Soil Biogeochemical Response to Drought Conditions in the Biosphere 2 Rainforest
<p>The direct measurement of soil gases provides insight into the biogeochemical processes responsible for micro- and macro-nutrient cycling, respiration, signaling, and environmental responses.&#160; The concentrations and isotopic signatures of soil gases are effective messengers of the microbial pathways active in the soil.&#160; We have developed and deployed a high frequency sensor consisting of new diffusive soil probes coupled with a Tunable Infrared Laser Direct Absorption Spectrometer (TILDAS) to monitor a range of soil gas species to investigate biogeochemical soil processes.</p><p>An array of soil probes was deployed at the Tropical Rainforest at Biosphere 2 in Arizona as part of the Water Atmosphere and Life Dynamics (WALD) experiment in 2019-2020.&#160; Probes were located in a root zone and nearby control area, and at several depths via a soil pit.&#160; These probes were coupled with a TILDAS to monitor isotopologues of nitrous oxide (N<sub>2</sub>O) including <sup>14</sup>N<sup>15</sup>NO, <sup>15</sup>N<sup>14</sup>NO, N<sub>2</sub><sup>18</sup>O, and methane (<sup>13</sup>CH<sub>4</sub> and <sup>12</sup>CH<sub>4</sub>), as well as CO<sub>2</sub>. During the WALD experiment, the probe-TILDAS system followed the impact on the soil biome of a 2 month induced drought in the rainforest and the subsequent return of rain.&#160; The high temporal resolution of the system allowed us to monitor each probe every 2 hours and thus observe changes in the composition of soil gases that reflect biogeochemical processes and pathways.&#160; CO<sub>2</sub> and thus respiration decreased significantly during the drought and was slow to recover.&#160; Differences in N<sub>2</sub>O mixing ratios and isotopic signatures (both site preference and bulk 15N) in the root zone versus a controlled soil region were observed during both drought and rewetting periods.&#160; Changes in nitrogen and carbon cycles and the microbial pathways during the induced drought and rewetting as reflected in these observations will be discussed.</p>