Assessment of Zinc, Iron, and Microbes concentrations in Blood Cockles (Anadara granosa) as Complementary Foods and Implications for reducing of Micronutrition Deficiency

2021 ◽  
pp. 3399-3403
Author(s):  
Margaretha Solang ◽  
Djuna Lamondo ◽  
Ramli Utina ◽  
Syam Kumaji ◽  
Zuliyanto Zakaria
Keyword(s):  
Heavy Metals ◽  
Plate Count ◽  
Food Control ◽  
National Agency ◽  
Filter Feeder ◽  
Total Plate Count ◽  
Zn Content ◽  
Blood Cockle ◽  
Anadara Granosa

Blood cockles (Anadara granosa) are filter feeder that can accumulate heavy metals and microbes so that need to pay attention to the maximum intake. The objective of this study was to evaluate the content of Fe, Zn, a total of microbes and maximum tolerable intake value (MTI). The study employed quantitative analysis. The samples were collected from Wonggarasi Timur Village, Pohuwato Regency, Gorontalo, Indonesia. The determination of Fe and Zn was carried out through Atomic Absorption Spectrophotometer and the microbes total was done by using the Total Plate Count test. Maximum Tolerable Intake value was calculated according to the provisions of JEFCA. The results of the study showed that the Fe and Zn average of blood cockles ranged from 227.85±5.04 ppm – 247.98±6.77ppm and 2.77±0.07ppm-2.82±0.09ppm. Microbes total ranged from 1.7 x 103 colonies/g – 2.4 x 103 colonies/g. The MTI value assumed for person weighing 70 kg based on Fe and Zn content of blood cockle is 0.226kg/day - 0.467kg/day and 24.822kg/day - 24.911kg/day. Blood cockles are a source of Fe, Zn and are safe for consumption according to MTI. Consumption of blood cockles helps reduce the problem of deficiency of Fe and Zn. The content of Fe is higher than the content of Zn in blood cockles. Blood cockles from Pohuwato regency are safe for consumption according to MTI. The microbes total in blood cockles ranged from 1.7x103 colonies/g -2, 4x 103 colonies/g. This total is below the Badan Pengawas Obat dan Makanan/ Indonesian National Agency of Drug and Food Control (BPOM) standard, which is 5 x 105 CFU/g so that the blood cockles are safe for consumption.

scholarly journals Analysis of Total Plate Count, Yeast and Mould Count, Salmonella sp, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Shigella spp. on Traditional Medicines

2019 ◽  
Author(s):  
Anas Gilang Pratama ◽  
Ruth Elenora Kristanty ◽  
Silvester Maximus Tulandi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Raw Material ◽  
Plate Count ◽  
Food Control ◽  
National Agency ◽  
Traditional Medicines ◽  
Total Plate Count ◽  
Shigella Spp

Anas Gilang Pratama, “Analysis of Total Plate Count, Yeast, and Mould Count, Salmonella spp., Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Shigella spp. on Traditional Medicines” under the guidance of Silvester Maximus Tulandi, S.Farm., M.Sc., and Ruth Elenora Kristanty, M.Farm., Apt., 2019. Traditional medicines can be contaminated by bacteria starting from the raw material process, during production, and at the time of storage. The analysis of total plate count, yeast, and mould count on traditional medicines was done at the Microbiology Laboratory of National Agency of Drug and Food Control in Jakarta by using five types of bacteria. This work was based on microbiological test parameters on traditional medicines through several stages: homogenization, enrichment, isolation, and biochemical tests. Based on the results, the samples fulfilled the requirements on Analysis of Total Plate Count, Yeast, and Mould Count, Salmonella sp, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Shigella spp. according to the Regulation of the Head of the National Agency of Drug and Food Control No.12/2014.

scholarly journals Heavy Metals Content in Phaeophyceae from Malang Rapat Waters, Bintan

2021 ◽  
Vol 26 (1) ◽  
pp. 57-62
Author(s):  
Hilda Novianty ◽  
Emienour Muzalina Mustafa ◽  
Suratno Suratno
Keyword(s):  
Heavy Metals ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Dry Weight ◽  
Raw Material ◽  
National Agency ◽  
Brown Macroalgae ◽  
Turbinaria Conoides ◽  
Processed Products

