scholarly journals INVESTIGATION OF PRESENCE OF METHICILLIN RESISTANT STAPHYLOCOCCI IN STUDENTS OF THE FACULTY OF VETERINARY MEDICINE AT THE UNIVERSITY OF BELGRADE

2017 ◽  
Vol 9 (2) ◽  
pp. 17-28
Author(s):  
Milan Rađenović ◽  
Jelena Ašanin ◽  
Ksenija Aksentijević ◽  
Dušan Mišić
Keyword(s):  
Veterinary Medicine ◽  
Fatal Outcome ◽  
Meca Gene ◽  
Human Populations ◽  
Methicillin Resistant ◽  
Axillary Region ◽  
The University ◽  
Systemic Infections ◽  
Bacterial Resistances ◽  
Research Show

Resistance to methicillin in staphylococci is considered to be one of the most dangerous forms of bacterial resistances to antibiotics. Methicillinresistant staphylococci (MRS) are zoonotic agents which cause local and systemic infections in humans and animals, oft en with a fatal outcome due to the absence of adequate antibiotic therapy. People colonized with strains of MRS are asymptomatic carriers and reservoirs of these strains in human populations. Th e aim of this research was to determine the prevalence of strains of MRS among clinically healthy students of the Faculty of Veterinary Medicine in Belgrade. Th e study was conducted on 100 volunteers: 62 males and 38 females. Given that staphylococci are expected to be found in the highest percentage in the nose and on the armpit skin, the swabs were taken from these regions of each person. Blood agar was innoculated immediately on taking the swabs Aft er the incubation and isolation, the staphylococci were identifi ed to species level. Their susceptibility to methicillin was tested in a disk-diff usion test with cefoxitin. All strains which were found to be resistant to cefoxitin were investigated for the presence of mecA gene with PCR. Staphylococci were isolated in 146 out of the 200 swabs taken: there were 79 nose swabs and 67 axillar swabs positive for these bacteria. Seventeen isolates were resistant to cefoxitin and the presence of the mecA gene was confi rmed in seven, four of which were taken from the nose and three from the axillary region. The results of this research show that, being 6%, the prevalence of mecA-positive staphylococci in the population of clinically healthy students of veterinary medicine is significant. Th e percentage of methicillin-resistant staphylococci was higher in nose than in the axillar region of the students.

scholarly journals Bacteriological and Molecular Detection of Methicillin-resistant Staphylococcus Aureus Isolated From Cigarette and Hookah Smokers in Khartoum State

2020 ◽  
Author(s):  
Jara Osman Ali Osman ◽  
Esraa abdulraman Abdallah Bakri ◽  
Ahmed Ibahim Hashim ◽  
Ahmed Bakheet Abd Alla ◽  
Hisham N Altayb ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Detection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Meca Gene ◽  
Traditional Culture ◽  
Culture Methods ◽  
Methicillin Resistant ◽  
Systemic Infections

Abstract Objective Staphylococcus aureus is an extra-ordinarily versatile pathogen that causes a wide variety of infections in humans and animals, capable of causing superficial lesions and systemic infections. The aim of this study was to detect mecA gene in smokers of cigarette and hookah. Results Forty-one isolates (41%) were identified as S. aureus by traditional culture methods. non-smokers, nasal carriers of S. aureus were 16 (39%) while 25 (60%) among smokers (P = 0.103). Of the overall 25 smoker nasal carriers, 18 were hookah smokers and 7 were cigarettes smokers (P = 0.004). MRSA was 1 (2.4%) in non-smokers and 3 (7.2%) in smokers, 2 in cigarettes and 1 in hookah (P = 0.009). Only 38 isolates were confirmed as S. aureus by PCR (mecA gene). In non-smokers group mecA were 6 (15.8%) positive and 8 (21%) negative, while in smokers were 16 (42.3%) positive and 8 (20.9%) negative isolates (P = 0.187). From hookah 12 mecA positive and 4 from cigarette (P = 0.647). The correlation of MRSA and using of antibiotic during last 6 months indicates significant (P = 0.031).

