scholarly journals Effect of Application of Remineralizing Agents on the Microhardness of Microabraded Teeth

2015 ◽  
Vol 6 (3) ◽  
pp. 174-177
Author(s):  
S Srilatha ◽  
Zeeshan Hasanali Ladhani ◽  
Sayli Dargad ◽  
Vighnesh Dixit
Keyword(s):  
Calcium Phosphate ◽  
Topical Application ◽  
Artificial Saliva ◽  
Control Group ◽  
List Type ◽  
Anterior Teeth ◽  
Casein Phosphopeptide ◽  
Group B ◽  
Vicker’S Microhardness ◽  
Group D

ABSTRACT Background To evaluate microhardness of enamel surface after microabrasion of enamel using casein phosphopeptideamorphous calcium phosphate (CPP-ACP) and casein phosphopeptide-amorphous calcium phosphate with fluoride (CPP-ACPF). Materials and methods Ten freshly extracted anterior teeth were selected. Teeth were treated with abrasive slurry. This prepared slurry was applied to the labial aspects of teeth, using a rotating rubber cup, for 50 seconds. Each tooth was divided into four parts and treated accordingly with the topical application of the demineralizing agent stored in artificial saliva. The teeth were divided into four groups, as follows: – Group A: Control group – Group B: Microabrasion done but not treated – Group C: Microabrasion done and CPP-ACP paste applied – Group D: Microabrasion done and CPP-ACPF (i.e. with fluoride) applied. The microhardness was evaluated using the Vicker's microhardness test. Results Microhardness values were highest for the control group followed by group D, group C and finally group B. Microabraded group of teeth with no topical application showed least microhardness values of all. Conclusion The CPP-ACPF increased the microabrasion significantly as compared to CPP-ACP and microabrasion group. How to cite this article Ladhani ZH, Dargad S, Dixit V, Srilatha S, Hegde V. Effect of Application of Remineralizing Agents on the Microhardness of Microabraded Teeth. World J Dent 2015;6(3):174-177.

scholarly journals Enamel Hardness Differences After Topical Application Of Theobromine Gel And Casein Phosphopeptide-Amorphous Calcium Phosphate

2020 ◽  
Vol 10 (1) ◽  
pp. 5
Author(s):  
Tamara Yuanita ◽  
Nanik Zubaidah ◽  
Mifta Izha A R
Keyword(s):  
Citric Acid ◽  
Calcium Phosphate ◽  
Topical Application ◽  
Amorphous Calcium Phosphate ◽  
Acrylic Resin ◽  
Control Group ◽  
Tooth Erosion ◽  
Hardness Tester ◽  
Group I ◽  
Casein Phosphopeptide

Background: Most soft drinks contain citric acid / phosphoric acid with pH <3.5. Strong acids in the oral cavity cause tooth erosion. Tooth erosion leads to demineralization enamel. Remineralization can prevent demineralization process. One of the commercial remineralization agents is Casein Phosphopeptide-amorphous Calcium Phosphate(CPP-ACP) but it has low solubility and less affordable price. Therefore, a natural ingredients such as theobromine from cocoa extract need to be developed as remineralization agent. Objective: To determine enamel hardness differences due to topical application of theobromine gel and CPP-ACP. Method: incisive crowns were cut off (1x1) cm then planted in cylindrical acrylic resin. All samples were immersed with citric acid for 90 minutes then divided into 3 groups. Group I (control) was immersed with aquades (96 min), group II was applied with theobromin gel 200 mg/l (96 min), group III was applied with CPP-ACP (96 min). By using vickers hardness tester, enamel hardness be measured (15 sec) at 3 points. Results: There were significant differences between the control group, CPP-ACP group, and theobromine gel group. Conclusion: Enamel hardnesse with theobromine gel application is higher than CPP-ACP application..

scholarly journals Effect of Casein Phosphopeptide-Amorphous Calcium Phosphate and Three Calcium Phosphate on Enamel Microhardness

2017 ◽  
Vol 18 (7) ◽  
pp. 583-586 ◽  
Author(s):  
En HR Haghgou ◽  
Roza Haghgoo ◽  
Mohamad R Roholahi ◽  
Zahra Ghorbani
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Artificial Saliva ◽  
Testing Machine ◽  
Third Molar ◽  
Negative Control ◽  
Control Group ◽  
Healthy Human ◽  
Significant Difference ◽  
Casein Phosphopeptide

