scholarly journals THE FUNCTIONAL STATUS OF NEUTROPHILS IN PATIENTS WITH CHRONIC RECURRENT INFECTIONS OF VARIOUS ETIOLOGY

2016 ◽  
pp. 48-53
Author(s):  
N. V. Gusakova ◽  
I. A. Novikova
Keyword(s):  
Functional Status ◽  
Bactericidal Activity ◽  
Acute Stage ◽  
Net Generation ◽  
No Production ◽  
Recurrent Infections ◽  
Healthy Individuals ◽  
Herpetic Infection ◽  
Recurrent Furunculosis

Objective: complex assessment of the functional status of neutrophils in patients with chronic recurrent furunculosis (CRF) and recurrent herpetic infection (RHI). Material and methods. We have studied the functional status of neutrophils: ROS-, NO-production and NET-generation, apoptosis, phagocytosis, and bactericidal ability of 103 patients with CRF and 238 patients with RHI examined during remission and exacerbation stages. Conclusion. The patients examined during remission showed the same type of the functional status changes of neutrophils in comparison with healthy individuals. The exception was the parameters of the bactericidal activity of neutrophils decreased in the patients with CRF. During the acute stage we marked the maximum changes of netosis and apoptosis. We have defined the possibility of using these data for more precise differentiation of exacerbation and remission in patients with CRF and RHI.

scholarly journals Chikungunya Manifestations and Viremia in Patients Who Presented to the Fever Clinic at Bangkok Hospital for Tropical Diseases during the 2019 Outbreak in Thailand

2021 ◽  
Vol 6 (1) ◽  
pp. 12
Author(s):  
Hisham A Imad ◽  
Juthamas Phadungsombat ◽  
Emi E Nakayama ◽  
Sajikapon Kludkleeb ◽  
Wasin Matsee ◽  
...  
Keyword(s):  
Chikungunya Virus ◽  
Clinical Manifestations ◽  
Clinical Findings ◽  
Acute Stage ◽  
Tropical Diseases ◽  
Healthy Individuals ◽  
Rt Pcr ◽  
Viral Loads ◽  
The Family ◽  
Systemic Symptoms

Chikungunya virus is an Alphavirus belonging to the family Togaviridae that is transmitted to humans by an infected Aedes mosquito. Patients develop fever, inflammatory arthritis, and rash during the acute stage of infection. Although the illness is self-limiting, atypical and severe cases are not uncommon, and 60% may develop chronic symptoms that persist for months or even for longer durations. Having a distinct periodical epidemiologic outbreak pattern, chikungunya virus reappeared in Thailand in December 2018. Here, we describe a cohort of acute chikungunya patients who had presented to the Bangkok Hospital for Tropical Diseases during October 2019. Infection was detected by a novel antigen kit and subsequently confirmed by real-time RT-PCR using serum collected at presentation to the Fever Clinic. Other possible acute febrile illnesses such as influenza, dengue, and malaria were excluded. We explored the sequence of clinical manifestations at presentation during the acute phase and associated the viral load with the clinical findings. Most of the patients were healthy individuals in their forties. Fever and arthralgia were the predominant clinical manifestations found in this patient cohort, with a small proportion of patients with systemic symptoms. Higher viral loads were associated with arthralgia, and arthralgia with the involvement of the large joints was more common in female patients.

scholarly journals Role of Antilipopolysaccharide Antibodies in Serum Bactericidal Activity against Salmonella enterica Serovar Typhimurium in Healthy Adults and Children in the United States

2013 ◽  
Vol 20 (10) ◽  
pp. 1491-1498 ◽  
Author(s):  
Estela Trebicka ◽  
Susan Jacob ◽  
Waheed Pirzai ◽  
Bryan P. Hurley ◽  
Bobby J. Cherayil
Keyword(s):  
United States ◽  
Bactericidal Activity ◽  
Salmonella Enterica ◽  
The United States ◽  
Healthy Children ◽  
Healthy Individuals ◽  
Serum Samples ◽  
Content Type ◽  
Serovar Typhimurium

