scholarly journals Efecto antiinflamatorio de extractos etanólicos de Morinda citrifolia L. en conejos

Tequio ◽  
2019 ◽  
Vol 2 (6) ◽  
pp. 26-34
Author(s):  
Irma Cecilia Regino Álvarez ◽  
María de la Luz Ríos Vásquez ◽  
Héctor Ulises Bernardino Hernández ◽  
Arturo Zapién Martínez
Keyword(s):  
Secondary Metabolites ◽  
Leaf Extract ◽  
Adverse Reactions ◽  
Morinda Citrifolia ◽  
San Jose ◽  
Mature Leaves ◽  
Therapeutic Properties ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Inflammatory Effect

Morinda citrifolia L. has multiple therapeutic properties, particularly as an anti-inflammatory. The species has been widely studied, however, it is necessary to expand studies to document the presence of secondary metabolites and the anti-inflammatory effect of its extracts in animal models. The objective of the present paper was to identify the secondary metabolites present in the ethanol extracts of leaves and fruit of Morinda citrifolia L. collected in the community of San José Rio Manso, municipality of Lalana Choapam, Oaxaca, as well as the evaluation of its anti-inflammatory effect in rabbits. Ethanol extracts were obtained from the green and mature leaves, as well as the juice of the fruit. Green leaf extract gels were prepared and used in a completely randomized experiment in rabbits. The presence of alkaloids, leucoantacionins, amino acids, coumarins, cardiotonics, flavonoids, terpenes and tannins was identified. The 0.5% extract was more efficient at reducing the inflamed area by 55.6% compared to 28.6% of the control, 48 hours after the induction of inflammation. No signs of adverse reactions due to the use of the pharmaceutical forms evaluated were observed.

Effect of Topical Noni (Morinda citrifolia L.) Leaf Extract Paste in Carrageenan-induced Paw Edema on Wistar Rats

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Indah Puti Rahmayani Sabirin ◽  
Euis Reni Yuslianti
Keyword(s):  
Inflammatory Response ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Morinda Citrifolia ◽  
Paw Edema ◽  
Anti Inflammatory ◽  
Inflammatory Effect

The inflammatory response is one of natural process in the body to protect itself following tissue injury, but it can cause discomfort. Noni (Morinda citrifolia L.) leaf known as a traditional medicament to help reduce the inflammatory effect. The leaves empirically applied as a wrapping on fever or wound. The purpose of this study was to identify the anti-inflammatory effect of topical noni leaf extract paste in 5% and 10% concentration by examination of Wistar rat paw edema induced by λ-carrageenan. Twenty-four Wistar rats divided into four groups, which were negative control, positive control with diclofenac sodium 1% gel, 5% noni leaf, and 10% noni leaf paste groups. Paw edema was induced by intraplantar injection of 1% λ-carrageenan to every rat. Every treatment subsequently applied in the plantar area before injection, and the changed paw volume measured with plethysmometer at minutes 0, 30, 60, and 90. This study was at the Animal Laboratory, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi city in October–December 2017. The result displayed that the minimum volume after 90 minutes was on 5% and 10% noni leaf paste group, which is 1.00 mL. Kruskal-Wallis test result of inflammatory percentage was significantly different among every group in each examination time (p<0.05). Post-hoc test showed that inflammatory reduction on paw edema with noni leaf paste application on both concentrations were significantly different compared to the negative control. However, it was not different from the positive control group. This study showed that application of noni leaf paste in 5% and 10% concentration could help reduce inflammatory response on skin possibly by the active anti-inflammatory ingredients of noni leaf. EFEK PASTA EKSTRAK DAUN MENGKUDU (MORINDA CITRIFOLIA L.) TOPIKAL TERHADAP EDEMA KAKI TIKUS GALUR WISTAR YANG DIINDUKSI KARAGENANInflamasi adalah proses alami tubuh untuk melindunginya setelah cedera, namun hal tersebut dapat menyebabkan ketidaknyamanan. Daun mengkudu (Morinda citrifolia L.) dikenal sebagai obat tradisional untuk menurunkan efek inflamasi yang secara empiris digunakan untuk mengobati demam dan luka. Tujuan penelitian ini adalah mengetahui efek anti-inflamasi pasta ekstrak daun mengkudu melalui pemeriksaan edema kaki tikus yang diinduksi karagenan-λ. Dua puluh empat tikus galur Wistar dibagi menjadi 4 kelompok, yaitu kontrol negatif dengan akuades, kontrol positif (aplikasi gel Na diklofenak 1%), serta perlakuan pasta daun mengkudu 5% dan 10%. Edema dibuat dengan menginjeksi intraplantar tikus dengan 1% karagenan-λ pada tiap kelompok. Tiap-tiap perlakuan diaplikasikan sebelum tikus diinjeksi dan perubahan volume kaki tikus diukur dengan pletismometer di menit ke-0, 30, 60, dan 90. Penelitian ini dilakukan di Laboratorium Hewan, Fakultas Kedokteran, Universitas Jenderal Achmad Yani, Kota Cimahi pada Oktober–Desember 2017. Hasil pengukuran memperlihatkan penurunan volume edema kaki terkecil setelah 90 menit pada kelompok pasta daun mengkudu 10%, yaitu 1,00 mL. Hasil Uji Kruskal-Wallis terhadap persentase inflamasi berbeda nyata pada tiap kelompok dan tiap waktu pengamatan (p<0,05). Hasil uji beda menunjukkan bahwa penurunan inflamasi kaki tikus pada perlakuan pasta daun mengkudu kedua konsentrasi berbeda nyata dibanding dengan kontrol negatif, tetapi tidak berbeda dengan kontrol positif. Penelitian ini menunjukkan bahwa pasta daun mengkudu 5% dan 10% dapat membantu menurunkan reaksi inflamasi kulit dan efeknya sejalan dengan Na diklofenak karena zat aktif yang bersifat anti-inflamasi dalam daun mengkudu.