ABSTRACT Aim This study aims to investigate the effect of casein phosphopeptide-amorphous calcium phosphate and three calcium phosphate (CPP-ACP and TCP) on increasing the microhardness of human enamel after induction of erosion. Materials and methods A total of 26 healthy human-impacted third molar teeth were chosen, and their hardness measured using a microhardness testing machine. The samples were immersed in Coca Cola (pH = 4.7) for 8 minutes. Then, microhardness was measured again, and these samples were randomly divided into four groups (two control groups and two experimental groups). (1) Negative control group: Artificial saliva was used for 10 minutes, (2) positive control group: Fluoride gel was used for 10 minutes, (3) β-TCP group: TCP was used for 10 minutes, (4) CCP-ACP group: CCP-ACP was used for 10 minutes. The final microhardness of those samples was measured, and the changes in microhardness of teeth within group and between groups were analyzed using the paired and analysis of variance tests respectively. Results were considered statistically significant at a level of p < 0.05. Results No significant difference was observed in microhardness between CPP-ACP group and TCP group (p = 0.368) during the time microhardness significantly dropped after soaking in soda. Conclusion Casein phosphopeptide-amorphous calcium phosphate and TCP increased the microhardness of teeth. The increase in hardness in the TCP group was higher than in the CPP-ACP group, but this difference was not significant (p = 0.36). Clinical significance Casein phosphopeptide-amorphous calcium phosphate and TCP can affect the remineralization of erosive lesions. How to cite this article Haghgou EHR, Haghgoo R, Roholahi MR, Ghorbani Z. Effect of Casein Phosphopeptide-Amorphous Calcium Phosphate and Three Calcium Phosphate on Enamel Microhardness. J Contemp Dent Pract 2017;18(7):583-586.

Comparison of CPP-ACP, Tri-Calcium Phosphate and Hydroxyapatite on Remineralization of Artificial Caries Like Lesions on Primary Enamel -An in vitro Study

2016 ◽  
Vol 40 (5) ◽  
pp. 404-409 ◽  
Author(s):  
Meghna Bajaj ◽  
P Poornima ◽  
S Praveen ◽  
NB Nagaveni ◽  
KB Roopa ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Polarized Light ◽  
In Vitro Study ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Before And After ◽  
Group B ◽  
Group D

Objectives: To compare CPP-ACP, Tri-calcium phosphate and Hydroxyapatite on remineralization of artificial caries like lesions on primary enamel. Study design: Ten extracted Primary molars coated with nail varnish, leaving a window of 2×4 mm on buccal and lingual surface were immersed in demineralizing solution for 96 hours and sectioned longitudinally to obtain 40 sections (4 sections per tooth) and were randomly divided into 4 groups (A to D) n=10; Group A: negative control, Group B: CPP-ACP, Group C: Tri-calcium phosphate, Group D: Hydroxyapatite. Sections were subjected to pH cycling for 10 days and were evaluated by polarized light microscope before and after treatment. Results: Intra group comparison of demineralization and remineralization was done by paired t-test. One way ANOVA was used for multiple group comparisons followed by post HOC TUKEY'S Test for group wise comparisons. Remineralization was found more with Group D followed by Group B, C and A. Conclusion: Hydroxyapatite showed better remineralization when compared to CPP-ACP and Tri-calcium phosphate.

scholarly journals In vitro comparison of impact of different bleaching Agents on the microhardness of Enamel

2016 ◽  
Vol 17 (3) ◽  
pp. 258-262 ◽  
Author(s):  
Sudarshan C Pujari ◽  
Subhra Dey ◽  
Vinisha Pandey ◽  
Neha Awasthi
Keyword(s):  
Artificial Saliva ◽  
Study Groups ◽  
Knoop Hardness ◽  
Minimal Invasive ◽  
Control Group ◽  
Tooth Structure ◽  
Group A ◽  
Group B ◽  
Group D