Microarray Technology for the Detection of Antimicrobial Resistance Genes Present in Hospital and Community Acquired Methicillin Resistant S.aureus Strains

2017 ◽  
Vol 68 (11) ◽  
pp. 2546-2550
Author(s):  
Monica Licker ◽  
Andrei Anghel ◽  
Roxana Moldovan ◽  
Elena Hogea ◽  
Delia Muntean ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Dna Microarray ◽  
Bacterial Resistance ◽  
Meca Gene ◽  
Modern Medicine ◽  
Microarray Technology ◽  
Methicillin Resistant ◽  
Antimicrobial Sensitivity ◽  
Antimicrobial Resistance Genes ◽  
Mrsa Strains

Antimicrobial resistance (AMR) represents a real burden for the modern medicine. One of the most frecvently isolated hospital acquired (HA) pathogens wordlwide, is Methicillin resistant Staphylococcus aureus (MRSA). Recently not only HA, but also community-acquired MRSA (CA-MRSA) infections have been reported. A prospective study was performed between February 2009 and October 2010, with the aim to investigate bacterial resistance of CA-MRSA and HA-MRSA. DNA microarray technology has been used for the detection of 4 AMR genes for the studied MRSA strains. A number of 218 HA- S.aureus strains have been isolated, from which 89 (40. 82%) were MRSA. In the community, 1.553 S.aureus strains were isolated, out of which, 356 (22. 92%) were MRSA. From these, a number of 17 HA and 12 CA �MRSA strains have been analyzed by DNA microarray technology. From 100% phenotypically described HA- MRSA, we identified mecA gene in 10 strains (58. 83%). Other 6 strains (35. 29%) have been erm(A) positive and 4 (23. 53%) - tet(O) positive. 83. 33% (10 strains) from the CA strains had mecA gene, only one (8. 33%) was erm(A) positive and 4 (33. 33%) were erm(C) positive. DNA microarray is a method allowing the concomitant scan of multiple genes and can be done within a few hours. That type of rapid and reliable methods for antimicrobial sensitivity tests are important to start an appropriate therapy.

scholarly journals Mutation-Based Antibiotic Resistance Mechanism in Methicillin-Resistant Staphylococcus aureus Clinical Isolates

2021 ◽  
Vol 14 (5) ◽  
pp. 420
Author(s):  
Tanveer Ali ◽  
Abdul Basit ◽  
Asad Mustafa Karim ◽  
Jung-Hun Lee ◽  
Jeong-Ho Jeon ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Active Site ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Meca Gene ◽  
Resistance Mechanism ◽  
Ligand Docking ◽  
Clinical Samples ◽  
Allosteric Site ◽  
Methicillin Resistant

β-Lactam antibiotics target penicillin-binding proteins and inhibit the synthesis of peptidoglycan, a crucial step in cell wall biosynthesis. Staphylococcus aureus acquires resistance against β-lactam antibiotics by producing a penicillin-binding protein 2a (PBP2a), encoded by the mecA gene. PBP2a participates in peptidoglycan biosynthesis and exhibits a poor affinity towards β-lactam antibiotics. The current study was performed to determine the diversity and the role of missense mutations of PBP2a in the antibiotic resistance mechanism. The methicillin-resistant Staphylococcus aureus (MRSA) isolates from clinical samples were identified using phenotypic and genotypic techniques. The highest frequency (60%, 18 out of 30) of MRSA was observed in wound specimens. Sequence variation analysis of the mecA gene showed four amino acid substitutions (i.e., E239K, E239R, G246E, and E447K). The E239R mutation was found to be novel. The protein-ligand docking results showed that the E239R mutation in the allosteric site of PBP2a induces conformational changes in the active site and, thus, hinders its interaction with cefoxitin. Therefore, the present report indicates that mutation in the allosteric site of PBP2a provides a more closed active site conformation than wide-type PBP2a and then causes the high-level resistance to cefoxitin.

scholarly journals Antibiotic Resistance Profile and Biofilm Production of Staphylococcus pseudintermedius Isolated from Dogs in Thailand