The Anti-oxidant Activity and Anti-inflammatory Effect of Rubus buergeri Miguel Leaf Extract

KSBB Journal ◽  
2019 ◽  
Vol 34 (4) ◽  
pp. 331-337
Author(s):  
Min-Seon Kim ◽  
Kyongwol Yang ◽  
Seung-Young Kim
Keyword(s):  
Leaf Extract ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi) sebagai Antioksidan dan Antiinflamasi

2019 ◽  
Vol 8 (3) ◽  
pp. 86
Author(s):  
Hasim Hasim ◽  
Yupi Yulianita Arifin ◽  
Dimas Andrianto ◽  
Didah Nur Faridah
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Phenolic And Flavonoid

Belimbing wuluh merupakan tanaman jenis buah dan obat tradisional. Tanaman belimbing wuluh sudah sering dimanfaatkan masyarakat salah satunya untuk mengobati penyakit seperti batuk dan radang rektum. Tujuan penelitian ini adalah menguji senyawa fitokimia, menganalisis kandungan total fenolik dan flavonoid, serta aktivitas antioksidan dan antiinflamasi secara in vitro pada ekstrak etanol daun belimbing wuluh. Metode yang dilakukan pada penelitian ini adalah ekstraksi daun belimbing wuluh, skrining fitokimia, perhitungan total fenol dan flavonoid, uji aktivitas antioksidan, dan uji aktivitas antiinflamasi. Senyawa fitokimia yang terkandung pada ekstrak etanol daun belimbing wuluh adalah saponin, tanin, steroid, flavonoid, dan alkaloid. Kandungan total fenolik dan flavonoid ekstrak etanol daun belimbing wuluh secara berturut-turut sebesar 39,03 dan 97,28 µg QE/mg. Ekstrak etanol daun belimbing wuluh memiliki aktivitas antioksidan yang tergolong sangat kuat, sementara aktivitas antiinflamasinya terutama ditunjukkan pada konsentrasi ekstrak 200 µg/ ml, memiliki nilai persen inhibisi hemolisis yang paling tinggi. Kesimpulannya, ekstrak etanol daun belimbing wuluh dapat menjadi salah satu sumber antioksidan dan antiinflamasi alami.Ethanol Extracts of Averrhoa Bilimbi Leaf Demonstrated Antioxidative and Anti-inflammatory ActivityAbstractAverrhoa bilimbi, a fruit-bearing plant, has been traditionally used for medicinal purposes such as treatment of cough and rectal inflammation. In this current work, ethanol extract of Averrhoa bilimbi leaf was analyzed, with regard to phytochemical composition, i.e. total phenolic, and flavonoid, antioxidant activity, and in vitro antiinflammation activity. Extraction of carambola leaves, phytochemical screening, total phenolic and flavonoid contens, assay of antioxidant activity, and assay of anti-inflammatory activity were done in this research. As a result, the leaf extract positively contained some phytochemical compounds, i.e. saponin, tannin, steroid, flavonoid, and alkaloid. Furthermore, total phenolic and flavonoid of the leaf extract was found at 39.03 and 97.28 µg QE/mg extract, respectively. Additionally, antioxidant activity of the leaf extract was classified as very strong, while its anti-inflammatory feature at extract concentration of 200 µg/ml exhibited the highest inhibition of hemolysis. In summary, the ability of Averrhoa bilimbi leaf ethanol extracts to act as antioxidative and anti-inflammatory agents was determined and this may open the use for natural antioxidant and anti-inflammatory agents.

scholarly journals PHYTOCHEMICAL SCREENING AND ANTI-INFLAMMATORY EFFECT TEST OF ETHANOL EXTRACT OF TURMERIC RHIZOME (Curcuma domestica Val.)TOWARD MALE WHITE MOUSE (Rattus norvegicus)

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Rulia Meilina
Keyword(s):  
Ethanol Extract ◽  
White Mouse ◽  
Phytochemical Screening ◽  
Suspension Control ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Group B ◽  
Group D ◽  
Better Than ◽  
Inflammatory Effect

