scholarly journals Justification of the priority controlled sanitary-microbiological parameters to ensure the safety of hospital environment, medical organizations stationary type, regardless of their functional purpose

2020 ◽  
Vol 99 (4) ◽  
pp. 326-336
Author(s):  
С. М. Юдин ◽  
Н. В. Русаков ◽  
Анжелика Владимировна Загайнова ◽  
О. В. Грицюк ◽  
И. В. Курбатова ◽  
...  
Keyword(s):  
Ribosomal Proteins ◽  
Microbial Contamination ◽  
Hospital Environment ◽  
Laser Mass Spectrometry ◽  
Gram Positive ◽  
Diagnostic Laboratory ◽  
Environmental Objects ◽  
Microbiological Parameters ◽  
Stationary Type ◽  
Functional Purpose

Introduction. The work is devoted to evaluating the results of our own research of sanitary-microbiological monitoring of environmental objects in diversified treatment-and-prophylactic institutions of stationary type and of the analysis both of domestic and foreign data in order justify of the list of priority controlled sanitary-microbiological indices of air and surfaces to ensure the safety of hospital environment, medical organizations stationary type, regardless of their functional purpose. Material and methods. The survey was conducted in various premises in the medical-prophylactic institutions of stationary type for two years. Studies included determination of microbial contamination of the air environment, working surfaces, hands of personnel with the detection of bacteriological, virological, and mycological parameters, followed by macroscopic and microscopic identification of microorganisms and identification using automated systems with the method of time-of-flight matrix-assisted laser mass spectrometry platform MALDI-TOF, based on the study of the mass spectra of ribosomal proteins in the range of 1000-10000 Daltons and bioinformatic comparison of the obtained spectrum with database reference spectra and PCR. Results. As a result, the research of surface washings in the premises of a multidisciplinary clinic revealed microbial contamination with conditionally pathogenic gram-positive cocci, including S.aureus, gram-positive and gram-negative bacteria posing a serious epidemiological danger to patients in these wards regardless of the MOST profile and requires mandatory monitoring taking into account of the used disinfectants. Conclusion. Our own research and analysis of domestic and foreign literature showed that it is not enough to monitor the air in the MOST premises only in terms of total microbiological contamination. In the operating, procedural and dressing blocks, as well as in the wards, physiotherapeutic, diagnostic, laboratory rooms and auxiliary units, it is also necessary to take into account other sanitary and microbiological indices: total microbes count, gram-positive rods and cocci, including S.aureus, fungi, adeno-, entero-, astroviruses, coliphages.

Justification of the priority controlled sanitary-microbiological parameters to ensure the safety of hospital environment, medical organizations stationary type, regardless of their functional purpose

2020 ◽  
Vol 99 (4) ◽  
pp. 326-336
Author(s):  
S. M. Yudin ◽  
N. B. Rusakov ◽  
Anzhelika V. Zagainova ◽  
O. V. Gritsyuk ◽  
I. V. Kurbatova ◽  
...  
Keyword(s):  
Ribosomal Proteins ◽  
Microbial Contamination ◽  
Hospital Environment ◽  
Laser Mass Spectrometry ◽  
Gram Positive ◽  
Diagnostic Laboratory ◽  
Environmental Objects ◽  
Microbiological Parameters ◽  
Stationary Type ◽  
Functional Purpose

