scholarly journals Бактериофаги Pseudomonas syringae pv. syringae перспективные в подавлении развития бактериального рака плодовых

2021 ◽  
Author(s):  
Анна Самойлова ◽  
Keyword(s):  
Pseudomonas Syringae ◽  
Fire Blight ◽  
Bacterial Canker ◽  
Causative Agents

Five Pseudomonas syringae pv. syringae bacteriophages were isolated from the quince, apple and pear. After a detailed study, the isolated bacteriophages could be used for biocontrol of the bacterial canker patho-gen. One of the isolated phages was active against the causative agents of bacterial canker and fire blight.

Effect of a protectant copper application on Psa infection of kiwifruit trap plants

2017 ◽  
Vol 70 ◽  
pp. 310-314
Author(s):  
J.L. Tyson ◽  
S.J. Dobson ◽  
M.A. Manning
Keyword(s):  
New Zealand ◽  
Disease Control ◽  
Pseudomonas Syringae ◽  
Low Risk ◽  
Bacterial Canker ◽  
Complete Protection ◽  
Leaf Spots ◽  
Copper Compounds ◽  
Trap Plants

Pseudomonas syringae pv. actinidiae (Psa) causes bacterial canker of kiwifruit, which is an ongoing threat to New Zealand kiwifruit production. Disease control depends on orchard practices such as removal of visibly diseased material, pruning during low-risk periods, and the application of foliar bactericides. Although the use of copper compounds on Actinidia species (kiwifruit) can cause phytotoxicity, copper-based formulations remain a key component of Psa control in New Zealand. The effect of single copper applications on Psa infection of ‘Hort16A’ trap plants was studied over the Spring of 2014 (Sept—Nov). Psa leaf spots were observed at the beginning of October, appearing first on the untreated plants. Although the copper sprays did not achieve complete protection, particularly as the inoculum built up during November, the copper-sprayed plants always had less disease than the untreated plants.

BIOASSAY TO DETERMINE CHERRY ROOTSTOCK TOLERANCE TO `BACTERIAL CANKER' CAUSED BY PSEUDOMONAS SYRINGAE PV SYRINGAE

HortScience ◽  
1990 ◽  
Vol 25 (11) ◽  
pp. 1354F-1355
Author(s):  
Elzbieta Krzesinska ◽  
Anita Nina Miller
Keyword(s):  
Relative Humidity ◽  
Pseudomonas Syringae ◽  
Bacterial Suspension ◽  
Avirulent Strain ◽  
Bacterial Canker ◽  
Symptom Development ◽  
Virulent Strains ◽  
Callus Production ◽  
Root Stock ◽  
One Year

An excised twig assay was developed to evaluate cherry geno-types for their tolerance to Pseudomonas syringae pv. syringae. One-year-old wood was collected at monthly intervals from October until January of `Royal Ann', `Corum', and a number of cherry rootstock. The rootstock included; F/12–1 and Giessen (GI) and M × M selections. A 2-cm incision (“^”-shaped flap) was made on each twig. A 20-μl droplet of inoculum or water was placed onto each incision. The inoculum was prepared with one avirulent (K4) and three virulent strains (W4N54, AP2, B15) concentrations (105, 106, or 107 cfu). Inoculated twigs were placed in test tubes and incubated at 15C in high relative humidity for 3 weeks. After incubation, twigs were evaluated for gummosis production (0–3, 0 = no gummosis), incision browning (1–4, 1 = yellow pith), and callus production (0–1, 0 = no callus). The concentration of bacterial suspension had no effect on symptom development. No gummosis or browning was observed on twigs inoculated with water or the avirulent strain. Based on the gummosis and browning ratings, rootstock M × M 2, M × M 39, M × M 60, GI 148-1, GI 154-2, and GI 154-4 were found to be resistant to these three strains of P. syringae in this assay. Root-stock F 12-1, GI 169–15, GI 172–9, and GI 173-9 were found to be tolerant.

scholarly journals A Synergic Potential of Antimicrobial Peptides against Pseudomonas syringae pv. actinidiae

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1461
Author(s):  
Nuno Mariz-Ponte ◽  
Laura Regalado ◽  
Emil Gimranov ◽  
Natália Tassi ◽  
Luísa Moura ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Antimicrobial Peptides ◽  
Pseudomonas Syringae ◽  
Pathogenic Bacteria ◽  
High Efficiency ◽  
Bacterial Canker ◽  
Membrane Permeation ◽  
Flow Cytometry Analysis ◽  
Pathogenic Agent ◽  
Peptide Mixtures

Pseudomonas syringae pv. actinidiae (Psa) is the pathogenic agent responsible for the bacterial canker of kiwifruit (BCK) leading to major losses in kiwifruit productions. No effective treatments and measures have yet been found to control this disease. Despite antimicrobial peptides (AMPs) having been successfully used for the control of several pathogenic bacteria, few studies have focused on the use of AMPs against Psa. In this study, the potential of six AMPs (BP100, RW-BP100, CA-M, 3.1, D4E1, and Dhvar-5) to control Psa was investigated. The minimal inhibitory and bactericidal concentrations (MIC and MBC) were determined and membrane damaging capacity was evaluated by flow cytometry analysis. Among the tested AMPs, the higher inhibitory and bactericidal capacity was observed for BP100 and CA-M with MIC of 3.4 and 3.4–6.2 µM, respectively and MBC 3.4–10 µM for both. Flow cytometry assays suggested a faster membrane permeation for peptide 3.1, in comparison with the other AMPs studied. Peptide mixtures were also tested, disclosing the high efficiency of BP100:3.1 at low concentration to reduce Psa viability. These results highlight the potential interest of AMP mixtures against Psa, and 3.1 as an antimicrobial molecule that can improve other treatments in synergic action.

