scholarly journals PREPARATION OF A DEGRADABLE HYDROCOLOID FILM WITH SILVER NANOPARTICLES SYNTHETIZED WITH AQUEOUS GARLIC EXTRACT (Allium sativum)

2018 ◽  
Vol 15 (29) ◽  
pp. 266-275
Author(s):  
F. PILAQUINGA ◽  
V. ESCOBAR ◽  
G. CUEVA ◽  
L. MENESES
Keyword(s):  
Silver Nanoparticles ◽  
Allium Sativum ◽  
Atomic Absorption Spectrophotometry ◽  
Gelatin Film ◽  
Garlic Extract ◽  
Scanning Microscopy ◽  
Transmission Microscopy ◽  
Bactericidal Properties ◽  
Electron Scanning Microscopy ◽  
Electron Transmission Microscopy

The green synthesis of silver nanoparticles aims to synergistically bind bactericidal properties of the element with the chemical composition of plant extracts and potentially use them in biodegradable plastic substitute materials. In this work, a degradable gelatin film was prepared with silver nanoparticles synthesized with aqueous extract of garlic (Allium sativum); the nanoparticles were previously characterized by ultraviolet-visible spectrophotometry, electron scanning microscopy and electron transmission microscopy. To make the film, pure gelatin, gelatin - carboxymethylcellulose - glycerol, and gelatin - arabic gum mixtures were tested, obtaining the desired texture only with gelatin, at different concentrations. The film with the greatest flexibility was 10% gelatin, with puncture resistance of 47 N and density of 0.943 g/mL. This film was analyzed by infrared spectroscopy, finding modifications of the spectrum dependent on the presence of silver nanoparticles-garlic extract. The electron scanning microscopy revealed a uniform distribution of silver in the film (74.4nm) with an abundance of 56.20% and a concentration of 1.58 mg/g determined by atomic absorption spectrophotometry. Finally, the prepared gelatin film is resistant, amber colored, with possible bactericidal properties due to the presence of silver nanoparticles and easily degradable at 50°C in water.

Ultrastructure of Melanin Formation in Curvularia sp., Alternaria sp., and Drechslera Sorokiniana

1977 ◽  
Vol 35 ◽  
pp. 398-399
Author(s):  
M. H. Wheeler ◽  
W. J. Tolmsoff ◽  
A. A. Bell
Keyword(s):  
Potassium Phosphate ◽  
Thielaviopsis Basicola ◽  
Wild Type ◽  
Potassium Phosphate Buffer ◽  
Transmission Microscopy ◽  
Electron Transmission ◽  
Melanin Formation ◽  
Before And After ◽  
Alternaria Sp ◽  
Electron Transmission Microscopy

(+)-Scytalone [3,4-dihydro-3,6,8-trihydroxy-l-(2Hj-naphthalenone] and 1,8-di- hydroxynaphthalene (DHN) have been proposed as intermediates of melanin synthesis in the fungi Verticillium dahliae (1, 2, 3, 4) and Thielaviopsis basicola (4, 5). Scytalone is enzymatically dehydrated by V. dahliae to 1,3,8-trihydroxynaphthalene which is then reduced to (-)-vermelone [(-)-3,4- dihydro-3,8-dihydroxy-1(2H)-naphthalenone]. Vermelone is subsequently dehydrated to DHN which is enzymatically polymerized to melanin.Melanin formation in Curvularia sp., Alternaria sp., and Drechslera soro- kiniana was examined by light and electron-transmission microscopy. Wild-type isolates of each fungus were compared with albino mutants before and after treatment with 1 mM scytalone or 0.1 mM DHN in 50 mM potassium phosphate buffer, pH 7.0. Both chemicals were converted to dark pigments in the walls of hyphae and conidia of the albino mutants. The darkened cells were similar in appearance to corresponding cells of the wild types under the light microscope.

EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

2019 ◽  
Vol 14 (1) ◽  
pp. 26
Author(s):  
Dyah Anggraeni ◽  
Nurlela Nurlela
Keyword(s):  
Room Temperature ◽  
Allium Sativum ◽  
Research Result ◽  
Clarias Gariepinus ◽  
Storage Period ◽  
The Body ◽  
Garlic Extract ◽  
African Catfish ◽  
Natural Preservatives ◽  
Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L).  Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus).  Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter.  Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

Garlic Extract (Allium sativum L.) Effectively Inhibits Staphylococcus aureus and Escherichia coli by Invitro Test

2020 ◽  
Vol 2 (2) ◽  
pp. 61-68
Author(s):  
Agnina Listya Anggraini ◽  
Ratih Dewi Dwiyanti ◽  
Anny Thuraidah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Infections ◽  
Allium Sativum ◽  
Antibacterial Properties ◽  
Herbal Medicines ◽  
Garlic Extract ◽  
Dilution Method ◽  
Initial Stage

Infection is a disease caused by the presence of pathogenic microbes, including Staphylococcus aureus and Escherichia coli. Garlic (Allium sativum L.) has chemical contents such as allicin, alkaloids, flavonoids, saponins, tannins, and steroids, which can function as an antibacterial against Staphylococcus aureus and Escherichia coli. This study aims to determine the antibacterial properties of garlic extract powder against Staphylococcus aureus and Escherichia coli. This research is the initial stage of the development of herbal medicines to treat Staphylococcus aureus and Escherichia coli infections. The antibacterial activity test was carried out by the liquid dilution method. The concentrations used were 30 mg/mL, 40 mg/mL, 50 mg/mL, 60 mg/mL and 70 mg/mL. The results showed that the Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus and Escherichia coli was 40 mg/mL and 50 mg / mL. Minimum Bactericidal Concentration (MBC) results for Staphylococcus aureus and Escherichia coli are 50 mg/mL and 70 mg/mL. Based on the Simple Linear Regression test, the R2 value of Staphylococcus aureus and Escherichia coli is 0.545 and 0.785, so it can be concluded that there is an effect of garlic extract powder on the growth of Staphylococcus aureus and Escherichia coli by 54.5% and 78.5%. Garlic (Allium sativum L.) extract powder has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.

Antibacterial activity of garlic extract and silver Nanoparticles: an in vitro analysis

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
SHREYASHI M ◽  
SULAGNA D ◽  
SANKARI D ◽  
THIRUMURUGAN D ◽  
INFANT SANTHOSE B ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Garlic Extract ◽  
Vitro Analysis ◽  
In Vitro Analysis

The effect of garlic extract (Allium sativum L.) (Amaryllidaceae) to eradicate the Porphyromonas endodontalis biofilm: An in-vitro research

2021 ◽  
Vol 5 (3) ◽  
pp. 138
Author(s):  
AdiDharma Widjaya ◽  
MeinyFaudah Amin ◽  
Aryadi ◽  
BoediOetomo Roeslan
Keyword(s):  
Allium Sativum ◽  
Garlic Extract

scholarly journals Протонпроводящие композиционные материалы на основе соединения Cs-=SUB=-6-=/SUB=-H(HSO-=SUB=-4-=/SUB=-)-=SUB=-3-=/SUB=-(H-=SUB=-2-=/SUB=-PO-=SUB=-4-=/SUB=-)-=SUB=-4-=/SUB=-

2019 ◽  
Vol 61 (12) ◽  
pp. 2375
Author(s):  
В.А. Коморников ◽  
В.В. Гребенев ◽  
И.С. Тимаков ◽  
О.Б. Зайнуллин
Keyword(s):  
Impedance Spectroscopy ◽  
Transport Properties ◽  
Phase Analysis ◽  
Composition Range ◽  
Scanning Microscopy ◽  
Proton Conducting ◽  
X Ray ◽  
Conducting Materials ◽  
Electron Scanning Microscopy

Composite proton-conducting materials xCs6H (HSO4) 3 (H2PO4) 4+ (1 - x) AlPO4 were obtained in the composition range (x = 0.9–0.7). The methods of x-ray phase analysis, impedance spectroscopy, electron scanning microscopy studied their physicochemical and transport properties.

scholarly journals IDENTIFIKASI SENYAWA GARLISIN DAN KUERSETIN SEBAGAI SENYAWA ANTIMIKROBA PADA SEDIAAN BASAH DAN KERING BAWANG PUTIH (Allium sativum L.)

