scholarly journals Comparative Evaluation of Myeloperoxidase Enzymatic Activity in Gingival Crevicular Fluid of Subjects having Orthodontic Treatment by Different Aligning Arch Wires

2017 ◽  
Vol 18 (10) ◽  
pp. 977-980 ◽  
Author(s):  
Vinny Bhasin ◽  
Madhavi Singh ◽  
Manish Goutam ◽  
Shuchi Singh ◽  
Adamya S Nigam ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Comparative Evaluation ◽  
Orthodontic Treatment ◽  
Gingival Crevicular Fluid ◽  
Metabolic Response ◽  
Study Groups ◽  
Time Intervals ◽  
Anterior Teeth ◽  
The Mean ◽  
Crevicular Fluid

ABSTRACT Introduction There exist a number of factors that affect the outcome of orthodontic treatment. These factors can be assessed by various gingival markers. One such maker is myeloperoxidase (MPO). Hence, we planned the present study to assess and compare the MPO activity in the gingival crevicular fluid (GCF) of subjects undergoing orthodontic treatment by different aligning arch wires. Materials and methods The present study included assessment of patients who underwent orthodontic treatment for crowding of anterior teeth. Diagnostic cast models of all the subjects were made for recording the irregularity index. All the subjects were randomly divided into three study groups with 15 patients in each group based on the type of nickel–titanium (NiTi) arch wires used. A collection of GCF samples was done in all the patients at various time intervals and it was sent to the laboratory for assessment of MPO activity. Activity of the MPO enzyme was expressed in terms of number of units per 100 µL. All the results obtained were compiled and analyzed by Statistical Package for the Social Sciences (SPSS) software. Results We observed that nonsignificant results were obtained while comparing the mean age and mean gingival score in all the study groups. However, significant results were obtained on comparing the mean MPO enzymatic activity in all the study groups at different time intervals. Conclusion Both superelastic NiTi and heat-activated NiTi generate optimal forces, which are necessary for higher metabolic response of the periodontal ligament. Clinical significance In the intimal stages of orthodontic treatment, both superelastic NiTi and heat-activated NiTi wires are superior in leveling and aligning the crowded teeth. How to cite this article Bhasin V, Singh M, Goutam M, Singh S, Nigam AS, Joshi A. Comparative Evaluation of Myeloperoxidase Enzymatic Activity in Gingival Crevicular Fluid of Subjects having Orthodontic Treatment by Different Aligning Arch Wires. J Contemp Dent Pract 2017;18(10):977-980.

scholarly journals Gingival Crevicular Fluid Turnover Markers in Premenopausal vs Postmenopausal Women receiving Orthodontic Treatment

2017 ◽  
Vol 18 (10) ◽  
pp. 933-936
Author(s):  
Anusha Bitra ◽  
B Jhansi Rani ◽  
Anuj S Parihar ◽  
Gopinath P Vynath ◽  
Shekhar Grover
Keyword(s):  
Postmenopausal Women ◽  
Orthodontic Treatment ◽  
Gingival Crevicular Fluid ◽  
Study Groups ◽  
Bone Biomarkers ◽  
Mean Values ◽  
The Mean ◽  
Turnover Markers ◽  
Crevicular Fluid ◽  
Orthodontic Forces

ABSTRACT Background Orthodontic treatment is one of the commonly used dental treatments. Orthodontic forces act on the bone by modulating the biomolecules, chiefly the osteoprotegerin (OPG), osteopontin (OPN), receptor activator of nuclear factor kappa-B (RANK), and RANK ligand (RANKL) (OPG ligand). Hormonal changes are known to cause marked alteration in the levels of these biomolecules. Hence, we planned this study to evaluate the response of bone biomarkers in the gingival crevicular fluid (GCF) in postmenopausal women undergoing fixed orthodontic therapy. Materials and methods This study included assessment of 50 subjects who underwent orthodontic treatment from June 2012 to July 2016. All the patients were divided into two study groups with 25 patients in each group: premenopausal group and postmenopausal group. Similar orthodontic wires were used for controlling the forces applied in subjects of both the study groups and their GCF levels of RANKL, and OPN was assessed at baseline and 24 hours after the activation of orthodontic forces. All the results were compiled, assessed, and analyzed by Statistical Package for the Social Sciences software version 16.0. Chi-square test, Student's t-test, and Mann–Whitney U test were used for the assessment of the level of significance. Results The mean values of RANKL and OPN in the premenopausal and postmenopausal groups were found to be 241.52 and 317.15 pg/µL respectively. The mean values of RANKL at baseline in the premenopausal and postmenopausal groups were found to be 7.15 and 3.84 pg/µL respectively. Nonsignificant results were obtained while comparing mean OPN and RANKL level alteration in between the two study groups. Conclusion The mean alterations in the GCF levels of bone biomarkers are similar for both premenopausal and postmenopausal women. Clinical significance For women with either premenopausal or postmenopausal status, orthodontic treatment appears to be equally safer. How to cite this article Bitra A, Rani BJ, Agarkar SS, Parihar AS, Vynath GP, Grover S. Gingival Crevicular Fluid Turnover Markers in Premenopausal vs Postmenopausal Women receiving Orthodontic Treatment. J Contemp Dent Pract 2017;18(10):933-936.

