scholarly journals SEROLOGICAL EXAMINATION OF PARVOVIRUS INFECTION ON PIG FARMS

2009 ◽  
Vol 2 (1) ◽  
pp. 45-53
Author(s):  
Diana Lupulović ◽  
Sava Lazić ◽  
Tamaš Petrović ◽  
Jasna Prodanov
Keyword(s):  
Antibody Titre ◽  
Haemagglutination Inhibition ◽  
Economic Losses ◽  
High Antibody ◽  
Reproductive Disorders ◽  
Serological Examination ◽  
Causative Agents ◽  
High Antibody Titre ◽  
Intensive Breeding ◽  
Titre Antibody

Porcine parvovirus (PPV) is one of the causative agents in reproductive disorders in swine that causes great economic losses. The objective of our research was to determine prevalence of this infection on farms with intensive breeding in 5 municipalities in Vojvodina. The research encompassed 19 herds with a total of 344 blood samples screened for the presence of antibodies against PPV (gilts, breeding sows and boars). Serological examination was carried out by haemagglutination inhibition test (HI test). After the analyses of the results, seropositive animals were detected on the farms and there were no PPV-free farms. The determined antibody titre against PPV ranged from 1:64 to 1:16384. Out of 344 examined animals, there were 305 (88.66%) with a high antibody titre (1:1024 to 1:16384). The highest level of antibodies (1:16384) was detected in 145 animals, what is 42.15% of total number. In 24 animals (6.97%) titre antibody was 1:512 and these animals were of low seropositivity. Simultaneously, a comparison of antibodies in blood sera was carried out on 20 gilts (Table 3, farm 2) originating from a farm where the animals were vaccinated against PPV and 20 unvaccinated gilts (Table 4, farm 1) originating from a farm without vaccination against PPV. In unvaccinated animals antibody titre was unequal and ranged from 1:256 to 1:16384, in unvaccinated animals the values were considerably higher and ranged from 1:4096 do 1:16384, and in 17 out of 20 vaccinated pigs (85%) the highest antibody titre was 1:16384.

scholarly journals Antibody Response and Haematological Changes of Nigerian Locally Adapted Turkeys to Sheep Red Blood Cells (SRBC)

2020 ◽  
Vol 77 (2) ◽  
pp. 20
Author(s):  
Ayodele Emmanuel OGUNDERO ◽  
Mofoyeke Oluwayemisi SANDA ◽  
Adeyemi Sunday ADENAIKE ◽  
Michael Irewole TAKEET ◽  
Christian Obiora Ndubuisi IKEOBI
Keyword(s):  
Red Blood Cells ◽  
Antibody Titre ◽  
Blood Cells ◽  
White Blood Cells ◽  
Primary Response ◽  
Haematological Parameters ◽  
High Antibody ◽  
Sheep Red Blood Cells ◽  
Antibody Titres ◽  
High Antibody Titre

Haemagglutination assay and haematological analysis of 143 poults generated as F1 individuals by artificial insemination from randomly selected turkeys of White, Black and Lavender genotypes which are classified by antibody titre was carried out so as to confirm their antibody titre levels in response to sheep red blood cells (SRBC). Results showed that mean values obtained for high and low antibody titres were 7.31 and 2.67 respectively, resulting in the classification of the turkeys into Black high and low, Lavender high and black, and White high and low antibody titres. The genotype’s titre had significant (P <0.05) effect on the packed cell volume (PCV), haemoglobin (Hb), red blood cells (RBC), white blood cells (WBC) and basophil (BAS) of the basal haematological parameters. Genotype’s titre had no significant (P >0.05) effect on the primary response haematological parameters. Meanwhile, the primary response haematological parameters to SRBC antigen varied along the genotypes with the WBC increasing drastically in all the genotypes, signifying the presence of an antigen. The study concluded that the F1 turkey poult population studied diverged along the high and low antibody titre in response to SRBC. Thus, the F1 generation of the high antibody titre genotypes (Black high, White high and Lavender high) can be used as foundation stock for selection of local turkeys for high antibody titre.

