Pharmacognostical and Phytochemical Analysis of Rasayana Churna Vati for Quality Control - An Ayurveda Formulation

Rasayana churna was mentioned by Acharya Vagbhatta for rejuvenation of body tissue. Rasayana properties of this combination revitalize the unhealthy tissue on systemic level. Rasayana Churna is a polyherbal formulation contacting 3 ingredients i.e., Amalaki, Guduchi and Gokshura are well known drugs for its rich in antioxidant, immunomodulator and purifactory action. Repeated stress injury in visual system is major cause of Computer Vision Syndrome- a new milliner disorder. A study conducted as randomized clinical trial showed that Rasayan churana significantly improves a group of symptoms in Computer Vision Syndrome. The present study was carried out to standardize the finished product of Rasayana Churna Vati. It includes Authentication, Organoleptic properties, physic-chemical parameters, assay of active and qualitative HPTLC analysis to confirm entity, quality and purity. All results were compared with Ayurvedic Pharmacopoeial standard. The presence of rosette crystals of calcium oxalate, lignified tissues, prismatic crystals, reticulate vessels, starch grains oval, tannins, trichomes were the characteristic features observed in the microscopy of the prepared drug. Phyto-chemical analysis showed Loss on drying 1.87% w/w, Total ash 4.35% w/w, water soluble extract 34.73% w/w, Acid insoluble ash 0.73% w/w, Alcohol soluble extractive 14.34% w/w & pH 3.4. Identification of constituents of Rasayana Churna Vati was done by HPTLC scanning at 254 and 366mm gives positive test for Guduchi, Gokshura and Amalaki.

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PHARMACOGNOSTICAL, PHYSICOCHEMICAL AND PHYTOCHEMICAL STUDIES OF PIPER LONGUM LINN. FRUITS

INTERNATIONAL JOURNAL OF PHARMACEUTICAL EDUCATION AND RESEARCH (IJPER) ◽

10.37021/ijper.v1i1.15 ◽

2019 ◽

Vol 1 (1) ◽

pp. 19-24

Author(s):

Priyanka Sharma

Keyword(s):

Chemical Constituents ◽

Volatile Oil ◽

Water Soluble ◽

Phytochemical Analysis ◽

Chemical Parameters ◽

Piper Longum ◽

The Family ◽

Perennial Shrub ◽

Physical And Chemical ◽

Phytochemical Studies

Introduction: Piper longum Linn, belonging to the family Piperaceae, is a climber, perennial shrub which is commonly found in India. The dried fruits of Piper longum are widely used in Ayurvedic System of medicines since time unknown. It posses significant pharmacological properties due to presence of variety of chemical constituents in it. Objective: The present study is aimed to evaluate the pharmacognostical, physiochemical and phytochemical parameters for Piper longum fruits, as per the WHO guidelines for herbal drug standardization. Materials and Methods: WHO emphasized the use of standardized herbs and formulations for safety and best therapeutic results. Results and Discussion: Pharmacognostical studies shows that the fruits of Piper longum are found as green when fresh which turns grayish black upon drying. It posses pungent, bitter and acrid taste. The fruits are cylindrical with small petiole. The powder microscopy shows the presence of brown content, oleo resins, stone cells and calcium oxalate crystals. Physiochemical analysis shows variable extractive values in different solvents with maximum extractives of 20.6±0.021% in water and minimum extractive value of 6.6±0.036% in chloroform. Total Ash value of 8.3±0.015%, Acid Insoluble Ash- 1.16±0.025% and Water Soluble Ash- 5.5±0.01%, Foreign Matter- 1.62±0.12%, Moisture content of 15.70±0.051%, Bitterness value-1.96, Swelling Index-1.5±0.01 and Foaming Index- 102.33. Phytochemical analysis of the fruits of Piper longum shows the presence of alkaloids, volatile oil, tannins, fixed oils, and phenolics. Conclusion: The botanical, physical and chemical parameters obtained in this study can be used for establishing the identity and purity of the drug that will lead to safety and efficacy of the herb.

