scholarly journals Study of Some Physiological and Genetic Changes Using the Soybean (Glicine max (L) MEER) Test System

2021 ◽  
Author(s):  
Zhana Chitanava ◽  
Nana Zarnadze
Keyword(s):  
Mutagenic Activity ◽  
High Stress ◽  
Test System ◽  
Dose Effect ◽  
Stress Index ◽  
Physical Factors ◽  
Anthropogenic Pressure ◽  
Genetic Line ◽  
Phenotypic Effect ◽  
Genetic Changes

Anthropogenic pressure on the biosphere has become a common process of the 21st century. Among substances synthesized by humans, genotoxic agents which include pesticides are considered to be particularly dangerous. The number of pesticides used in agriculture is gradually increasing, accumulating and circulating in the biosphere. The use of pesticides is accompanied by their involvement in food chains and accumulation in individuals. They are characterized by a fairly high stress index and cause genetic changes in living organisms. Various test methods have been developed to study these issues. B. Wig and Al. Podok suggested a genetic line for soybean containing a mutation of the chlorophyll-synthesizing gene. The genetic line is characterized by a phenotypic effect. Indicator, genetic line owner soybean, diploid, heterozygous, give three phenotypically different sprouts: green (genotype Y11 Y11), lettuce-color (genotype Y11 y11) and yellow (genotype y11 y11). 1: 2: 1 ratio is observed between the sprouts. This type of ratio is typical for incomplete dominance. Through the spots detected on the leaves it is possible to study the recombinogenic and mutagenic activity in soybean induced by chemical and physical factors and to record the induced changes in somatic cell. Using these systems, we first studied the effects of pesticides karate and Bordeaux on plant growth and sprouting processes and the genetic changes induced by their influence. Both pesticides had an inhibitory effect on physiological processes, also, the frequency of direct mutations was determined by the "dose-effect" phenomenon.

scholarly journals Assessment of the Mutagenic Activity of Extracts of Brazilian Propolis in Topical Pharmaceutical Formulations on Mammalian CellsIn VitroandIn Vivo

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Juliana Marques Senedese ◽  
Aline Rafaela Rodrigues ◽  
Michelle Andrade Furtado ◽  
Viviane Dias Faustino ◽  
Andresa A. Berretta ◽  
...  
Keyword(s):  
Chromosomal Aberrations ◽  
Mutagenic Activity ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Test System ◽  
Negative Control ◽  
Continuous Treatment ◽  
Mutagenic Potential ◽  
Topical Formulations

Propolis possesses various biological activities such as antibacterial, antifungal, anti-inflammatory, anesthetic and antioxidant properties. A topically applied product based on Brazilian green propolis was developed for the treatment of burns. For such substance to be used more safely in future clinical applications, the present study evaluated the mutagenic potential of topical formulations supplemented with green propolis extract (1.2, 2.4 and 3.6%) based on the analysis of chromosomal aberrations and of micronuclei. In thein vitrostudies, 3-h pulse (G1phase of the cell cycle) and continuous (20 h) treatments were performed. In thein vivoassessment, the animals were injured on the back and then submitted to acute (24 h), subacute (7 days) and subchronic (30 days) treatments consisting of daily dermal applications of gels containing different concentrations of propolis. Similar frequencies of chromosomal aberrations were observed for cultures submitted to 3-h pulse and continuous treatment with gels containing different propolis concentrations and cultures not submitted to any treatment. However, in the continuous treatment cultures treated with the 3.6% propolis gel presented significantly lower mitotic indices than the negative control. No statistically significant differences in the frequencies of micronuclei were observed between animals treated with gels containing different concentrations of propolis and the negative control for the three treatment times. Under the present conditions, topical formulations containing different concentrations of green propolis used for the treatment of burns showed no mutagenic effect in either test system, but 3.6% propolis gel was found to be cytotoxic in thein vitrotest.

scholarly journals Occupational Stress and Job Burnout among Primary and Secondary School Teachers in Cuttack, Orissa, 2013

