Phylogenetic of Marble Goby (Oxyeleotris marmorata Blkr.) in South Sumatra Based on Cytochrome C Oxidase Subunit I (COI) Gene

Marble goby (Oxyeleotris marmorata) in Indonesia, spread across Sumatra, Kalimantan and Papua. The purpose of this study was to utilize a sequence of mitochondrial DNA Cytochrome Oxidase Subunit I gene, to analyze the phylogenetic tree and genetic distance between cultured and captured populations. This research was conducted on March-August 2020. The methods used in barcoding species were DNA isolation, amplification using PCR (Polymerase Chain Reaction) and sequencing of the COI mtDNA gene. The domesticated samples (n=3) were collected from Gandus Fish Seed Center (GFSC), while the wild samples (n=3) were captured from the Musi River in Beruge Village, Babat Toman District, both in Musi Banyuasin Regency. The sequenced COI mtDNA gene fragments were obtained from the PCR method. Three samples performed good PCR results, while the other three didn’t amplify properly. After the editing process, the COI gene sequencing produced a nucleotide length of 610 bp. Based on the BLAST (Basic Local Alignment Search Tool), domesticated marble goby (OMD2, OMD3) was in the same cluster with marble goby from the Musi River (OMS3). The genetic distance indicated that two specimens of domesticated marble goby were 100% identical, while the wild (OMS3) indicated a genetic distance of 0.0066 to domesticated species.

Short Communication: Investigating gonad length and fecundity in mudskippers (Gobiidae) at the Musi River estuary, South Sumatra, Indonesia

Ridho MR, Patriono E, Wati DR, Pratama R, Avesena M. 2021. Short Communication: Investigating gonad length and fecundity in mudskippers (Gobiidae) at the Musi River estuary, South Sumatra, Indonesia. Biodiversitas 22: 4413-4420. Information on the biological aspects of mudskipper (Gobiidae) reproduction is needed as a conservation effort to use economically valuable and sustainable resources in the future. This can be used to enrich the information on the status of the mudskipper research at the Musi River estuary, South Sumatra, Indonesia. The field sampling was carried out by using the purposive sampling method. The sampling of fish was carried out by the researchers and assisted by the people of the community using fishing rods and bubu fish traps. The results showed that 122 mudskipper were obtained, which consisted of four species Boleophthalmus boddarti, Boleophthalmus dussumieri, Periophthalmus chrysospilos, Periophthalmodon schlosseri. 93 male and 29 female fish were caught, the sex ratio was 3.20:1. There are more male than female fish overall with the sampling method used. Most of the female fish fecundity was reported in P. chrysospilos, namely 23,270-36,264 items in GM (III) and ranged from 40,825-87,749 items in GM (IV). The largest egg diameter was reported in P. chrysospilos by 0.051-0.069 mm (GML III) and 0.053-0.062 mm (GML IV), while the smallest weight and length were reported in P. chrysospilos than other species. The egg size was not positively correlated with body size.

PENGEMBANGAN LKPD MATERI HIMPUNAN KONTEKS PENCARIAN HARTA KARUN DI SUNGAI MUSI

ABSTRAKSumber belajar yang dapat dikembangkan untuk membantu peserta didik menjadi aktif dan memahami materi salah satunya adalah LKPD. LKPD adalah bahan ajar berupa lembaran didesain berisi latihan berisi langkah-langkah tugas yang harus diselesaikan peserta didik. Rumusan masalah dalam penelitian ini adalah bagaimana mengembangkan LKPD materi himpunan konteks pencarian harta karun di Sungai Musi yang valid dan praktis. Metode penelitian yang digunakan adalah pengembangan dengan dua tahap yaitu preliminary study dan formatif study. Penelitian ini hanya pada tahap self evaluation, expert review, one to one, dan small group dengan teknik pengumpulan data menggunakan walk through, dokumentasi, wawancara, dan angket. Penelitian ini menghasilkan produk LKPD materi himpunan konteks pencarian harta karun di Sungai Musi dengan kategori valid dan praktis. Kata kunci :Himpunan, LKPD, Konteks ABSTRACTOne of the learning resources that can be developed to help students become active and understand the material is LKPD. LKPD is a teaching material in the form of a sheet designed to contain exercises containing the steps of the task that must be completed by students. The formulation of the problem in this research is how to develop valid and practical LKPD for the context of finding treasures in the Musi River. The research method used is development with two stages, namely preliminary study and formative study. This research is only at the stage of self evaluation, expert review, one to one, and small group with data collection techniques using walk through, documentation, interviews, and questionnaires. This research produces LKPD products for the context of finding treasures in the Musi River with valid and practical categories. Keywords :Set, LKPD, Context