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AN OPEN LABELLED RANDOMISED CLINICAL TRIAL ON EFFICACY OF HARITAKI MODAKA AND ABHIJEET TAILA IN THE MANAGEMENT OF COMPUTER VISION SYNDROME

International Journal of Ayurveda and Pharma Research ◽

10.47070/ijapr.v8i8.1549 ◽

2020 ◽

pp. 15-20

Author(s):

Akanksha Thakur ◽

Manjusha Rajagopala ◽

Narayan Bavalatti ◽

Pankaj Kundal

Keyword(s):

Computer Vision ◽

Medical Science ◽

Randomised Clinical Trial ◽

Progressive Increase ◽

Blurred Vision ◽

Stress Injury ◽

Computer Vision Syndrome ◽

Group A ◽

Systemic Symptoms ◽

Group B

Computer Vision Syndrome is the new nomenclature to the visual, ocular, and systemic symptoms arising due to the long time and improper working on the computer. Computer Vision Syndrome is a repetitive strain disorder as defined by the American Optometric. With progressive increase in the usage of computers, the term Repeated Stress Injury has found a place in contemporary medical science. The ocular complaints experienced by computer users typically include eyestrain, eye fatigue, burning sensations, irritation, redness, blurred vision, and dry eyes, among others. This group of symptoms is called Asthenopia. The incidence of Computer Vision Syndrome is as high as 50% to 90% among the employees of computer occupation. The causes for the inefficiencies and the visual symptoms are a combination of individual visual problems and poor office ergonomics. Material & Methods: In this clinical study on CVS, 60 patients were registered from Shalakya tantra OPD, and all of them completed the treatment. In Group A, 30 patients were prescribed Abhijeet Taila for Pratimarsha Nasya and in Group B, 30 patients were prescribed Haritaki Modak tablets internally and Abhijeet Taila for Pratimarsha Nasya. Results: In total, 20% and 33.33% patients got completely cured in Group A and B respectively. Discussion: Both the drugs were effective in managing the condition, but it was observed they were more effective when given together. Multi-centric studies with larger sample size on the same drugs should be carried out to evaluate the effect of the drugs and, also so that the observations can be noted well and can be better suggested.

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The PHARMACOGNOSTICAL, PHYSICOCHEMICAL AND HIGH-PERFORMANCE THIN LAYER CHROMATOGRAHY (HPTLC) EVALUATION OF ERANDAMULADI KALKA CHOORNA

Journal of Biomedical and Pharmaceutical Research ◽

10.32553/jbpr.v9i5.805 ◽

2020 ◽

Vol 9 (5) ◽

Author(s):

Swathi N. ◽

Anup B. Thakar ◽

Harisha C.R. ◽

V.J. Shukla

Keyword(s):

Pharmaceutical Analysis ◽

High Performance ◽

Water Soluble ◽

Phytochemical Analysis ◽

Low Back ◽

Soluble Extract ◽

Physico Chemical ◽

Common Ailment ◽

Back Ache ◽

Water Soluble Extract

Introduction: Erandamuladi Niruha Basti is mentioned in Charaka Samhita which is indicated in Trika, Prishta Shoola (low back ache) and acts as Maruta Nigraha. Low backache is the most common ailment in today’s busy life. Erandamuladi Kalka used in Basti contains 9 drugs in which most of the drugs are having Ushna Veerya and are Shoolahara in nature. Method: Sookshma Choorna of Erandamuladi Kalka was evaluated for their pharmacognostic and pharmaceutical analysis. Results: Pharmacognostic study showed the presence of contents such as cluster crystal of Hapusha, starch grains of Musta, spiral vessels of Bala etc. Physico-chemical analysis showed that the loss on drying 6.28%, Ash value 13.26%, Water soluble extract 19.34%, Alcohol soluble extract 4.24%, pH 7.0 Discussion and Conclusion: The pharmacognostical and phytochemical analysis of Erandamuladi Kalka Choorna confirmed the purity and genuinity of drug, which can be espoused for laying down the standards of it. Keywords: Erandamuladi Kalka Choorna, Pharmacognostic, Phytochemical analysis, HPTLC

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PHARMACOGNOSTICAL, PHYSICOCHEMICAL AND HPTLC EVALUATION OF AMRUTADHYA GUGGULU A POLYHERBAL FORMULATION FOR PHARMACEUTICAL STANDARDIZATION

Journal of Biomedical and Pharmaceutical Research ◽

10.32553/jbpr.v9i1.721 ◽

2020 ◽

Vol 9 (1) ◽

Author(s):

Divya Zala ◽

Dilip Prajapati ◽

Anup Thakar ◽

Harisha CR ◽

V.J.Shukla

Keyword(s):