Introduction. The work is devoted to evaluating the results of our own research of sanitary-microbiological monitoring of environmental objects in diversified treatment-and-prophylactic institutions of stationary type and of the analysis both of domestic and foreign data in order justify of the list of priority controlled sanitary-microbiological indices of air and surfaces to ensure the safety of hospital environment, medical organizations stationary type, regardless of their functional purpose. Material and methods. The survey was conducted in various premises in the medical-prophylactic institutions of stationary type for two years. Studies included determination of microbial contamination of the air environment, working surfaces, hands of personnel with the detection of bacteriological, virological, and mycological parameters, followed by macroscopic and microscopic identification of microorganisms and identification using automated systems with the method of time-of-flight matrix-assisted laser mass spectrometry platform MALDI-TOF, based on the study of the mass spectra of ribosomal proteins in the range of 1000-10000 Daltons and bioinformatic comparison of the obtained spectrum with database reference spectra and PCR. Results. As a result, the research of surface washings in the premises of a multidisciplinary clinic revealed microbial contamination with conditionally pathogenic gram-positive cocci, including S.aureus, gram-positive and gram-negative bacteria posing a serious epidemiological danger to patients in these wards regardless of the MOST profile and requires mandatory monitoring taking into account of the used disinfectants. Conclusion. Our own research and analysis of domestic and foreign literature showed that it is not enough to monitor the air in the MOST premises only in terms of total microbiological contamination. In the operating, procedural and dressing blocks, as well as in the wards, physiotherapeutic, diagnostic, laboratory rooms and auxiliary units, it is also necessary to take into account other sanitary and microbiological indices: total microbes count, gram-positive rods and cocci, including S.aureus, fungi, adeno-, entero-, astroviruses, coliphages.

scholarly journals Microbes on the Mobile Phones of Healthcare Workers in Palestine: Identification, Characterization, and Comparison

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Mohammad Qadi ◽  
Rasha Khayyat ◽  
Mohammed A. AlHajhamad ◽  
Yazan I. Naji ◽  
Beesan Maraqa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Mobile Phone ◽  
Mobile Phones ◽  
Healthcare Workers ◽  
Microbial Contamination ◽  
Hospital Environment ◽  
Bacterial Isolates ◽  
Gram Positive ◽  
Gram Negative

Background. Healthcare workers (HCWs) may be using their mobile phones (MPs) to carry microbes that cause hospital-acquired and community infections in general. With antibiotic resistance problem emergence, these infections can be challenging to eradicate. Hence, this study aimed to determine the microbial contamination of HCW MPs and identify and classify bacterial isolates in Palestine. Methods. This was a 7-month comparative cross-sectional analysis of 200 HCW MPs from 2 hospitals and 100 MPs from university students (non-HCWs). Data collection was done using a self-administrated questionnaire, and a swab sample from both HCW and non-HCW MPs was obtained and transferred to An-Najah National University (NNU) microbiology lab for bacterial identification and antibiotic susceptibility. Data were analyzed using Social Sciences Statistical Package (SPSS) version 22.0. Result. Among HCWs, the microbial contamination was 87.5%. Coagulase-negative staphylococci (CoNS; 67.3%), methicillin-sensitive Staphylococcus aureus (MSSA; 17.5%), Gram-positive bacilli (4.1%), methicillin-resistant Staphylococcus aureus (MRSA; 1.6%), and Gram-negative species (1.6%) were the most predominant bacterial isolates. More than half of staphylococci isolates were resistant to penicillin and erythromycin. Male gender, using a mobile phone in the bathroom, and entry to the operating theatre were associated with mobile phone contamination and increased resistance against specific antibiotics. Among non-HCWs, the contamination was 86%. The most predominant bacterial isolates were CoNS, MSSA, and Gram-positive bacilli, with a contamination of 66.8%, 28.5%, and 2.6%, respectively. No MRSA or Gram-negative species were detected in this group. Antibiotic resistance percentage of staphylococci was nearly half of that yielded in the HCW group against each antibiotic. Conclusion. Significant numbers of bacteria have been isolated from HCW MPs. Working in a hospital environment frequently raises the probability of presence of antibiotic-resistant bacteria on a MP. Therefore, infection control teams should discuss methods to prevent the transmission of drug-resistant pathogens from HCW MPs.

scholarly journals PATIENTS' MICROFLORA COMPARATIVE CHARACTERISTICS OF DIFFERENT DEPARTMENTS AT THE NATIONAL HOSPITAL OF PEDIATRICS IN HANOI, VIETNAM

2019 ◽  
pp. 43-48
Author(s):  
L.V. Kataeva ◽  
A.P. Rebeshchenko ◽  
T.F. Stepanova ◽  
O.V. Posoiuznykh ◽  
Le Thanh Hai ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Infectious Diseases ◽  
Hospital Environment ◽  
Clinical Samples ◽  
Gram Negative Bacteria ◽  
National Hospital ◽  
Statistical Research ◽  
Gram Positive ◽  
Gram Negative

