scholarly journals IN VITRO ANTI-ARTHRITIC ACTIVITY OF DIFFERENT EXTRACTS OF CASCABELA THEVETIA (THEVETIA PERUVIANA ) LEAVES

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Subba Rao Chamakuri ◽  
Dasari Priyanka ◽  
Syed Ahad Hussain ◽  
Rohama Ayyub ◽  
Avula Latha ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Protein Denaturation ◽  
Chemical Constituents ◽  
Chloroform Extract ◽  
In Vitro Studies ◽  
The Other ◽  
Reference Standard ◽  
Thevetia Peruviana ◽  
Dose Dependent

Cascabela thevetia (Thevetia peruviana) giant milkweed, is known for its pharmacological importance for centuries. The chemical constituents of Thevetia peruviana leaves are amyrin, amyrin acetate, ?-sitosterol, urosolic acid, cardenolides, calotropin. The anti-arthritic activity of Thevetia peruviana leaves in comparison with Diclofenac was evaluated through in-vitro studies. In-vitro studies revealed anti-arthritic effects (p< 0.05) of crude extracts of aqueous, methanolic, ethanolic and chloroform of Thevetia peruviana leaves on bovine serum albumin as evident for % inhibition protein denaturation. Different extracts of Thevetia peruviana leaves were found to possess in-vitro antiarthritic activity against BSA, using diclofenac as the reference standard which are Dose-dependent (100µg/ml, 250µg/ml, 500µg/ml). Chloroform extract has shown better activity comparing the other extracts.

Influence of SIN-1 on Platelet Ca2+ Handling in Patients with Suspected Coronary Artery Disease: Ex Vivo and In Vitro Studies

2000 ◽  
Vol 83 (05) ◽  
pp. 752-758 ◽  
Author(s):  
Claude Le Feuvre ◽  
Annie Brunet ◽  
Thuc Do Pham ◽  
Jean-Philippe Metzger ◽  
André Vacheron ◽  
...  
Keyword(s):  
Superoxide Anion ◽  
Vascular Tone ◽  
Ex Vivo ◽  
Suspected Coronary Artery Disease ◽  
In Vitro Studies ◽  
H2o2 Formation ◽  
Artery Disease ◽  
Dose Dependent

SummaryThe 3-morpholinosydnonimine (SIN-1) generates both nitric oxide (NO) and superoxide anion (O2−). It elicits dose-dependent vasodilation in vivo, in spite of the opposite effects of its breakdown products on vascular tone and platelet aggregation.This study was designed to investigate the influence of intravenous SIN-1 injection on platelet Ca2+ handling in patients undergoing coronary angiography. SIN-1 administration reduced cytosolic [Ca2+] in unstimulated platelets by decreasing Ca2+ influx. It attenuated Ca2+ mobilization from internal stores evoked by thrombin or thapsigargin. In vitro studies were used as an approach to investigate how simultaneous productions of NO and O2− from SIN-1 modify thrombin- or thapsigargin-induced platelet Ca2+ mobilization. Superoxide dismutase, the O2− scavenger, enhanced the capacity of SIN-1 to inhibit Ca2+ mobilization but catalase had no effect.This suggests that the effects of SIN-1 on platelet Ca2+ handling resemble those of NO, but are modulated by simultaneous O2− release, independently of H2O2 formation.

scholarly journals Delaying meiotic resumption during transportation of bovine cumulus–oocyte complexes: effects on development, apoptosis and caspases activity of in vitro-produced embryos

Zygote ◽  
2017 ◽  
Vol 25 (6) ◽  
pp. 740-750 ◽  
Author(s):  
Priscila Chediek Dall'Acqua ◽  
Beatriz Caetano da Silva Leão ◽  
Nathália Alves de Souza Rocha-Frigoni ◽  
Fernanda Patrícia Gottardi ◽  
Gisele Zoccal Mingoti
Keyword(s):  
Bovine Serum Albumin ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
The Other ◽  
Developmental Competence ◽  
Bovine Oocyte ◽  
Control Groups ◽  
Meiotic Inhibitors

