Mutation Effect of Chemical Mutagen Ethymthane sulfonate (EMS) on Some Local Yeasts

2020 ◽  
Author(s):  
Badia Abdul Razzak ◽  
Rehan Nashwan Abul-Rahman ◽  
Maha Azad Hamid
Keyword(s):  
Candida Tropicalis ◽  
Orange Juice ◽  
Sensitivity Test ◽  
Chemical Mutagen ◽  
Cryptococcus Laurentii ◽  
Biochemical Tests ◽  
Rhodotorula Rubra ◽  
Local Markets ◽  
Trichosporon Asahii

Thirty samples of orange juice were collected from local markets in Mosul / Iraq. Isolates were diagnosed after performing phenotypic, culture and biochemical tests. The results showed that the yeasts belong to the following species: Rhodotorula rubra 36%, Trichosporon asahii 16%, Cryptococcus laurentii 28%, and Candida tropicalis 20%. The susceptibility of isolates to six antibiotics Candizole (Cd), Clotrimazole (Ct), Fluconazole (Fc), Ketoconazole (Kc), Lamisil (Ls), and Nystatin (Nys) was studied. The results of the sensitivity test showed that R. rubra was resistant to all antibiotics used except for Lamisil (Ls). The rest of the yeasts varied among themselves in their resist antibiotics. The chemical mutagen Ethyl Methanesulfate (EMS) at a concentration of 0.2 mg / ml on the vitality of the yeasts showed that the highest effect in the yeast Crypto. laurentii, with the killing severity reaching 4.47% while the lowest effected yeast was Tricho. asahii, with killing severity reaching 72%.

The comparative study among the MRSAcin, Nisin A and vancomycin, on biofilm formation by Methicillin resistance Staphylococcus aureus isolated from food sources.

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
¹Hind H. Muunim ◽  
Muna T Al-Mossawei ◽  
Mais Emad Ahmed
Keyword(s):  
Biofilm Formation ◽  
Methicillin Resistance ◽  
Microbial Control ◽  
Antibiotic Sensitivity ◽  
Raw Milk ◽  
Sensitivity Test ◽  
Food Sources ◽  
Resistant Bacteria ◽  
Biochemical Tests ◽  
Food Preservatives

Biofilms formation by pathogens microbial Control considered important in medical research because it is the hazarded virulence factor leading to becoming difficult to treat because of its high resistance to antimicrobials. Glycopeptide antibiotic a (Vancomycin) and the commercial bacteriocin (Nisin A) were used to comparative with purification bacteriocin (MRSAcin) against MRSA biofilm. One hundred food samples were collected from Baghdad markets from July 2016 to September 2016, including (cheese, yogurt, raw milk, fried meat, grilled meat, and beef burger). All samples were cultures; S. aureus was confirmation by macroscopic culture and microscopic examination, in addition to biochemical tests. Methicillin resistance S. asureus (MRSA) were identification by antibiotic sensitivity test (AST), Vitek 2 system. The result shown the 60(60%) isolate were identified as S. aureus and 45(75%) gave positive result as MRSA isolate, M13 isolate was chosen as MRSA isolates highest biofilm formation for treatment with MRSAcin, Nisin A(bacteriocin) and Vancomycin (antibiotic) to compared the more antimicrobial have bacteriocidal effect. The sensitivity test uses to determine the effect of MRSAcin, Nisin A, and Vancomycin MIC on MRSA planktonic cell by (WDA). The new study shows the impacts of new kind Pure Bacteriocins (MRSAcin) from methicillin-resistant S. aureus (MRSA) highly effects then (Vancomycin and Nisin A) at different concentration. In a current study aimed to suggest new Bacteriocin is potent highly for the treatment of resistant bacteria biofilm infections in food preservatives

scholarly journals Diversidade da microbiota de fungos da própolis in natura / Diversity of in natura propolis fungal microbiota

2021 ◽  
Vol 4 (4) ◽  
pp. 6369-6385
Author(s):  
Mariza Alves Ferreira ◽  
Rodrigo Arthur Fonseca Costa ◽  
Aline Simões da Rocha Bispo ◽  
Altino Branco Choupina ◽  
Norma Suely Evangelista-Barreto ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Candida Tropicalis ◽  
Cladosporium Cladosporioides ◽  
Penicillium Citrinum ◽  
Cryptococcus Laurentii ◽  
Aspergillus Versicolor ◽  
Paecilomyces Variotii ◽  
Candida Famata ◽  
Aspergillus Nomius ◽  
Fungal Microbiota