scholarly journals Essential Oils with Inhibitory Capacities on Pseudomonas syringae pv. actinidiae, the Causal Agent of Kiwifruit Bacterial Canker

2017 ◽  
Vol 12 (1) ◽  
pp. 16-26 ◽  
Author(s):  
Pucci Nicoletta ◽  
Orzali Laura ◽  
Modesti Vanessa ◽  
Lumia Valentina ◽  
Brunetti Angela ◽  
...  
Keyword(s):  
Essential Oils ◽  
Pseudomonas Syringae ◽  
Causal Agent ◽  
Bacterial Canker

scholarly journals Isolation of the Novel Phage PHB09 and Its Potential Use against the Plant Pathogen Pseudomonas syringae pv. actinidiae

Viruses ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2275
Author(s):  
Yanxi Liu ◽  
Mengjiao Liu ◽  
Ran Hu ◽  
Jun Bai ◽  
Xiaoqing He ◽  
...  
Keyword(s):  
Host Range ◽  
Pseudomonas Syringae ◽  
Management Strategies ◽  
Lytic Bacteriophage ◽  
Bacterial Canker ◽  
Isolation And Identification ◽  
Narrow Host Range ◽  
Development Of Resistance ◽  
Wide Range ◽  
Complete Genome Sequence Analysis

Bacteriophages are viruses that specifically infect target bacteria. Recently, bacteriophages have been considered potential biological control agents for bacterial pathogens due to their host specificity. Pseudomonas syringae pv. actinidiae (Psa) is a reemerging pathogen that causes bacterial canker of kiwifruit (Actinidia sp.). The economic impact of this pest and the development of resistance to antibiotics and copper sprays in Psa and other pathovars have led to investigation of alternative management strategies. Phage therapy may be a useful alternative to conventional treatments for controlling Psa infections. Although the efficacy of bacteriophage φ6 was evaluated for the control of Psa, the characteristics of other DNA bacteriophages infecting Psa remain unclear. In this study, the PHB09 lytic bacteriophage specific to Psa was isolated from kiwifruit orchard soil. Extensive host range testing using Psa isolated from kiwifruit orchards and other Pseudomonas strains showed PHB09 has a narrow host range. It remained stable over a wide range of temperatures (4–50 °C) and pH values (pH 3–11) and maintained stability for 50 min under ultraviolet irradiation. Complete genome sequence analysis indicated PHB09 might belong to a new myovirus genus in Caudoviricetes. Its genome contains a total of 94,844 bp and 186 predicted genes associated with phage structure, packaging, host lysis, DNA manipulation, transcription, and additional functions. The isolation and identification of PHB09 enrich the research on Pseudomonas phages and provide a promising biocontrol agent against kiwifruit bacterial canker.

Amplification, Sequence and Expression of the Harpin Encoding Gene of the Bacterial Canker Pathogen, Pseudomonas syringae pv. syringae Strain NV

1999 ◽  
Vol 154 (4) ◽  
pp. 489-497
Author(s):  
Maryke Appel ◽  
Mathias Hampf ◽  
Lucienne Mansvelt ◽  
Janet Hapgood ◽  
Dirk Bellstedt
Keyword(s):  
Pseudomonas Syringae ◽  
Bacterial Canker ◽  
Canker Pathogen ◽  
Encoding Gene

Chemical control of bacterial canker (Pseudomonas syringae pv. syringae) of apricot and cherry in Victoria

1991 ◽  
Vol 31 (5) ◽  
pp. 705 ◽  
Author(s):  
DLS Wimalajeewa ◽  
R Cahill ◽  
G Hepworth ◽  
HG Schneider ◽  
JW Washbourne
Keyword(s):  
Pseudomonas Syringae ◽  
Chemical Control ◽  
Bordeaux Mixture ◽  
Weather Conditions ◽  
Hydrated Lime ◽  
Field Trials ◽  
Bacterial Canker ◽  
Copper Hydroxide ◽  
Low Concentrations ◽  
Excellent Control

Field trials were conducted during 1982-85, to develop a comprehensive spray program for the control of bacterial canker (Pseudomonas syringae pv, syringae) of apricot and cherry. Five spray schedules were evaluated as measures to reduce disease levels. Copper hydroxide at 2.5 g/L in water was applied to apricot, and bordeaux mixture at 6 g copper sulfate + 8 g hydrated lime/L in water was applied to cherry, during autumn, winter and pre-bloom spring. The effectiveness of copper sprays in reducing epiphytic populations of the pathogen during post-bloom spring was also tested. Copper hydroxide was applied to apricot, and a foliar copper nutrient and copper hydroxide were applied to cherry at low concentrations. Most spray schedules tested significantly (P<0.05) reduced canker incidence relative to controls. Excellent control of epiphytic populations of the pathogen on apricot and cherry was achieved with copper sprays applied at post-bloom in spring. A spray schedule consisting of 2 autumn, 1 winter and 2 pre-bloom spring sprays with copper hydroxide on apricot or bordeaux mixture on cherry was successful in reducing canker (>67% reduction) and is recommended for control of the disease. Two applications of copper hydroxide at 1.0 g/L in water in post-bloom spring considerably reduced (>9 1 %) epiphytic populations (P. syringae pv. syringae) on apricot and cherry leaves. Later sprays are recommended for use in combination with the autumn-winter-spring (pre-bloom) spray schedule, especially under excessively wet and cool weather conditions in spring.

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