We studied the microflora structure and resistance gathered from the biomaterial of patients and the environment objects of various departments at the National hospital of Pediatrics in Hanoi. 140 clinical samples of biomaterials from 74 patients treated in the intensive care unit, the infectious diseases and the gastroenterology departments were studied. A systematic approach including microbiological, epidemiological and statistical research methods was used in carrying out the study. Bacteria of the Enterobacteriaceae family (38.5 per cent) prevailed in the biomaterial of intensive care unit patients. Nonfermentative Gram-negative bacteria (46.5 per cent) occupied the leading positions in the infectious diseases department and Gram-positive bacteria (39.3 per cent) were in the gastroenterology department. Gram-positive flora (60.2 per cent in the intensive care unit and 50.7 per cent in the infectious diseases department) prevailed in the microflora structure gathered from hospital environment objects. We identified the prevalence of bacteria of the genus Enterobacteriaceae and non-fermentative Gram-negative bacteria with a wide spectrum of resistance in the departments of the National Hospital of Pediatrics.

scholarly journals Infectious Risk of the Hospital Environment in the Center of Morocco: A Case of Care Unit Surfaces

Scientifica ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Samira Jaouhar ◽  
Abdelhakim El Ouali Lalami ◽  
Khadija Ouarrak ◽  
Jawad Bouzid ◽  
Mohammed Maoulouaa ◽  
...  
Keyword(s):  
Operating Room ◽  
Microbiological Quality ◽  
Hospital Environment ◽  
Coagulase Negative Staphylococci ◽  
Isolation And Identification ◽  
Gram Positive ◽  
Infectious Risk ◽  
Burn Unit ◽  
Risk Areas ◽  
Sterilization Service

Background. Equipment and hospital surfaces constitute a microbial reservoir that can contaminate hospital users and thus create an infectious risk. The aim of this work, which was carried out for the first time at a hospital in Meknes (regional hospital in the center of Morocco), is to evaluate the microbiological quality of surfaces and equipment in three potential risk areas (burn unit, operating room, and sterilization service). Methods. This study was carried out over a period of 4 months (February–May 2017). A total of 60 samples were taken by swabbing according to the standard (ISO/DIS 14698-1 (2004)) in an environment of dry area and equipment after biocleaning. Isolation and identification were performed according to conventional bacteriological methods and by microscopic observation for fungi. Results. The study showed that 40% of surface samples were contaminated after biocleaning. The burn unit recorded a percentage of 70% contamination (p value <0.001), 13% for the sterilization service, and 7% for the operating room. 89% of the isolates were identified as Gram-positive bacteria against 11% for fungi (p value <0.001). Bacterial identification showed coagulase-negative staphylococci (32%), Bacillus spp. (16%), Corynebacterium (8%), and oxidase-negative Gram-positive bacillus (40%) while fungal identification showed Aspergillus niger (n = 2) and Aspergillus nidulans (n = 1). Conclusion. To control the infectious risk related to equipment and hospital surfaces, it would be necessary to evaluate the disinfection protocol applied in these units.

Microbial Flora of Preseasoned Comminuted Turkey Meat

1977 ◽  
Vol 40 (5) ◽  
pp. 322-324 ◽  
Author(s):  
LINDA S. GUTHERTZ ◽  
JOHN T. FRUIN ◽  
JAMES L. FOWLER
Keyword(s):  
Escherichia Coli ◽  
Microbial Contamination ◽  
Microbial Flora ◽  
Plate Count ◽  
Gram Positive ◽  
Gram Negative ◽  
Turkey Meat ◽  
Standard Plate Count ◽  
Standard Plate

Preseasoned comminuted turkey meat, prepared at the retail level, was examined and revealed the following levels of microbial contamination per gram: mean standard plate count 2.2 × 108, mean coliform plate count 2.0 × 105, Escherichia coli count 8.7. Gram-positive and gram-negative microbial flora were isolated and identified.

