scholarly journals Diversidade da microbiota de fungos da própolis in natura / Diversity of in natura propolis fungal microbiota

2021 ◽  
Vol 4 (4) ◽  
pp. 6369-6385
Author(s):  
Mariza Alves Ferreira ◽  
Rodrigo Arthur Fonseca Costa ◽  
Aline Simões da Rocha Bispo ◽  
Altino Branco Choupina ◽  
Norma Suely Evangelista-Barreto ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Candida Tropicalis ◽  
Cladosporium Cladosporioides ◽  
Penicillium Citrinum ◽  
Cryptococcus Laurentii ◽  
Aspergillus Versicolor ◽  
Paecilomyces Variotii ◽  
Candida Famata ◽  
Aspergillus Nomius ◽  
Fungal Microbiota

O presente estudo teve por objetivo realizar a identificação dos fungos encontrados na própolis produzido por Apis mellifera L. da Baía do Iguape, Brasil. Para tanto, foram utilizadas técnicas morfológicas, bioquímicas e moleculares, sendo averiguado o perfil de restrição gerado por espaçador interno transcrito (ITS1 e ITS4). O tamanho dos produtos de PCR foi analisado quanto ao perfil de restrição obtidos com endonuclease (HhaI, HaeIII e HinfI) por espécie. Foram identificadas dezesseis  espécies de fungos filamentosos: Flavodon flavus,  Aspergillus nomius, Aspergillus versicolor, Cladosporium sp., Coniothyrium sidae, Didymella sp., Paecilomyces variotii, Cladosporium cladosporioides, Penicillium citrinum, Fusarium incarnatum, Penicillium chermesinum, Phoma sp., Stagonosporopsis valerianellae, Phoma medicaginis, Paraphoma fimeti e Stagonosporopsis cucurbitacearum; e seis  espécies de leveduras: Candida tropicalis, Candida guiliermondii, Candida famata, Kodomala ohmeri, Trichosporon asahiu e Cryptococcus laurentii. Stagonosporopsis cucurbitacearum e leveduras pertencentes ao gênero Candida foram os microrganismos de maior ocorrência nas amostras da própolis provenientes da Baía do Iguape, Brasil.

LEVEDURAS EM CASOS DE MASTITE BOVINA SUBCLÍNICA NO SUL DO RIO GRANDE DO SUL

2021 ◽  
Vol 11 (2) ◽  
pp. 803-807
Author(s):  
Pedro Rassier dos Santos ◽  
Carolina Lambrecht Gonçalves ◽  
Giniani Carla Dors ◽  
Helenice De Lima Gonzalez ◽  
Patrícia Da Silva Nascente
Keyword(s):  
Candida Parapsilosis ◽  
Rio Grande ◽  
Cryptococcus Laurentii ◽  
Rio Grande Do Sul ◽  
California Mastitis Test ◽  
Candida Famata ◽  
Vitek 2

Alguns alimentos como o leite tem um importante papel na dieta humana, pois fornece uma série de nutrientes essenciais. A contaminação do leite por micro-organismos indesejáveis, como os fungos, pode causar alterações físico-químicas no mesmo, limitando sua durabilidade e de seus derivados, além de determinar problemas econômicos e de saúde pública. As leveduras são os agentes fúngicos mais frequentemente relacionados às infecções da glândula mamária em bovinos de leite. Para realização deste trabalho, foram coletadas 640 amostras de leite, oriundas dos quartos mamários de vacas em lactação que apresentaram resultado positivos ao Califórnia Mastitis Test (CMT). Previamente foi realizada a desinfecção do teto com algodão embebido em álcool 70ºGL e posteriormente retirou-se as amostras de aproximadamente 10 mL de leite que foram encaminhadas para o Laboratório de Micologia, no Instituto de Biologia da UFPel, semeadas em placas de Petri contendo Ágar Sabouraud Dextrose adicionado de Cloranfenicol e armazenadas na estufa por 7 dias a 36 °C. Nas amostras em que houve crescimento leveduriforme, fez-se a identificação através do Sistema Vitek 2. Foram identificados: Cryptococcus laurentii, Candida famata e Candida parapsilosis.