2021 ◽  
Vol 14 (6) ◽  
pp. 592
Author(s):  
Pavarish Jantorn ◽  
Hawaree Heemmamad ◽  
Tanawan Soimala ◽  
Saowakon Indoung ◽  
Jongkon Saising ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Meca Gene ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Antibiotic Resistance Profile ◽  
Biofilm Production ◽  
Southern Thailand ◽  
Life Threatening ◽  
Suitable Treatment ◽  
Resistant Strains

Staphylococcus pseudintermedius is a zoonotic pathogen that can cause life-threatening infections in animals and humans. The study of methicillin-resistant S. pseudintermedius (MRSP) and its ability to produce biofilms is important to select the most suitable treatment. The prevalence and characteristics of S. pseudintermedius isolated from dogs admitted at the Veterinary Teaching Hospital, Prince of Songkla University, Thailand were assessed. Results showed that 28.30% (15/53) of the isolates were MRSP. Amplification of the mecA gene was observed in 93.33% (14/15) MRSP. Methicillin-resistant strains revealed co-resistant patterns against other antibiotics, including chloramphenicol, clindamycin, tetracycline, clarithromycin, ciprofloxacin, and trimethoprim. In this study, all bacterial isolates produced biofilms, while 90.55% of S. pseudintermedius isolates were strong or moderate biofilm producers. Most (45–60%) of the resistant strains were strong biofilm producers, while the correlation between biofilm production and antibiotic resistance was not statistically significant. This is the first study in southern Thailand to investigate the drug-resistant profile of S. pseudintermedius and its ability to form biofilm. The results will contribute to a better understanding of the emergence and prevalence of antimicrobial resistance in S. pseudintermedius.

scholarly journals Multiple Antimicrobial Resistance in Methicillin-Resistant Staphylococcus sciuri Group Isolates from Wild Ungulates in Spain

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 920
Author(s):  
Joaquín Rey Pérez ◽  
Laura Zálama Rosa ◽  
Alfredo García Sánchez ◽  
Javier Hermoso de Mendoza Salcedo ◽  
Juan Manuel Alonso Rodríguez ◽  
...  
Keyword(s):  
Cervus Elaphus ◽  
Methicillin Resistance ◽  
Bacterial Species ◽  
Sccmec Type ◽  
Meca Gene ◽  
Domestic Animal ◽  
Wild Ungulates ◽  
Methicillin Resistant ◽  
Phenotypic Resistance ◽  
Columbia Blood Agar

The aim of this study was to investigate the presence of methicillin-resistant Staphylococcus (MRS) strains in non-managed wild ungulates present in a typical Mediterranean forest in Spain. For this purpose, nasal swabs were obtained from 139 animals: 90 wild boar (Sus scrofa), 42 red deer (Cervus elaphus) and 7 fallow deer (Dama dama), which were subsequently pre-enriched in BHI+ NaCl (6.5%) (24 h/37 °C), and then seeded in Columbia blood agar (24 h/37 °C)). The presence of the mecA gene was investigated by PCR, first from the confluent and then from individual colonies. A total of 10 mecA+ colonies were obtained of which only seven showed phenotypic resistance to oxacillin/cefoxitin (methicillin resistance). All MRS strains belonged to the Staphylococcus sciuri group. Methicillin-resistant Staphylococcus aureus (MRSA) was not detected. In addition, a significant number of MRS strains showed resistance to other antimicrobials, mainly β-lactam (7/7), gentamicin (7/7), fusidic acid (6/7) and quinupristin–dalfopristin (6/7), showing an irregular correlation with their coding genes. The genetic profiles grouped the seven strains obtained according to the bacterial species but not in relation to the animal source or the geographical place of origin. The presence of SCCmec type III, common to animals and humans, has been detected in three of the strains obtained. In conclusion, the study reveals that the wild ungulates investigated play a role as potential reservoirs of multi-resistant strains of MRS. Such strains, due to their characteristics, can be easily transferred to other wild or domestic animal species and ultimately to humans through their products.