ABSTRACT Background Various agents are used these days for increasing the esthetics. One such procedure is bleaching that offers various advantages, as it is minimal invasive and cheap option to color the teeth and remove stain. The altered enamel after the bleaching process shows surface demineralization and porosities. The present study aimed to evaluate the effect of different bleaching agents on the microhardness of enamel. Materials and methods A total of 100 freshly human extracted maxillary premolar teeth were selected for the study. Teeth with sound tooth structure were included for the study. All the specimens were randomly divided into four groups with 25 specimens in each group depending upon the type of bleaching agent used: Group A, artificial saliva (Control group); Group B, 35% hydrogen peroxide (HP); Group C, 25% HP; Group D, 10% carbamide peroxide (CP). Knoop Hardness Number (KHN) was calculated at 24, 48-hour, and 7-week interval. Results Results showed no statistical significant differences between the microhardness of enamel of different groups (p < 0.005). A slight fall in the value of KHN was seen in all the groups, except for the control group, although the results were statistically nonsignificant (p > 0.005). Conclusion Although nonsignificantly, all the bleaching solutions produced some amount of alterations in the microstructure of enamel. More studies with higher study groups and more advanced estimation technologies are required to minimize microstructure alterations and promote for better outcome of bleaching procedures. How to cite this article Dey S, Pandey V, Kumar A, Awasthi N, Sahu A, Pujari SC. In vitro comparison of impact of different bleaching Agents on the microhardness of Enamel. J Contemp Dent Pract 2016;17(3):258-262.

scholarly journals Efficacy of Nano Crystallised Calcium Phosphate (NCCP) and Casein Phosphopeptide – Amorphous Calcium Phosphate (CPP-ACP) for Controlling Postoperative Sensitivity After Power Bleaching-A Comparative In-Vivo Study

2022 ◽  
Vol 6 (1) ◽  
Author(s):  
Ramesh T ◽  
A. Shilpa ◽  
Sarjeev Singh Yadav ◽  
Kavitha. A ◽  
P. Prathibha ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Dental Fluorosis ◽  
Tooth Bleaching ◽  
Control Group ◽  
Anterior Teeth ◽  
Dental Procedures ◽  
Desensitizing Agents ◽  
Casein Phosphopeptide

Tooth bleaching is one of the most popular cosmetic dental procedures opted by the patients who desire pleasing smile. It is the simplest, least invasive means available to lighten discolored vital teeth. A number of desensitizing agents have been tried in an attempt to counteract bleaching-related sensitivity. This study was done to compare the effect of two different desensitizing agents for controlling post operative sensitivity after power bleach procedure. Sixty volunteers with mild to moderate dental fluorosis in maxillary anterior teeth, who fulfilled the inclusion and exclusion criteria, were randomly selected for this study. After the bleaching procedurea demo was given to the patient while application of desensitising gel and was instructed to use the gel for 14 days.All the patients were recalled at an interval of 1st day, 3rdday, 5th day, 7th day, and on 14th day to record the post operative sensitivity after power bleach procedure. The observations were analyzed using one way analysis of variance (ANOVA), Tukeys post hoc test. On the basis of VAS results, all the groups showed post operative sensitivity of varied intensity at different intervals. NCCP showed less sensitivity followed by CPP-ACP and Control group. Keywords: Power Bleaching, Post operative sensitivity, Nano Crystallized Calcium Phosphate, Casein Phosphopeptide-Amorphous Calcium Phosphate

Hepatoprotective Role of Propolis Extract to Prevent Hepatotoxic Effects of ATT in patients with Tuberculosis

2021 ◽  
Vol 15 (9) ◽  
pp. 2867-2869
Author(s):  
Asma Arshad ◽  
Saira Munawar ◽  
Rabia Sajjad Toor ◽  
Saba Saleem ◽  
Kanwal Sharif ◽  
...  
Keyword(s):  
Standard Dose ◽  
Ethanolic Extract ◽  
Control Group ◽  
Albino Rats ◽  
P Value ◽  
Average Weight ◽  
Propolis Extract ◽  
Measured Weight ◽  
Group B ◽  
Group D

Objective: The main purpose of this study is to evaluate the hepatoprotective effects of propolis in hepatocytes injury caused by ATT due to isoniazid and rifampicin. Methods: Healthy albino rats of with average weight of 200-250g were under this study. These rats dividing into main four groups, A group is taken a control group and then further into the group B, group C, and group D as group for experiments. The control group had 15 rats with measured weight, they were given distilled water. Group B had 15 rats, they were given with standard dose of rifampicin and isoniazid. Group c had 15 rats, they were also given with standard dose of rifampicin and isoniazid. Group D had 15 rats, they were given with standard dose of rifampicin and isoniazid and also extract of the propolis we prepared. Results: Serum ALT in the experimental group B with group C, group D were also found to be of statistically significant with p-value < 0.001. ALT serum level observed high in group B. Multiple comparison between groups revealed that group B with a significantly increase in the serum enzyme AST level in comparison to group A, group C and group D with p-value <0.001. Conclusion: This study showed that ethanolic extract of propolis prevents isoniazid and rifampicin induced hepatotoxicity in the albino rats. Key words; Propolis, Anti-tuberculosis treatment, Hepato-toxicity.

