Toll like receptor 2(TLR2) in acne vulgaris patients before  and after oral isotretinion therapy

Background: Acne vulgaris is one of the top three most commonly encountered dermatological problems worldwide in both primary and secondary care. Human keratinocytes express functional TLR2 heterodimers. An increased expression of TLR2 was detected in the epidermis of inflammatory acne lesions, as observed in normal skin; the expression level increased with the degree of differentiation of the keratinocytes. TLR2 expression is upregulated in inflammatory acne lesions and induced by C. acnes. The current study conducted to assess the oral isotretinion treatment effect on the acne vulgaris patients by evaluated the Toll Like Receptor 2 as a major immune system marker in Acne vulgaris immune response. Methods: 50 patients with acne vulgaris seen in outpatient clinic of dermatology centre(Al-Baidhaa out clinic center) in Baghdad during the period 1/9/2017 to 1/8/2018. The diagnosis of patients were done by the specialist dermatologist only sever and moderate acne cases were chosen. Fifty serum samples were collected before oral isotretinion therapy, only forty-five serum samples were collected after 8 weeks of oral isotretinion therapy. All patients have received 0.5mg-1mg/kg/day of oral isotretinoin. The mean ages was 22 years(range, 16-36 years). A total of 50 patients, 36(72%) of patients were females and 14(28%) were males. Furthermore 43(86%) of cases have heredity history. Also control serum samples were collected from people without acne and they chose with the same mean ages. The serum samples were stored at -70°C for up to six months. ELISA test for quantitative determination of TLR2 human was done. Statistical analysis was done using T-test. Results: Highly significant statistical difference were noticed (p<0.001) when we compare study group after therapy with control group, also highly significant statistical difference was noticed (p<0.01) when we compare study group before therapy with control group. Conclusion: Significant decrease of TLR-2 level was noticed in study group after isotretinoin therapy in comparison with same group before therapy and also with the control group. Pronouncedly the effects of oral isotretinion suggest a path towards importance of this therapy as an anti-acne agent

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Postoperative analgesia effects of sulfentanyl plus dexmedetomidine in patients received VATS

Pteridines ◽

10.1515/pteridines-2020-0007 ◽

2020 ◽

Vol 31 (1) ◽

pp. 55-60

Author(s):

Haoyu Jiang ◽

Ying Zheng ◽

Chang Liu ◽

Ying Bao

Keyword(s):

Statistical Difference ◽

Control Group ◽

Control Groups ◽

Significant Statistical Difference ◽

Post Surgery ◽

Intravenous Analgesia ◽

The Mean ◽

Nsclc Patients ◽

And Control ◽

Time Point

AbstractBackground To evaluate sulfentanyl combined with dexmedetomidine hydrochloride on postoperative analgesia in patients who received video-assisted thoracic surgery (VATS) and its effects on serum norepinephrine (NE), dopamine (DA), 5-hydroxytryptamine (5-HT), and prostaglandin (PGE2).Material and Methods Ninety-nine non-small cell lung cancer (NSCLC) patients who received VATS were included in the study. All the patients received intravenous inhalation compound anesthesia. Of the 99 cases, 49 subjects (control group) received sulfentanyl for patient controlled intravenous analgesia (PICA) and other 50 cases (experiment group) received sulfentanyl combined with dexmedetomidine hydrochloride for PICA after operation of VATS. The analgesic effects of the two groups were evaluated according to Visual Analogue Scales (VAS) and the Bruggrmann Comfort Scale (BCS). The serum pain mediator of NE, DA, 5-HT, and PGE2 were examined and compared between the two groups in the first 24 h post-surgery.Results The VAS scores for the experiment group were significant lower than that of control group on the time points of 8, 16, and 24 h post-surgery (pall<0.05), and the BCS scores of the experiment group in the time points of 8, 16, and 24 h were significantly higher than that of controls (p<0.05). However, the VAS and BCS scores were not statistical differently in the time point of 1, 2, and 4 h post-surgery (pall>0.05). The mean sulfentanyl dosage was 63.01 ± 5.14 μg and 67.12 ± 6.91 μg for the experiment and control groups respectively with significant statistical difference (p<0.05). The mean analgesic pump pressing times were 4.30 ± 1.31 and 5.31 ± 1.46 for experiment and control groups respectively with significant statistical difference (p<0.05). The serum NE, DA, 5-HT, and PGE2 levels were significantly lower in the experimental group compared to that of control group in the time point of 12 h post-surgery (pall<0.05). The side effects of nausea, vomiting, delirium, rash, and hypotension atrial fibrillation were not statistically different between the two groups (pall>0.05).Conclusion Patient controlled intravenous analgesia of sulfentanyl combined with dexmedetomidine hydrochloride was effective in reducing the VAS score and serum pain mediators in NSCLC patients who received VAST.