scholarly journals Evaluation of physico-chemical and microbiological parameters of Jhajjar rivulet of J&K

2014 ◽  
Vol 15 (1&2) ◽  
pp. 95-98
Author(s):  
Indu Bhushan Sharma ◽  
Arvind Kumar Yadav ◽  
Vijeshwar Verma
Keyword(s):  
Water Samples ◽  
Microbial Contamination ◽  
Well Being ◽  
Total Hardness ◽  
Pollution Load ◽  
Chemical Parameters ◽  
Physical Chemical ◽  
Physico Chemical ◽  
Microbiological Parameters ◽  
Health And Well Being

Water quality is an index of health and well being of a society. The pollution of water bodies is one of the areas of major concern to environment. In the present investigation an attempt has been made to evaluate the physical, chemical and microbiological parameters of Jhajjar rivulet of J&K. The study has been carried out by taking water samples at three different sites of the rivulet i.e. site 1, site 2 and site3. During the study, the physico-chemical parameters like pH, temperature, colour, total hardness, BOD, COD, DO, TDS and TSS in the water samples were studied. In microbiological examination, the presence of coliform in the water samples were studied.  It has been observed that there was a pollution load mostly at site 3 and slightly at site 2, it may be due to the release of huge quantities of sewage, effluent and manmade activities at the respective sites but the water from all the sites was found free from microbial contamination. It was concluded that water at site 1 is suitable for drinking as compared to site 2 and site 3 of the rivulet.

scholarly journals Aerobic Bacteria in the Diaphragmatic Portion of Stethoscope of Medical Professionals of Tertiary Care Hospital

2015 ◽  
Vol 53 (199) ◽  
pp. 166-168 ◽  
Author(s):  
Gajendra Bham ◽  
Jeetendra Bhandari ◽  
Madan Ratna Neupane ◽  
Roji Dawadi ◽  
Prasil Pradhan
Keyword(s):  
Tertiary Care ◽  
Health Sciences ◽  
Hospital Environment ◽  
Aerobic Bacteria ◽  
Medical Professionals ◽  
Coagulase Negative Staphylococcus ◽  
Care Hospital ◽  
Gram Positive ◽  
Cross Sectional ◽  
Single Strain

Introduction: Hospital environment is a reservoir of wide varieties of microorganisms which are frequently reported colonizing in medical equipment. Stethoscopes are essential tools and of universal use in the medical profession, which might be a source of spreading nosocomial infections. This research project was conducted with an aim to assess the presence of aerobic bacteria in the stethoscope of the medical doctors working at Patan Hospital and students ofPatan Academy of Health Sciences. Methods: It is a cross sectional study based on structured questionnaire and sample assessment from the stethoscope of doctors and students of Patan Hospital and Patan Academy of Health Sciences. The stethescopes used by the doctors of five major departments of Patan Hospital and students of clinical years were included in this study. Results: Total of 99 stethoscope owned by different level of professionals (positions) and different departments were examined for bacterial contamination. Out of them, 36 were found to be considerably contaminated. Single strain of bacteria was grown from a single stethoscope. Among them 34 were Gram positive and remaining were Gram negative. Out of 34 gram postive bacteria, 29 were identified as Staphylococcus aureus, six were identified as Coagulase Negative Staphylococcus and remaining were Gram positive bacilli. Conclusions: There is presence of aerobic bacteria in diaphragmatic portion of stethoscope of medical professional of which the gram positives were the commonest.  Keywords: anaerobic bacteria; stethescope; medical professionals; patan hospital.  

scholarly journals Susceptibility patterns of bacteria isolated from the hospital environment towards disinfectants commonly used for surfaces and medical devices

2021 ◽  
Vol 319 ◽  
pp. 01081
Author(s):  
Samira Jaouhar ◽  
Abdelhakim El Ouali Lalami ◽  
Jawad Bouzid ◽  
Ikrame Zeouk ◽  
Khadija Bekhti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Medical Devices ◽  
Staphylococcus Epidermidis ◽  
Pathogenic Bacteria ◽  
Hospital Environment ◽  
Gram Positive ◽  
Microdilution Method ◽  
Bacillus Spp ◽  
Didecyldimethylammonium Chloride