O presente estudo teve por objetivo realizar a identificação dos fungos encontrados na própolis produzido por Apis mellifera L. da Baía do Iguape, Brasil. Para tanto, foram utilizadas técnicas morfológicas, bioquímicas e moleculares, sendo averiguado o perfil de restrição gerado por espaçador interno transcrito (ITS1 e ITS4). O tamanho dos produtos de PCR foi analisado quanto ao perfil de restrição obtidos com endonuclease (HhaI, HaeIII e HinfI) por espécie. Foram identificadas dezesseis  espécies de fungos filamentosos: Flavodon flavus,  Aspergillus nomius, Aspergillus versicolor, Cladosporium sp., Coniothyrium sidae, Didymella sp., Paecilomyces variotii, Cladosporium cladosporioides, Penicillium citrinum, Fusarium incarnatum, Penicillium chermesinum, Phoma sp., Stagonosporopsis valerianellae, Phoma medicaginis, Paraphoma fimeti e Stagonosporopsis cucurbitacearum; e seis  espécies de leveduras: Candida tropicalis, Candida guiliermondii, Candida famata, Kodomala ohmeri, Trichosporon asahiu e Cryptococcus laurentii. Stagonosporopsis cucurbitacearum e leveduras pertencentes ao gênero Candida foram os microrganismos de maior ocorrência nas amostras da própolis provenientes da Baía do Iguape, Brasil.

DETERIORAÇÃO DE REFRIGERANTES POR LEVEDURAS

2004 ◽  
Vol 5 (2) ◽  
Author(s):  
C. D. Rocha ◽  
H. J. Schmidt ◽  
C. Monteiro ◽  
E. Odebrecht
Keyword(s):  
Soft Drink ◽  
Debaryomyces Hansenii ◽  
Rhodotorula Glutinis ◽  
Cryptococcus Laurentii ◽  
Rhodotorula Mucilaginosa ◽  
Candida Lipolytica ◽  
Rhodotorula Rubra ◽  
Zygosaccharomyces Bailii ◽  
Candida Sp ◽  
Saccharomycodes Ludwigii

Refrigerantes são bebidas não alcoólicas carbonatadas e constituem ótima fonte de glicídios. A composição química adocicada, o pH menor que 4,3, a aw maior que 0,90 e a atmosfera dos refrigerantes oferece condições favoráveis ao desenvolvimento de diversos microrganimos, incluindo leveduras deteriorantes..A deterioração ocasionada nos refrigerantes não constitui um risco à saúde das pessoas, mas este fenômeno prejudica a imagem de fábricas de refrigerantes, como também pode causar sérias perdas econômicas..Essas perdas podem ser minimizadas com o rastreamento da origem dos focos de contaminação, bem como conhecendo o risco potencial que cada levedura representa para o produto..As leveduras comumente detectadas em bebidas não alcoólicas carbonatadas, são as Brettanomyces sp, Candida lipolytica, Candida sp, Criptococcus albidus, Cryptococcus laurentii, Debaryomyces hansenii, Hanseniaspora sp, Hansenula sp, Kloeckera sp, Kluyveromyces sp, Zygosaccharomyces bailii, Zygosaccharomyces rouxii, Rhodotorula mucilaginosa, Rhodotorula glutinis, Rhodotorula rubra, Pichia sp, Saccharomyces cerevisae, Saccharomyces sp.,Saccharomycodes ludwigii, Schizosaccharomyces sp, e Zygosaccharomyces sp. SOFT DRINK DETERIORATION BY YEASTS Abstract Soft drinks are non alcoholic carbonated beverages that become an excellent source of glycids. Sugar composition, pH lower than 4.3, aw higher than 0.90 and atmosphere of soft drinks are conditions that contribute for the development of many microorganisms, including spoilage yeasts. Soft drink deterioration is not a health risk for people, but this phenomenon damages the beverage companies image and can yield serious economic damages. This problem could be reduced knowing the contamination focus origin, and also understanding the potential risk that each yeast represents to the product. The common yeasts found in nonalcoholic carbonated beverages are Brettanomyces sp, Candida lipolytica, Candida sp, Criptococcus albidus, Cryptococcus laurentii, Debaryomyces hansenii, Hanseniaspora sp, Hansenula sp, Kloeckera sp, Kluyveromyces sp, Zygosaccharomyces bailii, Zygosaccharomyces rouxii, Rhodotorula mucilaginosa, Rhodotorula glutinis, Rhodotorula rubra, Pichia sp, Saccharomyces cerevisae Saccharomyces sp, Saccharomycodes ludwigii, Schizosaccharomyces sp and Zygosaccharomyces sp.