scholarly journals Assessment of Changes in Nickel and Chromium Levels in the Gingival Crevicular Fluid during Fixed Orthodontic Treatment

2017 ◽  
Vol 18 (8) ◽  
pp. 675-678
Author(s):  
Vinny Bhasin ◽  
Swati J Pustake ◽  
Viprat Joshi ◽  
Anil Tiwari ◽  
Meenakshi Bhasin ◽  
...  
Keyword(s):  
Orthodontic Treatment ◽  
Gingival Crevicular Fluid ◽  
High Strength ◽  
Atomic Absorption Spectrophotometry ◽  
Induced Response ◽  
Mean Values ◽  
Ph Variation ◽  
Gingival Health ◽  
The Mean ◽  
Crevicular Fluid

ABSTRACT Background Various components of appliances used in fixed orthodontic treatment are fabricated from materials that are highly resistant in nature and have high strength and biocompatibility. Corrosion of materials occurs inside the oral cavity due to numerous environmental or oral factors that act on them. These factors include temperature, pH variation, salivary conditions, mechanical loads, microbiological and enzymatic activity, and various food components. Gingival crevicular fluid (GCF) is the material obtained from the gingival sulcus and might act as a potential source for various biomarkers in the orthodontic setup because inflammatory-induced response is directly related to orthodontic forces in GCF. In the light of above-mentioned data, we planned this study to assess and evaluate the changes occurring in nickel and chromium levels in the GCF during fixed orthodontic treatment. Materials and methods This study included assessment of 30 patients who underwent fixed orthodontic treatment. Three samples were taken from the GCF of the patients giving a total of 90 samples. The samples were collected at the following time intervals: At baseline (pretreatment time), 1 month after the start of orthodontic treatment, and at 6 months after the commencement of orthodontic treatment. Cellulose strips were used for isolation of the tooth region. For GCF collection, a standardized cellulose acetate absorbent strip was used. Placement of the strips was done in the sulcus for 60 seconds for the collection of the samples. Refrigeration of the specimen bottles was done for a minimum of 7 days and was then sent to a laboratory where specimens were transferred for atomic absorption spectrophotometry. All the results were analyzed by Statistical Package for the Social Sciences software. Results At 1 month, the mean value of nickel and chromium in GCF was found to be 4.5 and 4.9 μg/gm of GCF respectively. While comparing the mean nickel levels between 1 and 6 months and between baseline and 6 months, significant results were obtained. Significant results were also obtained while comparing the mean values of chromium in GCF between baseline and 6 months and between 1 and 6 months. Gingival health index of the patients was found to be associated with increased inflammation with the progression of time of orthodontic treatment. Conclusion Levels of nickel and chromium might show considerable elevation in the GCF with time along with an increase in the severity of inflammation in the gingival health in patients undergoing fixed orthodontic treatment. Clinical significance Regular oral prophylaxis of the patients undergoing orthodontic treatment should be done to avoid toxicities caused by the release of nickel and chromium and for maintenance of good oral hygiene and oral health. How to cite this article Bhasin V, Pustake SJ, Joshi V, Tiwari A, Bhasin M, Punia RS. Assessment of Changes in Nickel and Chromium Levels in the Gingival Crevicular Fluid during Fixed Orthodontic Treatment. J Contemp Dent Pract 2017;18(8):675-678.

scholarly journals Pentraxin-3 Levels in Gingival Crevicular Fluid during Canine Retraction with Nickel–Titanium Coil Spring and Active Tieback