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The effect of Hyoscine Butylbromide on the rate of vaginal delivery in cases of secondary arrest of cervical dilatation: A rondomized controlled clinical trial

QJM ◽

10.1093/qjmed/hcab115.006 ◽

2021 ◽

Vol 114 (Supplement_1) ◽

Author(s):

Mohamed Ahmed Elkady ◽

Dina Yahia Mansour ◽

Alyaa Mostafa Elshamy

Keyword(s):

Vaginal Delivery ◽

Statistical Difference ◽

Saline Group ◽

Cervical Dilatation ◽

Control Group ◽

Controlled Clinical Trial ◽

Hyoscine Butylbromide ◽

Labor Time ◽

Significant Statistical Difference ◽

Significant Difference

Abstract Background For decades, health providers have worked to manage labor actively and safely, with the goal of shortening the duration of painful labor. Reduction of cesarean sections and maternal complication is also an important aspect of labor management. Antispasmodic are commonly administrating during labor in both developing and developed countries, although there is a paucity of scientific reports validating this. Hyoscine butylbromide is an effective antispasmodic drug. Therefore, We aim to assess efficacy of Hyoscine butylbromide on rate of vaginal delivery in cases of 2ry arrest of cervical dilatation. Objective To assess whether the intravenous injection of hyoscine butylbromide is effective in progress of labor in which cases with 2ry arrest of cervical dilatation. Patients and Methods The study included 100 pregnant women (PG, P1, P2, P3) conducted at Ain Shams University Maternity Hospital, the inclusion Criteria were: term uncomplicated singleton pregnancy in a spontaneous active labor, with cephalic presentation. The patient must be free from any medical disorder associated with pregnancy and free from any obstetric complication. Cases were subdivided into 2 groups: Study group: 50 patients received 40mg iv Hyoscine Butylbromide (as Buscopan 20mg\mrl) when the cervix >4cm dilated and was >50% effaced. Control group: 50 patients received 2ml saline as placebo. Results Significant increase in vaginal delivery rate (72%) in Hyoscine group; compared to Saline group (52%); with significant statistical difference (p = 0.04). Highly significant decrease in 1st and 2nd stage labor time in Hyoscine group; compared to Saline group; with highly significant statistical difference (p < 0.05 respectively). These results observed without significant difference as regards 3rd stage labor time and postpartum hemorrhage (p > 0.05), APGAR score and NICU admission (p > 0.05). Conclusion From the current study we can conclude that HBB 40 mg is safe and effective in increasing vaginal delivary rate in cases of 2ry arrest of 12 dilatation. It is effective in decreasing first and second stage of labor. It has no maternal or neonatal adverse effect. It is easy and available.

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Qualitative and quantitative aspects of pain in lateral posterior thoracotomy patients

Revista Latino-Americana de Enfermagem ◽

10.1590/s0104-11692006000500011 ◽

2006 ◽

Vol 14 (5) ◽

pp. 708-712 ◽

Cited By ~ 3

Author(s):

Thaiza Teixeira Xavier ◽

Gilson de Vasconcelos Torres ◽

Vera Maria da Rocha

Keyword(s):

Pain Intensity ◽

Statistical Difference ◽

Descriptive Study ◽

Pain Scale ◽

Study Group ◽

Men And Women ◽

Evaluation Form ◽

Qualitative And Quantitative ◽

Significant Statistical Difference ◽

Lateral Posterior

Descriptive study that proposed to compare the qualitative and quantitative behavior of the pain in lateral posterior thoracotomy patients. The sample was consisted of 18 individuals with an average age of 44 years. The instruments used were physiotherapy evaluation form, numerical pain scale and McGill questionnaire for pain. The pain on the numerical pain scale was considered moderate(5) for both sexes. The descriptors of the McGill questionnaire choosen by the patients with higher frequency were: in the sensorial component, beat4, pointed1, shock2, final and pull2; in the afetive component, tired1, bored1, punishald1 and miserable1 and in the evaluative component was flat. The characteristics of pain in the sensorial group were more evidents on male group. No significant statistical difeferences were observed between quantitative answers concerning pain between the men and women. On the qualitative aspects , was observed an predominancy of the same descriptors of pain in afetive component for both sexes. Pain intensity was categorized as moderate. No significant statistical difference were observed between the pain on the post-operatory lateral posterior thoracotomy. These data demonstrate a necessity for an analysis with a larger study group.