ABSTRACTRecent observations from Africa have rekindled interest in the role of serum bactericidal antibodies in protecting against systemic infection withSalmonella entericaserovar Typhimurium. To determine whether the findings are applicable to other populations, we analyzed serum samples collected from healthy individuals in the United States. We found that all but 1 of the 49 adult samples tested had robust bactericidal activity againstS. Typhimurium in a standardin vitroassay. The activity was dependent on complement and could be reproduced by immunoglobulin G (IgG) purified from the sera. The bactericidal activity was inhibited by competition with soluble lipopolysaccharide (LPS) fromS. Typhimurium but not fromEscherichia coli, consistent with recognition of a determinant in the O-antigen polysaccharide. Sera from healthy children aged 10 to 48 months also had bactericidal activity, although it was significantly less than in the adults, correlating with lower levels of LPS-specific IgM and IgG. The lone sample in our collection that lacked bactericidal activity was able to inhibit killing ofS. Typhimurium by the other sera. The inhibition correlated with the presence of an LPS-specific IgM and was associated with decreased complement deposition on the bacterial surface. Our results indicate that healthy individuals can have circulating antibodies to LPS that either mediate or inhibit killing ofS. Typhimurium. The findings contrast with the observations from Africa, which linked bactericidal activity to antibodies against anS. Typhimurium outer membrane protein and correlated the presence of inhibitory anti-LPS antibodies with human immunodeficiency virus infection.

scholarly journals Effect of Human Immunodeficiency Virus Infection on Plasma Bactericidal Activity against Salmonella enterica Serovar Typhimurium

2014 ◽  
Vol 21 (10) ◽  
pp. 1437-1442 ◽  
Author(s):  
Estela Trebicka ◽  
Nanda Kumar N. Shanmugam ◽  
Anastassia Mikhailova ◽  
Galit Alter ◽  
Bobby J. Cherayil
Keyword(s):  
Human Immunodeficiency Virus ◽  
Bactericidal Activity ◽  
Salmonella Enterica ◽  
Hiv Positive ◽  
Healthy Controls ◽  
Elite Controllers ◽  
Healthy Individuals ◽  
Immunodeficiency Virus ◽  
Serovar Typhimurium ◽  
Specific Igg

ABSTRACTIndividuals with human immunodeficiency virus (HIV) infection have increased susceptibility to invasive disease caused bySalmonella entericaserovar Typhimurium. Studies from Africa have suggested that this susceptibility is related in part to the development of a high level of lipopolysaccharide (LPS)-specific IgG that is able to inhibit the killing ofS. Typhimurium by bactericidal antibodies in healthy individuals. To explore this issue further, we examined the bactericidal activity againstS. Typhimurium using serum and plasma samples from healthy controls and various clinical subgroups of HIV-infected adults in the United States. We found that the bactericidal activity in the samples from HIV-positive elite controllers was comparable to that from healthy individuals, whereas it was significantly reduced in HIV-positive viremic controllers and untreated chronic progressors. As demonstrated previously for healthy controls, the bactericidal activity of the plasma from the elite controllers was inhibited by preincubation withS. Typhimurium LPS, suggesting that it was mediated by anti-LPS antibodies.S. Typhimurium LPS-specific IgG was significantly reduced in all subgroups of HIV-infected individuals. Interestingly, and in contrast to the healthy controls, plasma from all HIV-positive subgroups inhibitedin vitrokilling ofS. Typhimurium by plasma from a healthy individual. Our results, together with the findings from Africa, suggest that multiple mechanisms may be involved in the HIV-induced dysregulation of humoral immunity toS. Typhimurium.

Functional status in patients with chronic fatigue syndrome, other fatiguing illnesses, and healthy individuals

1996 ◽  
Vol 101 (4) ◽  
pp. 364-370 ◽  
Author(s):  
Dedra Buchwald ◽  
Tsilke Pearlman ◽  
Jovine Umali ◽  
Karen Schmaling ◽  
Wayne Katon
Keyword(s):  
Chronic Fatigue Syndrome ◽  
Functional Status ◽  
Chronic Fatigue ◽  
Healthy Individuals ◽  
Fatigue Syndrome

scholarly journals Bactericidal Activity of Human Eosinophilic Granulocytes against Escherichia coli

2001 ◽  
Vol 69 (6) ◽  
pp. 3591-3596 ◽  
Author(s):  
Terese Persson ◽  
Pia Andersson ◽  
Mikael Bodelsson ◽  
Martin Laurell ◽  
Johan Malm ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bactericidal Activity ◽  
Bacterial Invasion ◽  
No Production ◽  
Minor Importance ◽  
Experimental Conditions ◽  
Bacterial Killing ◽  
Gram Negative ◽  
E Coli ◽  
Diphenylene Iodonium