SummaryThis study examined the effects of meiosis inhibition during bovine oocyte transportation on developmental competence and quality of produced embryos. The transportation medium was supplemented with: 100 μM butyrolactone I (BL), 500 μM IBMX + 100 μM forskolin (mSPOM), 100 μM milrinone (MR) or follicular fluid (bFF), and was carried out in a portable incubator for 6 h. Next, oocytes were in vitro matured (IVM) for 18 h, without the meiotic inhibitors, with the exception of mSPOM group, in which was added 20 μM cilostamide. The three control groups were IVM with 10% fetal calf serum (FCS) (Control Lab FCS) or 0.6% bovine serum albumin (BSA) (Control Lab BSA) in a CO2 in air incubator or in the portable incubator with 0.6% BSA (Control Transp BSA). Higher cleavage rates (P < 0.05) were obtained in the Control Lab FCS group (84.5 ± 5.3%) compared with the other groups (59.6 ± 3.4% to 70.9 ± 2.3%). Embryonic development was higher (P < 0.05) in the Control Lab FCS group (39.8 ± 4.7%) than in the Control Transp BSA (22.7 ± 3.4%) and MR (21.6 ± 2.3%) groups. However, they were similar (P > 0.05) to the other groups (23.6 ± 3.3% to 28.8 ± 2.7%). The total number of blastomeres was higher (P < 0.05) in the Control Lab FCS group (85.2 ± 5.6) than in Control Lab BSA (53.6 ± 2.9), Control Transp BSA (55.5 ± 4.4), BL (58.2 ± 3.0), mSPOM (57.9 ± 4.9) and MR (59.2 ± 3.9), but all these treatments did not differ (P > 0.05) from bFF (67.7 ± 4.2). No differences (P > 0.05) were found in apoptosis by the activity of caspases (139.0 ± 3.2 to 152.4 ± 6.5, expressed in fluorescence intensity) as well as the percentage of TUNEL-positive cells (12.3 ± 2.0% to 15.7 ± 1.7%). In conclusion, the transportation of oocytes over 6 h with BL, mSPOM or bFF enabled the acquisition of developmental competence at similar rates to the Control Lab FCS group.

THE ROLE OF ADSORPTION IN CONTROLLING THE RATE OF REACTION OF CHLORAMBUCIL WITH PROTEIN

1963 ◽  
Vol 41 (4) ◽  
pp. 931-939 ◽  
Author(s):  
J. H. Linford
Keyword(s):  
Molecular Weight ◽  
Carboxylic Acid ◽  
Bovine Serum Albumin ◽  
Inverse Relationship ◽  
Reaction Rate ◽  
The Other ◽  
Alkylation Reaction ◽  
Rate Of Reaction

Two proteins, haemoglobin and bovine serum albumin, have been studied with respect to their rates of alkylation by chlorambucil in vitro at 37 °C and pH 8.4. The proteins are of nearly the same molecular weight and free carboxylic acid content, but the alkylation reaction is 30 times faster with haemoglobin. On the other hand, the adsorption of chlorambucil by albumin is 20 times greater than that exhibited by haemoglobin. This inverse relationship between extent of adsorption and reaction rate suggests that adsorption protects the chlorambucil from activation in the solvent.

scholarly journals Anthelmintic and Cytotoxic Activities of Two Medicinal Plants: Polygonum viscosum and Aphanamixis polystachya Growing in Bangladesh

2014 ◽  
Vol 6 (2) ◽  
pp. 339-345 ◽  
Author(s):  
M. N. Amin ◽  
M. S. Majumder ◽  
M. M. R. Moghal ◽  
S. Banik ◽  
A. Kar ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Anthelmintic Activity ◽  
Cytotoxic Activities ◽  
Dependent Manner ◽  
Reference Standard ◽  
Cytotoxic Potential ◽  
Lc50 Value ◽  
Vincristine Sulphate ◽  
Dose Dependent

The present study was designed to investigate in vitro anthelmintic and cytotoxic activities of crude methanolic extract of two plants(Polygonum viscosum and Aphanamixis polystachya) grown in Bangladesh. Evaluation of cytotoxic activity was done using the brine shrimp lethality bioassay. The crude methanolic extract of Polygonum viscosum showed significant cytotoxic potential (LC50 value of 6.34 ?g/ml) among all the fractions comparing with that of standard vincristine sulphate (0.825 ?g/ml). Besides, the LC50 values of crude methanolic extract, pet ether and chloroform extracts of Aphanamixis polystachya showed good cytotoxic activities 11, 10.36, and 16.45 µg/ml, respectively. The other study was undertaken to evaluate anthelmintic activity (using Pheretima posthuma model) where piperazine was used as reference standard. The crude methanolic extract of Polygonum viscosum leaves produced a significant anthelmintic activity in dose dependent manner and the activity of crude extract was comparable with that of standard drugs. Besides, the Aphanamixis polystachya extract revealed moderate anthelmintic activity. Here, the anova testing was done with the P < 0.05. Further studies are suggested to determine the active compounds responsible for the anthelmintic and cytotoxic activities of these two plant extracts.   Keywords: Anthelmintic; Cytotoxic; Medicinal plant; Aphanamixis polystachya; Polygonum viscosu.  © 2014 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.   doi: http://dx.doi.org/10.3329/jsr.v6i2.17299 J. Sci. Res. 6 (2), 339-345 (2014) 