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Examining Metabolic Profiles in Opioid-‎Dependent Patients ‎

International Journal of Medical Toxicology and Forensic Medicine ◽

10.32598/ijmtfm.v10i3.28681 ◽

2020 ◽

Vol 10 (3) ◽

pp. 28681

Author(s):

Nader Molavi ◽

Amir Ghaderi ◽

Hamid Reza Banafshe

Keyword(s):

Control Group ◽

Study Group ◽

Metabolic Profiles ◽

Opioid Use ◽

Serum Samples ◽

Social Burden ◽

Study Results ◽

Significant Difference ◽

Hs Crp ◽

Opioid Users

Background: Drug abuse is a social burden and a public health disorder. Previous evidence suggested numerous illicit substances (e.g., opioids, amphetamines, cocaine, & cannabis) affect immune system functions, oxidative stress mechanisms, inflammatory cytokines, and reactive oxygen species production. This study aimed to determine the extent of these metabolic parameters in opioid-dependent patients. We also compared these patients with a healthy control group. Methods: This study was conducted in Amirie Clinic, Kashan, Iran. Plasma and serum samples from 50 illicit opioid users (study group) and 50 non-opioid users (control group) were studied. Metabolic levels for MDA, NO, TAC, GSH, Insulin, HOMA-IR, and hs-CRP were assessed in both research groups (N=100). Results: There was a significant difference in the status of MDA (P=0.003), NO (P=0.01), TAC (P=0.003), GSH (P=0.001), insulin (P=0.04), HOMA-IR (P=0.02), and hs-CRP (P=0.001) between the study and control groups. Furthermore, there was a significant correlation among the duration of illicit opioid use and MDA concentrations (r=-0.424, P=0.002), as well as TAC levels (r=0.314, P=0.02). Conclusion: The study results suggested metabolic profiles were impaired in the study group, compared to the controls.

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Biomonitoring Data for Assessing Aflatoxins and Ochratoxin A Exposure by Italian Feedstuffs Workers

Toxins ◽

10.3390/toxins11060351 ◽

2019 ◽

Vol 11 (6) ◽

pp. 351 ◽

Cited By ~ 3

Author(s):

Barbara De Santis ◽

Francesca Debegnach ◽

Elisa Sonego ◽

Gianmarco Mazzilli ◽

Francesca Buiarelli ◽

...

Keyword(s):

Ochratoxin A ◽

Statistical Difference ◽

High Resolution Mass Spectrometry ◽

Control Group ◽

Control Groups ◽

Serum Samples ◽

Limit Of Quantification ◽

Liquid Liquid Extraction ◽

And Control

Mycotoxins exposure by inhalation and/or dermal contact is possible in different branches of industry especially where heavily dusty settings are present and the handling of dusty commodities is performed. This study aims to explore the validity of the biomonitoring as a tool to investigate the intake of mycotoxins in a population of workers operating in an Italian feed plant. Serum samples were collected for the determination of aflatoxins B1 (AFB1), AFB1-Lysine adduct and ochratoxin A (OTA). A method based on liquid–liquid extraction coupled with high resolution mass spectrometry determination was developed and fully validated. For AFB1, a high number of non-detected samples (90%) was found and no statistical difference was observed comparing workers and control group. None of the analyzed samples showed the presence of AFB1-Lysine adduct. For OTA, the 100% of the analyzed samples was positive with a 33% of the samples showing a concentration higher than the limit of quantification (LOQ), but no statistical difference was highlighted between the average levels of exposed and control groups. In conclusion, the presence of AFB1 and OTA in serum cannot be attributable to occupational exposure.

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Cryotherapy Reduced Postoperative Pain in Gynecologic Surgery: A Randomized Controlled Trial

Pain Research and Treatment ◽

10.1155/2019/2405159 ◽

2019 ◽

Vol 2019 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Apisada Chumkam ◽

Densak Pongrojpaw ◽

Athita Chanthasenanont ◽

Junya Pattaraarchachai ◽

Kornkarn Bhamarapravatana ◽

...