Silymarin and dimercaptosuccinic acid ameliorate lead-induced nephrotoxicity and genotoxicity in rats

2015 ◽  
Vol 35 (4) ◽  
pp. 398-403 ◽  
Author(s):  
Y Alcaraz-Contreras ◽  
RP Mendoza-Lozano ◽  
ER Martínez-Alcaraz ◽  
M Martínez-Alfaro ◽  
MA Gallegos-Corona ◽  
...  
Keyword(s):  
Lead Acetate ◽  
Chelating Agent ◽  
Blood Lead ◽  
Positive Control ◽  
Dimercaptosuccinic Acid ◽  
Control Group ◽  
Lead Levels ◽  
Group A ◽  
Group B ◽  
Group D

We studied the effect of silymarin and dimercaptosuccinic acid (DMSA), a chelating agent that was administered individually or in combination against lead (Pb) toxicity in rats. Wistar rats (200 ± 20) were randomly divided into five groups. Group A served as a control. Groups B–E were exposed to 2000 ppm of lead acetate in drinking water for 8 weeks. Group B served as a positive control. Group C received silymarin (100 mg kg−1 orally) for 8 weeks. Group D received DMSA (75 mg kg−1 orally) once daily for the last 5 days of treatment. Group E received DMSA and silymarin as groups C and D, respectively. The effect of Pb was evaluated and accordingly the treatments on blood lead levels (BLLs), renal system, and genotoxic effects were calculated using comet assay. The BLLs were significantly increased following the exposition of lead acetate. The administration of silymarin and DMSA provided reduction in BLLs. Silymarin and DMSA provided significant protection on the genotoxic effect of Pb. The toxic effect of Pb on kidneys was also studied. Our data suggest that silymarin and DMSA improve the renal histopathological lesions.

scholarly journals Effects of aqueous leaf extract of Corchorus olitorius on the testis and blood testosterone level of adult Wistar rats

2021 ◽  
Vol 10 (2) ◽  
pp. 024-030
Author(s):  
Godwin Chinedu Uloneme ◽  
Demian Nnabuihe Ezejindu ◽  
Darlington Cyprian Akukwu ◽  
Amadi Chibundu Chiekezie
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Day Treatment ◽  
Control Group ◽  
Corchorus Olitorius ◽  
Group A ◽  
Normal Rat ◽  
Group B ◽  
Calcium Iron ◽  
Group D

Background: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).ound: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).

scholarly journals Protective effect of LIF-huMSCs on the retina of diabetic model rats

2021 ◽  
Vol 14 (10) ◽  
pp. 1508-1517
Author(s):  
Shan-Na Chen ◽  
◽  
Ying-Xue Ma ◽  
Song Chen ◽  
Guang-Hui He ◽  
...  
Keyword(s):  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Model ◽  
Group A ◽  
Retinal Structure ◽  
Hs Crp ◽  
Group B ◽  
And Function ◽  
Group D ◽  
Control Group Group

AIM: To investigate the protective effect of human umbilical cord mesenchymal stem cells (hUCMSCs) modified by the LIF gene on the retinal function of diabetic model rats and preliminarily explore the possible mechanism. METHODS: A stably transfected cell line of hUCMSCs overexpressing leukemia inhibitory factor (LIF) was constructed. Overexpression was verified by fluorescent quantitative polymerase chain reaction (qPCR). Forty-eight adult Sprague-Dawley rats were randomly divided into a normal control group (group A), streptozotocin-induced diabetic control group (group B), diabetic rats at 3mo injected with empty vector-transfected hUCMSCs (group C) or injected with LIF-hUCMSCs (group D). Four weeks after the intravitreal injection, analyses in all groups included retinal function using flash electroretinogram (F-ERG), retinal blood vessel examination of retinal flat mounts perfused with fluorescein isothiocyanate-dextran (FITC-dextran), and retinal structure examination of sections using hematoxylin and eosin staining. Expression levels of adiponectin (APN), high-sensitivity C-reactive protein (hs-CRP), and neurotrophin-4 (NT-4) in each group was detected using immunohistochemistry, PCR, Western blotting, and ELISA, respectively. RESULTS: A stable transgenic cell line of LIF-hUCMSCs was constructed. F-ERG and FITC-dextran examinations revealed no abnormalities of retinal structure and function in group A, severe damage of the retinal blood vessels and function in group B, and improved retinal structure and function in group C and especially group D. qPCR, ELISA, and Western blot analyses revealed progressively higher APN and NT-4 expression levels in groups B, C, and D than in group A. hs-CRP expression was significantly higher in group B than in groups A, C, and D, and was significantly higher in group C than in group D (P<0.05). CONCLUSION: LIF-hUCMSCs protect the retina of diabetic rats by upregulating APN and NT-4 expression and downregulating hs-CRP expression in the retina.

