scholarly journals In vitro antioxidant, 𝛼-amylase and 𝛼-glucosidase activities of methanol extracts from three Momordica species

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Olubunmi Adenike Akinwunmi
Keyword(s):  
Metal Ion ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Total Phenolic ◽  
Limited Information ◽  
Glucosidase Inhibitors ◽  
Dpph Scavenging ◽  
Oral Hypoglycemic Drugs ◽  
Metal Ion Chelation

<p>Antioxidant based drug preparations are used in the prevention and management of complex diseases which include atherosclerosis, stroke, diabetes, alzheimer’s disease and cancer. Diabetes mellitus is a metabolic disorder of glucose metabolism. The management of blood glucose level is the hallmark in the treatment of this ailment, which may be achieved through the use of oral hypoglycemic drugs such as biguanides, insulin secretagogues, and 𝛼-amylase and glucosidase inhibitors. Although several biological activities had been reported for  <em>Momordica</em> <em>foetida </em>and <em>Momordica</em> <em>charantia</em>; it appears there is limited information on the  biological activity of <em>Momordica</em> <em>cissoides</em>. The purpose of this study is to compare the antioxidant, 𝛼-amylase and 𝛼-glucosidase inhibitory activities of <em>Momordica</em> <em>charantia </em>(M1), <em>Momordica</em> <em>foetida </em>(M2)<em>, </em>and <em>Momordica</em> <em>cissoides</em>  (M3) to establish a chemotaxonomic relationship between them. The antioxidant activities measured by DPPH scavenging properties, metal ion chelation, hydrogen peroxide scavenging and ABTS revealed that <em>M. foetida</em> had the highest inhibition potential, followed by <em>M. charantia</em> and the least being <em>M. cissoides</em>. In contrast, the antioxidant activities measured by FRAP,  the total phenolic content , flavonoids and tannins revealed that <em>M. cissoides</em> had the best antioxidant potential, while <em>M. foetida</em> had the least activity. The comparative α-amylase and glucosidase inhibitory studies performed demonstrated that the extracts of <em>M.cissoides </em>had the highest inhibitory potentials. Thus, the plant can be used in the management of diabetes.</p>

scholarly journals Assessment of in vitro Antioxidant and Antidiabetic Capacities of Medlar (Mespilus germanica)

2018 ◽  
Vol 47 (2) ◽  
pp. 384-389
Author(s):  
Sebnem Selen ISBILIR ◽  
Sevilay Inal KABALA ◽  
Hulya YAGAR
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Flower Bud ◽  
Ethanol Extracts ◽  
Dpph Scavenging ◽  
Β Carotene

The objective of the current study was to evaluate the antioxidant activity and enzyme inhibitory effect of different parts of medlar including fruit, leaf and flower bud by using various in vitro methods, and also determination of total phenolic and flavonoid content in the samples. Ethanol extracts of medlar parts were prepared and their antioxidant activities were determined using 1,1-diphenyl-2-picryl-hydrazil (DPPH•) scavenging and β-carotene bleaching methods. The leaf extract showed the strongest antioxidant activity. DPPHradical scavenging activity was in the order of BHA > leaf > bud > fruit. This ordering was the same for β-carotene bleaching activity, tocopherol > leaf > bud > fruit. The highest total phenolic (60.3 ± 1.69 mg GAE g-1 extract) and flavonoid (14.77 ± 1.15 mg QE g-1 extract) content were determined in leaf extract. For possible antidiabetic effects of extracts, α-amylase and α-glucosidase inhibitory activities were investigated, the bud extract showed the highest inhibition activities among the all extracts.

scholarly journals In vitro antioxidant activity of stem bark of Trichilia catigua Adr. Juss

2012 ◽  
Vol 62 (3) ◽  
pp. 371-382 ◽  
Author(s):  
Jean Paul Kamdem ◽  
Sílvio Terra Stefanello ◽  
Aline Augusti Boligon ◽  
Caroline Wagner ◽  
Ige Joseph Kade ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
High Concentrations ◽  
Dpph Scavenging

