained nanostructured material and improve its properties, the fabric was treated by immersion in cross linkable MPS. The morphology of the finished fabrics was characterized by Scanning Electron Microscopy (SEM). It has been determined that process does not impair nanocharacter of silver particles and yield uniform distribution throughout the fabric surface. The concentration of Ag in the fabric samples was determined by Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). The average deposited amount of nanoparticles was found to be 63 ppm and 48 ppm for samples without and those with MPS, respectively. The character of obtained polysiloxane coating was examined using Fourier Transform Infrared (FTIR) spectroscopy. The presence of SiOSi bonds on the cellulose surface pointed out to self-condensation between silanol groups. Differential Thermal and Thermo-Gravimetric Analysis (DTA/TGA) revealed that MPS deposited on fabric provided modification of thermal properties. The change of the surface properties after the modification was ascertained by contact angle measurements. The antimicrobial activity of the antibacterial finish on the cotton fabric, expressed as bacterial reduction efficiency, has been determined. Bacterial reduction of at least 88.5% against both, Staphylococcus aureus and Escherichia coli, has been obtained.


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