Alicyclobacillus in Orange Juice: Occurrence and Heat Resistance of Spores

1999 ◽  
Vol 62 (8) ◽  
pp. 883-886 ◽  
Author(s):  
MIRTHA NELLY UBOLDI EIROA ◽  
VALÉRIA CHRISTINA AMSTALDEN JUNQUEIRA ◽  
FLÁVIO L. SCHMIDT
Keyword(s):  
Heat Resistance ◽  
Orange Juice ◽  
High Heat ◽  
Heat Treatments ◽  
Most Probable Number ◽  
Biochemical Tests ◽  
Probable Number ◽  
Death Time ◽  
Spore Activation ◽  
D Values

Spore suspensions of a pure culture of Alicyclobacillus acidoterrestris DSM 2498 were submitted to different heat treatments (60°C for 60 min, 60°C for 30 min, 70°C for 20 min, 80°C for 5 min, 80°C for 10 min, 80°C for 30 min, and boiling for 5 min) to determine the best activation conditions in orange juice. The best treatment for spore activation was shown to be 70°C/20 min. Seventy-five samples of concentrated orange juice from 11 different suppliers were examined for the presence of thermophilic acid-tolerant spore formers by the most probable number technique using Bacillus acidocaldarius medium (BAM broth) and incubation at 44°C for 5 days after a prior spore activation. After incubation, isolation was carried out using BAM agar medium incubating at 44°C for 5 days. Typical colonies were submitted to a microscopic examination, evaluation for the presence of spores, and various biochemical tests. Of the orange juice samples examined, 14.7% were found to be positive for Alicyclobacillus. The thermal death time open tube method was used to determine the heat resistance of the spores of strains confirmed as being Alicyclobacillus. The D-values determined were in the range from 60.8 to 94.5 min at 85°C, 10.0 to 20.6 min at 90°C, and 2.5 to 8.7 min at 95°C. The z-values were between 7.2°C and 11.3°C. The results demonstrated the occurrence of Alicyclobacillus in orange juice and the high heat resistance of the spores that could survive the heat treatments normally applied in the processing of orange juice.

scholarly journals Microbiological Quality of Prawns Collected from Local Markets of Dhaka Metropolis

2019 ◽  
Vol 35 (1) ◽  
pp. 13-16
Author(s):  
Anindita Bhowmik ◽  
Sharmistha Goswami ◽  
Sunjukta Ahsan
Keyword(s):  
Fecal Contamination ◽  
Microbiological Quality ◽  
Most Probable Number ◽  
Biochemical Tests ◽  
Probable Number ◽  
Local Markets ◽  
E Coli ◽  
Dilution Series ◽  
Agar Media