Pharmaceutical Analysis ◽

Quality Standard ◽

Water Soluble ◽

Soluble Extract ◽

Traditional Medicines ◽

Polyherbal Formulation ◽

Characteristic Features ◽

Increasing Demand ◽

Water Soluble Extract

Background: Standardization of herbal formulation is needed in order to assess of quality of drug. Maintaining the quality standard of a polyherbal formulation is a difficult task. In the era of increasing demand for traditional medicines, maintaining quality standards is the need of the hour. Aim: The present work was carried out to standardize the finished product Amrutadhya Guggulu to confirm its identity, quality and purity. Material and Method: Amrutadhya Guggulu is mentioned in the treatment of Sthaulya (Obesity), and it was prepared according to the method as described in Chakradatta. Amrutadhya Guggulu powder was evaluated for its pharmacognostic and pharmaceutical analysis. It contains drug like Guduchi, Ela, Kutaja Phala, Kutaj twaka etc. Result: Epicarp and stone cell of Vidanga, silica deposition of Amalaki, oil globule of Ela etc were the characteristic features observed in the microscopy of Amrutadhya Guggulu powder .Results found in pharmaceutical parameters of Amrutadhya Guggulu powder like Loss on drying8.07% w/w, Ash value 3.07% w/w, Water soluble extract 22.5 % w/w, alcohol soluble extract 13.44%w/w and pH 4 Conclusion: The Obtained values of all parameters for the finish product can be adopted to set down new standards. Keywords: Amrutadhya Guggulu, HPTLC, Pharmacognostical, Pharmaceutical analysis

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Pharmacognostical and phytochemical standardization of Baladi churna- A polyherbal formulation

Indian Journal of Pharmaceutical and Biological Research ◽

10.30750/ijpbr.2.3.20 ◽

2014 ◽

Vol 2 (3) ◽

Author(s):

Anjali Verma ◽

LP Dei ◽

SB Donga ◽

Harisha CR ◽

Sarvesh Kumar

Keyword(s):

Quantitative Analysis ◽

Pharmaceutical Preparation ◽

Pollen Grains ◽

Water Soluble ◽

Implantation Failure ◽

Soluble Extract ◽

Polyherbal Formulation ◽

The World ◽

Characteristic Features ◽

Water Soluble Extract

Baladi Churna described by Acharya Bhavprkash in Vandhyatva (infertility). Endometrium dysfunction is the prime cause of Infertility among the world Baladi Churna is useful in patients especially having implantation failure due to defective endometrium, which is known as Kshetra deformity in Ayurveda. So a new pharmaceutical preparation Baladi churna was tried to standardize which is economical in terms of time and machinery usage. The present work was carried out to standardize the finished product of to confirm Baladi Churna entity, quality and purity. The presence of trichomes, spiral vessels, pollen grains were the characteristic features observed in the microscopy of the prepared drug. Phyto-chemical analysis showed Loss on drying 8.24% w/w, ash value 9 %w/w, water soluble extract 46.3 % w/w and pH 6.5. HPTLC of Baladi Churna is the preliminary quantitative analysis which shows 11 prominent spots in UV 254 nm and 10 prominent spots 366 nm. Baladi Churna, a polyherbal formulation of 6 ingredients was prepared and HPTLC finger print profile was developed and it can be considered pharmacopial standard of Baladi Churna.

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Pharmacognostical and phytochemical standardization of Baladi churna- A polyherbal formulation

Indian Journal of Pharmaceutical and Biological Research ◽

10.30750/ijpbr.2.3.19 ◽

2014 ◽

Vol 2 (03) ◽

pp. 126-131

Author(s):

Anjali Verma ◽

L. P. Dei ◽

S. B. Donga ◽

C. R. Harisha ◽

Sarvesh Kumar

Keyword(s):

Quantitative Analysis ◽

Pharmaceutical Preparation ◽

Pollen Grains ◽

Water Soluble ◽

Implantation Failure ◽

Soluble Extract ◽

Polyherbal Formulation ◽

The World ◽

Characteristic Features ◽

Water Soluble Extract

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Relationship between Computer Vision Syndrome and A Collection of Musculoskeletal Pain among Frequent Computer Users

Strengthening Hospital Competitiveness to Improve Patient Satisfaction and Better Health Outcomes ◽

10.26911/the6thicph.05.24 ◽

2019 ◽

Author(s):

Hari Peni Julianti ◽

◽

Trilaksana Nugroho ◽

Dea Amarilisa Adespin ◽

Arwinda Nugraheni ◽

...

