scholarly journals Potential of Thai Herbal Extracts on Lung Cancer Treatment by Inducing Apoptosis and Synergizing Chemotherapy

Molecules ◽  
2020 ◽  
Vol 25 (1) ◽  
pp. 231 ◽  
Author(s):  
Juthathip Poofery ◽  
Patompong Khaw-on ◽  
Subhawat Subhawa ◽  
Bungorn Sripanidkulchai ◽  
Apichat Tantraworasin ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Ethyl Acetate ◽  
Cancer Treatment ◽  
Apoptotic Cell ◽  
Primary Lung Cancer ◽  
A549 Cells ◽  
Cancer Cell Line ◽  
Ethanolic Extract ◽  
Lung Cancer Cell Line ◽  
Lung Cancer Treatment

The incidence of lung cancer has increased while the mortality rate has continued to remain high. Effective treatment of this disease is the key to survival. Therefore, this study is a necessity in continuing research into new effective treatments. In this study we determined the effects of three different Thai herbs on lung cancer. Bridelia ovata, Croton oblongifolius, and Erythrophleum succirubrum were extracted by ethyl acetate and 50% ethanol. The cytotoxicity was tested with A549 lung cancer cell line. We found four effective extracts that exhibited toxic effects on A549 cells. These extracts included ethyl acetate extracts of B. ovata (BEA), C. oblongifolius (CEA), and E. succirubrum (EEA), and an ethanolic extract of E. succirubrum (EE). Moreover, these effective extracts were tested in combination with chemotherapeutic drugs. An effective synergism of these treatments was found specifically through a combination of BEA with methotrexate, EE with methotrexate, and EE with etoposide. Apoptotic cell death was induced in A549 cells by these effective extracts via the mitochondria-mediated pathway. Additionally, we established primary lung cancer and normal epithelial cells from lung tissue of lung cancer patients. The cytotoxicity results showed that EE had significant potential to be used for lung cancer treatment. In conclusion, the four effective extracts possessed anticancer effects on lung cancer. The most effective extract was found to be E. succirubrum (EE).

scholarly journals Anticancer Potentials of Root Extract ofPolygala senegaand Its PLGA Nanoparticles-Encapsulated Form

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Saili Paul ◽  
Soumya Sundar Bhattacharyya ◽  
Naoual Boujedaini ◽  
Anisur Rahman Khuda-Bukhsh
Keyword(s):  
Lung Cancer ◽  
A549 Cells ◽  
Cancer Cell Line ◽  
Ethanolic Extract ◽  
Aqueous Dispersion ◽  
Plga Nanoparticles ◽  
Lung Cancer Cell Line ◽  
Normal Lung ◽  
Root Extract ◽  
Induced Apoptosis

Ethanolic extract ofPolygala senega(EEPS) had little or no cytotoxic effects on normal lung cells, but caused cell death and apoptosis to lung cancer cell line A549. In the present paper, ethanolic root extract ofP. senega(EEPS) was nanoencapsulated (size: 147.7 nm) by deploying a biodegradable poly-(lactic-co-glycolic) acid (PLGA). The small size of the NEEPS resulted in an enhanced cellular entry and greater bioavailability. The growth of cancer cells was inhibited better by NEEPS than EEPS. Both EEPS and NEEPS induced apoptosis of A549 cells, which was associated with decreased expression of survivin, PCNA mRNA, and increased expression of caspase-3, p53 mRNAs of A549 cells. The results show that the anticancer potential of the formulation of EEPS-loaded PLGA nanoparticles was more effective than EEPS per se, probably due to more aqueous dispersion after nanoencapsulation. Therefore, nanoencapsulated ethanolic root extract ofP. senegamay serve as a potential chemopreventive agent against lung cancer.

