3 FACTORIAL DESIGN FOR OPTIMISATION OF COLORIMETRIC METHOD FOR QUANTIFICATION OF CAPTOPRIL

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (09) ◽  
pp. 47-52
Author(s):  
S. S Desai ◽  
◽  
K. A Dave ◽  
J. D. Naik ◽  
A. B. Yadav
Keyword(s):  
Factorial Design ◽  
Ferric Chloride ◽  
Regression Coefficient ◽  
Colorimetric Method ◽  
Chloride Concentration ◽  
Potassium Ferricyanide ◽  
Optimum Concentration ◽  
Design Expert ◽  
Method Effect ◽  
Ich Guidelines

Captopril was quantitatively determined by colorimetry using ferric chloride and potassium ferricyanide. 33 factorial design with help of design expert software (version 9.0.4) was used for optimisation of method. Effect of method variables such as concentration of ferric chloride, concentration of potassium ferricyanide and volume of both reagents was evaluated on method response absorbance. Optimum concentration of ferric chloride and potassium ferricyanide was found to be 0.3% and 0.2%, respectively and optimum volume of both reagents was found to be 1.5 mL. The developed method was validated as per ICH guidelines. The linearity of the proposed method was found in the concentration range of 1.0 – 6.0 μg/mL with regression coefficient (R2) of 0.9913. The % recovery was found between 98.23- 104.25 %. The method was found to be precise as the values of % RSD obtained were found to be

scholarly journals SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION AMIKACIN IN PURE AND PHARMACEUTICAL DOSAGE FORM

2018 ◽  
Vol 10 (1) ◽  
pp. 38
Author(s):  
Surya Teja G. ◽  
Gurupadayya B. M. ◽  
Venkata Sairam K.
Keyword(s):  
Dosage Form ◽  
Regression Coefficient ◽  
Spectroscopic Method ◽  
Chloranilic Acid ◽  
Pharmaceutical Dosage Form ◽  
Ich Guidelines ◽  
Highly Sensitive ◽  
Acid Reagent ◽  
Chromophoric Group ◽  
Charge Transfer Complexation

Objective: The aim of the study was to develop an easy, sensible and rapid method for the estimation of amikacin in both pure and marketed formulation using the spectrophotometric method.Methods: Due to lack of chromophoric group in the amikacin, it was derivatized with 0.1 mmol chloranillic acid reagent. For the estimation of amikacin, Shimadzu UV-1700 model spectrophotometer with UV probe software was used. The method was based simple on charge transfer complexation of the drug with a p-chloranilic acid reagent to give a purple coloured product which was measured at 524 nm against blank solution.Results: The derivatised product of amikacin was detected at a wavelength of 524 nm. Linearity was observed with the concentration range of 20-100 µg/ml with a regression coefficient of 0.9803. Results of all the parameters were within the acceptance criteria with % RSD less than 2.Conclusion: The spectroscopic method was validated as per ICH guidelines and was found to be applicable for routine quantitative analysis of amikacin in marketed formulations also. The results of linearity, precision, accuracy LOD and LOQ were within the specified limits. The method is highly sensitive, robust, reproducible and specific.

scholarly journals Spectrophometric Determination of Nelfinavir Mesylate

2006 ◽  
Vol 3 (2) ◽  
pp. 78-82 ◽  
Author(s):  
K. Vanitha Prakash ◽  
Jangala Venkateswara Rao
Keyword(s):  
Ferric Chloride ◽  
Dosage Form ◽  
Potassium Ferricyanide ◽  
Reagent Blank ◽  
Spectrophotometric Methods ◽  
Bluish Green ◽  
Nelfinavir Mesylate ◽  
Bulk Drug ◽  
Tablet Dosage

Two new simple, sensitive, rapid and economical Spectrophotometric Methods (A and B) have been developed for the determination of Nelfinavir Mesylate in pharmaceutical bulk and tablet dosage form. The method A is based on the reaction of Nelfinavir with ferric chloride, potassium ferricyanide and hydrochloric acid to form a bluish green colored chromogen. The Method B is based on the formation of blood red colored chromogen with Ferric chloride and 1,10-phenanthroline. The absorbances of the chromogen were measured at their respective wavelength of maximum absorbance against the corresponding reagent blank. The proposed methods have been successfully applied to the analysis of the bulk drug and its tablet dosage form. The methods have been statistically evaluated and were found to be precise and accurate.