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Joyce Mathews
Keyword(s):  
Occupational Stress ◽  
Secondary School Teachers ◽  
Demographic Data ◽  
High Stress ◽  
Job Burnout ◽  
Stress Index ◽  
Stress Levels ◽  
Older Teachers ◽  
The Relationship ◽  
Primary And Secondary School

The purpose of this study was to explore the levels of occupational stress and job burnout among teachers working in primary and secondary schools and identify the relationship between the selected demographic data with the stress levels. The sample consisted of 100 teachers from 4 schools in Cuttack, Orissa. The instruments used to measure the variables were: 1.Headington Stress Index questionnaire, 2.Headingtacon Burnout Inventory, 3. Self care and Lifestyle Balance Inventory. The statistical techniques of descriptive statistics, ANOVA analysis and Pearson’s coefficient were employed to analyze the data. The findings of the study indicated that a major portion of the teachers’ population faced moderately low stress and burnout, and the remaining faced moderately high stress and burnout. Younger teachers are reported to have more stress than the older teachers. Higher the burnout among teachers, lower was the lifestyle balance among them. Experience, gender and grade did not affect the stress levels among teachers.

scholarly journals Study on the Effect of Laser Quenching on Fretting Fatigue Life

Metals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 566 ◽  
Author(s):  
Hongjian Zhang ◽  
Xufeng Yang ◽  
Haitao Cui ◽  
Weidong Wen
Keyword(s):  
Fatigue Life ◽  
Stress Level ◽  
High Stress ◽  
Fretting Fatigue ◽  
Test System ◽  
Experimental Results ◽  
Laser Quenching ◽  
Error Band ◽  
Hydraulic Servo ◽  
High Stress Level

Laser quenching hardening is one of the most used surface treated technologies. In order to study the effect of laser quenching on the fretting fatigue life, fretting fatigue experiments of TC11 (Ti-6.5Al-1.5Zr-3.5Mo-0.3Si) titanium alloy specimens with different surface conditions were carried out on a special hydraulic servo fatigue test system. The experimental results showed that laser quenching hardening has a good performance in increasing the fretting fatigue lives of the TC11 alloy. However, the effects of laser quenching on fretting fatigue are more obviously at low stress level than at high stress level, the fretting fatigue life was increased by 110.78% at low stress level and 17.56% at high stress level, respectively. Based on the critical plane approach, the traditional SWT (Smith–Watson–Topper) parameter was modified and used to describe the fretting fatigue life of the TC11 alloy after hardening by the consideration of the variations of the hardening layer’s elastic modulus. Compared with the experimental results, all the errors of the predicted results lied in the error band of two.

scholarly journals Toxic and mutagenic activities of surface water from the Chumysh River

2017 ◽  
Vol 7 (2) ◽  
pp. 104-109
Author(s):  
L. P. Khlebova ◽  
N. N. Chernysheva ◽  
A. P. Kraynov
Keyword(s):  
River Water ◽  
Chromosomal Abnormalities ◽  
Mutagenic Activity ◽  
Tap Water ◽  
Control Sample ◽  
Test System ◽  
Root Tips ◽  
Allium Test ◽  
Temporal And Spatial Distribution ◽  
Onion Root