scholarly journals Optimizing the tray dryer temperature and time of white corn flour culture

Food Research ◽  
2021 ◽  
Vol 5 (5) ◽  
pp. 95-104
Author(s):  
D. Hunaefi ◽  
Rahmawati R. ◽  
D. Saputra ◽  
R.R. Maulani ◽  
T. Muhandri
Keyword(s):  
Response Surface Methodology ◽  
Aspergillus Niger ◽  
Water Content ◽  
Response Surface ◽  
Water Activity ◽  
Penicillium Citrinum ◽  
Drying Process ◽  
Corn Flour ◽  
Acremonium Strictum ◽  
Candida Famata

This research aimed to optimize the tray dryer temperature and time of white corn flour culture by Response Surface Methodology (RSM). There were two cultures used in this research, namely Amylolytic Culture (AC) and Complete Culture (CC). AC consisted of Penicillium citrinum, Aspergillus niger, Acremonium strictum, and Candida famata, while CC consisted of Penicillium chrysogenum, Penicillium citrinum, Aspergillus niger, Rhizopus stolonifer, Rhizopus oryzae, Fusarium oxysporum, Acremonium strictum, Candida famata, Kodamaea ohmeri and Candida krusei/incospicua. The independent variables in this study were drying temperature and time, where the quality indicators used were total viability of mold and yeast, water content, water activity, and pH. This research used a factor response surface methodology. Data were analyzed by ANOVA with an α level of 95%. The result of this research showed that the optimum drying process for AC starter was 40°C for 10 hrs, with characteristic response viability 8.8×107 CFU/g, water activity 0.43, water content 8.90%, and pH 4.05. CC starter showed an optimum drying process at 49°C for 4.5 hrs, with characteristic response viability 4.9×107 CFU/g, water activity 0.49, water content 7.02%, and pH 3.95. The optimum tray dryer temperatures and times were achieved for AC and CC starters.

Isolation and identification of microbiota from Egyptian common insect pests invading economically important crops

2021 ◽  
pp. 5825-5830
Author(s):  
Waill A. Elkhateeb ◽  
Marwa O. ELnahas ◽  
Kareem M. Mousa ◽  
Adel A. Ahmed ◽  
Abdu Galib ALKolaibe ◽  
...  
Keyword(s):  
Insect Pests ◽  
Agrotis Ipsilon ◽  
Cotton Leaf ◽  
Paecilomyces Variotii ◽  
Isolation And Identification ◽  
Black Cutworm ◽  
Absidia Corymbifera ◽  
Control Programs ◽  
Fungal Microbiota ◽  
Bacteria And Fungi

Insects bodies contain multiple distinctive microbial colonies which play key role in the metabolism, development and health of an insect. In the current study bacterial and fungal microbiota were isolated from larval feces and adult wings of two major insect pests in Egypt, the Cotton leaf worm Spodoptera littoralis and the black cutworm Agrotis ipsilon. Isolated pathogens were identified and characterized according to the scientific described methods in such cases. Two different genera of bacteria (Bacillus & Serratia) were isolated from larval feces of both insects while isolated fungus were Paecilomyces variotii and Absidia corymbifera from the feces of A. ipsilon larvae, while Rhozopus stolonifer and Penicillium chrysogenum were isolated from adults wings of the same insect. The fungus Aspergillus flavus, Aspergillus niger and Mucor circinilloides were isolated from feces of S. littoralis larvae. Numerous species of bacteria and fungi had been documented as a bio-agent against many insect pests, and most of them exhibited disruption in life-cycle of different species of Lepidopterous pests. The obtained data sheds light on microbial colonies associated with two major insect pests, however further studies are required to involve the isolated microbiota in the biological control programs of insect pests.

scholarly journals Yeast microbiota of free-ranging amphibians and reptiles from Caatinga biome in Ceará State, Northeast Brazil: High pathogenic potential of Candida famata