The use of brown-macroalgae (Phaeophyceae) as an alginate source is very broad, covering the food and non-food industry, because of that it is necessary to know the safety of these natural resources, one of which is safe from heavy metal contamination. The purpose of this study was to determine heavy metals concentrations accumulated in several types of Phaeophyceae (brown macroalgae) as an alginate source from Malang Rapat waters, Bintan. The method used was descriptive non-experimental. The determination of the location of sampling was done by purposive sampling method. Samples were taken through free collection, identified species and measured of metal contamination concentrations for As, Cd, and Pb. The data obtained were analyzed descriptively. The results showed that Phaeophyceae found in Malang Rapat waters were Turbinaria conoides, Sargassum aquifolium, Padina australis, Hormophysa cuneiformis with each metal concentration contamination of As 13.95 to 23.30 µg.g-1 dry weight; Cd from 0.33 to 1.08 µg.g-1 dry weight and Pb from 4.72 to 9.92 µg.g-1 dry weight. This study showed that all metal contamination ranges in all Phaeophyceae were on the verge of the maximum limit set by the National Standardization Agency of Indonesia Indonesian (SNI) No 7387 of 2009 and National Agency of Drug and Food Control of RI (BPOM) Regulation No 5 of 2018 for dried macroalgae product so that Phaeophyceae from Malang Rapat waters - Bintan was not safe to be used as raw material for alginate source or other processed products of brown-macroalgae.

scholarly journals HEAVY METAL CONTENT IN SOME SEAFOODS COLLECTED FROM MUARA ANGKE FISH AUCTION PLACE, JAKARTA.

1987 ◽  
Vol 26 ◽  
pp. 51-58
Author(s):  
Horas Hutagalung ◽  
Suwirma Syamsu
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Metal Content ◽  
Heavy Metal Content ◽  
World Health ◽  
Human Consumption ◽  
Zn Content ◽  
Standard Set ◽  
Health Organization

Determination of  heavy  metal  content  (Hg, Pb, Cd, Cu, Cr, Zn) in some seafoods collected from  Muara  Angke  Fish  Auction  Place  were carried out during February to July 1979. The results showed  that Zn  content  in seafoods a nalyzed  was always much higher than the other heavy metals. The concentration levels of  the  six  heavy  metals observed were low. It is almost of the same level as the metal concentration of  other  seafoods  observed  in various parts of the world oceans, but still lower  than the maximum standard set  for human  consumption  as  established  by   Tasmania, Australia and  World Health Organization. The  mean  per capita  consumption of  fish  in Jakarta is only  about 165 g  per week, while  the  Provisional Tolerated Weekly Intake for Hg, set by WHO/FAO at 300 ug level, would be attained by consuming 1500 g Rastrelliger sp., or 698 gPenaeus setiferus, or 545 g Sepia sp., and or 1250 g Pecten sp.  Therefore,  at   present the  level of metal content in fishes sold at TPI  Muara  Angke  can be  considered  as  not  dangerous  to human health as long as these quantities are not surpassed.

Proximate, microbial and mineral compositions of Millet Pennisetum gluacuum L. and sorghum bicolor compounded straw feed for ruminant

2021 ◽  
Vol 3 (2) ◽  
pp. 009-014
Author(s):  
Bello Muhammed magaji ◽  
Femi Maroof Adams ◽  
Azeez Ayodeji Tijani ◽  
Bamidele Joshua Awogbemi ◽  
Temitope Oluwaranti Alake ◽  
...  
Keyword(s):  
Sorghum Bicolor ◽  
Crude Protein ◽  
Plate Count ◽  
Microbiological Analysis ◽  
Crude Fibre ◽  
Total Plate Count ◽  
Mineral Compositions ◽  
Digestible Protein ◽  
Ruminant Feed

