scholarly journals Recovery of equine oocytes by scraping of the follicular wall with different specifications of needles and morphological analysis of cumulus oophorus

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4333
Author(s):  
Suellen Miguez González ◽  
Camila Bizarro da Silva ◽  
Andressa Guidugli Lindquist ◽  
Isabela Búfalo ◽  
Fernanda Zandonadi Machado ◽  
...  
Keyword(s):  
Tunica Albuginea ◽  
Cumulus Cells ◽  
Recovery Rates ◽  
Follicular Aspiration ◽  
Cumulus Oophorus ◽  
Grade Ii ◽  
Student’S T ◽  
Group B ◽  
Grade Iii

In follicular aspiration, physical aspects are of high significance for the technique to succeed, such as vacuum pressure, caliber of the needle and the way the follicular wall curettage is performed. The aim of this study was to investigate the recovery rate of equine oocytes aspirated by scraping of the follicular wall, testing different calibers of disposable needles, as well as the morphological evaluation of the cumulus oophorus complexes (COCs). Mares ovaries (n=447) obtained at a local slaughterhouse were transported to the laboratory in a thermal container (20 °C) and had the tunica albuginea and connective tissues dissected. The aspirated follicles had 10 to 25 mm in diameter, and 30x8 (21G 1 ¼) or 40x12 (18G 1 ½) needles were used for the aspiration, forming group A (G-A) and group B (G-B), respectively. In G-A and G-B, 480 and 548 follicles were aspirated, respectively. Under the stereomicroscope, the oocytes were evaluated according to the quality of the ooplasm and characteristics of the cumulus cells (grade I, II, III and denuded). The statistical analysis was performed using the Student’s t-test, logistic regression and test of proportions, and differences were considered significant when P<0.05. There was no difference between recovery rates of groups G-A (66.5%; 330/496) and G-B (65.5%; 359/548). In the G-A group, grade II oocytes were related to higher recovery rates (46.9%; 145/330) than grade I (23.6%; 72/330), grade III (20.6%; 59/330) and denuded oocytes (8.5%; 24/330; P<0.05). However, in G-B, there was no statistical difference regarding the quality of the recovered oocytes: grade I (23.4%; 77/359), grade II (43.2%; 145/359), grade III (22.5%; 73/359) and denuded (11.1%; 32/359). The 30x8 (21G 1 ¼) needle provided a higher proportion of grades I and II oocytes than the 40x12 (18G 1 ½) needle, with 72.4% (239/330) and 65% (233/359; P<0.05), respectively. Both calibers of needles tested in this study provide efficient oocyte recovery rates. Aspiration with 30x8 (21G 1 ¼) needles resulted in a higher proportion of morphologically good equine oocytes for use in reproductive biotechnologies.

scholarly journals Recovery of equine oocytes by scraping of the follicular wall with different specifications of needles and morphological analysis of cumulus oophorus

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4333 ◽  
Author(s):  
Suellen Miguez González ◽  
Camila Bizarro da Silva ◽  
Andressa Guidugli Lindquist ◽  
Isabela Búfalo ◽  
Fernanda Zandonadi Machado ◽  
...  
Keyword(s):  
Tunica Albuginea ◽  
Cumulus Cells ◽  
Recovery Rates ◽  
Follicular Aspiration ◽  
Cumulus Oophorus ◽  
Grade Ii ◽  
Student’S T ◽  
Group B ◽  
Grade Iii

<p>In follicular aspiration, physical aspects are of high significance for the technique to succeed, such as vacuum pressure, caliber of the needle and the way the follicular wall curettage is performed. The aim of this study was to investigate the recovery rate of equine oocytes aspirated by scraping of the follicular wall, testing different calibers of disposable needles, as well as the morphological evaluation of the cumulus oophorus complexes (COCs). Mares ovaries (n=447) obtained at a local slaughterhouse were transported to the laboratory in a thermal container (20 °C) and had the tunica albuginea and connective tissues dissected. The aspirated follicles had 10 to 25 mm in diameter, and 30x8 (21G 1 ¼) or 40x12 (18G 1 ½) needles were used for the aspiration, forming group A (G-A) and group B (G-B), respectively. In G-A and G-B, 480 and 548 follicles were aspirated, respectively. Under the stereomicroscope, the oocytes were evaluated according to the quality of the ooplasm and characteristics of the cumulus cells (grade I, II, III and denuded). The statistical analysis was performed using the Student’s t-test, logistic regression and test of proportions, and differences were considered significant when P&lt;0.05. There was no difference between recovery rates of groups G-A (66.5%; 330/496) and G-B (65.5%; 359/548). In the G-A group, grade II oocytes were related to higher recovery rates (46.9%; 145/330) than grade I (23.6%; 72/330), grade III (20.6%; 59/330) and denuded oocytes (8.5%; 24/330; P&lt;0.05). However, in G-B, there was no statistical difference regarding the quality of the recovered oocytes: grade I (23.4%; 77/359), grade II (43.2%; 145/359), grade III (22.5%; 73/359) and denuded (11.1%; 32/359). The 30x8 (21G 1 ¼) needle provided a higher proportion of grades I and II oocytes than the 40x12 (18G 1 ½) needle, with 72.4% (239/330) and 65% (233/359; P&lt;0.05), respectively. Both calibers of needles tested in this study provide efficient oocyte recovery rates. Aspiration with 30x8 (21G 1 ¼) needles resulted in a higher proportion of morphologically good equine oocytes for use in reproductive biotechnologies.</p><p> </p>

scholarly journals Elastic intramedullary nailing combined with methylprednisolone acetate injection for treatment of unicameral bone cysts in children: a retrospective study