>CASE REPORT: Eosinophilic colitis with high antibody titre against Ascaris suum

1997 ◽  
Vol 12 (3) ◽  
pp. 204-206 ◽  
Author(s):  
YASUAKI TAKEYAMA ◽  
SEIICHIRO KAMIMURA ◽  
JUNJI SUZUMIYA ◽  
KOHJI OH ◽  
MAKOTO OKUMURA ◽  
...  
Keyword(s):  
Case Report ◽  
Antibody Titre ◽  
Ascaris Suum ◽  
High Antibody ◽  
Eosinophilic Colitis ◽  
High Antibody Titre

scholarly journals A persistent epidemic of porcine epidemic diarrhoea virus infection by serological survey of commercial pig farms in northern Vietnam

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Ohnmar Myint ◽  
Nguyen Thi Hoa ◽  
Naoyuki Fuke ◽  
Apisit Pornthummawat ◽  
Nguyen Thi Lan ◽  
...  
Keyword(s):  
Antibody Titre ◽  
Farm Size ◽  
High Antibody ◽  
Serological Survey ◽  
Serological Evidence ◽  
Northern Vietnam ◽  
Porcine Epidemic Diarrhoea Virus ◽  
Diarrhoea Virus ◽  
Pig Farms ◽  
High Antibody Titre

Abstract Background Porcine epidemic diarrhoea (PED) is a highly contagious infectious disease with negative economic impacts on the swine industry. PED outbreaks were reported from 2009 to 2015, but sporadic infection has been observed until now in Vietnam. However, the seroprevalence of PEDV infection has not yet been reported for commercial pig farms in Vietnam. The aim of this study was to assess the seroprevalence of PEDV infection in Vietnamese pig farms to reveal the endemic status of PEDV in northern Vietnam. Results A serological survey of PEDV infection was carried out using indirect ELISA in commercial pig farms in Hai Duong, Hung Yen and Thai Binh provinces in northern Vietnam in 2019. Twenty sera were randomly collected from each of 10 commercial pig farms, from each province; none of the farms had vaccinated for PEDV. Serological evidence of natural PEDV infection, expressed as a high antibody titre, was observed in the pig farms in all 3 provinces. The OD values were significantly higher (p < 0.001) for pig sera from Thai Binh than from Hai Duong and Hung Yen. No significant differences (p > 0.05) were detected for seropositivity to PEDV based on locality, age, pig breed and farm size. Conclusions This study indicates serological evidence of natural PEDV infection with high antibody titre in commercial pig farms. PEDV infection was widespread among the pig population in these 3 provinces and that good management and strict biosecurity are needed at these pig farms.

scholarly journals Diversity of Colletotrichum Species Associated with Anthracnose Disease in Tropical Fruit Crops—A Review

Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 297
Author(s):  
Latiffah Zakaria
Keyword(s):  
Management Strategies ◽  
Economic Losses ◽  
Fruit Crops ◽  
Tropical Fruit ◽  
Colletotrichum Species ◽  
Adequate Knowledge ◽  
Dragon Fruit ◽  
Causative Agents ◽  
Colletotrichum Spp ◽  
Fruit Plantations

In tropical fruit crops, anthracnose is mainly caused by species belonging to the fungal genus, Colletotrichum. These phytopathogens can infect several parts of the fruit crops; however, infection during postharvest or ripening stages is responsible for major economic losses. Due to the formation of black to dark brown sunken lesions on the fruit surface, anthracnose reduces fruit quality and marketability. Among the most common tropical fruit crops susceptible to anthracnose are mango, papaya, banana, avocado, guava, and dragon fruit; these are economically relevant products in many developing countries. It is important to document that the newly recorded Colletotrichum spp. associated with fruit anthracnose can infect multiple hosts, but some species may be host-specific. By using multiple markers, many phylogenetic species of Colletotrichum have been reported as anthracnose-causing pathogens. Taking into account that disease management strategies strongly rely on adequate knowledge of the causative agents, updated information on Colletotrichum species and the hazard posed by the most recently identified species in tropical fruit plantations and harvested fruits becomes vital. Besides, the newly recorded species may be important for biosecurity and should be listed as quarantine pathogens, considering that tropical fruits are traded worldwide.