Antioxidant activity of the ethanolic extract and fractions from the stem bark of T. catigua was investigated. IC50 (for DPPH scavenging) by T. catigua varied from 9.17 ± 0.63 to 76.42 ± 5.87 mg mL-1 and total phenolic content varied from 345.63 ± 41.08 to 601.27 ± 42.59 mg GAE g-1 of dry extract. Fe2+-induced lipid peroxidation was significantly reduced by the ethanolic extract and fractions. Mitochondrial Ca2+-induced dichlorofluorescein oxidation was significantly reduced by the ethanolic extract in a concentration-dependent manner. Ethanolic extract reduced mitochondrial Dym only at high concentrations (40-100 mg mL-1), which indicates that its toxicity does not overlap with its antioxidant effects. Results suggest involvement of antioxidant activities of T. catigua in its pharmacological properties.

scholarly journals Scrophularia Tenuipes Coss and Durieu: Phytochemical Composition and Biological Activities

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1647
Author(s):  
Zeyneb Chaibeddra ◽  
Salah Akkal ◽  
Houria Ouled-Haddar ◽  
Artur M. S. Silva ◽  
Ammar Zellagui ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Health Benefit ◽  
Total Phenolic ◽  
Phytochemical Composition ◽  
Rat Paw Edema ◽  
Anti Inflammatory

Scrophularia tenuipes is an Algerian-Tunisian endemic species, which has not been studied yet. Ethyl acetate (EA) and n-butanol (Bu) fractions obtained from Scrophularia tenuipes were investigated for their health benefit properties, in particular with respect to in vivo/in vitro anti-inflammatory and antioxidant activities, as well as their potential to inhibit key enzymes with impact in diabetes (α-glucosidase and α-amylase). The fractions had a distinct phytochemical composition, of which EA was richer in total phenolic compounds (225 mg GAE/g) and mostly composed of the phenylethanoid acetyl martynoside. Compared to EA, Bu had higher amounts of total flavonoids, and according to the result obtained from UHPLC-DAD-ESI-MSn analysis, harpagoside (iridoid) was its major phytochemical. EA fraction was quite promising with regard to the in vivo (at 200 mg/kg, po) anti-inflammatory effect (62% and 52% for carrageenan-induced rat paw edema and xylene-induced ear edema tests, respectively), while Bu fraction exhibited a stronger antioxidant capacity in all tests (IC50 = 68 µg/mL, IC50 = 18 µg/mL, IC50 = 18 µg/mL and A0.50 = 43 µg/mL for DPPH●, ABTS•+, O2•− scavenging assays and cupric-reducing antioxidant capacity method, respectively). Both fractions also showed a strong effect against α-amylase enzyme (IC50 = 8 µg/mL and 10 µg/mL for EA and Bu fraction, respectively).

scholarly journals Cytotoxicity, Phytochemical, Antiparasitic Screening, and Antioxidant Activities of Mucuna pruriens (Fabaceae)

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1249 ◽  
Author(s):  
Mahboob Adekilekun Jimoh ◽  
Oladayo Amed Idris ◽  
Muhali Olaide Jimoh
Keyword(s):  
Gallic Acid ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Mucuna Pruriens ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Antioxidant Power ◽  
Pharmaceutical Industries