FOALS ARE GETTING HELP

PEDIATRICS ◽  
1989 ◽  
Vol 84 (4) ◽  
pp. A96-A96
Author(s):  
J. F. L.
Keyword(s):  
Intensive Care Unit ◽  
Veterinary Medicine ◽  
Intensive Care ◽  
Birth Defects ◽  
Human Medicine ◽  
University Of Pennsylvania ◽  
Twin Brother ◽  
Pennsylvania Hospital ◽  
The University

KENNETT SQUARE, Pa.—Nearly as rare as the colt that grows up to be a racing champion is the birth of twin foals. Yet a tiny and brave filly and her weaker twin brother grow stronger every day here in an intensive care unit for newborn horses. Established in 1983 and directed . . . by Dr. Wendy E. Vaala, a . . . veterinarian, the University of Pennsylvania's intensive care unit for foals was built. . . . It is one of only seven such units in the country, and they have led to the development of a new specialty in veterinary medicine—equine neonatology. Recipes for formula fed to foals were borrowed from those used at the University of Pennsylvania Hospital in Philadelphia. The intensive care unit uses ultrasound equipment, heart monitors and other devices commonly used in human neonatal medicine. Treatments for infections, poisoning, ulcers, birth defects, even difficult births were adopted from human medicine. . . . But there are no incubators. . . .The foals are too active.

scholarly journals Prevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyoderma

2016 ◽  
Vol 36 (12) ◽  
pp. 1178-1180 ◽  
Author(s):  
Larissa S. Botoni ◽  
Carolina B. Scherer ◽  
Rodrigo O. Silva ◽  
Fernanda M. Coura ◽  
Marcos B. Heinemann ◽  
...  
Keyword(s):  
Meca Gene ◽  
Skin Lesions ◽  
In Vitro Susceptibility ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Oxacillin Resistance ◽  
Gene Carriers ◽  
Genotypic Characteristics

ABSTRACT: In order to assess the prevalence of Methicillin-resistant Staphylococcus pseudintermedius from skin and nostrils of dogs with pyoderma, to determine its in vitro susceptibility, and to correlate these data with the presence of the mecA gene, 43 dogs were selected. Samples were collected from secretion of their skin lesions and right nostril, cultured, and analyzed for phenotypic and genotypic characteristics of the bacteria studied. In 62 samples (91%) the microorganism was classified as S. pseudintermedius. The rate of resistance against antibiotics ranged from 7% (amikacin; 4/62) to 77% (sulfamethoxazole + trimethoprim; 48/62). Resistance against oxacillin was found in 34% of the samples (21/62). Twenty-five samples (37%) were strains that carried the mecA gene. A significant correlation (P<0.01) was found between presence of the mecA gene and oxacillin resistance. Seventeen dogs were mecA gene carriers, and 8 (47%) of them had the gene in the skin lesions and nostril. A significant correlation (P<0.01) was also observed between the presence of mecA gene in the skin lesions and nostrils. Oxacillin resistance in vitro can be safely used to indicate the presence of mecA gene in MRSP samples. The nostrils can be a reservoir of MRSP in dogs.

scholarly journals Internationalization at the University of São Paulo

2016 ◽  
Vol 95 (spe3) ◽  
pp. 5
Author(s):  
Raul Machado Neto
Keyword(s):  
Veterinary Medicine ◽  
Medical School ◽  
Sao Paulo ◽  
Law School ◽  
Human Sciences ◽  
São Paulo ◽  
College Of Agriculture ◽  
Engineering School ◽  
The University

The University of São Paulo, founded in 1934, started under the influence of important foreigners academicians in our campuses. The beginning of our university was the result of a fusion of the already existing colleges – Law School, School of Engineering, School of Pharmacy and Dentistry, College of Agriculture, Medical School, and School of Veterinary Medicine. In addition, in 1934, the School of Philosophy, Sciences and Letters was created being responsible for human sciences – Philosophy, History, Geography, Sociology – and hard sciences – Mathematics, Physics, and Chemistry – that academically amalgamated the professional existing colleges. In the thirties, we benefited from the instabilities in Europe and important professors came to the University of São Paulo contributing remarkably to our successful trajectory.[...]

scholarly journals The Impact of Characteristics of Immigrant Offenders on Attitudes Towards Immigrant Crime