In Bangladesh, food sold at local markets are usually not kept under hygienic conditions leading to contamination with different microorganisms and deterioration of food quality. This study was carried out to examine the microbial quality of prawns sold at local markets. Prawn samples collected from different markets were assessed for their bacteriological quality using the multiple tube fermentation technique to determine coliform count using the most probable number method in Brilliant Green Lactose Broth (BGLB) media. Positive tubes of the presumptive test were further cultured on Eosine Methylene Blue(EMB) and Mac Conkey agar media. The organisms isolated were further characterized using biochemical tests. Out of the 65 samples, 47 (72.3%) showed positive results in all 3 tubes of dilution series using inoculum quantities of 1, 0.1 and 0.01 g.Among 65 samples 57 samples that contained at least one positive in each dilution series were further re-identified with biochemical tests.This study showed 56.14%isolates were Escherichia coli which conformed to expected biochemical reactions, formed round, small,elevated colonies with pink pigmentation on Mac Conkey agar media and round, small colonies with metallic green sheen pigmentation on EMB agar media. Other bacteria which presumptively appeared to be enterics and were isolated from BGLB were identified as Klebsiellapneumoniae(29.82%),Staphylococcus aureus(8.7%), Enterobacter aeroginosa(3.5%) and Salmonellatyphimurium (1.75%).Presumptive identification of E. coli in prawn in order to determine fecal contamination was able to identify ¾ of BGLB tubes with actual occurrence of E. coli. From this study it has been found that 97.14% bacteria were sensitive to Co-Trimoxazole, compared to other antibiotics used in this test whereas only 37.14% bacteria were sensitive to nitrofurantoin. This study also highlighted the fact that prawns act as a major source of E. coli which indicates possible fecal contamination as well as presence of potentially pathogenic E. coli and these bacteria are resistant at a great percentage to almost all of these antibiotics used in this study. Prawns must therefore be cooked adequately before consumption. Bangladesh J Microbiol, Volume 35 Number 1 June 2018, pp 13-16

scholarly journals ISOLATION AND IDENTIFICATION OF MICROORGANISMS FROM SOME SPOILED FRUITS AND VEGETABLES COLLECTED FROM NAGAON, ASSAM

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Dipanjali Devi ◽  
Rupjyoti Gogoi
Keyword(s):  
Fruits And Vegetables ◽  
Sufficient Condition ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Great Loss ◽  
Local Markets

Fruits and vegetables provide sufficient condition for the growth of several fungi and bacteria Microorganisms spoil the fruits and vegetables and changes the texture and makes them uneatable. There is a heavy loss of fruits and vegetables due to spoilage by microorganisms; however during handling and transportation also, there is a greater risk to get damaged .Thus farmers suffered a great loss due to spoilage. In the present study a total of about 3 spoiled fruits and 3spoiled vegetables were taken from some local markets of Nagaon, from which fungal and bacterial isolates were isolated and identified. Biochemical tests for the isolated bacteria were also done.

scholarly journals Summary Isolation and diagnosis of some fungal types from external ear canal infection cases with sensitivity test to some antifungal agents

2009 ◽  
Vol 33 (1) ◽  
pp. 82-88
Author(s):  
Dalya Abulkareem Abdulshaheed
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Candida Tropicalis ◽  
Antifungal Agents ◽  
Summer Season ◽  
Sensitivity Test ◽  
External Ear ◽  
Ear Canal ◽  
Sensitivity Tests

This research included isolation of some types of fungi of molds and yeasts from cases of inflammation ofexternal ear canal in human from AL- yarmouk Teaching Hospital, by collection of 50 samples from males andfemales and from different ages during summer season , also 10 samples from non infected cases were taken ascontrol. Also sensitivity test of some isolates against some important antifungal agents which commonly usedin the treatments of infection of external ear canale cases.After cultivation of isolates on saboroud dextrose agar, diagnosed as follows :9 isolates of Aspergillus niger, 6 isolates of Aspergillus fumigatus, 3 isolates of Aspergillus flavus, one isolate ofAspergillus terrus, 10 isolates of Candida albicans, 5 isolates of Candida tropicalis, 2 isolates of Candidaparapsilosis.The results of the sensitivity tests of some isolates against the antifungal agents appeared as follows: the isolatesof Aspergillus spp. were very sensitive to Clotrimazole and Econazole, while were less sensitive againstNystatine.Whereas the species of Candida were very sensitive to Nystatine and Econazole, but less sensitive againstClotrimazole.

scholarly journals BIOPROCESS APPLIED TO THE TREATMENT OF RESIDUAL WATERS OF COFFEE WET PROCESSING FOR THE INCORPORATION OF SELECTED YEAST STRAINS

2003 ◽  
Vol 21 (2) ◽  
Author(s):  
MARCIA REGINA BEUX ◽  
CARLOS ITSUO YAMAMOTO ◽  
CRISTINA MARA GUOLO WINTER ◽  
WILTON FONTES RAMOS ◽  
FABIANA NICOL BARBIERI ◽  
...  
Keyword(s):  
Candida Tropicalis ◽  
Residual Water ◽  
Kloeckera Apiculata ◽  
Organic Contamination ◽  
Rhodotorula Rubra ◽  
Dry Process ◽  
Yeast Strains ◽  
Wet Processing ◽  
Isolated Species