scholarly journals Poult Enteritis and Mortality Syndrome in Turkey Poults: Causes, Diagnosis and Preventive Measures

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2063
Author(s):  
Awad A. Shehata ◽  
Shereen Basiouni ◽  
Reinhard Sting ◽  
Valerij Akimkin ◽  
Marc Hoferer ◽  
...  
Keyword(s):  
Preventive Measures ◽  
Clinical Signs ◽  
Accurate Diagnosis ◽  
Economic Losses ◽  
Turkey Poults ◽  
Causative Agents ◽  
Enteric Disorders ◽  
And Control ◽  
Enteropathogenic Viruses ◽  
Poult Enteritis

Poult enteritis and mortality syndrome (PEMS) is one of the most significant problem affecting turkeys and continues to cause severe economic losses worldwide. Although the specific causes of PEMS remains unknown, this syndrome might involve an interaction between several causative agents such as enteropathogenic viruses (coronaviruses, rotavirus, astroviruses and adenoviruses) and bacteria and protozoa. Non-infectious causes such as feed and management are also interconnected factors. However, it is difficult to determine the specific cause of enteric disorders under field conditions. Additionally, similarities of clinical signs and lesions hamper the accurate diagnosis. The purpose of the present review is to discuss in detail the main viral possible causative agents of PEMS and challenges in diagnosis and control.

scholarly journals Postnatal decline of maternally acquired viral antibodies of different specificities

1971 ◽  
Vol 69 (3) ◽  
pp. 435-444 ◽  
Author(s):  
M. J. Cloonan ◽  
R. A. Hawkes ◽  
L. H. Stevens
Keyword(s):  
Antibody Titre ◽  
Half Life ◽  
Influenza A ◽  
High Antibody ◽  
Serum Antibodies ◽  
Rectangular Hyperbola ◽  
Viral Antibodies ◽  
Antibody Titres ◽  
Type 3 ◽  
Rubella Antibody

SUMMARYThe rates of decline (half-lives) of maternally acquired antibodies of two different specificities in a group of infants were found to be highly variable, ranging from 18 to 192 days for parainfluenza type 3 antibody (54 infants) and from 15 to 251 days for influenza A2 antibody (nine infants). For antibodies of both specificities approximately 75% of the half-lives were between 15 and 60 days. With parainfluenza type 3 antibody, and possibly with influenza A 2 antibody, the half-lives were inversely proportional to the initial antibody titre of the babies' sera. This relationship could be described by a rectangular hyperbola. Babies with high antibody titres at birth lost this antibody rapidly whereas in babies with low initial titres antibody declined over a longer period.The half-lives of parainfluenza type 3 antibody and influenza A 2 antibody were compared with that of rubella antibody in the same group of infants (previously published). Maternally acquired viral antibodies of different specificities did not necessarily decline at similar rates in any given child. In nine infants, maternally acquired antibodies of two different specificities (rubella and parainfluenza type 3) declined at significantly different rates in the same child. It is suggested that although the half-life of antibody of a given specificity is related to its concentration in the serum, it is independent of the level of serum antibodies of other specificities.

scholarly journals Sex-Change Chemicals and their Influence on the Brain

2001 ◽  
Vol 1 ◽  
pp. 681-683 ◽  
Author(s):  
Catherine A. Harris
Keyword(s):  
Endocrine Disrupting Chemicals ◽  
Reproductive Disorders ◽  
Developmental Abnormalities ◽  
Scientific World ◽  
Endocrine Disrupting ◽  
Causative Agents ◽  
Wide Variability ◽  
Wildlife Populations ◽  
Natural Hormones ◽  
The Brain

