scholarly journals Development of bioanalytical standardization parameters of Alocasia indica tuber by HPTLC technique

2020 ◽  
pp. 295-299
Author(s):  
Roman Kumar Aneshwari ◽  
ANIL KUMAR SAHU ◽  
Amber Vyas ◽  
VISHAL JAIN
Keyword(s):  
Formic Acid ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
Ethanolic Extract ◽  
Solvent System ◽  
Perennial Herb ◽  
Present Species ◽  
Active Components ◽  
Hptlc Method ◽  
Routine Quality Control

Alocasia indica is perennial herb growing widely and used as traditional medicine in India, China and Bangladesh. The divine herb has potent medicinal values for the treatment of different type of illnesses. The HPTLC techniques were used to separate active components from ethanolic extract of tuber part of A. indica. This examination was intended to designed a HPTLC fingerprint profile of crude extract of the plant in ethanol. A HPTLC method for the isolation of various active constituents in A. indica ethanolic extract have been developed and solvent system for quercetin the mobile phase used was toluene: ethyl acetate: formic acid (5:2:1) and for analysis of β-sitosterol the mobile phase used was chloroform: ethyl acetate: formic acid (6:4:1) . In the present investigation, HPTLC fingerprint of extract of dried tuber part of A.  indica have been performed and the results demonstrated that important  information for standardization. The HPTLC system for routine quality control of present species can be used for ethanolic extract and serve in qualitative, quantitative and was appropriate for standardization of the plant.

STANDARDIZATION OF HYGROPHILA SPINOSA AND ITS FORMULATION WITH REFERENCE TO LUPEOL, BY DEVELOPED AND VALIDATED HPTLC AND RP-HPLC METHODS

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (01) ◽  
pp. 13-19
Author(s):  
R Jayaprakasam ◽  
◽  
M. F. Saleshier ◽  
T. K. Ravi
Keyword(s):  
Standard Deviation ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
Solvent System ◽  
Good Recovery ◽  
Rp Hplc ◽  
Relative Standard ◽  
Optimum Wavelength ◽  
Hptlc Method

The separation and determination of lupeol from Hygrophila spinosa were carried out by two simple, precise and accurate HPTLC and RP-HPLC methods. HPTLC method for the determination of lupeol from plant extract and its formulation was developed using a solvent system consisting of toluene : ethyl acetate : methanol (15: 3: 1.5%v/v/v). For detection, lupeol had to be derivatized with Liebermann Burchard reagent at 1050C. The optimum wavelength was fixed as 366nm. In RP-HPLC, the separation was carried out on a C18 column and the mobile phase selected was methanol: acetonitrile (30:70%v/v). The maximum wavelength was found to be 210nm.The method was validated in terms of various parameters. Low relative standard deviation and good % recovery values of both the methods showed that the developed methods were highly precise and accurate and therefore can be used for the standardization and quantification of lupeol in Hygrophila spinosa and its formulation.

A Study on the variation of Apigenin content in Cardiospermum halicacabum from 21 districts of Tamil Nadu by HPTLC

2021 ◽  
pp. 2613-2618
Author(s):  
A Rajasekaran ◽  
V Abirami ◽  
S Arunkumar ◽  
Priya G Bharani ◽  
S Gugapriya ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Stationary Phase ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
Tamil Nadu ◽  
Leaf Extract ◽  
Major Constituent ◽  
Marker Compound ◽  
Aluminium Sheets ◽  
Hptlc Method

Cardiospermum halicacabum is one of the most potent medicinal plants used in Indian traditional systems of medicine for the treatment of various diseases, mainly for arthritis. Apigenin is one of the major constituent present in Cardiospermum halicacabum. The present study mainly aimed to estimate the content of major constituent apigenin present in Cardiospermum halicacabum collected from 21 districts of Tamil Nadu by HPTLC method using the marker compound apigenin. The HPTLC method was performed using HPTLC aluminium sheets precoated with Silica Gel 60 GF254 as stationary phase and Toluene: Ethyl acetate: formic acid: methanol (3:6:1.6:0.4 v/v) as the mobile phase. The developed chromatogram was scanned at 254nm using Camag Scanner III. The Rf value of standard apigenin and apigenin in the leaf extract of Cardiospermum halicacabum was found to be in the range of 0.80 to 0.89. Plant collected from Cuddalore district of Tamil Nadu was found to contain relatively high amount of marker compound apigenin than other regions.