O objetivo deste trabalho foi isolar cepas de leveduras capazes de degradar a água de degomagem do beneficiamento úmido do café, caracterizando os metabólitos formados. A bebida preparada a partir de grãos tratados por via úmida é considerada de melhor qualidade quando comparada à elaborada com grãos tratados por via seca. No entanto, o alto potencial poluente da água residual do beneficiamento representa sério problema para o ambiente. A água de degomagem apresenta essencialmente contaminação orgânica, com taxas de demanda bioquímica de oxigênio (DBO) e demanda química de oxigênio (DQO) superiores a 20.000 mgO2/L. Candida tropicalis, C. lypolitica, C. guilliermondii, C. parapsilopsis, Kloeckera apiculata e Rhodotorula rubra, isoladas de cerejas de café, foram inoculadas em frascos contendo água de degomagem e esses, submetidos à agitação constante(120 rpm) por 48 horas, a 28oC. Candida parapsilopsis apresentou percentual de redução da taxa de DBO de 50,14% e de DQO de 29,81%, evidenciando a viabilidade do uso desses microrganismos como biodespoluentes. DESENVOLVIMENTO DE BIOPROCESSO APLICADO AO TRATAMENTO DE ÁGUAS RESIDUAIS DO BENEFICIAMENTO ÚMIDO DO CAFÉ PELA INCORPORAÇÃO DE LINHAGEM SELECIONADA DE LEVEDURAS Abstract The aim of the present work was to isolate yeast strains able to degrade the degumming water originated from the wet processing of coffee, characterizing the formed metabolites. The drink prepared from the treated grains by wet processing is considered of better quality when compared with the grains treated by dry process. However, the great polluent potential of the residual water from this processing represents serious concern for the environment. The degumming water essentially presents organic contamination, with Oxygen Biochemical Demand (OBD) and Oxygen Chemical Demand (OCD) rates superior to 20.000 mgO2/L. Candida tropicalis, C. lypolitica, C. guilliermondii, C. parapsilopsis, Kloeckera apiculata e Rhodotorula rubra, isolated from coffee cheeries were inoculated in flasks containing the degumming water and then submitted to constant agitation (120 rpm) for 48 hours at 28ºC. The isolated species revealed capacity to degrade the substrate, however C.parapsilopsis showed reduction percentages of OBD and OCD rates of 50.14% e 29.81%, respectivelly, showing the viability of the use of those microorganisms as biological depollutants.

scholarly journals Detection of Salmonella typhimurium in chicken meat imported in the local markets of Diwaniya city By using PCR technique

2013 ◽  
Vol 12 (2) ◽  
pp. 133
Author(s):  
A.A. Saeed
Keyword(s):  
Chicken Meat ◽  
Chain Reaction ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Local Markets ◽  
Turkish Origin ◽  
Chromogenic Agar ◽  
Biochemical Identification ◽  
Polymerase Chain ◽  
Meat Samples

The aim of this study to detected contamination with Salmonella spp. In imported chicken meat in the local markets of Al- Diwaniyia city . to protect health of consumer and Determintion the most contaminated origin with salmonella spp. A toatl of 100 chicken meat samples collected from different origin. The bacteria cultured and isolated in enrichment and selective media . Salmonella isolates were subjected to some biochemical tests show positive productive results H2S .TSI . SIM And its give negative for indole , vo-gs Proskauer and ureas , Biochemical identification was carried out using API 20-E test ..the result showed isolation sample (33\55)60% on bismuth sulphate agar and the results of isolation on chromogenic agar were 87.8 |%(29\33) .according to reading Api20-E system the results of confirmation of isolates 92%(25\26) In this study,( 23) Salmonella isolates were detected by polymerase chain reaction (PCR) by using 16s rRNA and invA gene these primers were selected specifically for the detection of Salmonella to amplify a 406bp and 558 bp DNA fragments, respectively. Only 7 isolates out of 23 were identified as S. typhimurium the results of this study showed the highest percent of s.typhimurim isolates was ( 50%) ( 3/6) for India origin and the lowest was Turkish origin

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