ABSTRACT Eosinophils participate in allergic inflammation and may have roles in the body's defense against helminthic infestation. Even under noninflammatory conditions, eosinophils are present in the mucosa of the large intestine, where large numbers of gram-negative bacteria reside. Therefore, roles for eosinophils in host defenses against bacterial invasion are possible. In a system for bacterial viable counts, the bactericidal activity of eosinophils and the contribution of different cellular antibacterial systems against Escherichia coli were investigated. Eosinophils showed a rapid and efficient killing of E. coli under aerobic conditions, whereas under anaerobic conditions bacterial killing decreased dramatically. In addition, diphenylene iodonium chloride (DPI), an inhibitor of the NADPH oxidase and thereby of superoxide production, also significantly inhibited bacterial killing. The inhibitor of nitric oxide (NO) productionl-N 5-(1-iminoethyl)-ornithine dihydrochloride did not affect the killing efficiency, suggesting that NO or derivatives thereof are of minor importance under the experimental conditions used. To investigate the involvement of superoxide and eosinophil peroxidase (EPO) in bacterial killing, EPO was blocked by azide. The rate of E. coli killing decreased significantly in the presence of azide, whereas addition of DPI did not further decrease the killing, suggesting that superoxide acts in conjunction with EPO. Bactericidal activity was seen in eosinophil extracts containing granule proteins, indicating that oxygen-independent killing may be of importance as well. The findings suggest that eosinophils can participate in host defense against gram-negative bacterial invasion and that oxygen-dependent killing, i.e., superoxide acting in conjunction with EPO, may be the most important bactericidal effector function of these cells.

scholarly journals Chikungunya Manifestations and Viremia in Patients Who Presented to the Fever Clinic at Bangkok Hospital for Tropical Diseases During the 2019 Outbreak in Thailand

2020 ◽  
Author(s):  
Hisham A Imad ◽  
Juthamas Phadungsombat ◽  
Emi E Nakayama ◽  
Sajikapon Kludkleeb ◽  
Wasin Matsee ◽  
...  
Keyword(s):  
Chikungunya Virus ◽  
Clinical Manifestations ◽  
Clinical Findings ◽  
Acute Stage ◽  
Tropical Diseases ◽  
Healthy Individuals ◽  
Rt Pcr ◽  
Viral Loads ◽  
The Family ◽  
Systemic Symptoms

Chikungunya virus is an Alphavirus belonging to the family Togaviridae that is transmitted to humans by an infected Aedes mosquito. Patients develop fever, inflammatory arthritis, and rash during the acute stage of infection. Although the illness is self-limiting, atypical and severe cases are not uncommon, and 60% may develop chronic symptoms that persist for months or even for longer durations. Having a distinct periodical epidemiologic outbreak pattern, chikungunya virus reappeared in Thailand in December 2018. Here, we describe a cohort of acute chikungunya patients who had presented to the Bangkok Hospital for Tropical Diseases during October 2019. Infection was confirmed by real-time RT-PCR using serum collected at presentation to the Fever Clinic. Other possible acute febrile illnesses such as influenza, dengue, and malaria were excluded. We explored the sequence of clinical manifestations at presentation during the acute phase and associated the viral load with the clinical findings. Most of the patients were healthy individuals in their forties. Fever and arthralgia were the predominant clinical manifestations found in this patient cohort, with a small proportion of patients with systemic symptoms. Higher viral loads were associated with arthralgia, and arthralgia with the involvement of the large joints was more common in female patients

scholarly journals MANAGING THE EARLY PHASE OF REHABILITATION AFTER ISCHEMIC CEREBROVASCULAR ACCIDENT

2017 ◽  
Vol 9 (6) ◽  
pp. 194
Author(s):  
Eqrem Gara ◽  
Bekim Haxhiu ◽  
Zheralldin Durguti ◽  
Ardiana Murtezani
Keyword(s):  
Functional Status ◽  
Cerebrovascular Accident ◽  
Recurrent Stroke ◽  
Acute Stage ◽  
Neurological Deficits ◽  
Medical Emergency ◽  
Statistical Parameters ◽  
Chi Square ◽  
Permanent Disability ◽  
Rehabilitation Intervention