Keyword(s):

Postoperative Pain ◽

Statistical Difference ◽

Controlled Trial ◽

Exploratory Laparotomy ◽

Gynecologic Surgery ◽

Control Group ◽

Study Group ◽

Control Groups ◽

Vas Score ◽

And Control

Objective. To examine the effectiveness of cryotherapy for reducing postoperative pain in patients who underwent exploratory laparotomy for gynecologic surgery. Materials and Methods. Patients who had indication for an exploratory laparotomy gynecologic procedure were selected by attending physicians to undergo abdominal surgery via low transverse skin incision. The participants were randomized into study and control groups with simple random sampling methods. Cold packs were applied at two hours after operation for 6 hours. The visual analog scale (VAS) score was recorded at two, 6, and 12 hours after operation. Result. One hundred cases were recruited and then divided into study and control groups equally. The mean age of both groups was 43 years. There was no difference in demographics data of both groups. Half of the participants in both groups underwent hysterectomies. At two hours after surgery, both groups had similar VAS scores. The study group had a lower VAS score at 6 and 12 hours after surgery than the control group with statistical difference. Morphine consumption within 24 hours after surgery in both the study and control groups was 2.8±3.4 and 3.0±4.4 mg, respectively, with no statistical difference. However the registration time of the first morphine requirement in the study group was statistically more prolonged than that of the control group. The lengths of hospital stay in both groups were similar. There was no complication reported in this study. Conclusion. Cryotherapy can reduce postoperative pain. In this presented study the patients who underwent gynecologic surgery had improved pain relief and prolonged time for the first dose of the analgesic drug.

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Squamous Epithelium in the Middle Ear and Sinus

Ear Nose & Throat Journal ◽

10.1177/014556139407300112 ◽

1994 ◽

Vol 73 (1) ◽

pp. 47-48

Author(s):

Alper Tutkun ◽

Caglar Batman ◽

Cüneyt Üneri ◽

Mehmet Ali Sehitoglu

Keyword(s):

Middle Ear ◽

Statistical Difference ◽

Squamous Epithelium ◽

Normal Skin ◽

University Hospital ◽

Control Group ◽

Study Group ◽

External Ear ◽

Ear Canal ◽

Superior Part

This study has been performed between December 1990—March 1991 in the Microsurgery laboratory of the Marmara University Hospital. Twelve healthy albino guinea pigs were used as a study group while the control group consists of three animals. The potentials for cholesteatoma formation of the squamous epithelium, namely the squamous epithelium of the posterior superior part of the external ear canal skin and normal skin, were investigated. Among 24 subjects who were implanted by canal skin, cholesteatoma was fanned in 21 of them. Likewise, 19 of 24 animals implanted by normal skin came out with cholesteatoma formation. Between these two types of epithelium, there is no statistical difference in cholesteatoma formation (p >0.5).

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Toll-like Receptor 2 as a Marker Molecule of Advanced Ovarian Cancer

Biomolecules ◽

10.3390/biom11081205 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1205

Author(s):

Małgorzata Sobstyl ◽

Paulina Niedźwiedzka-Rystwej ◽

Rafał Hrynkiewicz ◽

Dominika Bębnowska ◽

Izabela Korona-Głowniak ◽

...

Keyword(s):

Ovarian Cancer ◽

Advanced Ovarian Cancer ◽

Screening Tests ◽

Control Group ◽

Newly Diagnosed ◽

Toll Like Receptor ◽

Tlr2 Expression ◽

Diagnostic Approaches ◽

Toll Like Receptor 2 ◽

Marker Molecule

Ovarian cancer is a global problem that affects women of all ages. Due to the lack of effective screening tests and the usually asymptomatic course of the disease in the early stages, the diagnosis is too late, with the result that less than half of the patients diagnosed with ovarian cancer (OC) survive more than five years after their diagnosis. In this study, we examined the expression of TLR2 in the peripheral blood of 50 previously untreated patients with newly diagnosed OC at various stages of the disease using flow cytometry. The studies aimed at demonstrating the usefulness of TLR2 as a biomarker in the advanced stage of ovarian cancer. In this study, we showed that TLR2 expression levels were significantly higher in women with more advanced OC than in women in the control group. Our research sheds light on the prognostic potential of TLR2 in developing new diagnostic approaches and thus in increasing survival in patients with confirmed ovarian cancer.

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Study on Toll-Like Receptor 2-Mediated Inflammation-Induced Familial Hypertension Combined with Hyperlipemia and Its Mechanism

Journal of Healthcare Engineering ◽

10.1155/2022/1473597 ◽

2022 ◽

Vol 2022 ◽

pp. 1-13

Author(s):

Jia Liu ◽

Chunjing Li ◽

Qiuyang Wang ◽

Haiyan Hu ◽

Chunhong Li ◽

...