This study aimed at assessing the biological activities of Mucuna pruriens seeds using cytotoxicity, phytochemical, antiparasitic screening, and antioxidant assays. Mature fruits of M. pruriens were harvested from Fort Hare University’s Research Farm located in Alice, South Africa. The collected seeds were pulverized in a standard process and taken to the laboratory for crude extraction and further treatments. Cytotoxic, antimalarial, and trypanocidal effects of crude extracts obtained from ethanol and water were tested, while the total phenolic, proanthocyanidin, and flavonoid contents of the aqueous extracts as well as their pharmacological activities were determined in vitro using 2,2-diphenyl-1-picrylhydrazyl ethanol (DPPH), ferric reducing antioxidant power (FRAP), and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays. Although the extracts showed mild antiparasitic (antiplasmodial and trypanocidal) effects, results from the cytotoxic experiment revealed that M. pruriens is not toxic to human cervix adenocarcinoma (HeLa) cells when tested using 50 µg/mL of extracts. It was observed that the seeds were remarkably rich in phenol (3730.1 ± 15.52 mg gallic acid equivalent (GAE)/g) compared to flavonoids (63.03 ± 1.95 mg quercetin equivalent (QE)/g) and proanthocyanidin (18.92 ± 1.09 mg catechin equivalent (CE)/g). Also, the antioxidant activities of the extracts were comparable to those of the standard antioxidant drugs (rutin and gallic acid) used, in a concentration-dependent manner. There was a direct relationship between phenolic acid content and antioxidant effects. It is therefore suggested that M. pruriens seeds be incorporated into human diets as a supplement to promote healthy living. Pharmaceutical industries with a particular interest in natural phenolic acids should consider using seeds of M. pruriens as pharmaceutical precursors.

scholarly journals Anticholinergic, Antidiabetic and Antioxidant Activities of Ferula orientalis L. Determination of Its Polyphenol Contents by LC-HRMS

2021 ◽  
Vol 15 (6) ◽  
pp. 513-528
Author(s):  
Hatice Kızıltaş ◽  
Zeynebe Bingöl ◽  
Ahmet C. Gören ◽  
Saleh H. Alwasel ◽  
İlhami Gülçin
Keyword(s):  
Metal Ion ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Scavenging Activity ◽  
Ic50 Values ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

To evaluate the antioxidant activity of evaporated ethanolic extract of Ferula orientalis L. (EEFO) and lyophilized water extract of Ferula orientalis L. (WEFO) several in vitro antioxidant methods such as ABTS•+ scavenging activity, DPPH· scavenging activity, Fe3+reduction method, cupric ions (Cu2+) reduction capacity, and metal ion (Fe2+)-binding activities using ferrozine reagent were separately performed. Also, BHT, α-tocopherol and ascorbic acid were used as the standard antioxidant molecules. Moreover, some phenolic compounds that are responsible for antioxidant abilities of EEFO and WEFO were determined by LC-HRMS. EEFO and WEFO demonstrated effective antioxidant abilities when compared with the standards. EEFO demonstrated IC50 values of 1.946 µg/mL against acetylcholinesterase (AChE), 0.815 µg/mL against α-glycosidase, and 0.675 µg/mL against α-amylase.

Biological Activities and Dermal Penetration of Liposome-Containing Coprinus atramentraris Extract

2019 ◽  
Vol 819 ◽  
pp. 124-129
Author(s):  
Parapat Sobharaksha ◽  
Ratana Indranupakorn ◽  
Sonmkamol Manchan
Keyword(s):  
Particle Size ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Skin Permeation ◽  
Ethanolic Extract ◽  
Entrapment Efficiency ◽  
Strong Relationship ◽  
Antioxidant Property ◽  
Total Phenolic

The total phenolic content and antioxidant activities of five edible mushrooms (Pleurotus djamor, Agaricus bisporus, Hericlum erinaceus, Coprinus atramentraris and P. ostreatus) were evaluated. The Coprinus atramentraris ethanolic extract (CE) showed the promising antioxidant property and had a strong relationship with phenolic compound content. Liposome-containing CE formulations, were prepared with various lipid composition. The liposome formulation, L1, which composed of phospholipids and cholesterol at ratio of 6:4 gave the highest entrapment efficiency (24.03%), small particle size (143.03 nm) and favorable particle size distribution (0.311) with small values of zeta potential (-30.2 mV). L1 showed good anti-elastase property (IC50 0.029) and no cytotoxicity effect to the human skin fibroblasts and melanoma cells even at the highest concentration of 1 mg/mL compared to the unformulated extract. The in vitro skin permeation studies, using human cadaver skin and modified Franz-diffusion cells, showed that the L1 was able to considerably increase the rate of permeation of phenolic compounds in L1 compared to the CE solution. These results reflect the use of L1 as active agents in cosmeceuticals.