scholarly journals Comparative extraction, phytochemical screening and in vitro biological activities of Eclipta prostrata extract

2020 ◽  
Vol 10 (4-s) ◽  
pp. 148-152
Author(s):  
Zafar Alam Khan ◽  
Salaj Khare ◽  
B.K. Dubey
Keyword(s):  
Therapeutic Potential ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Antidiabetic Activity ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Eclipta Prostrata ◽  
Promising Source ◽  
Dose Dependent

Medicinal plants possess therapeutic potential and are used to treat various diseases around the world. Eclipta prostrate (L.) is a medicinal herb that has extensive application in the native medicinal system. In any therapeutic activity chemical constituents play an important role. Eclipta prostrata has been investigated in this study for its antioxidant, antimicrobial and antidiabetic activity in vitro. The well-known research protocol available in the literature established qualitative analysis of the different phytochemical constituents and quantitative analysis of total phenol and flavonoids. The hydroalcoholic extracts of the leaves and seeds of Eclipta prostrata exhibited significant and dose-dependent antioxidant activity including ability to donate electron. To analyze the antimicrobial activity, Leaves hydroalcoholic extracts and Eclipta prostrate seeds were tested against two selected strains using a well-diffusion method and showing significant inhibitory action against all the strain tested. In addition, the dose-dependent α-amylase inhibitory activity with an IC50 value of acarbose, leaves, and seed extract was found to be 364.89μg/ml and 438.43μg/ml, respectively, indicating that Eclipta prostrate is a promising source as an herbal medicine. Keywords: Eclipta prostrate, Phytochemical Analysis, Antioxidant, Antimicrobial, Antidiabetic Activity.

scholarly journals Quantitative analysis of chemical constituents in medicinal plant coleus aromaticus extracts

2018 ◽  
Vol 6 (3) ◽  
pp. 1002 ◽  
Author(s):  
Santhosh Kumar N. ◽  
Aliya Nusrath ◽  
Dinesha Ramadas
Keyword(s):  
Medicinal Plant ◽  
Protein Concentration ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
Chloroform Extract ◽  
Alcoholic Extract ◽  
Protein Extract ◽  
Total Sugars ◽  
Coleus Aromaticus

Background: Indian medicinal plants have great potential towards curing many diseases. Medicinal plant Coleus aromaticus is known for its wide medical applications. The main objectives of the study undertaken were to analyze the phytochemicals and compare the concentration present in the dialyzed Coleus aromaticus protein extract with various solvents.Methods: Various extracts of the medicinal plant Coleus aromaticus leaves such as dialyzed Coleus aromaticus protein, hydro alcoholic extract, ethanol extract and chloroform extract were prepared and analyzed for various phytochemical concentrations such as Total phenols (Folin-Ciocalteu method), Flavonoids (Aluminum chloride method), total sugars (Dubois method) and Proteins (Bradford’s method). Standard graph for each component was plotted.Results: The protein concentration in dialyzed Coleus aromaticus protein extract is considerably higher (5.8μg/10μl) than hydro alcoholic extract (1.6μg/10μl), ethanol extract (5.2μg/10μl) and chloroform extract (2.8μg/10μl). The other phytochemicals like total phenol, flavonoids and total sugars were low in concentration in the dialyzed Coleus aromaticus protein extract compared to hydro alcoholic extract, ethanol extract and chloroform extract.Conclusions: In this study, the protein concentration in dialyzed Coleus aromaticus protein extract is considerably higher than the same in other solvent extracts. This is the basis for further studies to unfold the antioxidant activity of Coleus aromaticus protein in vitro.

scholarly journals Antifilarial Screening and Oxidative Role of Isolated Fraction from Aegle Marmelos Corr. Leaves Extract