The Meaning of Water Environment for Stilt Houses on the Musi Riverbanks, Palembang

Water functions are very diverse for humans for daily needs such as drinking water, bathing, agriculture, development, aesthetics, and lower temperatures. Palembang is a lowland area dominated by wetlands, tidal swamps, and rivers. The types of houses on stilts on the banks of the Musi river are limas, Gudang, and Chinese stilt houses which are always associated with the water environment. The construction of roads replacing the role of rivers has realized people that the water environment has an important meaning for the house on stilts. The research objective was to explore the meaning of the water environment from various aspects for residents, stilt houses, and their environment. This research method is a case study with data collection through interviews, observation, measurements, and taking photos. Data and findings were analyzed and juxtaposed with images in the field. The road construction has eliminated part of the function of the river and influenced the change in orientation of the house on stilts. In conclusion, respect for the water environment including rivers has decreased, causing the quality of the river to decline, and the house on stilts cannot take advantage of the existence of the water environment optimally

Polisiklik Aromatik Hidrokarbon (PAH) Di Sekitar Muara Sungai Musi Sumatera Selatan

Muara Sungai Musi dan Pulau Payung adalah kawasan bagian hilir Sungai Musi yang menerima banyak masukan limbah dan bahan pencemar akibat aktifitas di sepanjang aliran sungai. Polisiklik aromatik hidrokarbon (PAH) adalah salah satu komponen pencemar organik yang keberadaannya mengancam kehidupan biota perairan. Penelitian ini bertujuan untuk mengidentifikasi keberadaan senyawa PAH pada sampel air laut dan sedimen yang berasal dari Muara Sungai Musi dan sekitar Pulau Payung. Pengambilan sampel dilakukan pada bulan Juli 2019. Contoh air laut diambil dengan water sampler pada 5 stasiun penelitian dan contoh sedimen diambil menggunakan ekman grab pada 8 stasiun penelitian. Kadar PAH dianalisa dengan High Performance Liquid Chromatography (HPLC), cara kerja merujuk pada APHA (2017) 6440 B divalidasi. Hasil penelitian menunjukkan bahwa kandungan PAH total dalam sampel air Muara Sungai Musi dan sekitar Pulau Payung berkisar antara batas minimum deteksi alat (<0,004)-0,0,62 ppb. Adapun kandungan PAH total dalam sampel sedimen berkisar antara 11,92-558,41 ppb. Secara keseluruhan terlihat bahwa kandungan PAH dalam sampel air dan sedimen yang berasal dari Sungai Musi dan sekitar Pulau Payung masih aman bagi organisme. Musi River Estuary and Payung Island are the downstream areas of the Musi River which receive a lot of inputs of waste and pollutants due to activities along the river flow. Polycyclic aromatic hydrocarbons (PAHs) is one component of organic pollutants which dangerous for the aquatic organism. This study aims to identify the PAHs compounds in seawater and sediment samples from the Musi River Estuary and around Payung Island. Sampling was carried out in July 2019. Seawater samples were taken with a water sampler and sediment samples were taken using ekman grab at 7 research stations. PAH levels were analyzed by High Performance Liquid Chromatography (HPLC), the method refers to APHA (2017) 6440 B. The results showed that the PAH content in the Muara River Musi water samples and around Payung Island ranged from under detection limit(<0,004)-0.62 ppb. The PAH content in sediment samples ranged from 11.92-558.41 ppb. Overall PAHs content in water and sediment samples from the Musi River and around Payung Island are still good for the aquatic organism.