Keyword(s):

Blood Pressure ◽

Blood Lipid ◽

Normal Group ◽

Inflammatory Factors ◽

Control Group ◽

Ang Ii ◽

Toll Like Receptor ◽

High Fat ◽

In The Wild ◽

Toll Like Receptor 2

According to the latest clinical data, cardiovascular diseases have ranked first in prone diseases, causing 40% of the premature deaths of China’s population. This study aimed to investigate the influence of Toll-like receptor 2- (TLR2-) mediated inflammation on the occurrence and development of familial hypertension combined with hyperlipemia and its related mechanism. Blood specimens from 66 patients undergoing coronary atherosclerosis were collected and grouped, including 22 patients into the control group, 25 into the familial hypertension group, and 19 into familial hypertension combined with hyperlipemia group. In this study, ELISA was conducted for determining the levels of four inflammatory factors of TLR2 and IL-1β, IL-6, TNF-ɑ, and CCL2 in serum and the levels of relevant indicators in mice. C57Bl/6j and genetically engineered C.129(B6)-Tlr2tm1Kir/J mice were given subcutaneous injection of normal saline (wild-saline group), 8-week 40% high-fat diet (wild-high-fat group), and subcutaneous Alzet-implanted angiotensin II micropump supplemented with the research diet (wild-high fat-Ang II group, Tlr2-/--high fat-Ang II group). Blood pressure in mice was recorded consecutively with a noninvasive hemopiezometer for eight weeks. TLR2 and IL-1β, IL-6, TNF-ɑ, and CCL2 in serum of patients with familial hypertension combined with hyperlipemia and the hypertension combined with hyperlipemia mouse model were higher than those in the normal group. Under combined intervention of Ang II and the research diet, mRNA expression related to blood pressure, blood lipid, and fat metabolism in Tlr2-/- genetically engineering mice was significantly lower than that in the wild-high fat-Ang II group. The phosphorylation levels of AKT, IKK, and p65 in mice with hypertension combined with hyperlipidemia were significantly higher than those in normal group. The levels of blood pressure and blood lipid in mice after blocking the AKT or NF-κB pathway were significantly downregulated compared with those in the wild-high fat-Ang II group, with statistically significant differences (both P < 0.05 ). In conclusion, TLR2 regulates inflammation through Akt-NF-κB pathway, thus inducing the occurrence and development of familial hypertension combined with hyperlipemia.

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Toll-Like Receptor 2 (TLR2) and TLR4 Mediate the IgA Immune Response Induced by Mycoplasma hyopneumoniae

Infection and Immunity ◽

10.1128/iai.00697-19 ◽

2019 ◽

Vol 88 (1) ◽

Cited By ~ 3

Author(s):

Xia Li ◽

Yun-ke Zhang ◽

Bao Yin ◽

Jing-bo Liang ◽

Fei Jiang ◽

...

Keyword(s):

Lymph Nodes ◽

B Cell ◽

Mucosal Immunity ◽

Mycoplasma Hyopneumoniae ◽

Mouse Lung ◽

Control Group ◽

Toll Like Receptor ◽

Content Type ◽

Coculture Model ◽

Toll Like Receptor 2

ABSTRACT IgA plays an important role in mucosal immunity against infectious pathogens; however, the molecular mechanism of IgA secretion in response to infection remains largely unknown, particularly in Mycoplasma spp. In this study, we found that the levels of IgA in the peripheral blood serum, bronchoalveolar lavage fluid, nasal mucosa, trachea, hilar lymph nodes, and lung tissues of pigs increased significantly after infection with Mycoplasma hyopneumoniae. Furthermore, IgA and CD11c were detected in the lungs and hilar lymph nodes by immunohistochemical analysis, and colocalization of these two markers indicates that CD11c+ cells play an important role in IgA mucosal immunity induced by M. hyopneumoniae. To investigate the regulatory mechanism of IgA, we separated mouse dendritic cells (DCs) from different tissues and mouse macrophages from the lungs and then cultured mouse B cells together with either DCs or macrophages in vitro. In the mouse lung-DC/B (LDC/B) cell coculture, IgA secretion was increased significantly after the addition of whole-cell lysates of M. hyopneumoniae. The expression of both Toll-like receptor 2 (TLR2) and TLR4 was also upregulated, as determined by mRNA and protein expression analyses, whereas no obvious change in the expression of TLR3 and TLR7 was detected. Moreover, the IgA level decreased to the same as the control group when TLR2 or TLR4 was inhibited instead of TLR8 or TLR7/9. In conclusion, M. hyopneumoniae can stimulate the response of IgA through TLR2 and TLR4 in a mouse LDC/B cell coculture model, and the coculture model is an ideal tool for studying the IgA response mechanism, particularly that with Mycoplasma spp.

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