scholarly journals Antioxidant activity of the probiotic consortium in vitro

2014 ◽  
Vol 2 ◽  
Author(s):  
Saule Saduakhasova ◽  
Almagul Kushugulova ◽  
Samat Kozhakhmetov ◽  
Gulnara Shakhabayeva ◽  
Indira Tynybayeva ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Glutathione Reductase ◽  
Reductase Activity ◽  
Superoxide Dismutase Activity ◽  
Total Antioxidant Activity ◽  
Bacterial Cultures ◽  
Total Antioxidant ◽  
Glutathione Reductase Activity

Introduction: Available evidence suggests that probiotics have different biological functions that depend on several mechanisms, such as antioxidant and DNA-protective activities. The probiotic consortium includes bacterial cultures such as Streptococcus thermophilus, Lactococcus lactis, Lactobacillus plantarum, and other bacterial cultures isolated from traditional Kazakh dairy products (ayran, kumys, shubat, and healthy clinical material). The aim of this study was to investigate the total antioxidant activity of the consortium of probiotic bacteria and to determine the activity of superoxide dismutase, glutathione reductase, and DNA-protective action.Material and methods: In vitro comet assay was used to determine the antigenotoxicity of the probiotic consortium. Total antioxidant activity was determined using a method of analysis with Trolox as the equivalent. The analysis method of superoxide dismutase activity assesses the inhibition rate of the nitroblue tetrazolium reduction to formazan by superoxide dismutase. Determination of glutathione reductase activity is based on the measurement of the NADPH oxidation speed.Results: A significantly high level of the total antioxidant activity of the probiotic consortium intact cells (15.3 mM/ml) was observed whereas the activity index of  lysate  was 11.1 mM/ml.The superoxide dismutase activity of probiotic consortium lysate was evaluated, with values that peaked at 0.24 U/mg protein. The superoxide dismutase activity of the consortium was lower in comparison to L.fernentum E-3 and L.fernentum E-18 cultures with values of 0.85 U/mg and 0.76 U/mg protein, respectively. SOD activity of probiotic consortium whole cells was not observed, which is typical for lactic acid bacteria.Glutathione reductase plays an important role in the optimal protection from oxidative stress. Glutathione reductase activity of the studied probiotic consortium was low; moreover, the activity of the lysate was two times higher than the activity of the cells reaching 0.01 units/ml. Investigations by Dr. Li have shown that the intracellular glutathione may give a significant protection of Lactococcus from the damaging action of H2O2, even at very low concentrations.The data from our study suggests that the co-incubation of the epithelial cells with probiotic bacteria reduces the percentage of damaged cells (damage index–0.60).Conclusion: The studied probiotic consortium has antigenotoxic and antioxidant activities. Preparations and products of this probiotic consortium may serve as a protective component in the intestinal microbial ecosystem. 

scholarly journals Health benefits of new symbiotic “NAR”

2014 ◽  
Vol 2 ◽  
Author(s):  
Saule Saduakhasova ◽  
Almagul Kushugulova ◽  
Samat Kozhakhmetov ◽  
Gulnara Shakhabayeva ◽  
Adil Supiyev ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Glutathione Reductase ◽  
Inflammatory Mediators ◽  
Antioxidant Activities ◽  
Reductase Activity ◽  
Protein A ◽  
Intact Cells ◽  
Total Antioxidant

Introduction: The immune-modulatory effects of synbiotics and their ability to reduce free radical levels may be useful for functional food that is able to be active throughout whole period of colonization of the gastrointestinal tract.The aim of the present study was to investigate the immune-modulatory and antioxidant effects of the synbiotic product "NАR," a probiotic beverage.Methods: The presence of IL-2, IL-4, IL-6, IL-8, IL-10, αTNF, γIFN, Ig A, Ig M, and Ig E was studied in vitro using a solid immunosorbent analysis. The total antioxidant activities of superoxide dismutase and glutathione reductase were determined by a spectrophotometry using the Sigma-Aldrich sets.Results: Studies of the immune-modulatory properties of the synbiotic product NAR showed 1.7 fold increase of γINF levels (p<0.01) in blood after consumption of the synbiotic product “NAR” in comparison to control values, whereas the concentrations of IL-4 and Ig E decreased 2.0 times (treatment: 9.3; control: 18.7; p<0.01) and 1.3 times (p<0.1), respectively. The consumption of the synbiotic product “NAR” caused an increase in the proportion of γINF/IL 4 (treatment: 15.4; control: 4.4; p<0.01), which indicates a reduction in functional activity of Th2-type lymphocytes in comparison with the function of Th1 cells.Our study showed a high level of the total antioxidant activity of the synbiotic product (67.4 mmol/ml). The antioxidant activity of the intact cells of consortium (15.3 mM/ml), which was the basis for the preparation of the symbiotic product, is several times lower than the activity observed in the symbiotic samples.Expression of SOD is one of the mechanisms of antioxidant stress radicals inactivation by bacteria. The analysis identified a superoxide dismutase activity of synbiotic product (1.42 U/mg protein). A glutathione reductase activity of the synbiotic product was elevated (0.06 U/ml). Conclusion: The majority of the inflammatory mediators found in the blood after the consumption of symbiotic product NAR were inflammatory mediators that activate a cellular component of the resistance. Moreover, the symbiotic product has a high antioxidant activity. 