<p>The Allium test system is worldwide used for biological monitoring of environmental pollutants. We have studied toxic and mutagenic activity of water from the Chumysh River, which is one of the largest tributaries of the Upper Ob. Water samples were taken near industrial settlement Talmenka (Altai Territory of Russia) during the spring flood and low autumn water level in 2015. Root tips of <em>Allium cepa</em> L. was used as a biological model. There were examined 30 samples. Purified tap water of medium hardness served as a control sample. After the synchronizing the cell divisions, the onion bulbs with roots reaching a length of 2-3 mm were transferred to glass cups containing the selected samples of River water and cultured for several days at a temperature of +24 ± 1°C. After exposure, root tips were rinsed in distilled water and fixed in a cold mixture of ethanol and acetic acid (3:1). Fixed samples were used after 12-24 h or transferred to 70% alcohol and stored in refrigerator at a temperature of +4°C until required. The fixed materials were hydrolyzed in 1N HCl at 60°C for 5-8 min and squashed in aceto-orcein.<strong> </strong>Prepared slides were viewed under the microscope at a magnification of ×90.<strong> </strong>The mitotic index (MI), the phase indices, the frequency of abnormal mitosis, and chromosomal aberrations were determined by the examination of 500 cells per a replicate (100 cells per slide). We established that the decreased or increased levels of mitotic activity and the frequencies of pathological mitoses (up to 7.9%, P &lt; 0.05) in onion root tips revealed the presence of mitotoxic and genotoxic agents in the Chumysh River water. We found that the most number of chromosomal abnormalities occurs at the stages of meta- or anaphase. The main abnormalities are chromosome laggings in meta- and anaphase, chromosome bridges, chromosome fragments and micronuclei. Their number increased in 5.0-10.8 times compared with the control value. It has been discovered the temporal and spatial distribution of compounds with different toxicity and genotoxicity within a stream. The greatest level of mitotic depression and the highest frequency of chromosomal mutations were observed in the tissues of the onion root tips germinated on the samples collected in low autumn water. The mechanisms of plant adaptation to unfavorable environmental factors are discussed.</p>

Carer stress in Fetal Alcohol Spectrum Disorders: the implications of data from the UK national specialist FASD clinic for training carers

2020 ◽  
Vol 44 (3) ◽  
pp. 242-254
Author(s):  
Zameer Mohamed ◽  
Alexandra CS Carlisle ◽  
Alexandra C Livesey ◽  
Raja AS Mukherjee
Keyword(s):  
Parental Stress ◽  
Fetal Alcohol Spectrum Disorders ◽  
High Stress ◽  
Stress Index ◽  
Adaptive Behaviour ◽  
Service Development ◽  
Fetal Alcohol ◽  
Spectrum Disorders ◽  
The Uk ◽  
Fetal Alcohol Spectrum

Medical research literature is increasingly reporting high levels of stress among carers of children with Fetal Alcohol Spectrum Disorders (FASD). However, while there are a growing number of evidence-based innovations around the world for carer stress generally, there are no programmes in the UK for those looking after children with FASD. The aim of this study, therefore, is to provide an up-to-date profile of stress faced by these carers, to explore its sources and suggest directions for service development. Seventy-one carers and their child with FASD participated in a study designed for this purpose. Parental stress levels were assessed using the Parental Stress Index (PSI) and the characteristics of their children – cognition, adaptive behaviour, sensory processing and externalising behaviour function – by a battery of measures. Further correlational and regression analyses were carried out to explore the nature and source of the carer stress identified. When the levels of stress in families was measured, all six Child Domains on the PSI showed results that were above the clinically significant cut-off for high stress, while all seven Parent Domains were below this threshold. Significant associations were found between the PSI and child behaviours, but generally the Parent Domain and Child Behaviours were not correlated. Regression analysis found executive functioning difficulties in children to be the main predictors of carer stress but sensory difficulties were not significant, despite 83% of the children having elevated problems of this kind. Total stress scores among carers of children with FASD were elevated and far exceeded the threshold score on the PSI, suggesting a need for ‘further professional consultation’. Consideration of parental needs and the development of evidenced-based interventions specific for these carers are highlighted as areas for future development.

scholarly journals Parallel genomic architecture underlies repeated sexual signal divergence in Hawaiian Laupala crickets

2019 ◽  
Author(s):  
Thomas Blankers ◽  
Kevin P. Oh ◽  
Kerry L. Shaw
Keyword(s):  
Major Effect ◽  
Male Mating ◽  
Phenotypic Effect ◽  
Genetic Changes ◽  
Species Pairs ◽  
Song Divergence ◽  
Theoretical Predictions ◽  
Phenotypic Distance ◽  
Cricket Song ◽  
Signal Divergence