Objective: Ischemic cerebrovascular accident (ICA) is characterised by the rapid deterioration of brain function due to vascularization disorders. The ischemic cerebrovascular accident may arise as a consequence of ischemia or hemhorrage in brain tissue, but the optimal treatment approach is unclear. In this study, we examined ICA rehabilitation goals, implementation of rehabilitation plans, management of sensomotor deficits and functional status, improvement of independence, prevention and treatment of complications, functional status monitoring, and planning recommendations, as well as education of ICA patients and their families.Methods: This study considered 69 ICA patients who were classified as suitable for rehabilitation intervention among a total of 231 patients who were evaluated for a diagnosis of ICA following hospitalization at the Clinic of Neurology, University Clinical Center of Kosovo (UCCK) in Prishtina. From the statistical parameters, we used the structure index, whereas testing results were evaluated using the Chi-square test with significance established at p<0.05.Results: For the 69 ICA patients, most ICA incidents occurred in patients who were older than 40 y-old. Mobility decreased from 42.9% to 8.6% when rehabilitation began at release instead of admission. Meanwhile, the ability to perform daily activities decreased from 48.6% to 11.4% when rehabilitation began after release as opposed to immediately after stabilization. Transfers and balance showed similar decreases (48.6% to 11.4% and 48.6% to 11.4%, respectively Chi-test=59.7, p<0.001.). The rehabilitation of patients in the early acute stage after ICA should begin as soon as possible after the diagnosis is made and the patient's condition is stable. During rehabilitation intervention, priority should be given to preventing complications and recurrent stroke, as well as enhancing patient mobility and improving patient morale.Conclusion: ICA is a medical emergency that can cause permanent neurological lesions and other complications that may be fatal or associated with permanent disability. The most affected age group is individuals older than 40 y old. Neurological deficits can cause motor, sensory, functional and emotional disability in ICA patients. Rehabilitation after ICA should begin immediately after the patient has stabilized to minimze functional losses.

scholarly journals Sodium tetraphenylborate displays selective bactericidal activity against N. meningitidis and N. gonorrhoeae and is effective at reducing bacterial infection load.

2020 ◽  
Author(s):  
Eve Bernet ◽  
Marthe Lebughe ◽  
Antony T. Vincent ◽  
Mohammad Mehdi Haghdoost ◽  
Golara Golbaghi ◽  
...  
Keyword(s):  
Bactericidal Activity ◽  
Cell Envelope ◽  
Biological Activities ◽  
Lower Sensitivity ◽  
Healthy Individuals ◽  
Sodium Tetraphenylborate ◽  
Normal Flora ◽  
Boron Uptake ◽  
Icp Ms ◽  
Higher Doses

Neisseria meningitidis and Neisseria gonorrhoeae, two highly related species that may have emerged from a common commensal ancestor, constitute major human threats. Vaccines are available to prevent N. meningitidis infection, whereas for N. gonorrhoeae, there are only a limited number of antibiotics available. Unfortunately, some strains of these species are rapidly evolving and capable of escaping human interventions. Thus, it is now urgent to develop new avenues to fight these bacteria. This study reports that a boron-based salt, sodium tetraphenylborate (NaBPh4), displays high bactericidal activity and remarkable specificity against N. meningitidis and N. gonorrhoeae. Other closely related commensal species such as N. lactamica, found in the normal flora of healthy individuals were found to be less affected even at 5-fold higher doses of NaBPh4. This specificity was further observed where much lower sensitivity was found for the more distant Neisseriaceae (such as N. elongata or Kingella oralis) and completely unrelated species. A significant boron uptake by N. meningitidis cells was observed after incubation with 5 μM of NaBPh4, as measured by ICP-MS, suggesting that this drug candidate's target(s) could be located intracellularly or within the cell envelope. Furthermore, mutants with a slightly decreased susceptibility displayed an alteration in genes coding for cell-envelope elements, which reduced their virulence in an animal model of infection. Finally, a single dose of NaBPh4 resulted in a significant reduction in bacterial burden in a mouse model of N. meningitidis bacteremia. Although numerous boron-containing species were previously reported for their complex biological activities, the observation of this narrow selectivity is unprecedented and of potential importance from a therapeutic standpoint.

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