Anti-inflammatory effect of ethanol rhizome of turmeric was conducted toward white mouse which is induced the carrageenan solution (lambda carrageenan) 1 %, research was conducted by dividing the animals into 5 groups.Group A was given CMC 0,5%, group B was given Indometacin with dose 100 mg/kgBB, group C was given  ethanol rhizome extract of turmeric with dose 400 mg/kgBB, group D was given ethanol rhizome extract of turmeric with dose 500 mg/kgBB, group E was given ethanol rhizome extract of turmeric with dose 600 mg/kgBB. Result of phytochemical screening toward powder and ethanol extract of rhizome of turmeric shows that chemical compounds which are involved are alkaloids, flavonoids, and aetheric oil. In the other hand, result of phytochemical screening toward ethanol extract of turmeric’s rhizome are alkaloids,flavonoids,anthraquinones, glycosides. Result of anti-inflammatory test shows that there is the real difference among groups of mouse which were given CMC suspension (control), suspension of ethanol extracts of turmeric’s rhizome with dose 400 mg/kg BB, 500 mg/kg BB, 600 mg/kg BB (material test) and suspension of indomethacin with dose 100 mg/kg BB (positive comparison). Suspension of ethanol extract of turmeric’s rhizome with dose 600 mg/kg BB shows that anti-inflammatory capability is better than dose 400 mg/kg BB and 500 mg/kg BB, and suspension of ethanol extract of turmeric’s rhizome dose 600 mg/kg BB shows the anti-inflammatory effect which is not really different with suspension of indomethacin with dose 100 mg/kg BB, it is showed by  ANOVA statistic analysis among those comparisons toward indomethacin suspension as positive comparison.Keywords:Anti-inflammatory, Indomethacin, Turmeric Rhizome

Chemical structure and anti-inflammatory effect of polysaccharide extracted from Morinda citrifolia Linn (Noni)

2018 ◽  
Vol 197 ◽  
pp. 515-523 ◽  
Author(s):  
Stefany Guimarães Sousa ◽  
Lorena Almeida Oliveira ◽  
Diva de Aguiar Magalhães ◽  
Tarcisio Vieira de Brito ◽  
Jalles Arruda Batista ◽  
...  
Keyword(s):  
Chemical Structure ◽  
Morinda Citrifolia ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Antinociceptive and anti-inflammatory properties of Tetracera alnifolia Willd. (Dilleniaceae) hydroethanolic leaf extract

2018 ◽  
Vol 30 (2) ◽  
pp. 173-184 ◽  
Author(s):  
Olufunmilayo O. Adeyemi ◽  
Ismail O. Ishola ◽  
Elizabeth T. Adesanya ◽  
Destiny O. Alohan
Keyword(s):  
Nitric Oxide ◽  
Acetic Acid ◽  
Potassium Channel ◽  
Leaf Extract ◽  
Paw Edema ◽  
Hot Plate ◽  
Cotton Pellet ◽  
Anti Inflammatory ◽  
Tetracera Alnifolia ◽  
Inflammatory Effect

Abstract Background Tetracera alnifolia Willd. (Dilleniaceae) is used in traditional African Medicine for the treatment of headache, abdominal pain, and rheumatism. Hence, this study sought to investigate the antinociceptive and anti-inflammatory effects of the hydroethanolic leaf extract of T. alnifolia (HeTA) in rodents. Methods Antinociceptive activity was evaluated using the acetic acid-induced writhing, formalin-/capsaicin-induced paw licking and hot plate tests in mice. The contribution of opioidergic, l-arginine-nitric oxide, and ATP-sensitive potassium channel pathways in HeTA-induced antinociception was also evaluated. The anti-inflammatory effect was assessed using the carrageenan-induced paw edema, xylene ear edema, cotton pellet granuloma, and complete Freund’s adjuvant (CFA)-induced arthritis in rats. Results HeTA (100, 200, and 400 mg/kg, p.o.) produced significant (p<0.05) decrease in mean number of acetic acid-induced writhing, time spent licking paw in formalin, and capsaicin tests as well as time course increase in nociceptive reaction latency in hot plate test. HeTA-induced antinociception was prevented by pretreatment of mice with naloxone (non-selective opioid receptor antagonist), l-arginine (nitric oxide precursor), or glibenclamide (ATP-sensitive potassium channel blocker). HeTA (100 mg/kg, p.o.) produced a significant anti-inflammatory effect against carrageenan-induced rat paw edema (1–5 h), xylene-induced ear edema, cotton pellet-induced granuloma formation, and CFA-induced arthritis in rats. The effects of HeTA in various models were similar to the effect of the standard reference drugs. Conclusions Findings from this study showed that HeTA possesses antinociceptive effect possibly mediated through peripheral opioid receptors with activation of l-arginine-nitric oxide and ATP-sensitive potassium channel pathway as well as anti-inflammatory activity.

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