2021 ◽  
Vol 51 (7) ◽  
Author(s):  
Débora de Souza Collares Maia Castelo-Branco ◽  
Renan Vasconcelos da Graça-Filho ◽  
Jonathas Sales de Oliveira ◽  
Maria Gleiciane da Rocha ◽  
Géssica dos Santos Araújo ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Virulence Factors ◽  
Antifungal Susceptibility ◽  
Hydrolytic Enzymes ◽  
Protease Production ◽  
Pathogenic Potential ◽  
Candida Famata ◽  
Caatinga Biome ◽  
Fungal Microbiota ◽  
Free Ranging

ABSTRACT: Studies on the fungal microbiota of reptiles and amphibians are necessary to better understand of host-microbe interactions and the establishment of fungal disease in these animals. However, these studies are limited. The present researchidentified yeasts from free-ranging reptiles and amphibians from the Caatinga biome andevaluated the virulence factors production, the antifungal susceptibility in planktonic and biofilm growth and the pathogenicity of Candida famata isolates. Twenty-nine isolates of the genera Candida, Cryptococcus and Rhodotorula were identified by phenotypic and/or molecular methods and production of hydrolytic enzymes in vitro by these genera of fungi was evaluated. In addition, susceptibility of planktonic cells and biofilms to azoles and amphotericin B was evaluated. The pathogenicity of C. famata, the most prevalent yeast species isolated, was evaluated using Caenorhabditis elegans model. C. famata was the most prevalent yeast in amphibian and reptilian microbiota. Phospholipase and protease production was observed in 18/29 and 11/29 of the yeast isolates, respectively, while 100% formed biofilms. Itraconazole presented high minimal inhibitory concentrations against C. famata and C. tropicalis. Amphotericin B reduced the biomass and metabolic activity of biofilms. C. famata induced the mortality of C. elegans. In conclusion, reptiles and amphibians are colonized by yeasts capable of producing important virulence factors, especially by Candida spp. that present low susceptibility to azoles which may result from imbalances in ecosystem. Finally, C. famata isolated from these animals presented high pathogenicity, showing the importance of the study of reptile and amphibians fungal microbiota.

scholarly journals Culturable fungi associated with the marine shallow-water hydrothermal vent crab Xenograpsus testudinatus at Kueishan Island, Taiwan

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ami Shaumi ◽  
U-Cheng Cheang ◽  
Chieh-Yu Yang ◽  
Chic-Wei Chang ◽  
Sheng-Yu Guo ◽  
...  
Keyword(s):  
Shallow Water ◽  
Hydrothermal Vent ◽  
Potential Role ◽  
Candida Parapsilosis ◽  
Additional Data ◽  
Penicillium Citrinum ◽  
Aspergillus Versicolor ◽  
Marine Animals ◽  
Culturable Fungi ◽  
Infections Diseases

Abstract Reports on fungi occurring on marine crabs have been mostly related to those causing infections/diseases. To better understand the potential role(s) of fungi associated with marine crabs, this study investigated the culturable diversity of fungi on carapace of the marine shallow-water hydrothermal vent crab Xenograpsus testudinatus collected at Kueishan Island, Taiwan. By sequencing the internal transcribed spacer regions (ITS), 18S and 28S of the rDNA for identification, 12 species of fungi were isolated from 46 individuals of X. testudinatus: Aspergillus penicillioides, Aspergillus versicolor, Candida parapsilosis, Cladosporium cladosporioides, Mycosphaerella sp., Parengyodontium album, Penicillium citrinum, Penicillium paxili, Stachylidium bicolor, Zasmidium sp. (Ascomycota), Cystobasidium calyptogenae and Earliella scabrosa (Basidiomycota). With additional data from other published reports, a total of 26 species of fungi (23 Ascomycota, three Basidiomycota) have been recorded from X. testudinatus. Aspergillus is the most speciose genus on the crab, followed by Penicillium and Candida. All but one species (Xylaria arbuscula) had been previously isolated from substrates in the marine environment, although many are typical terrestrial taxa. None of the recorded fungi on X. testudinatus are reported pathogens of crabs, but some have caused diseases of other marine animals. Whether the crab X. testudinatus is a vehicle of marine fungal diseases requires further study.