scholarly journals Pengaruh cabe rawit terhadap gambaran histopatologik lambung tikus Wistar yang diinduksi aspirin

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Abel T. Bawulele ◽  
Lily Loho ◽  
Poppy Lintong
Keyword(s):  
Gastric Mucosa ◽  
Wistar Rats ◽  
Inflammatory Cells ◽  
Negative Control ◽  
Control Group ◽  
Histopathological Changes ◽  
Acute Gastritis ◽  
Group B ◽  
Group D ◽  
Mucosa Epithelium

Abstract: Cayenne pepper (Capsicum frustescens L ) is commonly in daily food. This study aimed to determine the effect of cayenne pepper to histopathological changes in gaster of Wistar rats induced by aspirin. This was an experimental study. Subjects were Wistar rats consisted of 5 rats as the negative control and 20 rats as the treatment group. Group A, the negative control group, was untreated; group B was treated with aspirin 21mg/day for 10 days; group C was treated with aspirin 21mg/day for 10 days together with cayenne pepper 84mg once daily; group D was treated with aspirin 21mg/day for 10 days followed by cayenne 84mg twice daily; and group E was treated with aspirin 21mg/day for 10 days and then was untreated for 3 days. The histopathological changes in group C showed a lot of inflammatory cells and erosion of gastric mucosa epithelium compared to group B, D, and E. Group D also showed inflammatory cells but no erosion as in group C. Group E had less inflammatory cells than group B, C, and D. Conclusion: Administration of aspirin 21mg/kg BW for 10 days led to acute gastritis in Wistar rat. Administration of aspirin together with cayenne 84mg/day led to acute gastritis and erosion of gastric mucosa epithelium. However, administration of cayenne pepper 168mg/day after aspirin led to acute gastritis without erosionKeywords: pepper, gaster, aspirinAbstrak : Cabe Rawit (Capsicum frustescens L.) banyak dijumpai dalam makanan sehari-hari. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian cabe rawit terhadap gambaran histopatologik lambung tikus wistar yang diinduksi aspirin. Jenis penelitian ialah eksprimental. Subjek penelitian ialah tikus wistar, yang dibagi atas: 5 ekor untuk kontrol negatif dan 20 ekor untuk perlakuan. Kelompok A (kontrol negatif) tidak diberi perlakuan; kelompok B diberikan aspirin 21mg/hari selama 10 hari; kelompok C diberikan aspirin 21mg/hari selama 10 hari bersama cabe rawit 84mg sekali sehari; kelompok D diberikan aspirin 21mg/hari selama 10 hari kemudian dilanjutkan pemberian cabe rawit 84mg selama 3 hari 2 kali sehari; dan kelompok E diberikan aspirin 21mg/hari selama 10 hari kemudian tidak diberi perlakuan selama 3 hari. Hasil penelitian menunjukkan bahwa pada kelompok C terlihat banyak sel-sel radang disertai erosi epitel mukosa lambung dibanding dengan kelompok B, D, dan E. Pada kelompok D pemberian aspirin dilanjutkan dengan cabe rawit dosis 2 kali sehari masih terdapat sel-sel radang tetapi tidak terjadi erosi seperti pada kelompok C. Kelompok E memiliki sel-sel radang yang lebih sedikit dibandingkan kelompok B, C, dan D. Simpulan: Pemberian aspirin 21mg/hari selama 10 hari menyebabkan gastritis akut pada tikus wistar. Pemberian aspirin bersama cabe rawit 84mg/hari menyebabkan gastritis akut erosi sedangkan pemberian cabe rawit 168mg/hari setelah diberi aspirin menyebabkan gastritis akut tanpa erosi.Kata kunci: cabe rawit, lambung, aspirin

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