ABSTRACTWhen the same phenotype evolves repeatedly, we can explore the predictability of genetic changes underlying phenotypic evolution. Theory suggests that genetic parallelism is less likely when phenotypic changes are governed by many small-effect loci compared to few of major effect, because different combinations of genetic changes can result in the same quantitative outcome. However, some genetic trajectories might be favored over others, making a shared genetic basis to repeated polygenic evolution more likely. To examine this, we studied the genetics of parallel male mating song evolution in the Hawaiian cricket Laupala. We compared quantitative trait loci (QTL) underlying song divergence in three species pairs varying in phenotypic distance. We tested whether replicated song divergence between species involves the same QTL and the likelihood that sharing QTL is related to phenotypic effect sizes. Contrary to theoretical predictions, we find substantial parallelism in polygenic genetic architectures underlying repeated song divergence. QTL overlapped more than expected based on simulated QTL analyses. Interestingly, QTL effect size did not predict QTL sharing, but did correlate with magnitude of phenotypic divergence. We highlight potential mechanisms driving these constraints on cricket song evolution and discuss a scenario that consolidates empirical quantitative genetic observations with micro-mutational theory.

scholarly journals Deep-Learning-Based Stress-Ratio Prediction Model Using Virtual Reality with Electroencephalography Data

2020 ◽  
Vol 12 (17) ◽  
pp. 6716
Author(s):  
Seung Yeul Ji ◽  
Se Yeon Kang ◽  
Han Jong Jun
Keyword(s):  
Deep Learning ◽  
Prediction Model ◽  
Design Process ◽  
Architectural Design ◽  
Stress Ratio ◽  
High Stress ◽  
Stress Index ◽  
Brain Wave ◽  
Learning Platform ◽  
High Stress Level

The Reich Chancellery, built by Albert Speer, was designed with an overwhelming ambience to represent the worldview of Hitler. The interior of the Reich Chancellery comprised high-ceiling and low-ceiling spaces. In this study, the change in a person’s emotions according to the ceiling height while moving was examined through brain wave experiments to understand the stress index for each building space. The Reich Chancellery was recreated through VR, and brain wave data collected per space were processed through a first and second analysis. In the first analysis, beta wave changes related to the stress index were calculated, and the space with the highest fluctuation was analyzed. In the second analysis, the correlation between 10 different types of brain waves and waveforms was analyzed; deep-learning algorithms were used to verify the accuracy and analyze spaces with a high stress index. Subsequently, a deep-learning platform for calculating such a value was developed. The results showed that the change in stress index scores was the highest when entering from the Mosaic Hall (15 m floor height) to the Führerbunker (3 m floor height), which had the largest floor height difference. Accordingly, a stress-ratio prediction model for selecting a space with a high stress level was established by monitoring the architectural space based on brain wave information in a VR space. In the architectural design process, the ratio can be used to reflect user sensibility in the design and improve the efficiency of the design process.

scholarly journals Experimental and numerical investigation of the effect of interlayer on the damage formation in a ceramic/composite armor at a low projectile velocity

2016 ◽  
Vol 30 (1) ◽  
pp. 88-106 ◽  
Author(s):  
A Tasdemirci ◽  
G Tunusoglu
Keyword(s):  
Stress Wave ◽  
High Stress ◽  
Test System ◽  
Wave Transmission ◽  
Front Face ◽  
Low Velocity ◽  
Projectile Impact ◽  
Backing Plate ◽  
Composite Armor ◽  
The Impact

The damage formation in a multilayered armor system without and with an interlayer (rubber, Teflon, and aluminum foam) between the front face ceramic layer and the composite backing plate were investigated experimentally and numerically. The projectile impact tests were performed in a low-velocity projectile impact test system and the numerical studies were implemented using the nonlinear finite element code LS-DYNA. The results of numerical simulations showed that the stress wave transmission to the composite backing plate decreased significantly in Teflon and foam interlayer armor configurations. Similar to without interlayer configuration, the rubber interlayer configuration led to the passage of relatively high stress waves to the composite backing plate. This was mainly attributed to the increased rubber interlayer impedance during the impact event. The numerical results of reduced stress wave transmission to the backing plate and the increased damage formation in the ceramic front face layer with the use of Teflon and foam interlayer was further confirmed experimentally.

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