Incidence of Toxic and Other Mold Species and Genera in Soybeans

1977 ◽  
Vol 40 (5) ◽  
pp. 309-312 ◽  
Author(s):  
PHILIP B. MISLIVEC ◽  
VERNEAL R. BRUCE
Keyword(s):  
Aspergillus Nidulans ◽  
Penicillium Chrysogenum ◽  
Penicillium Citrinum ◽  
Aspergillus Versicolor ◽  
Surface Disinfection ◽  
Aspergillus Glaucus ◽  
Penicillium Cyclopium ◽  
Before And After ◽  
Aspergillus Candidus

The mold flora of 385 unprocessed soybean samples was determined before and after surface disinfection of the beans with 5% NaOCl. Molds were detected in 99.4% of the 19,250 non-disinfected soybeans examined, and in 52.8% of the 19,250 surface-disinfected soybeans examined indicating that contamination was primarily at the surface, although internal invasion also was substantial. The mold flora, both before and after surface disinfection, was dominated by species of the Aspergillus glaucus group, Asperigillus flavus, Aspergillus candidus, Penicillium cyclopium, plus species of Alternaria and Cladosporium. The toxicogenic species A. flavus, Aspergillus nidulans, Aspergillus ochaeceus, Aspergillus versicolor, Penicillium citrinum, P. cyclopium, and Pencillium viridicatum were encountered regularly in non-surface-disinfected soybeans. Except for A. flavus and P. cyclopium, the occurrence of toxicogenic species Aspergillus melleus, soybeans was sporadic. The toxicogenic species Aspergillus melleus, Pencillium expansum, and Penicillium urticae were encountered occasionally, but only in non-surface-disinfected soybeans. Penicillium chrysogenum, the penicillin-producing organism, was encountered regularly in non-surface-disinfected soybeans, but less frequently in disinfected soybeans.

Mutation Effect of Chemical Mutagen Ethymthane sulfonate (EMS) on Some Local Yeasts

2020 ◽  
Author(s):  
Badia Abdul Razzak ◽  
Rehan Nashwan Abul-Rahman ◽  
Maha Azad Hamid
Keyword(s):  
Candida Tropicalis ◽  
Orange Juice ◽  
Sensitivity Test ◽  
Chemical Mutagen ◽  
Cryptococcus Laurentii ◽  
Biochemical Tests ◽  
Rhodotorula Rubra ◽  
Local Markets ◽  
Trichosporon Asahii

Thirty samples of orange juice were collected from local markets in Mosul / Iraq. Isolates were diagnosed after performing phenotypic, culture and biochemical tests. The results showed that the yeasts belong to the following species: Rhodotorula rubra 36%, Trichosporon asahii 16%, Cryptococcus laurentii 28%, and Candida tropicalis 20%. The susceptibility of isolates to six antibiotics Candizole (Cd), Clotrimazole (Ct), Fluconazole (Fc), Ketoconazole (Kc), Lamisil (Ls), and Nystatin (Nys) was studied. The results of the sensitivity test showed that R. rubra was resistant to all antibiotics used except for Lamisil (Ls). The rest of the yeasts varied among themselves in their resist antibiotics. The chemical mutagen Ethyl Methanesulfate (EMS) at a concentration of 0.2 mg / ml on the vitality of the yeasts showed that the highest effect in the yeast Crypto. laurentii, with the killing severity reaching 4.47% while the lowest effected yeast was Tricho. asahii, with killing severity reaching 72%.

scholarly journals Dectin-1 and IL-17A Suppress Murine Asthma Induced by Aspergillus versicolor but Not Cladosporium cladosporioides Due to Differences in β-Glucan Surface Exposure

2012 ◽  
Vol 189 (7) ◽  
pp. 3609-3617 ◽  
Author(s):  
Rachael A. Mintz-Cole ◽  
Aaron M. Gibson ◽  
Stacey A. Bass ◽  
Alison L. Budelsky ◽  
Tiina Reponen ◽  
...  
Keyword(s):  
Cladosporium Cladosporioides ◽  
Aspergillus Versicolor

Yeast microbiota of natural cavities of manatees (Trichechus inunguis and Trichechus manatus) in Brazil and its relevance for animal health and management in captivity

