Investigation of antiproliferative effects of gossypin on lung cancer cell line

2021 ◽  
pp. 37-40
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Anticancer Agents ◽  
A549 Cells ◽  
Supportive Therapy ◽  
Cancer Cell Line ◽  
Inhibitory Effect ◽  
Lung Cancer Cell Line ◽  
Cancer Treatments ◽  
Mtt Method

Purpose: The rapid growth, morbidity and mortality of lung cancer and the lack of effective treatment have attracted great interest from researchers to find new cancer treatments aimed at the effect of gossypine on cell proliferation and apoptosis of A549 cells. The most used of these products are flavonoids. Gossypin is a potential chemo preventive and therapeutic agent for lung cancer. Material and Method: We investigated the effect of Gossypin anticancer activity on A549 cell proliferation with MTT method, depending on dose and time. In addition, mRNA expression levels of the apoptotic markers caspase-3 (CAS-3) and caspase-9 (CAS-9) were investigated by real-time PCR. In our study, six groups were used as control, gossypin (10, 20, 40 μM), cisplatin 5 μg/mL and cisplatin 5 μg/mL+gossypin 40 μM combine concentrations. The proliferative and antiapoptotic effects of gossypin at 24, 48 and 72 hours were investigated. Results were analyzed and presented as cell viability (%). Results: In our results, it was determined that gossypin especially at a dose of 40 μM and at 72 hours showed almost as much effect on A549 cells, but the highest inhibitory effect was seen in the 40 combined group of cisplatin 5 μg / mL + gossypin. In addition, gossypin caused a significant increase in apoptosis genes (CASP-3, CASP-9) compared to control. Conclusion: Our study showed that gossypin significantly increases the chemosensitivity of cisplatin. Based on this, it is predicted that gossypin can be used as a supportive therapy that increases the effectiveness of anticancer agents. However, more detailed research should be done for this.

scholarly journals The Effects of 2,2',4'-Trihydroxychalcone on the Cell Proliferation, Metastasis, and Apoptosis in Human Lung Cancer Cell Line A549

2021 ◽  
Author(s):  
Jialin Sun ◽  
Zhanqi Cao ◽  
Shiwei Sun ◽  
Zhonghua Sun ◽  
Shuhong Sun ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Human Lung ◽  
Vasculogenic Mimicry ◽  
A549 Cells ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Human Lung Cancer ◽  
Lung Cancer Cell ◽  
Related Proteins

Abstract Objectives：Our study aimed to evaluate the antitumor effects of 2,2',4'-trihydroxychalcone (7a) on human lung cancer cell line A549. Methods：A549 cells were treated with different concentrations of 7a for different times. The cells without 7a were set as the negative control group. The cell proliferation, invasion, vasculogenic mimicry (VM) formation, heterogeneous adhesion, apoptosis were, respectively, measured by CCK-8, transwell invasion assay, vasculogenic mimicry assay, adhesion assay and flow cytometry. In addition, the expression of related proteins were examined via western blot or ELISA. Results：Our research found that 7a had a significant inhibitory effect on the survival rate of lung cancer A549 cells, while almost had no effect on human lung epithelial BEAS-2B cells and human venous endothelial cells (HUVECs). The migration rate, VM length, invasion and heterogeneous adhesion number of cells treated with 7a significantly decreased as the increase in concentration, while the apoptosis rate increased. Western blot analysis showed that 7a treatment significantly upregulated the expression of E-cadherin, cleaved PARP, Bax, caspase-3, and simultaneously downregulated the expression of MMP-2/9, Bcl-2, p-PI3K, p-Akt, p-MTOR, VEGF, E-selectin and N-cadherin. At the same time, ELISA results found that the pro-angiogenic factor VEGF level in culture media was reduced in the presence of 7a. Additionally, 7a could also reduce the nucleus NF-κB protein level, which would inhibit gene transcription of tumor activity-related proteins. Conclusion：7a might exert inhibitory effects on A549 cells via inhibiting cell proliferation, migration, VM formation, heterogeneous adhesion and inducing apoptosis through suppressing the PI3K/AKT/NF-κB signaling pathway, suggesting that 7a might have therapeutic potential for the treatment of lung cancer.

scholarly journals Potential of Thai Herbal Extracts on Lung Cancer Treatment by Inducing Apoptosis and Synergizing Chemotherapy