2021 ◽  
Vol 30 (1) ◽  
pp. 51-71
Author(s):  
Mateja Vuk ◽  
Dalibor Doležal ◽  
Ena Jovanović
Keyword(s):  
Public Attitudes ◽  
Significant Proportion ◽  
Punitive Attitudes ◽  
Negative Attitudes ◽  
The Public ◽  
Minority Threat ◽  
The University ◽  
The Impact ◽  
Threat Theory ◽  
Research Show

Minority threat theory and existing research show that public attitudes towards certain types of offenders (e.g. ethnic and racial minorities) are often more punitive. Research also reveals that a significant proportion of the public associates the increase of immigration with higher crime rates. Negative attitudes, as well as an overall anti-immigration sentiment, have been increasing internationally. Therefore, we hypothesise that the public will have more negative and punitive attitudes towards immigrant offenders than towards citizens. Using a sample of students from the University of Zagreb, this research tested the above-mentioned hypothesis and explored whether factors like immigration status, ethnic identity, type of offense, and the age of the hypothetical offender impact student attitude on immigrant crime. To test this proposition, we used online surveys with factorial vignettes. The results show that participants ask for harsher sentences for undocumented immigrants, but immigrant status and the national origin of the immigrant are not associated with the perception of recidivism, dangerousness, or criminal typicality of offender.

scholarly journals Detection of mecA gene by latex agglutination and its molecular analysis by PCR in methicillin resistant staphylococcus aureus.

2020 ◽  
Vol 27 (07) ◽  
pp. 1363-1370
Author(s):  
Aneela Khawaja ◽  
Iffat Javed ◽  
Sohaila Mushtaq ◽  
Saeed Anwar ◽  
Faiqa Arshad ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Latex Agglutination ◽  
Medical Institute ◽  
Cross Sectional ◽  
Methicillin Resistant ◽  
Agglutination Method ◽  
Resistant Strains

Antimicrobial resistance (AMR) is a devastating question that is threatening the health globally. The extensive and indiscriminative use of antibiotics has evolved a notorious resistance in Staphylococcus aureus.  This resistance developed through possession of mecA gene, which codes for modified penicillin binding protein (PBP2a) and the emergent strain being labeled “methicillin resistant Staphylococcus aureus”. Conventional phenotypic techniques for detection of MRSA rely on standardization of cultural characteristics. The drawbacks of diagnostic error to report MRSA include: poor prognosis, expensive treatment, dissemination of multi-drug resistant strains and even treatment failure. Latex agglutination method can be adopted as a more accurate and quick strategy for rapid detection of methicillin resistance. Objectives: To compare detection of mecA gene in methicillin resistant isolates of Staphylococcus aureus by latex agglutination and PCR; by assessing the sensitivity and specificity of both methods. Study Design: Descriptive Cross-Sectional study. Setting: Pathology Department, Post Graduate Medical Institute, Lahore. Period: From January 2015 to December 2015; according to standard operating procedures at Microbiology laboratory. Material & Methods: A total 713 consecutive, non-duplicate isolates of Staphylococcus aureus were processed. Methicillin resistance was determined using cefoxitin (30mg) by Kirby-Bauer method using CLSI guideline (2016), latex agglutination method; and PCR for mecA gene. Results: The results showed that out of 713 Staphylococcus aureus isolates, 92 (12.90%) isolates were resistant to cefoxitin and were labelled as MRSA. majority MRSA isolates recovered from pus (44.57%) and wound swab (20.65%), followed by blood (13.04%), fluid (8.70%), CSF (4.35%), CVP (3.26%), HVS (3.26%) and tracheal secretion (2.17%). By latex agglutination method, 87 (94.50%) were positive for PBP2a; while on PCR mecA gene was detected only in 82 (89.10%) MRSA isolates. When assessed with PCR (gold standard) the sensitivity and diagnostic accuracy of latex agglutination was 100% and 94.57%, respectively. Conclusion: Latex agglutination test can be employed as rapid and reliable diagnostic technique in MRSA isolates for mecA gene detection, where resources for molecular methods are inadequate. This can effectually lessen the misdiagnosis of resistant strains, and over/ ill-use of antibiotics.

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