DEVELOPMENT OF HPTLC METHOD FOR SIMULTANEOUS ESTIMATION OF CIPROFLOXACIN AND ORNIDAZOLE IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (05) ◽  
pp. 36-43
Author(s):  
N. R Dighade ◽  
◽  
M. D Shende ◽  
A. V Kasture
Keyword(s):  
Quality Control ◽  
High Resolution ◽  
Thin Layer ◽  
Mobile Phase ◽  
High Performance ◽  
Dosage Form ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Hptlc Method ◽  
Routine Quality Control

A simple and accurate high performance thin layer chromatographic (HPLTC) method has been developed and validated as per ICH guidelines for estimations of Ciprofloxacin (CP) and Ornidazole (ORN) in combined dosage form. The mobile phase was acetonitrile: toluene: water and triethylamine (5.5:1.8:1.5:1.6 V/V) was found to be best which gave high resolution with Rf 0.16 and 0.84 for ciprofloxacin and ornidazole respectively. The linearity of ciprofloxacin and ornidazole was found to be in the range of 0.4 to 0.8 µg/mL and 0.4 to 0.8 µg/mL, respectively. The coefficient of correlation (r2 ) was found to be greater than 0.989 for both the components by this method. The tablet analyses result (n = 5) were found to be > 100.84 % by HPTLC for both the components. The proposed method was found to be simple, accurate and suitable for routine quality control of marketed formulations containing these drugs.

ANALYTICAL METHOD DEVELOPMENT AND ITS VALIDATION FOR EVALUATION OF POLYHERBAL FORMULATION

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (10) ◽  
pp. 66-68
Author(s):  
◽  
A. P Jadhav
Keyword(s):  
Immune Response ◽  
Ethyl Acetate ◽  
Analytical Method ◽  
Mobile Phase ◽  
Method Development ◽  
Simultaneous Estimation ◽  
Active Constituents ◽  
Polyherbal Formulation ◽  
Marker Compounds ◽  
Hptlc Method

Talekt capsule is a branded polyherbal formulation used for enhancing the immune response to dermal infections and also for treating skin disorders. Curcumin and embelin, which are the active constituents of Haridra and Vidanga, respectively were used as marker compounds for developing a simple, accurate, precise and robust HPTLC method for simultaneous estimation. The mobile phase of toluene: ethyl acetate: formic acid (6.5: 3: 0.2 v/v/v) was used for separation. 381nm was used as wavelength for analysis. The Rf value obtained for curcumin, and embelin was found to be 0.51 ± 0.2 and 0.33 ± 0.2, respectively. The developed method was validated on the basis of ICH Q2 (R1) guidelines.

scholarly journals Quick Preparative Separation of Natural Naphthopyranones with Antioxidant Activity by High-Speed Counter-Current Chromatography

2002 ◽  
Vol 57 (11-12) ◽  
pp. 1051-1055 ◽  
Author(s):  
Gilda G. Leitão ◽  
Suzana G. Leitão ◽  
Wagner Vilegas
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
High Speed ◽  
Ethanolic Extract ◽  
Solvent System ◽  
Spectrophotometric Assay ◽  
Preparative Separation ◽  
Dpph Radical ◽  
Preparative Scale ◽  
Counter Current

The natural naphthopyranones paepalantine (1), paepalantine-9O-β-ᴅ-glucopyranoside (2) and paepalantine-9-O-β-ᴅ-allopyranosyl-(1→6)-O-β-ᴅ-glucopyranoside (3) were separated in a preparative scale from the ethanolic extract of the capitula of Paepalanthus bromelioides by high-speed counter-current chromatography (HSCCC). The solvent system used was composed of water-ethanol-ethyl acetate-hexane (10:4 : 10:4, v/v/v/v). This technique led to the separation of the three different naphthopyranone glycosides in pure form in approximately 7 hours. Paepalantine showed a good antioxidant activity when assayed by the DPPH radical spectrophotometric assay.

scholarly journals Determination of Delafloxacin in Pharmaceutical Formulations Using a Green RP-HPTLC and NP-HPTLC Methods: A Comparative Study

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 359 ◽  
Author(s):  
Prawez Alam ◽  
Essam Ezzeldin ◽  
Muzaffar Iqbal ◽  
Gamal A.E. Mostafa ◽  
Md. Khalid Anwer ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Silica Gel ◽  
Solid Lipid Nanoparticles ◽  
Ternary Mixture ◽  
Mobile Phase ◽  
Pharmaceutical Formulations ◽  
Lipid Nanoparticles ◽  
Ammonia Solution ◽  
Hptlc Method