2021 ◽  
Vol 18 (2) ◽  
pp. 395-401
Author(s):  
Sahare K N
Keyword(s):  
Control Level ◽  
Chloroform Extract ◽  
Aegle Marmelos ◽  
Setaria Cervi ◽  
Parasite Motility ◽  
Antifilarial Activity ◽  
Dose Dependent ◽  
Mtt Assays

In the present work antifilarial active fraction was isolated from the leaves Chloroform extract of Aegle marmelos Corr. evaluated in vitro for antifilarial activity and studied the possible oxidative role against Setaria cervi parasite. Antifilarial study was carried out with isolated fractions by worm motility and MTT assays. Complete parasite motility inhibition was observed at 0.002 to 0.08 mg/mL in motility assay and in MTT assay plant fraction gave > 50% reduction 58.9, 74.6 and 97.2% at concentrations 0.02, 0.04 and 0.08 mg/mL at 10, 6 and 2 hours incubation period respectively (p< 0.05). Inhibitory concentration (IC50) was found to be 0.015 mg/mL. Oxidative parameters levels for MDA, Carbonyl content and Nitric oxide were identified as antifilarial activity achieved. The level of oxidative parameters was calculated in dose dependent manners as compared to the control level. The antifilarial activity of isolated fraction is associated with the oxidative mechanism in this study.

scholarly journals Chemical Constituents of Equisetum Debile and their Cytotoxic Activity

2008 ◽  
Vol 3 (11) ◽  
pp. 1934578X0800301
Author(s):  
Tran Huy Thai ◽  
Nguyen Quang Hung ◽  
Chau Van Minh ◽  
Nguyen Xuan Cuong ◽  
Pham Hai Yen ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cancer ◽  
Chemical Constituents ◽  
In Vitro Assay ◽  
The Other ◽  
Human Cancer Cell ◽  
Vitro Assay ◽  
Human Cancer Cell Lines ◽  
Aerial Parts

A new phenyl glycoside, equisetumoside D (1), was isolated from the aerial parts of Equisetum debile, along with equisetumoside B (2), dehydrovomifoliol (3), corchoionoside C (4), (-)-isolariciresinol-3a-O-β-D-glucopyranoside (5), and kaempferol 3-O-sophoroside-7-O-β-D-glucopyranoside (6). Their structures were elucidated by NMR spectroscopic and MS experiments. Compound 1 was found to be cytotoxic against both tested human cancer cell lines, hepatocellular carcinoma (Hep-G2, IC50: 1.12 μg/mL) and rhabdosarcoma (RD, IC50: 0.25 μg/mL), while the other compounds showed no activity against these cell lines by in vitro assay.

Factors Affecting β2-Microglobulin Plasma Concentration during Hemodialysis

1989 ◽  
Vol 12 (8) ◽  
pp. 509-514 ◽  
Author(s):  
A. Martin-Malo ◽  
J. Mallol ◽  
D. Castillo ◽  
V. Barrio ◽  
L.G. Burdiel ◽  
...  
Keyword(s):  
Plasma Concentration ◽  
Mass Transport ◽  
Adsorption Capacity ◽  
Plasma Concentrations ◽  
Extracellular Volume ◽  
In Vitro Studies ◽  
The Other ◽  
High Permeability ◽  
Factors Affecting

We studied ten patients on hemodialysis (HD) treated in sequence with cuprophan (CU), ethylenevinylalcohol (EVAL), polyacrylonitrile (A-69) and polysulphone (PSP) membrane dialyzers. ß2-microglobulin (ß2m) was measured by radioimmunoassay in plasma and dialysate samples. Plasma concentrations were corrected for changes in extracellular volume (ECV). We also studied adsorption in vitro by incubating the above membranes with I-125-labelled ß2m. There were no changes in ß2m plasma concentration after HD with CU dialyzers, but a significant decrease was observed with the other membranes tested. Filtration of ß2m across the dialyzer was absent with CU and minimal with EVAL. However, large amounts were recovered from dialysate with the high-permeability dialyzers, AN-69 and PSP. In vitro studies showed that maximal adsorption capacity was obtained with AN-69 (73%) compared to 9% with CU, 4% with EVAL and 7% with PSP. In summary, ß2m clearance with PSP is achieved through greater removal of this protein by mass transport across the membrane. The mechanism by which ß2m is removed from blood during AN-69 dialysis seems to include both adsorption to and filtration by the membrane itself.

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