2015 ◽  
Vol 61 (10) ◽  
pp. 763-769 ◽  
Author(s):  
José Júlio Costa Sidrim ◽  
Vitor Luz Carvalho ◽  
Débora de Souza Collares Maia Castelo-Branco ◽  
Raimunda Sâmia Nogueira Brilhante ◽  
Tereza de Jesus Pinheiro Gomes Bandeira ◽  
...  
Keyword(s):  
Animal Health ◽  
Sensu Stricto ◽  
Candida Guilliermondii ◽  
Cryptococcus Laurentii ◽  
Trichechus Manatus ◽  
Sabouraud Agar ◽  
Candida Metapsilosis ◽  
Candida Famata ◽  
In Captivity ◽  
Candida Orthopsilosis

The aim of this study was to characterize the yeast microbiota of natural cavities of manatees kept in captivity in Brazil. Sterile swabs from the oral cavity, nostrils, genital opening, and rectum of 50 Trichechus inunguis and 26 Trichechus manatus were collected. The samples were plated on Sabouraud agar with chloramphenicol and incubated at 25 °C for 5 days. The yeasts isolated were phenotypically identified by biochemical and micromorphological tests. Overall, 141 strains were isolated, of which 112 were from T. inunguis (Candida albicans, Candida parapsilosis sensu stricto, Candida orthopsilosis, Candida metapsilosis, Candida guilliermondii, Candida pelliculosa, Candida tropicalis, Candida glabrata, Candida famata, Candida krusei, Candida norvegensis, Candida ciferri, Trichosporon sp., Rhodotorula sp., Cryptococcus laurentii) and 29 were from T. manatus (C. albicans, C. tropicalis, C. famata, C. guilliermondii, C. krusei, Rhodotorula sp., Rhodotorula mucilaginosa, Rhodotorula minuta, Trichosporon sp.). This was the first systematic study to investigate the importance of yeasts as components of the microbiota of sirenians, demonstrating the presence of potentially pathogenic species, which highlights the importance of maintaining adequate artificial conditions for the health of captive manatees.

scholarly journals Síntese e avaliação de azoderivado de 2-fenilbenzoxazol como substrato fluorogênico na diferenciação de Candida spp.

2019 ◽  
Vol 9 (1) ◽  
pp. 28-39
Author(s):  
Priscilla De Bastos Souza ◽  
Valeriano Antonio Corbellini
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Candida Glabrata ◽  
Candida Parapsilosis ◽  
Candida Guilliermondii ◽  
Candida Krusei ◽  
Candida Spp ◽  
Candida Famata ◽  
Ft Ir

Neste estudo foram sintetizados três derivados de 2-fenilbenzoxazol (2FBO) funcionalizados com a função azo: (2’-hidroxi-5’-fenilazofenil)benzoxazol (HFAZBO), 2-(2’-hidroxi-5’-(4’’-carboxi)fenilazofenil)benzoxazol (HFAZBO-CO2H) e 2-(2’-hidroxi-5’-(4’’-nitro)fenilazofenil)benzoxazol (HFAZBO-NO2).Os compostos foram utilizados como substratos fluorogênicos para agentes de infecção urinária, representantes de Candida spp., para explorar suas características metabólicas. A caracterização estrutural dos compostos foi realizada por meio de Espectroscopia de Absorção Molecular no Infravermelho com transformada de Fourier (FT-IR) e por determinação da faixa de fusão. Os ensaios fluorogênicos foram realizados em espécies de Candida spp.: Candida albicans ATCC 10231, Candida famata RL38, Candida glabrata 06130L, Candida glabrata RL43, Candida glabrata 0030L, Candida guilliermondii TH07, Candida krusei ATCC 6258, Candida krusei 0037L, Candida parapsilosis (RL33), Candida parapsilosis (RL38), Candida stellatoidea (9875), Candida stellatoidea (3613), Candida tropicalis ATCC 750. Não foi observada alteração sob luz uv nas leveduras testadas. Do mesmo modo, o extrato obtido a partir das colônias não apresentou fluorescência sob luz uv. Dessa forma, faz-se necessário desenvolver novos estudos que avaliem a função metabólica de Candida spp. a partir da biotransformação levando a liberação de moléculas fluorescentes.

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