scholarly journals Influence of In Vitro Human Digestion Simulation on the Phenolics Contents and Biological Activities of the Aqueous Extracts from Turkish Cistus Species

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5322
Author(s):  
Yiğit İnan ◽  
Selin Akyüz ◽  
Inci Kurt-Celep ◽  
Engin Celep ◽  
Erdem Yesilada
Keyword(s):  
Oxidative Stress ◽  
Metabolic Diseases ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Plant Metabolites ◽  
Secondary Plant Metabolites ◽  
Phenolic Contents

Oxidative stress is one of the significant precursors of various metabolic diseases such as diabetes, Parkinson’s disease, cardiovascular diseases, cancer, etc. Various scientific reports have indicated that secondary plant metabolites play an important role in preventing oxidative stress and its harmful effects. In this respect, this study was planned to investigate the phenolic profile and antioxidant and antidiabetic potentials of the aqueous extracts from Turkish Cistus species by employing in vitro methods. In vitro digestion simulation procedure was applied to all extracts to estimate the bioavailability of their phenolic contents. Total phenolic, flavonoid, phenolic acid and proanthocyanidin contents were determined for all phases of digestion. In addition, changes in the quantity of the assigned marker flavonoids (tiliroside, hyperoside and quercitrin) were monitored by High-Performance Thin Layer Chromatography (HPTLC) analysis. The antioxidant activity potentials of the extracts were studied by various methods to reveal their detailed activity profiles. On the other hand, in vitro α-amylase and α-glucosidase enzymes and advanced-glycation end product (AGE) inhibitory activities of the extracts were determined to evaluate the antidiabetic potentials of extracts. The results showed that aqueous extracts obtained from the aerial parts of Turkish Cistus species have rich phenolic contents and potential antioxidant and antidiabetic activities; however, their bioactivity profiles and marker flavonoid concentrations might significantly be affected by human digestion. The results exhibited that total phenolic contents, antioxidant activities and diabetes-related enzyme inhibitions of the bioavailable samples were lower than non-digested samples in all extracts.

scholarly journals Quantification of total bioactive contents and evaluation of the antioxidant and antibacterial activities of crude extracts from Ephedra altissima Desf.

2021 ◽  
Vol 43 ◽  
pp. e52123
Author(s):  
Waffa Bouafia ◽  
Soumia Mouffouk ◽  
Hamada Haba
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Etoac Extract ◽  
Crude Extracts ◽  
Good Ability

The present work describes the in vitro biological activities of the crude extracts (petroleum ether, ethyl acetate and n-butanol) prepared from the species Ephedra altissima Desf. The estimation of total phenolic, flavonoid and tannin contents were carried out using the Folin-Ciocalteu, trichloroaluminum and acidified vanillin methods, respectively. The evaluation of the in vitro antioxidant activities were performed by three different methods namely: scavenging of the free radical ABTS, permanganate reducing antioxidant capacity, and potentiometric assay. In addition, the antibacterial activity was assessed by the agar disk diffusion assay against seven bacterial strains. The results of the phytochemical screening revealed the presence of several types of secondary metabolites. The EtOAc extract exhibited the highest content of phenols (125.62 ± 1.51 μg EGA mg-1 of extract). The greatest flavonoid and tannin contents were observed for n-BuOH extract (19.18 ± 0.39 µg EQ mg-1 of extract and 8.95 ± 1.70 μg EC mg-1 of extract, respectively). Moreover, the EtOAc extract revealed potent antioxidant activity in all the tested methods. Furthermore, the aqueous extract from the species E. altissima showed a good ability to reduce iron III to iron II with a value of 0.68 ± 0.3 moL eq L-1 in potentiometric assay. All the crude extracts (PE, EtOAc and n-BuOH) displayed inhibition of bacterial growth against at least three strains with values of MIC ranging from 3.125 to 50 µg mL-1. Therefore, these results suggest that Ephedra altissima could be used as an important source of natural bioactive compounds with antioxidant and antibacterial properties.