Investigation of antiproliferative effects of gossypin on lung cancer cell line

2021 ◽  
pp. 37-40
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Anticancer Agents ◽  
A549 Cells ◽  
Supportive Therapy ◽  
Cancer Cell Line ◽  
Inhibitory Effect ◽  
Lung Cancer Cell Line ◽  
Cancer Treatments ◽  
Mtt Method

Purpose: The rapid growth, morbidity and mortality of lung cancer and the lack of effective treatment have attracted great interest from researchers to find new cancer treatments aimed at the effect of gossypine on cell proliferation and apoptosis of A549 cells. The most used of these products are flavonoids. Gossypin is a potential chemo preventive and therapeutic agent for lung cancer. Material and Method: We investigated the effect of Gossypin anticancer activity on A549 cell proliferation with MTT method, depending on dose and time. In addition, mRNA expression levels of the apoptotic markers caspase-3 (CAS-3) and caspase-9 (CAS-9) were investigated by real-time PCR. In our study, six groups were used as control, gossypin (10, 20, 40 μM), cisplatin 5 μg/mL and cisplatin 5 μg/mL+gossypin 40 μM combine concentrations. The proliferative and antiapoptotic effects of gossypin at 24, 48 and 72 hours were investigated. Results were analyzed and presented as cell viability (%). Results: In our results, it was determined that gossypin especially at a dose of 40 μM and at 72 hours showed almost as much effect on A549 cells, but the highest inhibitory effect was seen in the 40 combined group of cisplatin 5 μg / mL + gossypin. In addition, gossypin caused a significant increase in apoptosis genes (CASP-3, CASP-9) compared to control. Conclusion: Our study showed that gossypin significantly increases the chemosensitivity of cisplatin. Based on this, it is predicted that gossypin can be used as a supportive therapy that increases the effectiveness of anticancer agents. However, more detailed research should be done for this.

Development and Biological Evaluation of Imidazothiazole propenones as Tubulin Inhibitors that Effectively Triggered Apoptotic Cell Death in Alveolar Lung Cancer Cell Line

2017 ◽  
Vol 2 (22) ◽  
pp. 6480-6487 ◽  
Author(s):  
Ibrahim Bin Sayeed ◽  
Koteswara Rao Garikapati ◽  
Venkata Krishna Kanth Makani ◽  
Apoorva Nagarajan ◽  
Mohd Adil Shareef ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Death ◽  
Cell Line ◽  
Cancer Cell ◽  
Apoptotic Cell ◽  
Cancer Cell Line ◽  
Biological Evaluation ◽  
Lung Cancer Cell Line ◽  
Lung Cancer Cell ◽  
Tubulin Inhibitors

Changes in Lung Cancer Cell Line Affected by Cytotoxicity of Lagenaria Siceraria Plant Extract

2021 ◽  
Vol 15 (5) ◽  
pp. 1282-1284
Author(s):  
Moein Shaneh
Keyword(s):  
Lung Cancer ◽  
Side Effects ◽  
Cancer Treatment ◽  
Cancer Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Lagenaria Siceraria ◽  
Lung Cancer Cell

Chemotherapy is a type of cancer treatment in which the lack of selective cytotoxicity often leads to intolerable side effects. Today, the use of medicinal plants is essential in treating cancer due to their fewer side effects. Lagenaria siceraria Standl is critical for cytotoxicity studies due to its polyphenolic, cucurbitacins, pectin, flavonoids, and saponin compounds. In this study, the cytotoxic effects of plant fruit extract were investigated on lung cancer cell lines. To this end, the hydroalcoholic extract of the plant fruit was initially prepared by the percolation method. Then, the effects of solutions containing samples with different concentrations (5000, 500, 1000, 100, 100, 250, 10, 1, 0.1μg.ml-1) were investigated by MTT assay on lung cancer cell line (A549). Cisplatin was considered as a positive control. Statistical calculations were carried out using Prism V.3 software to compare IC50, and the data were analyzed by analysis of variance (ANOVA) and t-test. The results indicated that the IC50 level of cisplatin anti-cancer drug, as a common drug in the market, is significantly lower than Lagenaria siceraria extract. However, the extract of this plant revealed a significant growth inhibitory effect on lung cancer cells. The results also showed that Lagenaria siceraria extract is an effective cytotoxic compound on lung cancer cells. More extensive studies are needed to find effective plant extracts compounds to find and design new and effective cancer treatment drugs. Keywords: Lagenaria siceraria, Cell line, Lung cancer, IC50, MTTassay