scholarly journals Bioactive compounds content and total antioxidant activity of two savoy cabbages

2014 ◽  
Vol 32 (No. 6) ◽  
pp. 549-554 ◽  
Author(s):  
M.A. Fernández-León ◽  
M. Lozano ◽  
D. González ◽  
M.C. Ayuso ◽  
M.F. Fernández-León
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Antioxidant Activity ◽  
Fresh Weight ◽  
Total Antioxidant ◽  
In Vitro Antioxidant Activity

The bioactive compounds, as well as the in vitro antioxidant activity of two Savoy cabbage cultivars, Dama and Leticia, grown in west of Spain under similar conditions were identified, quantified and compared. We found that cv. Dama presented in general betters results when compared with cv. Leticia. Cv. Dama presented higher concentrations of chlorophyll a (2.26 mg/100 g fresh weight), total phenolic content (102.71 mg of chlorogenic acid equivalent/100 g fresh weight) and total intact glucosinolates (195.22 &micro;mol of sinigrin equivalent/100 g fresh weight). Thus, cv. Dama exhibited higher values of in vitro antioxidant activity. &nbsp;

scholarly journals The relationship of antioxidant enzymes and some physiological parameters in maize during chilling

2011 ◽  
Vol 50 (No. 1) ◽  
pp. 27-32 ◽  
Author(s):  
T. Takáč
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Reductase ◽  
Ascorbate Peroxidase ◽  
Reductase Activity ◽  
Physiological Parameters ◽  
Maize Seedlings ◽  
Maize Cultivars ◽  
Inhibition Of Growth ◽  
Glutathione Reductase Activity

The changes in some physiological parameters of maize seedlings in response to chilling were studied. The emphasis was laid upon their relationship to chilling induced alterations in antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase) activity. The exposure of maize seedlings to chilling caused substantial defects in the 4-day-old seedlings and the seedlings with two fully developed leaves, respectively. The membrane semipermeability perturbations and the loss of viability in the young seedlings were observed. Similarly, we found a decrease of chlorophyll content, appearance of necrotic lesions and inhibition of growth in older plants. The measurements of superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase activities provide an evidence of reactive oxygen species formation, that is assumed to be a reason of the found damages. Significant differences between two cultivars were found in the studied parameters. The electrolyte leakage and viability test provided effective methods for the characterization of the chilling tolerance-level in maize cultivars.

The state of the blood antioxidant system in the patients presenting with acromegaly

2015 ◽  
Vol 61 (2) ◽  
pp. 8-11
Author(s):  
M V Faassen ◽  
M S Pankratova ◽  
N N Molitvoslovova ◽  
A A Baizhumanov ◽  
S S Kovalenko ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Blood Plasma ◽  
Antioxidant System ◽  
The State ◽  
Total Antioxidant Activity ◽  
Sod Activity ◽  
Protein Thiols ◽  
Total Antioxidant

The state of the blood antioxidant system in the patients presenting with acromegaly. The objective of the present study was to evaluate the total antioxidant activity (TAA) of blood plasma, the levels of non-protein thiols and 2-thyobarbituric acid-active products (TBA-AP), superoxide dismutase (SOD) and catalase (CAT) activities as well as ceruloplasmin (CP) level in the patients presenting with acromegaly. It was shown that plasma TAA and SOD activity in this patients was on the average 20 and 30% lower respectively than in the control subjects. At the same time, the TBA-AP and CP levels increased by 50 and 40% respectively. These data suggest the development of oxidative stress in the acromegalic patients.

scholarly journals Increasing Anthocyanin Content in Tomato Fruit for Improved Antioxidant Activity

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 869D-869
Author(s):  
Peter J. Mes* ◽  
James R. Myers
Keyword(s):  
Antioxidant Activity ◽  
Tomato Fruit ◽  
Introgression Line ◽  
Water Soluble ◽  
Carotenoid Content ◽  
Anthocyanin Content ◽  
Total Antioxidant Activity ◽  
Total Antioxidant