Phytochemical composition and antioxidant activity of Malus baccata (L.) Borkh. fruits

2020 ◽  
Vol 3 (1) ◽  
pp. 47
Author(s):  
Nadezhda Petkova ◽  
Manol Ognyanov ◽  
Blaga Inyutin ◽  
Petar Zhelev ◽  
Panteley Denev
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Titratable Acidity ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Frap Assay ◽  
Phytochemical Composition ◽  
Malus Baccata

Crab apple (Malus baccata (L.) Borkh.) was mainly distributed in Europe as an ornamental plant, but the nutritional properties of its edible fruits were not fully revealed. The aim of the current study was to characterize the phytochemical composition of ripen carb apple fruits and to evaluate their nutritional and antioxidant potentials. The fruits were assayed for moisture and ash content, proteins, lipids, carbohydrates, titratable acidity (TA), pH, total phenolic compounds and natural pigments. Among the analyzed carbohydrates cellulose was found in the highest content (6% dw), followed by sugars (sucrose, glucose and fructose) and 1.8 % dw uronic acids. The total chlorophylls and carotenoids contents in their fruits were 6.51 and 4.80 μg/g fw, respectively. Total monomeric anthocyanins were not detected. The highest content of total phenolic compounds (2.67 mg GAE/g fw) was found in 95 % ethanol extract from fruits, while the total flavonoids were relatively low – 0.1 mg QE/g fw. DPPH assay (17.27 mM TE/g fw) and FRAP assay (14.34 mM TE/g fw) demonstrated in vitro antioxidant activities of crabapple. Malus baccata fruits were evaluated as a rich source of dietary fibers and phenolic compounds with significant antioxidant potential that could be used in human nutrition.

Chemical Constituents Isolated from Rhododendron ungernii with Antioxidant Profile

2019 ◽  
Vol 9 (3) ◽  
pp. 238-243 ◽  
Author(s):  
Emine Dede ◽  
Nusret Genc ◽  
Mahfuz Elmastas ◽  
Huseyin Aksit ◽  
Ramazan Erenler
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Isolation And Identification ◽  
Reduced Pressure ◽  
Scavenging Effect ◽  
Pharmaceutical Industries ◽  
Dpph Scavenging

Background: Plant in Rhododendron genus that contains phenolic compounds has been used in traditional medicine and revealed considerable biological activities. Objective: Isolation and identification of antioxidant natural products from Rhododendron ungernii. Methods: Rhododendron ungernii Trautv. flowers were collected and dried in shade. The dried flowers were extracted with methanol for 3 days. The solvent was removed by reduced pressure to yield the extract which was subjected to column chromatography (Sephadex LH-20, C18 reversed phase column) to isolate catechin-7-O-glucoside (1), quercetin-3-O-β-galactoside (2), quercetin-3-O- β-xyloside (3), farrerol (4), myricetin (5), and quercetin (6). The structures of isolated compounds were elucidated by spectroscopic methods such as 1D-NMR, 2D-NMR, and LC-TOF/MS. DPPH scavenging effect, ABTS+ scavenging activity, and reducing power (FRAP) were performed for antioxidant assays of isolated natural compounds. Results: Isolated flavonoids displayed the outstanding antioxidant activities. Catechin-7-O-glucoside (1) and quercetin-3-O-β-galactoside (2) (IC50, 3.66 µg/mL) had the most DPPH• scavenging effect among the compounds. The highest ABTS•+ scavenging activity (IC50, 1.41 µg/mL) and reducing power effect (6.05 mmol TE/g comp) were observed for myricetin (5). Conclusion: R. ungernii extract and isolated compounds could be a promising antioxidant for food and pharmaceutical industries.

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