Primary lung cancer: treatment with radio-frequency thermal ablation

2004 ◽  
Vol 14 (5) ◽  
pp. 897-901 ◽  
Author(s):  
L. Thanos ◽  
S. Mylona ◽  
M. Pomoni ◽  
V. Kalioras ◽  
L. Zoganas ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Radio Frequency ◽  
Cancer Treatment ◽  
Thermal Ablation ◽  
Primary Lung Cancer ◽  
Lung Cancer Treatment

scholarly journals Melissa officinalis L. ethanolic extract inhibits the growth of a lung cancer cell line by interfering with the cell cycle and inducing apoptosis

2018 ◽  
Vol 9 (6) ◽  
pp. 3134-3142 ◽  
Author(s):  
D. B. Magalhães ◽  
I. Castro ◽  
V. Lopes-Rodrigues ◽  
J. M. Pereira ◽  
L. Barros ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Cycle ◽  
Cell Line ◽  
Mediterranean Region ◽  
Cancer Cell Line ◽  
Ethanolic Extract ◽  
Lung Cancer Cell Line ◽  
Lung Cancer Cell ◽  
Melissa Officinalis ◽  
The Family

Melissa officinalis is a plant from the family Lamiaceae, native in Europe particularly in the Mediterranean region.

The effect of antisense oligodeoxynucleotides targeting Aurora A kinase on cell proliferation and chemosensitivity to paclitaxel in human lung cancer cell line A549

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 21147-21147
Author(s):  
R. Meng ◽  
G. Wu ◽  
K. Y. Yang ◽  
J. Cheng
Keyword(s):  
Lung Cancer ◽  
Cell Line ◽  
A549 Cells ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Human Lung Cancer ◽  
Aurora A ◽  
Lung Cancer Cell ◽  
Mrna And Protein Expression ◽  
Cell Inhibition

21147 Background: Aurora kinases representing a family of evolutionarily conserved mitotic serine/threonine kinases have been found elevated in lung andenocarcinoma cell line A549. It is suggested that the overexpression of Aurora A contributes to the carcinogenesis, chromosomal instability (CIN), and de-differentiation of lung cancers. To address its possibility as a therapeutic target for lung cancer, we employed the antisense oligodeoxynucleotide (ASODN) technique to inhibit Aurora A expression and investegate its effect on tumor growth and cell cycle of A549, as well as the chemosensitivity of paclitaxel. Methods: Aurora A ASODN was synthesized and transfected into A549 cells by lipofectAMINE 2000.Aurora A mRNA and protein expression were examined by reverse transcription -polymerase chain reaction (RT-PCR) and Western blot respectively.Cell cycle distribution was observed by flow cytometer.MTT assay was used to evaluate cell inhibition ratio before and after transfection. Results: The proliferation of the A549 cells was inhibited by Aurora A ASODN dose and time dependently. It was also observed that the IC50 of A549 cells after 48 hours’ treatment of ASODN was about 300nmol/L and under such circumstances, the Aurora A mRNA and protein expression significantly decreased (P<0.05), along with the induction of accumulation of cells in S phase and the G2-M transition. Furhermore, cell inhibition ratio of the combination of Aurora A ASODN and paclitaxel was higher significantly than paclitaxel(P<0.05) or Aurora A ASODN alone (P<0.05). Conclusions: Inhibition of Aurora A expression can results in the suppression of cell growth and chemosensitizing activity to paclitaxel in human lung cancer cell line A549. No significant financial relationships to disclose.

Sign in / Sign up

Export Citation Format

Share Document