2017 ◽  
Vol 18 (8) ◽  
pp. 710-713
Author(s):  
Sukhpreet Mangat ◽  
Rakesh Thukral ◽  
Anvesha Ganguly ◽  
Harleen Bali ◽  
Shekhar Grover
Keyword(s):  
Orthodontic Treatment ◽  
Gingival Crevicular Fluid ◽  
Nickel Titanium ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pentraxin 3 ◽  
Coil Spring ◽  
Time Intervals ◽  
Canine Retraction ◽  
Significant Difference ◽  
Crevicular Fluid

ABSTRACT Aim Orthodontic treatment is routinely carried out in patients with the purpose of correcting various forms of dental malocclusions. Retraction of the canines can be achieved either individually or along with incisor. Pentraxin-3 (PTX-3) is regarded as the true independent indicator of disease activity. Hence, we undertook the present study to assess and compare the level of PTX-3 in patients undergoing canine retraction with active tieback and Nickel–Titanium (NiTi) coil spring. Materials and methods The present study included assessment of 25 patients that underwent canine retraction as a part of fixed orthodontic treatment. In the maxillary right and left quadrant, active tieback and NiTi coil spring were used respectively. Gingival crevicular fluid samples were collected 1 hour prior to the commencement of orthodontic canine retraction procedure followed by collection at following time intervals: 1 hour, 1, 7, and 14 days after the start of canine retraction procedure. Enzyme-linked immunosorbent assay kit was used for analysis of the samples as per manufacturer's instructions. All the data were recorded and compiled. All the results were analyzed by Statistical Package for the Social Sciences software. Results Nonsignificant difference was observed in the values of PTX-3 at baseline and 1 hour. While comparing the mean PTX-3 values between different time intervals in both the study groups, significant difference was observed. Conclusion In comparison to the active tieback, NiTi coil exhibited faster space closure rate. Clinical significance In patients undergoing orthodontic treatment, PTX-3 is associated with periodontal remodeling under the effect of orthodontic forces. How to cite this article Thukral R, Mangat S, Ganguly A, Agarkar SS, Bali H, Grover S. Pentraxin-3 Levels in Gingival Crevicular Fluid during Canine Retraction with Nickel–Titanium Coil Spring and Active Tieback. J Contemp Dent Pract 2017; 18(8):710-713.

scholarly journals Assessment of Myeloperoxidase Levels In Relation to the Nature and Duration of Orthodontic Forces – An In vivo, Split Mouth Study

2018 ◽  
Vol 11 (4) ◽  
pp. 2061-2067
Author(s):  
Vineet Sharma ◽  
Supriya Nambiar ◽  
Poornima Manjrekar ◽  
Asavari Desai ◽  
Pramod Philip
Keyword(s):  
Orthodontic Treatment ◽  
Gingival Crevicular Fluid ◽  
Application Site ◽  
Coil Spring ◽  
Time Intervals ◽  
Elisa Kit ◽  
Force Application ◽  
Crevicular Fluid ◽  
Orthodontic Forces

To assess the change in the myeloperoxidase levels(MPO)in the gingival crevicular fluid(GCF) at different time intervals during the course of orthodontic treatment with the application of intermittent and continuous forces. It was an interventional, split mouth study done on twenty patients scheduled to undergo fixed orthodontic treatment with therapeutic extractions of the first premolars.Mini implant assisted enmasse retraction was done after leveling and aligning of the arch.Continuous force with the help of Niti coil spring was used in the first quadrant and intermittent force with an elastomeric chain was used in the second quadrant. MPO was collected from the GCF using What man’s paper at baseline,1 week and 2 weeks after the application of retraction forces.The levels were assessed using a commercially available ELISA kit. Mean MPO activity was increased in the GCF of all the patients at 1 and 2 weeks after retraction,in both quadrants.At 2 weeks, MPO levels at the intermittent force application site were found to be significantly higher than those at the site of continuous force application. Myeloperoxidase is a useful marker to assess inflammation associated with orthodontic tooth movement.Intermittent forces cause a higher degree of inflammation as compared to continuous forces.