Molecules ◽  
2020 ◽  
Vol 25 (1) ◽  
pp. 231 ◽  
Author(s):  
Juthathip Poofery ◽  
Patompong Khaw-on ◽  
Subhawat Subhawa ◽  
Bungorn Sripanidkulchai ◽  
Apichat Tantraworasin ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Ethyl Acetate ◽  
Cancer Treatment ◽  
Apoptotic Cell ◽  
Primary Lung Cancer ◽  
A549 Cells ◽  
Cancer Cell Line ◽  
Ethanolic Extract ◽  
Lung Cancer Cell Line ◽  
Lung Cancer Treatment

The incidence of lung cancer has increased while the mortality rate has continued to remain high. Effective treatment of this disease is the key to survival. Therefore, this study is a necessity in continuing research into new effective treatments. In this study we determined the effects of three different Thai herbs on lung cancer. Bridelia ovata, Croton oblongifolius, and Erythrophleum succirubrum were extracted by ethyl acetate and 50% ethanol. The cytotoxicity was tested with A549 lung cancer cell line. We found four effective extracts that exhibited toxic effects on A549 cells. These extracts included ethyl acetate extracts of B. ovata (BEA), C. oblongifolius (CEA), and E. succirubrum (EEA), and an ethanolic extract of E. succirubrum (EE). Moreover, these effective extracts were tested in combination with chemotherapeutic drugs. An effective synergism of these treatments was found specifically through a combination of BEA with methotrexate, EE with methotrexate, and EE with etoposide. Apoptotic cell death was induced in A549 cells by these effective extracts via the mitochondria-mediated pathway. Additionally, we established primary lung cancer and normal epithelial cells from lung tissue of lung cancer patients. The cytotoxicity results showed that EE had significant potential to be used for lung cancer treatment. In conclusion, the four effective extracts possessed anticancer effects on lung cancer. The most effective extract was found to be E. succirubrum (EE).

New Procaspase Activating Compound (PAC-1) Like Molecules as Potent Antitumoral Agents Against Lung Cancer

2019 ◽  
Vol 16 (6) ◽  
pp. 645-655
Author(s):  
Leyla Yurttaş ◽  
Ömer Öztürk ◽  
Zerrin Cantürk
Keyword(s):  
Lung Cancer ◽  
Antitumor Activity ◽  
Cell Line ◽  
Human Lung ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Human Lung Cancer ◽  
Mtt Method ◽  
Acyl Hydrazone ◽  
Analysis System

Background: In this study, novel ortho-hydroxy N-acyl hydrazone moiety including compounds (3a-l) were designed, based on procaspase activating compound (PAC-1) which is a small molecule known with antitumor activity. The antitumor activity was evaluated on A549 (human lung cancer cell line) and CCD 19Lu (human lung normal cell line). Methods: Twelve N'-arylidene-2-[4-(methylsulfonyl)piperazin-1-yl]acetohydrazide derivatives (3a-l) were synthesized starting from ethyl 1-piperazinylacetate. All compounds were tested using MTT method and Xcelligence-Real time cell analysis system (RTCA DP) to determine their antitumor activity. Results: Some physicochemical properties of four active compounds were also predicted using MolSoft, PreADMET and PROTOX software. Four of them, 3h, 3j, 3k and 3l bearing 3-hydroxy, 4-dimethylamino, 2,6-dichloro and 3,4-dichloro substituents in order exhibited selective cytotoxicity. Conclusion: Eligible values were obtained in the specified ranges as to be an oral/intravenous drug considering the physicochemical calculations.

Effect of UbcH10 gene silencing on cell proliferation and cell cycle of lung cancer cell line NCI-H226

2013 ◽  
Vol 32 (6) ◽  
pp. 608-612
Author(s):  
Li-ming ZHAO ◽  
Guang-yuan SUN ◽  
Li-rong LOU ◽  
Liang-zhe WANG ◽  
Zheng FANG ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Cycle ◽  
Cell Proliferation ◽  
Gene Silencing ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Lung Cancer Cell

scholarly journals Anticancer Potentials of Root Extract ofPolygala senegaand Its PLGA Nanoparticles-Encapsulated Form

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Saili Paul ◽  
Soumya Sundar Bhattacharyya ◽  
Naoual Boujedaini ◽  
Anisur Rahman Khuda-Bukhsh
Keyword(s):  
Lung Cancer ◽  
A549 Cells ◽  
Cancer Cell Line ◽  
Ethanolic Extract ◽  
Aqueous Dispersion ◽  
Plga Nanoparticles ◽  
Lung Cancer Cell Line ◽  
Normal Lung ◽  
Root Extract ◽  
Induced Apoptosis