2020 ◽  
Vol 8 (2) ◽  
pp. 176
Author(s):  
Fanny Thresia Yunus ◽  
Ari Suwondo ◽  
Martini Martini
Keyword(s):  
Traditional Medicine ◽  
Allium Sativum ◽  
Chemical Compounds ◽  
The Body ◽  
Garlic Extract ◽  
Antimicrobial Compounds ◽  
Vacuum Oven ◽  
Garlic Powder ◽  
Three Samples

Indonesian people have long used garlic as traditional medicine because it contains various chemical compounds that are beneficial to the body. In this study identification of garlicin and kuersetin compounds in garlic as an antibacterial. This study requires 500 grams of garlic each in wet preparations consist of fine garlic and garlic extract, and dry preparation consists of garlic powder. In addition, this research requires 2,1 liters of 96% ethanol. Garlic is dried at 400C with TMI Vacuum Oven and extracted at 250C. Garlic powder is made using Krisbow dry cabinet with a temperature of 1000F and a pressure of 10 Pa. Garlisin content was identified by GCMS Shimadzu TQ8030 and FTIR Perkin Elmer Spectrum Two UATR, while identification of quercetin using Shimadzu HPLC type UFLC. The result shows that the compound garlicin (C6H10S2) was found in the three samples with identical similarity levels. In addition, the three samples showed differences in kuersetin content. The highest kuersetin content was 458.729 ppm garlic extract while the lowest kuersetin content was 81.181 ppm fresh garlic. Both of these compounds have potential as antimicrobial compounds.

scholarly journals Broncho-alveolar inflammation in COVID-19 patients: a correlation with clinical outcome

2020 ◽  
Author(s):  
laura pandolfi ◽  
Fossali Tommaso ◽  
Frangipane Vanessa ◽  
Bozzini Sara ◽  
Morosini Monica ◽  
...  
Keyword(s):  
Clinical Outcome ◽  
Mononuclear Cells ◽  
Severe Pneumonia ◽  
Nasopharyngeal Swab ◽  
Icu Patients ◽  
Transmission Microscopy ◽  
Inflammatory Status ◽  
Viral Particles ◽  
A Prospective Study ◽  
Electron Transmission Microscopy

Abstract Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rapidly reached pandemic proportions. Given that the main target of SARS-CoV-2 are lungs leading to severe pneumonia with hyperactivation of the inflammatory cascade, we conducted a prospective study to assess alveolar inflammatory status in patients with moderate to severe COVID-19. Methods: Diagnostic bronchoalveolar lavage (BAL) was performed in 33 adult patients with SARS-CoV-2 infection by real-time PCR on nasopharyngeal swab admitted to the Intensive care unit (ICU) (n=28) and to the Intermediate Medicine Ward (IMW) (n=5). We analyze the differential cell count, ultrastructure of cells and Interleukin(IL)6, 8 and 10 levels.Results: ICU patients showed a marked increase in neutrophils (1.24 x 105 ml-1 , 0.85-2.07), lower lymphocyte (0.97 x 105 ml-1, 0.024-0.34) and macrophages fractions (0.43 x 105 ml-1, 0.34-1.62) compared to IMW patients (0.095 x 105 ml-1, 0.05-0.73; 0.47 x 105 ml-1, 0.28-1.01 and 2.14 x 105 ml-1, 1.17-3.01, respectively) (p<0.01). Study of ICU patients BAL by electron transmission microscopy showed viral particles inside mononuclear cells confirmed by immunostaining with anti-viral capsid and spike antibodies. IL6 and IL8 were significantly higher in ICU patients than in IMW (IL6 p<0.01, IL8 p<0.0001), and also in patients who did not survive (IL6 p < 0.05, IL8 p = 0.05 vs. survivors). IL10 did not show a significant variation between groups. Dividing patients by treatment received, lower BAL concentrations of IL6 were found in patients treated with steroids as compared to those treated with tocilizumab (p<0.1) or antivirals (p<0.05). Conclusions: Alveolitis, associated with COVID-19, is mainly sustained by innate effectors which showed features of extensive activation. The burden of pro-inflammatory cytokines IL6 and IL8 in the broncho-alveolar environment is associated with clinical outcome.

Sign in / Sign up

Export Citation Format

Share Document