In this work; delafloxacin (DLFX) was determined using a validated green RP-HPTLC and NP-HPTLC methods in commercial tablets and in-house developed solid lipid nanoparticles (SLNs). RP-HPTLC determination of DLFX was performed using “RP-18 silica gel 60 F254S HPTLC plates”. However; NP-HPTLC estimation of DLFX was performed using “silica gel 60 F254S HPTLC plates”. For a green RP-HPTLC method; the ternary combination of ethanol:water:ammonia solution (5:4:2 v/v/v) was used as green mobile phase. However; for NP-HPTLC method; the ternary mixture of ethyl acetate: methanol: ammonia solution (5:4:2 v/v/v) was used as normal mobile phase. The analysis of DLFX was conducted in absorbance/reflectance mode of densitometry at λmax = 295 nm for both methods. RP-HPTLC method was found more accurate, precise, robust and sensitive for the analysis of DLFX compared with the NP-HPTLC method. The % assay of DLFX in commercial tablets and in-house developed SLNs was determined as 98.2 and 101.0%, respectively, using the green RP-HPTLC technique, however; the % assay of DLFX in commercial tablets and in-house developed SLNs was found to be 94.4 and 95.0%, respectively, using the NP-HPTLC method. Overall, the green RP-HPTLC method was found superior over the NP-HPTLC. Therefore, the proposed green RP-HPTLC method can be successfully applied for analysis of DLFX in commercial tablets, SLNs and other formulations containing DLFX.

Topical Wound Healing, Antimicrobial and Antioxidant Potential of Mimosa pudica Linn root Extracted using n-Hexane Followed by Methanol, Fortified in Ointment Base

2021 ◽  
Vol 14 (3) ◽  
pp. 5472-5480
Author(s):  
Sangeeta Singh ◽  
Tanvi R Dodiya ◽  
Sudarshan Singh ◽  
Rajesh Dodiya
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Solvent System ◽  
Perennial Herb ◽  
Maximum Effect ◽  
Root Extract ◽  
Mimosa Pudica ◽  
Ointment Base

Mimosa pudica Linn is an ornamental perennial herb belonging to the taxonomic group of Magnoliopsida and family Leguminaceae. Besides its ornamental use, Mimosa pudica is also a popular plant among folk healers to treat several diseases including bleeding disorders such as menorrhagia, dysentry with blood, mucus and piles, and wound healing. In this study, roots of Mimosa pudica was successively extracted with hexane, ethyl acetate, and methanol to evaluate the folkloric assert of plant in view of antioxidant, antibacterial, and wound healing potential. Methanolic extract showed concentration dependent elevation in inhibition for radical scavenging assay following dpph with maximum effect of 73.43% against standard ascorbic acid equivalent to 250 µg/ml, however IC50 of extract with n-hexane and ethyl acetate exhibited relatively poor antioxidant efficacy. Minimum inhibitory concentration demonstrated higher inhibitory action in range of 1.25-0.625 mg/ml against tested gram positive Staphylococcus aureus -MTCC737, and Bacillus subtilis-MTCC110 and 2.5 mg/ml for gram negative Salmonella typhi-MTCC15442, Escherichia coli-MTCC118 micro-organism for methanolic extract compared to ethyl acetate extract, however hexane extract showed MIC > 2.5 mg/ml. Among the tested solvent system for column chromatography, fractionation with diethyl ether: methanol (1:4) demonstrated single clear spot with Rf value 0.72 for methanolic extract indicated presence of flavonoid as major phytoconstituents. The results of spectral analysis following fourier transform spectroscopy, nuclear magnetic resonance, and mass spectroscopy for purified isolated compound confirmed identity of isolated flavonoid from methanolic fraction with chemical name 7,8,3’,4’-tetrahydroxy-6-methoxy-flavone. Thus, overall results demonstrated that Mimosa pudica root extract have potential antioxidant and antimicrobial efficacy. Furthermore, the Mimosa pudica root extracted with n-hexane followed by methanol fortified in a pharmaceutical formulation ointment base demonstrated excellent wound healing efficacy compared to marketed povidone-iodine ointment. Therefore, traditional claim for the use of Mimosa pudica root as potential antioxidant, antimicrobial, and wound healer in topical drug delivery have been proven by the present study. 

scholarly journals Influence of Different Chromatographic Conditions on the Level of Detection and Quantitation of Spironolactone by means of TLC-Densitometry

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Małgorzata Dołowy
Keyword(s):  
Quality Control ◽  
Acetic Acid ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
Glacial Acetic Acid ◽  
Cost Effective ◽  
Pharmaceutical Formulations ◽  
The One ◽  
Tlc Analysis ◽  
Routine Quality Control