The study was aimed for the production and determination of the proximate, microbiological and mineral compositions of Millet Pennisetum gluacuum L. and Sorghum bicolor compounded straw feed for ruminant production. The result revealed a pH 5.791 which is suitable for fibre in the feed and promote chewing and rumination processes in the rumen, low percentage moisture content of 1.37 was recorded due to high drying in the field, high percentage fat and crude protein of 4.67 and 6.97 respectively this was as a result of the inclusion of groundnut cake as source of protein and fat in the feed, percentage digestible protein was 3.58, crude fibre value of 27.56 which satisfied the 18% requirement in the diet of ruminants, percentage carbohydrate was 54.2, and estimated energy value (Kcal) 286.95. Microbiological analysis showed a total plate count of 5 x10-4 cfu/g, Mould count of 1 x10-3 cfu/g, while Yeast, Staphylococcus, Coliform, Shigella, Salmonella and Escherichia coli count were not detected respectively. Mineral composition of sorghum and millet Pennisetum gluacuum compounded straw feed showed the presence of Fe, Na, K, Mg, Zn, Cu Mn, and Cr as 120.4189mg/kg, 570.9844 mg/kg, 227.1493 mg/kg, 568.2669 mg/kg, 12.9195mg/kg, 14.0666mg/kg, 62.9926mg/kg respectively and 18.584 mg/kg while Ni and Pb were not detected in the formulated ruminant feed.

Microbiology of Delicatessen Salads

1975 ◽  
Vol 38 (3) ◽  
pp. 146-149 ◽  
Author(s):  
J. L. FOWLER ◽  
W. S. CLARK
Keyword(s):  
Air Force ◽  
The United States ◽  
Fecal Coliforms ◽  
Plate Count ◽  
Convenience Food ◽  
Total Plate Count ◽  
Health Significance ◽  
Yeasts And Molds ◽  
Geographical Locations

Prepared salads obtained from 11 convenience food manufacturers in eight geographical locations throughout the United States were examined microbiologically upon their arrival at the laboratory, stored at 2 C and examined microbiologically weekly thereafter for 5 weeks. Microbiological evaluation included determination of total plate count, coliforms, fecal coliforms, yeasts and molds, Clostridium perfringens, Staphylococcus aureus. and salmonella; pH of the salads also was measured. Microorganisms of public health significance were detected initially in few samples; during storage these microorganisms decreased in number and no longer could be detected. Yeasts and molds, and microorganisms enumerated by the total plate count consistently proliferated to extremely high numbers in shrimp, macaroni, carrot and raisin, and egg salads. Additionally, 64 prepared salads representing 13 convenience food manufacturers from 10 geographic locations were examined microbiologically for compliance with Army and Air Force Exchange Service microbiological limits. Counts in excess of the limits were found in 36 of 64 (56%) samples; violations in total plate count, coliform, and yeast and mold limits occurred in 16, 22, and 45% of the samples, respectively.

scholarly journals Pengaruh Lama Pemakaian Sediaan Kosmetik Bedak Padat Terhadap Cemaran Mikroba

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Munira Munira ◽  
Cut Fardilla ◽  
Noni Zakiah ◽  
Rasidah Rasidah ◽  
Muhammad Nasir
Keyword(s):  
Bacterial Contamination ◽  
Plate Count ◽  
Test Results ◽  
Food Control ◽  
Average Value ◽  
Total Plate Count ◽  
Compact Powder ◽  
Long Time ◽  
The Republic ◽  
Number Of Bacteria