Tomato lines carrying the genes Aft, atv, Abg, hp-1, and an as yet undetermined gene from the introgression line LA2099 have been combined to produce fruit with elevated anthocyanin content. The antioxidant activity of juice made from anthocyanin-expressing tomatoes was compared to juices made from tomatoes with varied carotenoid content. The contribution of anthocyanin to the total antioxidant activity of the whole fruit in current material is small, but with potential for significant improvement. The increase in flavonoids in the elevated anthocyanin lines has increased water-soluble antioxidant activity of the fruit in vitro.

scholarly journals Studies on antioxidant activity of different extracts of aerial parts of Cadaba farinose Forsk: An in-vitro techniques

2020 ◽  
Vol 11 (3) ◽  
pp. 2986-2992
Author(s):  
Jambula Dinesh Babu ◽  
Venugopalan Santhosh Kumar
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Radical ◽  
Bioactive Compound ◽  
Antioxidant Potential ◽  
Total Antioxidant Activity ◽  
In Vitro Techniques ◽  
Aerial Parts ◽  
Total Antioxidant ◽  
The Difference

Cadaba farinosa (family Capparidaceae) is generally known as “Indian cadaba” in the traditional ayurvedic system. The current study, aerial parts of different concentrates (Pet.ether, ethyl acetate and methanol) of Cadaba farinose was evaluated for its in-vitro antioxidant potential by Diphenylpicrylhydrazyl radical, and total antioxidant activity taking ascorbate as standard and superoxide radical activity taking Quercetin as the standard for the in-vitro methods. The methanolic concentrates of Cadaba farinose& ascorbic acid exhibited antioxidant potential possessing IC50 208µg/ml & 66µg/ml (Diphenylpicrylhydrazyl radical) , 188µg/ml & 57µg/ml (total antioxidant). The methanolic concentrates of Cadaba farinose & quercetin exhibited antioxidant potential possessing IC50 252µg/ml & 60µg/ml (superoxide radical). The IC50 value was originated that methanolic concentrates of Cadaba farinose more efficient in Diphenylpicrylhydrazyl radical, superoxide radical activity, total antioxidant activity compared EA & PE concentrates. The difference in scavenging potential of the extracts can be due to variation in the percentage of bioactive compound present in different solvents. Invitro antioxidant studies show methanolic concentrates of Cadaba farinose have better antioxidant activity. This result indicates that aerial parts of methanolic concentrate Cadaba farinose could serve as a natural antioxidant, which may be useful in preventing free radical-induced diseases.

scholarly journals Serum biochemistry and oxidative stress indicators in West African dwarf goats under semi intensive management system

2020 ◽  
Vol 44 (4) ◽  
pp. 110-117
Author(s):  
U. G. D. Ihejirika ◽  
O. O. Adenekan ◽  
O. A. Jimoh ◽  
A. A. Jack ◽  
U. C. Uwaeziozi
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Alkaline Phosphatase ◽  
Superoxide Dismutase ◽  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
Serum Biochemistry ◽  
Total Antioxidant Activity ◽  
Intensive Management ◽  
Total Antioxidant

 This study aims to evaluate variations in serum biochemistry and oxidative status between male and female WestAfrican dwarfgoats under semi intensive management system. Twenty, each of bucks and does (12 months old) were randomly selected from a herd purchased from Oyo livestock market, in Oyo State. The average body weights of the goats were12.08 ± 3.26 and 12.15 ± 1.51kg does and bucks, respectively. Blood samples were collected from fastened goats and assayed for serum biochemicals, lipid peroxidation,  total antioxidant activity, catalase and superoxide dismutase using standard procedures. Data obtained were subjected to T-test. The result showed serum glucose, albumin,  cholesterol, total protein, globulin, triglyceride, creatinine, alanine amino transferase(ALT),aspartate amino transferase(AST) and urea were not significantly (P>0.05) different among the sexes. Serum high density lipoprotein was significantly (P<0.05) higher in does (13.96 mmol/l) than in bucks (5.91mmol/l) and alkaline phosphatase was significantly (P<0.05) higher in bucks (2580.55 IU/L) than in does (1594.58 IU/L). Serum lipid peroxidation, total antioxidant activity and superoxide dismutase were statistically similar (P>0.05) in both sexes. Serum catalase was significantly (P<0.05) higher in bucks than in does. From this study, it can be deduced that serum biochemical indices in does and bucks are similar except for alkaline phosphatase and high density lipoprotein. Bucks recorded higher catalase than does.It could be concluded that during physiological and environmental stress conditions, antioxidant supplementation should be adopted to boost the antioxidant capacity of the does.

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