This study aimed to evaluate the bactericidal activity of common disinfectants used for surfaces and medical devices. Sodium hypochlorite (D1), disinfectant (D2) composed of N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine, chloride de didecyldimethylammonium, and disinfectant (D3) composed of Didecyldimethylammonium chloride and Polyhexamethylene biguanide hydrochloride, were tested against 15 strains isolated from the hospital environment and four reference bacteria. The microdilution method was performed to assess antimicrobial activity. The susceptibility was evaluated by comparing the minimum inhibitory dilution with the dilution of disinfectant recommended by the manufacture. D1 and D2 were active against Staphylococcus epidermidis, Staphylococcus saprophyticus, Enterobacter cloacae, Escherichia coli, Pseudomonas fluorescens, Methicillin-resistant Staphylococcus aureus, Bacillus spp, Corynebacterium spp, Gram-positive bacillus, Escherichia coli ATCC 25922, Bacillus subtilis ATCC 3366, and Pseudomonas aeruginosa ATCC 27853 strains but not active against Micrococcus spp, and Staphylococcus aureus ATCC 29213. D3 was ineffective against Micrococcus spp, Bacillus Gram Positive, Staphylococcus epidermidis, and Escherichia coli ATCC 25922. Therefore, D1 and D2 can eliminate most pathogenic bacteria in hospitals, in comparison to D3. It is necessary to monitor the antibacterial activity of disinfectants against reference strains but also against those usually present on surfaces. The obtained results could have promising applications in controlling the emergence of nosocomial infections.

scholarly journals Prevalence and antibiotic susceptibility pattern of pathogens from urinary tract infections (UTI) in a private diagnostic laboratory in Bangladesh

2020 ◽  
Vol 6 (3) ◽  
pp. 564-569
Author(s):  
Md Habibur Rahman ◽  
Mushtaque Ahmed ◽  
Dayanidhi Sarkar ◽  
Md Asadur Rahman
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Antibiotic Susceptibility ◽  
Antimicrobial Agents ◽  
Urine Samples ◽  
Gram Positive ◽  
Diagnostic Laboratory ◽  
Gram Negative ◽  
Antibiotic Susceptibility Test ◽  
Tract Infections

Urinary tract infection (UTI) is one of the commonest infections encountered by clinicians and despite the widespread availability of antimicrobial agents UTI has become difficult to treat because of appearance pathogens with increasing resistance to antimicrobial agents. The aim and objectives of this study were to determine the pathogens causing UTI and to determine the antibiotic sensitivity status among these isolates in a diagnostic laboratory in Dhaka city. A laboratory based cross sectional survey was conducted in Popular Diagnostic Centre Ltd. Dhanmondi, Dhaka-1205, Bangladesh from July 2016 to December 2016. A total of 553 freshly voided midstream urine samples (10-20 ml) were collected in a wide mouth sterile container from patients and processed in microbiology laboratory to isolate pathogens and antibiotic susceptibility test using standard procedure. Among 553 urine sample, the culture positivity in urine samples was found to be 158 (28.57%) of which 39 (24.70%) were isolated from male patients and 119 (75.30%) from female patients. Escherichia coli (43.67%) were found to be the predominant pathogen followed by Staphylococcus spp. (16.45%), Enterococcus spp. (13.39%), Klebsiella spp. (13.29%), Candida spp. (5.70%), Acinetobacter spp. (4.43%), Psudomonas spp. (3.80%) and Proteus spp. (1.27%). Carbapenem group (Imipenem, Meropenem) were the most effective antibiotic with resistance between 0 and 5.1% of the gram negative isolates and Linezolid and Vancomycin was most effective in gram positive isolates. Nitrofurantoin was most effective both gram negative and gram positive isolates. This study finding showed That Escherichia. coli isolates were the predominant pathogens and showed increasing pattern to the commonly prescribed drugs in private practice that in turn leaves the clinicians with very few alternative options in drug for the treatment of UTIs. Asian J. Med. Biol. Res. September 2020, 6(3): 564-569

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