Bedak adalah salah satu sediaan kosmetik yang dipakai dalam jangka waktu yang lama. Bedak yang digunakan akan terjadi kontaminasi bahkan semakin meningkat seiring dengan lamanya pemakaian. Faktor-faktor yang mempengaruhi antara lain penyimpanan, lingkungan, dan spons bedak. Penggunaan spons bedak merupakan salah satu sumber cemaran bakteri. Di mana penggunaan spons yang sama secara berulang-ulang dan bersentuhan langsung pada kulit dapat meningkatkan jumlah mikroba. Tujuan penelitian ini adalah untuk mengetahui pengaruh lamanya penyimpanan, pemakaian suatu sediaan kosmetik bedak padat terhadap tingkat cemaran mikroba. Sampel yang diuji adalah bedak padat yang belum dipakai dan yang telah dipakai selama 2 minggu. Hasil penelitian berupa jumlah cemaran bakteri (ALT) dibandingkan dengan syarat menurut Peraturan Kepala Pengawasan Obat dan Makanan RI No.HK.03.1.23.07.11.6662 Tahun 2011 yaitu tidak boleh melebihi 103 koloni/g. Hasil uji cemaran bakteripada sediaan bedak padat sebelum pemakaian mempunyai nilai rata-rata ALT 2,6 x 101koloni/g dan sesudah pemakaian mempunyai nilai rata-rata ALT 9,2 x 101 koloni/g. Nilai ALT yang diperoleh dari masing-masing sediaan kosmetik bedak padat telah memenuhi syarat.Hasil uji t dependen menunjukkan adanya perbedaan jumlah bakteri pada bedak padat sebelum dipakai dengan setelah dipakai selama 2 minggu (t = 0.000). Kata Kunci: Kosmetik, bedak padat, Angka Lempeng Total ABSTRACTPowder is one of the cosmetic preparations used for a long time. Powder that is used will occur contamination even increasing along with the length of usage. This can be caused by several factors including storage, environment, powder sponges and others. The use of powder sponges is one source of bacterial contamination. Where the use of the same sponge repeatedly and in direct contact with the skin can increase the number of microbes. The objective of study was to determine the effect of the duration of use of a compact powder cosmetic preparation on the level of microbial contamination. The samples tested were solid powder that has not been used and that has been used for 2 weeks. The results of the study were the Total Plate Count (TPC) compared to the Regulation of the Head of the Republic of Indonesia Drug and Food Control Number HK.03.1.23.07.11.6662 of 2011 concerning the Requirements for Microbial Pollution and Heavy Metals in Cosmetics which should not exceed 103 colonies / g. The test results of bacterial contamination on solid powder preparations before use have an average value of ALT 2,6 x 101 colony / g and after use have an average ALT value of 9.2 x 101 colony / g. The ALT value obtained from each compact powder cosmetic preparation has met the requirements. The results of the dependent t test showed a difference in the number of bacteria on solid powder before use with after being used for 2 weeks (t = 0.000). Keywords: Cosmetics, Compact Powder, Total Plate Count

scholarly journals Studi Kandungan Logam Berat Merkuri (Hg) dan Prediksi Kandungan Metil Merkuri (CH3Hg) pada Organ Kerang Darah (Anadara granosa) di Kecamatan Sidayu dan Kecamatan Banyuurip, Pantai Utara Gresik, Jawa Timur [Study of Heavy Metal Content of Mercury (Hg) and Prediction Content of Methyl Mercury (CH3Hg) on The Blood Shellfish’ (Anadara granosa) Organs In Sidayu and Banyu Urip District, Gresik, East Java]

2019 ◽  
Vol 6 (2) ◽  
pp. 207
Author(s):  
Baskara Adam Saleh, Boedi Setya Rahardja, Muhammad Arief
Keyword(s):  
Heavy Metals ◽  
Coastal Area ◽  
Methyl Mercury ◽  
Industrial Effluent ◽  
River Estuary ◽  
Mercury Contamination ◽  
Filter Feeder ◽  
Average Value ◽  
Blood Clam ◽  
Anadara Granosa

Abstract Oil drilling and industrial effluent that wasted through the Bengawan Solo River estuary is a source of environmental pollution in the Sidayu and Banyu Urip district, Gresik as one that produces the blood shellfish (Anadara granosa). One of the most dangerous contaminants of heavy metals is mercury. However shellfish can accumulate more heavy metals than other aquatic animals because it settle and filter their food (filter feeder) and slow to be able to avoid the effects of pollution The Aim of this study are to determine the levels of mercury and methyl mercury predictions on blood shellfish (Anadara granosa). The process of sampling was done at coastal area of Sidayu and Banyu Urip district, Gresik then performed using Atomic Absorbance readings Spectrophotometric (AAS). This is a descriptive study and the data obtained, and then continued with stoichiometric calculations to predict the content of methyl mercury. Based on the analysis of mercury and prediction of methyl mercury in the blood shellfish (Anadara granosa) organs in coastal area of Sidayu and Banyu Urip district, Gresik, has not exceeded the threshold of SNI, the value is 0.5 ppm. Mercury contamination was high in blood clam shell with the average value of 0.273 ppm and 0.293 ppm, followed by the stomach and intestines shell with an average value of 0.226 ppm and 0.281 ppm. Lowest contamination is on the organ gill, liver, and kidneys with the average value of 0.150 ppm and 0.133 ppm.