Ethanolic extract ofPolygala senega(EEPS) had little or no cytotoxic effects on normal lung cells, but caused cell death and apoptosis to lung cancer cell line A549. In the present paper, ethanolic root extract ofP. senega(EEPS) was nanoencapsulated (size: 147.7 nm) by deploying a biodegradable poly-(lactic-co-glycolic) acid (PLGA). The small size of the NEEPS resulted in an enhanced cellular entry and greater bioavailability. The growth of cancer cells was inhibited better by NEEPS than EEPS. Both EEPS and NEEPS induced apoptosis of A549 cells, which was associated with decreased expression of survivin, PCNA mRNA, and increased expression of caspase-3, p53 mRNAs of A549 cells. The results show that the anticancer potential of the formulation of EEPS-loaded PLGA nanoparticles was more effective than EEPS per se, probably due to more aqueous dispersion after nanoencapsulation. Therefore, nanoencapsulated ethanolic root extract ofP. senegamay serve as a potential chemopreventive agent against lung cancer.

scholarly journals CyclinD1 antisense oligonucleotide induces apoptosis of lung adenocarcinoma cell A549

2020 ◽  
Author(s):  
Tahama Sharma
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Lung Adenocarcinoma ◽  
Antisense Oligonucleotide ◽  
A549 Cells ◽  
Vascular Endothelial ◽  
Adenocarcinoma Cell ◽  
Mtt Method ◽  
Induced Apoptosis ◽  
Endothelial Growth Factor

To investigate the role of CyclinD1 antisense oligonucleotide in lung cancer gene therapy, an expression vector containing CyclinD1 antisense oligonucleotide, named pcDNA3.1-CyclinD1, was designed, and this vector was transfected into lung adenocarcinoma cell A549. After G418 screening, A549 cells stably expressing CyclinD1 antisense oligonucleotide were obtained. Cell proliferation was detected by MTT method, and apoptosis was detected by flow cytometry. The results showed that CyclinD1 antisense was stably transfected. After the oligonucleotide transfection, A549 cell proliferation was significantly inhibited, and apoptosis was increased. To further investigate the mechanism of CyclinD1 antisense oligonucleotide-induced apoptosis, Western blot was utilized to measure intracellular retinoblastoma protein (pRb), adenovirus E2 factor-1 (E2F-1), vascular endothelial growth factor (VEGF), and matrix metalloproteinase (MMP) -2 and MMP-9 expression. After transfection with CyclinD1 antisense oligonucleotide, the expression of pRb, E2F-1, VEGF, MMP-2, and MMP-9 proteins in A549 cells was significantly reduced. The abovementioned results indicate that CyclinD1 antisense oligonucleotide can cause apoptosis of lung cancer cells, and its mechanism may be related to the decreased expression of pRb, E2F-1, VEGF, MMP-2, and MMP-9.

scholarly journals CHARACTERIZATION OF THE CYTOTOXIC EFFECTS OF THE COMBINATION OF CISPLATIN AND FLAVANOL (-)-EPICATECHIN ON HUMAN LUNG CANCER CELL LINE A549. AN ISOBOLOGRAPHIC APPROACH

2018 ◽  
Vol 40 (1) ◽  
pp. 19-23 ◽  
Author(s):  
O Varela-Castillo ◽  
P Cordero ◽  
G Gutiérrez-Iglesias ◽  
I Palma ◽  
I Rubio-Gayosso ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Inhibitory Effect ◽  
Lung Cancer Cell Line ◽  
Human Lung Cancer ◽  
Response Curves ◽  
Isobolographic Analysis ◽  
Therapeutic Compounds

Background: Among malignancies, lung cancer is a leading cause of death. Platinum-based therapeutic compounds used to treat lung cancer have not been able to increase the survival of patients and such compounds have a high incidence of adverse and toxic effects. It has been proposed that flavonoids such as catechins may significantly reduce the risk of developing cancer, alongside with other health benefits. The aim of this work was to determine the effect of (-)-epicatechin, the main flavanol found in cocoa, on the proliferation of the lung non-small cell adenocarcinoma cancer cell line A549, and to determine its effects when added simultaneously with cisplatin. Materials and Methods: Concentration-response curves for cisplatin and epicatechin were obtained, inhibitory concentrations calculated and an isobolographic analysis was then performed. Results: We found that epicatechin has a concentration-dependent inhibitory effect on proliferation of tumor cells and the isobolographic analysis reveals that the effect of its combination with cisplatin is synergistic. It was also observed that epicatechin promotes cell death by apoptosis. Conclusions: Epicatechin might be considered for future studies to explore its possible use as coadjuvant in cisplatin-based treatments.

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