A COLORIMETRIC METHOD FOR THE ESTIMATION OF REDUCING GROUPS IN MILK POWDERS

1945 ◽  
Vol 23b (3) ◽  
pp. 91-99 ◽  
Author(s):  
R. A. Chapman ◽  
W. D. McFarlane
Keyword(s):  
Amino Acids ◽  
Positive Reaction ◽  
Colorimetric Method ◽  
Protein Molecule ◽  
Potassium Ferricyanide ◽  
Potassium Iodate ◽  
Detection And Estimation ◽  
Reducing Activity ◽  
Colorimetric Estimation

A method has been developed for the detection and estimation of certain reducing groups in milk powders. The method is based on the reduction of potassium ferricyanide at 70 °C. and pH 5 and colorimetric estimation of ferric ferrocyanide. Titration with 2,6-dichlorophenolindophenol or potassium iodate failed to show any difference between fresh and stale milk powders. In contrast the ferricyanide test indicates much higher values in fresh powders than do the titration methods, and it also reveals a marked increase in samples that have developed stale, musty odours and flavours. Lactalbumin and casein show reducing activity that increases on heating. Of the amino acids tested, only tryptophane gives a positive reaction. It is concluded that the reducing groups are present in the protein molecule and become accessible on denaturation.

scholarly journals A novel sensitive colorimetric determination of catecholamine drug in dosage form via oxidative coupling reaction with MBTH and potassium ferricyanide

2021 ◽  
Vol 5 (1) ◽  
pp. 51-58
Author(s):  
Safwan Ashour
Keyword(s):  
Oxidative Coupling ◽  
Colorimetric Method ◽  
Standard Free Energy ◽  
Coupling Reaction ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Potassium Ferricyanide ◽  
Colorimetric Determination ◽  
Oxidative Coupling Reaction

A new and direct colorimetric method has been established for the determination of catecholamine (methyldopa, MD) in both pure form and in pharmaceutical formulations. The method is based on the oxidative coupling reaction of MD with 3-methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate (MBTH) and potassium ferricyanide at pH 10.4 in aqueous medium to form an orange product that has a maximum absorption at 460 nm. Beer's law plot showed good correlation in the concentration range of 1.0−56.0 µg mL-1, with detection limit of 0.31 µg mL-1. Molar absorptivity for the above method was found to be 6.56×103 L mol-1 cm-1. All the measurements were carried out at 25 ± 1.0 °C, the formation constant (logKf) value of colored species is 9.48 and the standard free energy (DG‡) is − 54.09 KJ mol-1. This method was applied successfully to determination of MD in tablets and the results were compared with the USP method. Common excipients used as additives in tablets do not interfere in the proposed method. The method is accurate, precise and highly reproducible, while being simple, cheap and less time consuming and hence can be suitably applied for routine analysis of MD in bulk and dosage forms.

scholarly journals Datura stramonium Leaves Mucilage Aided Buccoadhesive Films of Aceclofenac using 32 Factorial Design with Design-Expert Software

2021 ◽  
Vol 55 (2s) ◽  
pp. s396-s404
Author(s):  
Hindustan Abdul Ahad ◽  
Haranath Chinthaginjala ◽  
Manchikanti Sai Priyanka ◽  
Dasari Rahul Raghav ◽  
Madana Gowthami ◽  
...  
Keyword(s):  
Factorial Design ◽  
Datura Stramonium ◽  
Design Expert ◽  
32 Factorial Design

scholarly journals Evaluating the influence of Citrullus lanatus seed extracts on electrolytes, urea and creatinine in Streptozotocin induced diabetic albino rats

2018 ◽  
pp. 87-94
Keyword(s):  
Sodium Chloride ◽  
Citrullus Lanatus ◽  
Colorimetric Method ◽  
Chloride Concentration ◽  
Ethanolic Extract ◽  
Sodium Concentration ◽  
Diabetic Control ◽  
Albino Rats ◽  
Potassium Sodium ◽  
Group 1