Gingival Health of Porcelain Laminate Veneered Teeth: A Retrospective Assessment

2019 ◽  
Vol 44 (5) ◽  
pp. 452-458 ◽  
Author(s):  
R Arif ◽  
JB Dennison ◽  
D Garcia ◽  
P Yaman
Keyword(s):  
Repeated Measures ◽  
Gingival Crevicular Fluid ◽  
Statistical Significance ◽  
Fluid Collection ◽  
Gingival Recession ◽  
Gingival Health ◽  
Long Term Effect ◽  
The Mean ◽  
Crevicular Fluid

SUMMARY Statement of Problem: The long-term effect of the presence of porcelain laminate veneers (PLVs) on the health of the surrounding gingival issues is not available in the restorative literature. Purpose: To assess the long-term effect of PLVs on the health of the surrounding gingival tissues. A secondary aim was to correlate gingival crevicular fluid (GCF) scores with clinical parameters used for gingival health assessment in teeth treated with PLVs. Methods and Materials: Patients who received PLVs placed at the Graduate Restorative Clinic within a seven- to 14-year period were recalled for clinical evaluations. Periodontal measurements including gingival index (GI), periodontal pocket depth (PPD), gingival recession (GR), and clinical attachment level (CAL) were measured using a standard probe and indices. Gingival Crevicular Fluid (GCF) was measured with a Periotron machine (Periotron 8000, Oraflow Inc), using Periopaper (Periopaper Gingival Fluid Collection Strip, Oraflow Inc.) for fluid collection. Photographs of any observed clinical defect were taken. Data were tabulated using Excel 2010 (Microsoft Corp). Statistical analysis for all descriptive statistics was performed using SPSS 21 (SPSS Software, IBM Corp.) and Stata SE 13 (Stata Software, StataCorp). Repeated-measures analysis of variance (ANOVA) was done to test for statistical significance of the mean pocket depths between the restored and unrestored surfaces of the veneered teeth. The significance level for all tests was p<0.05. Pearson's correlation coefficient was performed for testing statistical significance between GCF and GI and between GCF and PPD. Results: The frequency distribution of the GI included 47 PLVs (43%) with normal gingiva, 16 (15%) with mild inflammation, and 46 (42%) with moderate inflammation and bleeding on probing. The average PPD on the facial surface of the maxillary and mandibular PLVs was 2.17 mm and 2.16 mm, respectively. On the lingual surface, the average PPD was 2.10 mm for maxillary and 2.22 mm for mandibular PLVs. Gingival recession was seen in 27% of the evaluated PLVs. The repeated-measures ANOVA revealed p≥0.136, showing no statistical difference in the mean pocket depths between restored facial and unrestored lingual surfaces of the veneered teeth. A moderate correlation (r=0.407) was found between GCF and GI, which was significant at p<0.001. No correlation (r=0.124) was found between GCF and PPD, which was not significant at p=0.197. Conclusions: Gingival response to the evaluated PLVs was in the satisfactory range, with overall GI scores ranging between normal and moderate inflammation, pocket depths ranging from 1 to 2 mm, and recession present in 27% of the evaluated PLVs. No statistically significant difference was found between the mean pocket depths of the restored and unrestored surfaces of veneered teeth (p≥0.136). A moderate correlation was found between GCF and GI.

scholarly journals Evaluation of IL1β and IL6 Gingival Crevicular Fluid Levels during the Early Phase of Orthodontic Tooth Movement in Adolescents and Young Adults

2021 ◽  
Vol 11 (2) ◽  
pp. 521
Author(s):  
Simina Chelărescu ◽  
Petra Șurlin ◽  
Mioara Decusară ◽  
Mădălina Oprică ◽  
Eugen Bud ◽  
...  
Keyword(s):  
Young Adults ◽  
Orthodontic Treatment ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Early Stage ◽  
Statistical Tests ◽  
Orthodontic Tooth Movement ◽  
Adolescents And Young Adults ◽  
Periodontal Tissues ◽  
Crevicular Fluid

Background: The crevicular fluid analysis represents a useful diagnosis tool, with the help of which noninvasive cellular metabolic activity can be analyzed. The aim of the study is to investigate comparatively IL1β and IL6 in the gingival crevicular fluid of clinically healthy adolescents and young adults during the acute phase of orthodontic treatment. Methods: Gingival crevicular fluid was collected from 20 patients (aged between 11 and 28) undergoing orthodontic treatment. Measurements were taken before (T0) and after 24 h after distalization forces were activated (T1). IL1β and IL 6 were analyzed using Elisa tests. The statistical tests used were two-sided t tests. Results: Between the two time periods there was a significant raise both in the crevicular fluid rate (0.57 µL at T0 vs. 0.95 µL at T1, p = 0.001) and in IL1β levels (15.67 pg/µL at T0 vs. 27.94 pg/µL at T1, p = 0.009). We were able to identify IL6 only in a third of the sites. There is a significantly increased level of ILβ at T1 in adolescents, more than in young adults (42.96 pg/µL vs. 17.93 pg/µL, p = 0.006). Conclusions: In the early stage of orthodontic treatment, the periodontal tissues of adolescents are more responsive to orthodontic forces than those of young adults.

scholarly journals Are Proteinase 3 and Cathepsin C Enzymes Related to Pathogenesis of Periodontitis?