The potential for man-made chemicals to mimic or antagonise natural hormones is a controversial issue, but one for which increasing amounts of evidence are being gathered worldwide. The controversy surrounds not so much the matter of whether these chemicals can mimic hormones invitro— this phenomenon has been widely accepted in the scientific world — but more whether, as a result, they can disrupt reproduction in a wildlife situation. It has, nevertheless, been acknowledged that many wildlife populations are exhibiting reproductive and/or developmental abnormalities such as intersex gonads in wild roach populations in the U.K.[1] and various reproductive disorders in alligators in Lake Apopka, Florida[2]. However, the causative agents for many of these effects are difficult to specify, due to the extensive mixtures of chemicals — each of which may act via different pathways — to which wild populations are exposed, together with the wide variability observed even in natural (uncontaminated) habitats. As a result, any information detailing fundamental mechanism of action of the so-called endocrine disrupting chemicals (EDCs) is of use in determining whether or not these chemicals, as they are present in the environment, may in fact be capable of causing some of the effects observed in wildlife over recent years.

scholarly journals Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris

2011 ◽  
Vol 20 (2) ◽  
pp. 103-110 ◽  
Author(s):  
Rodrigo Casquero Cunha ◽  
Renato Andreotti ◽  
Fábio Pereira Leivas Leite
Keyword(s):  
Pichia Pastoris ◽  
Boophilus Microplus ◽  
Economic Losses ◽  
Cattle Tick ◽  
High Antibody ◽  
Western Blot Assay ◽  
Development Of Resistance ◽  
Cattle Ticks ◽  
Antibody Titers

The cattle tick Rhipicephalus (Boophilus) microplus is responsible for great economic losses. It is mainly controlled chemically, with limitations regarding development of resistance to the chemicals. Vaccines may help control this parasite, thereby reducing tick pesticide use. In this light, we performed subcloning of the gene of the protein Bm86-GC, the homologue protein that currently forms the basis of vaccines (GavacTM and TickGardPLUS) that have been developed against cattle ticks. The subcloning was done in the pPIC9 expression vector, for transformation in the yeast Pichia pastoris. This protein was characterized by expression of the recombinant Mut+ strain, which expressed greater quantities of protein. The expressed protein (rBm86-CG) was recognized in the Western-blot assay using anti-Gavac, anti-TickGard, anti-larval extract and anti-rBm86-CG polyclonal sera. The serum produced in cattle vaccinated with the antigen CG rBm86 presented high antibody titers and recognized the native protein. The rBm86-GC has potential relevance as an immunogen for vaccine formulation against cattle ticks.

scholarly journals Staphylococcus aureus Exotoxins and Their Detection in the Dairy Industry and Mastitis

Toxins ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 537 ◽  
Author(s):  
Ana G. Abril ◽  
Tomás G. Villa ◽  
Jorge Barros-Velázquez ◽  
Benito Cañas ◽  
Angeles Sánchez-Pérez ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Industry ◽  
Food Poisoning ◽  
Microbial Colonization ◽  
Economic Losses ◽  
Human Consumption ◽  
Negative Effects ◽  
Virulence Proteins ◽  
Causative Agents ◽  
Best Medical Therapy

Staphylococcus aureus constitutes a major food-borne pathogen, as well as one of the main causative agents of mastitis in dairy ruminants. This pathogen can produce a variety of extracellular toxins; these include the shock syndrome toxin 1 (TSST-1), exfoliative toxins, staphylococcal enterotoxins (SE), hemolysins, and leukocidins. S. aureus expresses many virulence proteins, involved in evading the host defenses, hence facilitating microbial colonization of the mammary glands of the animals. In addition, S. aureus exotoxins play a role in the development of both skin infections and mastitis. Indeed, if these toxins remain in dairy products for human consumption, they can cause staphylococcal food poisoning (SFP) outbreaks. As a result, there is a need for procedures to identify the presence of exotoxins in human food, and the methods used must be fast, sensitive, reliable, and accurate. It is also essential to determine the best medical therapy for human patients suffering from S. aureus infections, as well as establishing the relevant veterinary treatment for infected ruminants, to avoid economic losses in the dairy industry. This review summarizes the role of S. aureus toxins in the development of mastitis in ruminants, their negative effects in the food and dairy industries, and the different methods used for the identification of these toxins in food destined for human consumption.

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