The aim of this study was to estimate the influence of different chromatographic conditions on the limits of detection and limits of quantitation (LODs and LOQs) of spironolactone by means of TLC-densitometry under different chromatographic conditions. A comparison of results obtained showed that the choice of appropriate chromatographic conditions for NP-TLC and RP-TLC analysis with densitometry could effectively decrease the LODs and LOQs of spironolactone. Of all chromatographic systems tested, the best was the one comprising chromatographic plates precoated with a mixture of silica gel 60, kieselguhr F254, and mobile phase A (n-hexane-ethyl acetate-glacial acetic acid, 24.5 : 24.5 : 1, v/v/v). The estimated average LOD and LOQ values were 0.034 and 0.103 μg/spot, respectively. This indicates that the described procedure is sufficiently sensitive for the identification and quantification of spironolactone alone. Thereby, the simple and cost-effective TLC-densitometric method can be utilized for the routine quality control of spironolactone in bulk drugs as well as in simple pharmaceutical formulations.

scholarly journals Preparative Separation of Three Monoterpenes from Perilla frutescens var. crispa Using Centrifugal Partition Chromatography

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Bomi Nam ◽  
Sunil Babu Paudel ◽  
Jin-Baek Kim ◽  
Chang Hyun Jin ◽  
Dongho Lee ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Mobile Phase ◽  
Solvent System ◽  
Perilla Frutescens ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Drying Process ◽  
Two Phase ◽  
C Nmr

Three monoterpenes, namely, 9-hydroxy isoegomaketone (1), isoegomaketone (2), and perilla ketone (3), were successfully separated from the supercritical carbon dioxide (SC-CO2) extract of the leaves of Perilla frutescens var. crispa (cv. Antisperill; Lamiaceae) by centrifugal partition chromatography (CPC). To obtain large quantities of these materials required for studies on their mechanism of action and in vivo effectiveness in inflammation, we used CPC because of its high loading capacity and reproducibility to purify the three compounds. Compound 1 (2.60 mg, 96.7% purity at 254 nm) was purified from 500 mg of the SC-CO2 extract of P. frutescens var. crispa (cv. Antisperill), using a two-phase solvent system comprising n-hexane/ethyl acetate/ethanol/water (5:5:5:5 v/v) in a descending mode. As compounds 2 (56.1 mg, 97.6% purity at 254 nm) and 3 (78.6 mg, 96.1% purity at 254 nm) are highly volatile and difficult to recover from an aqueous mobile phase after purification during the drying process, they were obtained from the same amount of the processed extract in an ascending mode using the upper organic phase as the mobile phase (n-hexane/ethyl acetate/ethanol/water, 8:2:8:2 v/v). The structures of compounds 1–3 were confirmed by 1H- and 13C-NMR analysis. Thus, based on our findings, we recommend centrifugal partition chromatography as a powerful technique for purifying the active principal compounds 1 and 2 from the leaves of P. frutescens var. crispa.

scholarly journals Utilization of UPLC-tandem mass spectrometry for quantitation of colouring matters in dietary formulation

2019 ◽  
Vol 10 (3) ◽  
pp. 239-243
Author(s):  
Wafaa Abdou Zaghary ◽  
Ahmed Mostafa Abdalla ◽  
Emily Tawfik Hanna ◽  
Marwa Abdel Rahman Tohamy Zanoun
Keyword(s):  
Mass Spectrometry ◽  
Formic Acid ◽  
Mobile Phase ◽  
Multiple Reaction Monitoring ◽  
Ultra Performance Liquid Chromatography ◽  
Tandem Mass ◽  
Reaction Monitoring ◽  
The Matrix ◽  
Tandem Mass Spectrometric ◽  
Routine Quality Control

A simple and sensitive ultra-performance liquid chromatography with Tandem Mass Spectrometric detection (UPLC-MS/MS) method was developed and validated for the simultaneous quantitation of two food colouring matters, Curcumin (CUR) and Riboflavin (RIB). Chromatographic separation was done on Hypersil gold 50×2.1 mm (1.9 μm) column, with gradient programing of mobile phase starting with aqueous 0.1% formic acid and increasing the percentage of 0.1% formic acid in acetonitrile. The utilization of multiple reaction monitoring (MRM) improved the selectivity of detection and decreased the matrix effect.  The method was linear in the range of 5-500 ng/mL for CUR and RIB. Intra- and inter-day reproducibility were within the accepted criteria. The method was successfully applied for the determination the laboratory prepared mixtures of the two selected colouring matters. The use of mass spectrometry enhanced the selectivity and sensitivity of detection which allows the robust use of the method in routine quality control tests of the two colouring matters (CUR and RIB).

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