Keanekaragaman Larva Ikan di Sekitar Muara Sungai Musi, Sumatra Selatan

Abstrak Estuari adalah salah satu habitat penting bagi ikan, namun terancam akibat peningkatan aktivitas manusia. Hal tersebut dapat memengaruhi keanekaragaman ikan di sekitarnya. Tujuan penelitian ini untuk menganalisis keanekaragaman dan kelimpahan larva ikan di sekitar muara Sungai Musi Sumatra Selatan. Penelitian ini menggunakan metode survei dengan cruise track design dan lintasan survei continuous parallel pada setiap stasiun secara swept area. Hasil penelitian menunjukkan bahwa indeks keanekaragaman (H’) di muara Sungai Musi pada Maret 2019 sebesar 0,639 sedangkan pada Mei 2019 sebesar 1,253. Kelimpahan relatif pada Maret 2019 lebih tinggi daripada Mei 2019. Kelimpahan Relatif (Kr) pada Maret 2019 antara 0,69–84,61% dan pada Mei 2019 antara 0,21–58,77%. Kelimpahan larva ikan yang ditemukan pada Mei lebih tinggi, yaitu sebanyak 456 individu dari 10 famili. Pada Maret ditemukan lebih rendah, yaitu sebanyak 143 individu dari 6 famili. Ditemukan larva ikan dari famili Engraulidae, Belonidae, Osphronemidae, Nemipteridae, Gerreidae, dan Gobiidae pada Maret. Sedangkan pada Mei 2019, yaitu Nemipteridae, Engraulidae, Scatophagidae, Chandidae, Lobotidae, Terapoinidae, Belonidae, Osphronemidae, Chanidae, Clupeidae. Abstract Estuary is one of important habitat for fish, yet is threatened by an increase in human activities. Thus affecting the diversity of fish around it. This study aim to determine the diversity and abundance of fish larvae around the mouth of Musi River in South Sumatra. This study was conducted using survey method with Cruise Track Design and continuous parallel survey track by swept area at each station. The results showed that the diversity index (H ') at the mouth of the Musi River in March 2019 was 0.639 while in May 2019 was 1.253. Relative Abundance (Kr) in March 2019 between 0.69–84.61% and in May 2019 between 0.21–58.77%. Abundance of fish larvae in May was found higher at 456 individuals from 10 families. Whereas in March it was found to be lower as many as 143 individuals from 6 families. The relative abundance in March 2019 was lower than in May. Fish larvae from the Engraulidae, Belonidae, Osphronemidae, Nemipteridae, Gerreidae and Gobiidae families were found in March. Whereas in May 2019 were Nemipteridae, Engraulidae, Scatophagidae, Chandidae, Lobotidae, Terapoinidae, Belonidae, Osphronemidae, Chanidae, Clupeidae.

FITOPLANKTON SEBAGAI BIOINDIKATOR PENCEMARAN ORGANIK DI PERAIRAN SUNGAI MUSI BAGIAN HILIR SUMATERA SELATAN

The Musi River is the largest and longest river in South Sumatra. The development of population activities in the Musi River Basin can affect river water quality and can cause pollution. The high level of industrial activity and households along the Musi River causes a decrease in environmental quality in the Musi River Basin. Based on this, further research is needed to determine the extent of pollution in the Musi River Basin. The purpose of this study is to assess saprobitas along the Musi River Basin based on SI (Saprobic Index) and TSI (Tropical Saprobic Index) values, and knowing the level of water pollution using saprobitas water assessment. This study uses plankton as the main material, namely water samples and plankton samples. This study uses an exploratory design with survey methods, and the determination of sampling stations by purposive sampling method. The results showed abundance of phytoplankton in the waters of the Musi River in the range of 123 - 2581 cells / liter or an average of 1397 cells / liter, the waters of the Musi River can be categorized as Oligotrophic waters with several station points that can be categorized as Mesotrophic waters. The Saprobic index in the waters of the Musi River ranges from 0.63 - 1, classified in the phases of the microbial, namely β-Mesosaprobic, so that the waters of the Musi River are classified as mild or low.