scholarly journals ANALYSIS OF HEAVY METAL CONTENT IN Anandara granosa (Linnaeus, 1758): A CASE STUDY OF 10 MARKETS IN SEMARANG, CENTRAL JAVA, INDONESIA

2016 ◽  
Vol 78 (4-2) ◽  
Author(s):  
Nanik Heru Suprapti ◽  
Aziz Nur Bambang ◽  
Fronthea Swastawati ◽  
Ahmad Ni’matullah Al Baari ◽  
Adriyan Pramono
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Heavy Metal Contamination ◽  
Tea Leaves ◽  
Citrus Aurantifolia ◽  
Central Java ◽  
Micronutrient Status ◽  
Significant Difference ◽  
Blood Cockle ◽  
Anadara Granosa

The purpose of this study was to evaluate Pb, Cr, and Cd levels inside the Anadara granosa and to investigate the effect of submersion using Citrus aurantifolia (lime) and Orthosiphon aristatus (kidney tea leaves) solution on Pb, Cr, and Cd levels contained in the blood cockles taken from 10 markets in the city of Semarang, Central Java, Indonesia (Johar, Genuk, Gayamsari, Jati, Peterongan, Karangayu, Mangkang, Pedurungan, Boom Lama, and Ngaliyan). The concentration of heavy metals of Anadara granosa was analyzed using Atomic Absorption Spectrometer (AAS). Analysis of variance (ANOVA) showed that heavy metals (Pb, Cd, and Cr) of blood cockle taken from 10 markets provided no significant difference (P < 0.05) on the content of Pb, Cd and Cr. Purification was carried out using Citrus aurantifolia (lime) and Orthosiphon aristatus (kidney tea leaves) solution. The lowest Pb (0.00 mg kg–1) was obtained from Boom Lama and Peterongan market with purification using kidney tea leaves solution, while the lowest Cr [(0.30 ± 0.00) mg kg–1]) was obtained from Genuk market with lime solution. In addition, the lowest Cd (0.00 mg kg–1) was obtained from Boom Lama market with purification using kidney tea leaves solution. Accumulation of heavy metal contamination in blood cockle could affect the micronutrient status and consumer health.

scholarly journals Concentration of Heavy Metals (Pb and Cu) in Alepes vari and Johnius belangerii from Musi River Estuary, Banyuasin District, South Sumatra

2019 ◽  
Vol 8 (2) ◽  
pp. 213-219
Author(s):  
Fitri Agustriani ◽  
Anna Ida Sunaryo Purwiyanto ◽  
Wike Ayu Eka Putri ◽  
Fauziyah Fauziyah
Keyword(s):  
Heavy Metals ◽  
Atomic Absorption ◽  
High Activity ◽  
Negative Impact ◽  
River Estuary ◽  
Atomic Absorption Spectrophotometer ◽  
Food Control ◽  
National Agency ◽  
Ecosystem Changes ◽  
Musi River

Agustriani F, Purwiyanto AIS, Putri WAE, Fauziyah. 2019. Concentration of heavy metals (Pb and Cu) in alepes vari and johnius belangerii from musi river estuary, Banyuasin district, South Sumatra. Jurnal Lahan Suboptimal: Journal of Suboptimal Lands. 8(2):213-219. The Musi river estuary plays an important role as one of the fishing grounds. The high activity in the musi estuary has an impact on the ecosystems in this region, including the negative impact in the form of ecosystem changes caused by heavy metals. The aim of this study was to determine the concentrations of heavy metals (Pb and Cu) in Alepesvariand Johniusbelangerii in the Musi river estuary. Fish were obtained from fishermen who used the Gillnet Millennium (Ply) gear in July 2018 and were analyzed using the Atomic Absorption Spectrophotometer (AAS). The results showed that the concentration of  Pb  in J. belangerii was greater than A. vari, but the concentration of Cu  in  A. vari  was greater than in  J. belangerii. Concentration of  Pb has exceeded the limit for safe consumption based on National Agency of Drug and Food Control.

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