Keyword(s):

Computer Vision ◽

Musculoskeletal Pain ◽

Computer Users ◽

Computer Vision Syndrome

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Study on Knowledge and Prevalence of Computer Vision Syndrome among Computer Operators in Nobel Medical College Teaching Hospital, Biratnagar, Nepal

Journal of Nobel Medical College ◽

10.3126/jonmc.v9i2.33386 ◽

2020 ◽

Vol 9 (2) ◽

pp. 45-49

Author(s):

Pramod Sharma Gautam ◽

Uday Chandra Prakash ◽

Subreena Dangol

Keyword(s):

Computer Vision ◽

Teaching Hospital ◽

Human Life ◽

College Teaching ◽

Extended Period ◽

Visual Display ◽

Work Place ◽

Medical College ◽

Computer Vision Syndrome ◽

Department Of Ophthalmology

Background: The eye and vision related problems that results from continuous use of computers and other visual display terminals for extended period of time leads to computer vision syndrome. Due to rapid digitalization in human life, the risk of developing it has also increased in many folds. So, with an aim of determining the prevalence and level of awareness of computer vision syndrome among computer users along with their attitude and practices to prevent it, this study was conducted in the office employees who use computer for a considerable period of time. Materials and Methods: A hospital based observational descriptive study was conducted in the out-patient department of Ophthalmology in Nobel Medical College and Teaching Hospital, Biratnagar, where 105 employees working in different work stations of same institution were enrolled. A questionnaire and the clinical findings were used to collect data. Results: About 80% of the employees were using computer for about (8-11) hours per day. Prevalence of computer vision syndrome noted was (92.4%) with low level of knowledge (85.7%) about it. About 45% of them wore glasses for their refractive errors but attitude and practices in work place to prevent the bad effects of using visual display terminals were found to be lacking (53.3%). Burning sensation in the eye, headache, ocular irritation and itching and neck, shoulder or back pain were the common symptoms. Around (60-70)% of the eyes tested positive for dry eye. Conclusion: Lack of awareness of computer vision syndrome and lack of personal protective measures were associated with its high level of prevalence.

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Pharmacognostical Standardization and Pharmacological Potential of Elaeocarpus ganitrus Leaves as an Antiulcer Agent

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2018.11.4.3 ◽

2018 ◽

Vol 11 (4) ◽

pp. 4162-4169

Author(s):

Mayank Kulshreshtha ◽

Manjul Pratap Singh

Keyword(s):

Skin Diseases ◽

Scientific Data ◽

Water Soluble ◽

Histopathological Analysis ◽

Phytochemical Analysis ◽

Dependent Manner ◽

Soluble Extract ◽

Biochemical Studies ◽

Pharmacological Potential ◽

Dose Dependent

Elaeocarpus ganitrus Roxb, (E. ganitrus) known as Rudraksha belongs to family- Eleocarpaceae. It has a reflecting position in Hinduism and Ayurveda whereas traditionally it has mentioned to cure various health problems like fever, skin diseases, mental problems, wound healing etc. The present study was designed to study the microscopic and macroscopic analysis, physiochemical parameters, quantitative microscopy, phytochemical screening of E. ganitrus leaves as per WHO guidelines and evaluate the antiulcer potential of aqueous extract of E. ganitrus (AEEG) and ethanolic extract of E. ganitrus (EEEG) at the doses of 200 mg/kg and 400 mg/kg using pylorus ligation induced ulcers model, biochemical parameters. Hepatic, cardiac, hematological parameters have also done to find out the effect of different extracts on other major organs. Microscopic analysis proved the presence of covering trichomes, upper epidermis, lower epidermis, stomata, phloem, xylem etc. Ash value, water soluble ash, acid soluble ash, water soluble extract, alcohol soluble extract, loss on drying, swelling index, foaming index found to be 4.3 ± 0.52, 0.2 ± 0.33, 2.0 ± 0.2, 13.7 ± 0.25, 12.5 ± 0.55, 9.8 ± 0.23, 3.6 ± 0.04, more than 100. Different quantitative parameters were found out. Phytochemical analysis of different extracts showed the presence of various primary and secondary metabolite like alkaloids, glycosides, tannin, phenolic compounds etc. Pharmacological potential showed that extracts treated, and sucralfate treated groups showed significantly decreases in ulcer index in all above-mentioned models, biochemical studies clearly showed significant decreases in volume, pH, free acidity, total acidity of gastric content and increases in gastric mucus parameters like protein, total hexoses, hexosamine, fucose, sialic acid and DNA level. The level of antioxidant enzymes like LPO (Lipid peroxidation), SOD (Superoxide dimutase) were decreased and CAT (Catalase) level was increased. Level of PC (Plasma corticosterone) was decreased. Hematological, hepatic, cardiac parameters found to be normal during extracts treatment. Histopathological analysis clearly supports the biochemical studies at various doses and it was found to be effective in dose dependent manner. The obtained scientific data may be helpful to prepare the monograph of the plant and E. ganitrus has antiulcer potential in a dose dependent. Detailed study needed for better exposure of plant.

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