The focus of current research study was to evaluate the influence of the seed extract of Citrullus lanatus on urea, creatinine, potassium, sodium, chloride and bicarbonate in streptozotocin induced diabetic rats. Eighteen male wistar albino rats were divided into six groups of three rats each, diabetes were induced in all the rats except group 1 by intraperitoneal injection of 45 mg/kg b.wt. of streptozotocin. Group 1 rats served as control and received standard feed and water daily; Group 2 rats received oral Glibenclamide (0.5 mg/kg bw); Group 3,4, and 5 received 200 mg/kg bw, 400 mg/kg bw and 600 mg/kg b.wt. of the ethanolic extract of Citrullus lanatus seed respectively; and group 6 served as diabetic group. Blood samples were collected and analysed for urea, creatinine, potassium, sodium, chloride and bicarbonate using urease-Berthelot colorimetric method, Jaffe’s colorimetric method and Ion selective electrode (ISE) method respectively. There was a significant decrease in (p<0.05) glucose concentration (mmol/L) of 3.90 in control compared to 8.07, 8.73, 14.67, 11.43 and 9.80 in albino rats treated with glibenclamide, STZ+CLS 200 mg, STZ+CLS 400 mg, STZ+CLS 600 mg and diabetic control respectively. In potassium concentration (meq/l) 6.51 in control compared with 4.97, 7.59, 7.28, 8.45 and 6.87 in albino rats treated with glibenclamide, STZ+CLS200 mg, STZ+CLS 400 mg, STZ+CLS 600 mg and diabetic control respectively. While in sodium concentration (meq/l) of 1.42 in control compared to 1.33, 1.30, 1.36, 1.42 and 1.36 in albino rats treated with glibenclamide, STZ+CLS 200 mg, STZ+CLS 400 mg, STZ+CLS 600 mg and diabetic control respectively. However, in chloride concentration (meq/l) of 1.06 in control compared to 99.10, 97.87, 1.04, 88.00, 1.04, 88.00 and 1.04 in albino rats treated with glibenclamide, STZ+CLS 200 mg, STZ+CLS 400 mg, STZ+CLS 600 mg and diabetic control respectively and also in bicarbonate concentration (meq/l) of 10.07 when compared with 17.30, 16.47, 15.40, 6.40 and 17.30 in albino rats treated with glibenclamide, STZ+CLS 200 mg, STZ+CLS 400 mg, STZ+CLS 600 mg and diabetic control respectively. In conclusion, this medicinal plant could be considered as a potential and alternative approach for the treatment of diabetes. Keywords: Keywords: Citrullus lanatus, Diabetes Mellitus, Renal

scholarly journals Ferric reducing anti-oxidant power assay in plant extract

2016 ◽  
Vol 11 (3) ◽  
pp. 570 ◽  
Author(s):  
Maruthamuthu Vijayalakshmi ◽  
Kandasamy Ruckmani
Keyword(s):  
Ascorbic Acid ◽  
Ferric Chloride ◽  
Trichloroacetic Acid ◽  
Methanolic Extract ◽  
Video Clip ◽  
Potassium Ferricyanide ◽  
Buffer Solution ◽  
Solution Ph ◽  
Frap Assay ◽  
Anti Oxidant

<p>The ferric reducing anti-oxidant power (FRAP) assay involved the following steps:  a) preparation of samples, b) reactions and c) finally measuring absorbance of sample and standard at 700 nm using spectrophotometer. The samples were methanolic extract, different fractions (<em>n</em>-hexane, chloroform) and standard ascorbic acid. The solutions prepared were buffer solution (pH 6.6), 1% potassium ferricyanide solution, 10% trichloroacetic acid and 0.1% ferric chloride. </p><p><strong>Video Clip</strong></p><a href="https://www.youtube.com/v/s7BpxNbN5dE">Ferric reducing anti-oxidant power assay:</a>21 min 18 sec<p> </p>

8. On Ferric Ferricyanide as a Reagent for Detecting Traces of Reducing Gases

1888 ◽  
Vol 14 ◽  
pp. 419-419
Author(s):  
Crum Brown
Keyword(s):  
Prussian Blue ◽  
Ferric Chloride ◽  
Filter Paper ◽  
Reducing Agents ◽  
Potassium Ferricyanide ◽  
Reducing Gases ◽  
Sulphuretted Hydrogen

The brown solution obtained when solutions of ferric chloride and potassium ferricyanide are mixed, which may be regarded as containing ferric ferricyanide, is, as is well known, very readily turned blue by reducing agents, Prussian blue or Turnbull's blue being formed. The author uses strips of filter paper dipped in the freshly prepared solution to test for traces of reducing gases, such as sulphuretted hydrogen, sulphurous acid, &c. As nitrous fumes also blue the brown solution, reducing it, traces of them can be detected by using together a piece of paper prepared as above and a piece of iodised starch paper.

Rapid Colorimetric Method for the Determination of Methyl Salicylate in Medicated Oils

1966 ◽  
Vol 49 (4) ◽  
pp. 854-856
Author(s):  
M C Dutt
Keyword(s):  
Aqueous Solution ◽  
Ferric Chloride ◽  
Calibration Curve ◽  
Methyl Salicylate ◽  
Quantitative Estimation ◽  
Colorimetric Method ◽  
Synthetic Mixture ◽  
Excess Water ◽  
Ether Solution

Abstract The well-known reaction between methyl salicylate and ferric chloride has been successfully applied to the quantitative estimation of methyl salicylate in medicated oils. An aqueous solution of ferric chloride is added to an ether solution of medicated oil dissolved in excess water. After filtering, the absorbance of the filtrate is measured at 530 m/j, and the content of methyl salicylate is determined from a calibration curve. Methyl salicylate recoveries range from 92 to 97% for concentrations between 10 and 60% in a synthetic mixture. The method is simple and rapid, and compares favorably with Wilson’s method.

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