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Oya Türkoğlu ◽  
Elif Azarsız ◽  
Gülnur Emingil ◽  
Necil Kütükçüler ◽  
Gül Atilla
Keyword(s):  
Gingival Crevicular Fluid ◽  
Periodontal Diseases ◽  
Study Groups ◽  
Aggressive Periodontitis ◽  
Control Group ◽  
Proteinase 3 ◽  
Cathepsin C ◽  
Probing Depth ◽  
Inflammatory Processes ◽  
Crevicular Fluid

Aim. Cathepsin C is the activator of the polymorphonuclear leukocyte-derived proteinase 3, which contributes to inflammatory processes. The aim of the present study was to investigate gingival crevicular fluid (GCF) proteinase 3 and cathepsin C levels in periodontal diseases.Design. Eighteen patients with chronic periodontitis (CP), 20 patients with generalized aggressive periodontitis (G-AgP), 20 patients with gingivitis, and 18 healthy subjects were included in the study. Periodontal parameters including probing depth, clinical attachment level, papilla bleeding index, and plaque index were assessed in all study subjects. GCF proteinase 3 and cathepsin C levels were analyzed by ELISA.Results. GCF proteinase 3 total amount was significantly higher in diseased groups compared to control group, after adjusting ageP<0.05. No differences were found in GCF cathepsin C levels among the study groupsP>0.05. Periodontal parameters of sampling sites were positively correlated with GCF proteinase 3 total amountsP<0.01but not with cathepsin C total amountsP>0.05.Conclusions. Elevated levels of GCF proteinase 3 in CP, G-AgP, and gingivitis might suggest that proteinase 3 plays a role during inflammatory periodontal events in host response. However, cathepsin C in GCF does not seem to have an effect on the pathogenesis of periodontal diseases.

scholarly journals Comparative Evaluation of C-reactive Proteins in Pregnant Women with and without Periodontal Pathologies: A Prospective Cohort Analysis

2016 ◽  
Vol 17 (6) ◽  
pp. 480-483
Author(s):  
Padmakanth Mannava ◽  
Sunil Gokhale ◽  
Sudarshan Pujari ◽  
Krishna P Biswas ◽  
Satish Kaliappan ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Prospective Cohort ◽  
Comparative Evaluation ◽  
Periodontal Diseases ◽  
Cohort Analysis ◽  
Study Groups ◽  
Periodontal Index ◽  
The Mean ◽  
Adverse Pregnancy ◽  
Two Parameters

ABSTRACT Introduction Inflammation of tooth supporting structures is referred to as periodontitis. C-reactive proteins (CRP) levels are usually increased in case of chronic inflammatory process like periodontitis. Association of CRP with pregnancy has been observed in the past, which includes most commonly preterm delivery, preeclampsia, etc. Therefore, it can be hypothesized that CRP may act as a link between periodontitis and adverse pregnancy outcomes. Hence, we aim to evaluate the plasma CRP levels in pregnant women with and without periodontal pathologies. Materials and methods The study included 210 pregnant women who reported to the hospital with periodontal problems and for routine checkups. All the patients were divided into three groups based on the presence and absence of periodontal pathologies. Russell's Periodontal Index Score was used for the evaluation of periodontal status of the subjects. Results While comparing the mean CRP levels in all the three study groups, statistically significant results were obtained. Statistically significant results were obtained while comparing the mean CRP levels in group C patients before treatment and after treatment therapy. The CRP levels were estimated by taking blood samples. Paired t-test and one-way analysis of variance was used to assess the correlation between the two parameters. Conclusion Casual association might exist between the CRP levels and periodontal diseases in pregnant women and the CRP levels may also get elevated in pregnant women. How to cite this article Mannava P, Gokhale S, Pujari S, Biswas KP, Kaliappan S, V Shashank. Comparative Evaluation of C-reactive Proteins in Pregnant Women with and without Periodontal Pathologies: A Prospective Cohort Analysis. J Contemp Dent Pract 2016;17(6):480-483.

Sign in / Sign up

Export Citation Format

Share Document