2020 ◽  
pp. 1-8
Author(s):  
JianWu Zhou ◽  
ShangKun Ning ◽  
Yuxi Su ◽  
Chuankang Liu
Keyword(s):  
Blood Loss ◽  
Intramedullary Nailing ◽  
Methylprednisolone Acetate ◽  
Bone Cysts ◽  
Hospitalization Time ◽  
Group A ◽  
Grade Ii ◽  
Group B ◽  
Elastic Intramedullary Nailing ◽  
Grade Iii

Purpose The aetiology of unicameral bone cysts (UBCs) is unclear. This study aims to evaluate the feasibility of elastic intramedullary nailing (EIN) combined with injections of methylprednisolone acetate (MPA) for the treatment of UBCs in children. Methods We retrospectively analyzed 53 children with UBCs in our hospital between January 2010 and April 2016. A total of 24 patients (Group A) were treated by EIN and MPA, whilst 29 patients (Group B) were treated by curettage, bone grafts and EIN fixation. The radiographs of the UBCs were evaluated following the Capanna criteria. All patients were followed-up on the third, sixth, 12th, 24th and 36th months. Fixation time, hospitalization time and complications were evaluated. Results In Group A, the mean number of MPA injections was 1.8 (1 to 3). Based on radiographic evaluation, eight patients were healed (Capanna grade I), 14 were healed with residual cysts (Capanna grade II), one showed recurrence (Capanna grade III) and one showed no response to the treatment (Capanna grade IV). In Group B, 11 patients were evaluated as Capanna grade I, 12 as Capanna grade II, three as Capanna grade III and three as Capanna grade IV. There was significant difference in the early postoperative function activity (p < 0.001), hospitalization time (p = 0.028), blood loss during surgery (p < 0.001) and surgery time (p < 0.001). Conclusion The combination of EIN and MPA for the treatment of UBCs in children is feasible, has little operative trauma, short surgery time, short hospitalization time, less blood loss and a low risk of incision infection. Level of evidence: III

Grading Fish for Quality. 2. Statistical Analysis of the Results of Experiments Regarding Grades and Trimethylamine values

1958 ◽  
Vol 15 (4) ◽  
pp. 717-728 ◽  
Author(s):  
P. L. Hoogland
Keyword(s):  
Statistical Analysis ◽  
Linear Regression ◽  
Recent Work ◽  
Statistical Treatment ◽  
Regression Equation ◽  
Linear Regression Equation ◽  
Grade Ii ◽  
Set Up ◽  
Grade Iii

The results of recent work at this station regarding the organoleptic grade of cod and haddock and the trimethylamine content of these fish were subjected to statistical treatment. It was established that there is a relation between grade and trimethylamine content which may be represented by a linear regression equation, if the trimethylamine content is expressed as log (1 + TMA value). The confidence with which this TMA index may be used to estimate grade was also determined. Based on these findings, a scale of TMA values for use in practical grading was set up; the suggested values are grade I: TMA value 0.00 to 1.00; grade II: TMA value 1.01 to 7.00 and grade III: TMA value 7.01 and higher. Finally, a method is suggested by means of which the ability of a grader to judge the quality of fish may be assessed.

135 Effect of Different Bovine Breeds on Efficiency of Embryo Production by Ovum Pick-Up-IVF

2018 ◽  
Vol 30 (1) ◽  
pp. 207
Author(s):  
S. Sato ◽  
M. Moriyoshi ◽  
O. Dochi ◽  
K. Imai
Keyword(s):  
Calf Serum ◽  
Cumulus Cells ◽  
Holstein Cows ◽  
Blastocyst Formation ◽  
Culture Dish ◽  
Embryo Production ◽  
Chi Squared ◽  
Student’S T ◽  
Kinetics Of

Production of bovine embryos by ovum pick-up (OPU)-IVF has increased in recent years. However, numerous factors affect efficiencies of embryo production using this technology. This study was investigated the effects of different breeds on embryo production by OPU-IVF. In total, 98 OPU-IVF sessions were conducted on 41 Holstein and 27 Japanese Black cows from February 2015 to May 2017. The collected cumulus–oocyte complexes (COC) were matured for 22 h. After co-culture of COC with sperm for 6 h, the presumptive zygotes were washed and denuded by pipetting. Those zygotes were then cultured in CR1aa medium supplemented with 5% calf serum for 9 days in a micro-well culture dish. Blastocyst formation rates were analysed at 9 days post-insemination (hpi). The kinetics of embryo development was observed at 27, 31, and 55 hpi. Four factors were considered for selecting embryos to predict pregnancy competence: (1) timing of first cleavage; (2) formation of 2 blastomeres after first cleavage at 31 hpi; (3) absence of fragments after first cleavage at 31 hpi; (4) formation of 8 or more blastomeres at 55 hpi. The quality of blastocysts was compared with the proportions of embryos that were selected based on these 4 factors. Data were analysed by the Chi-squared test with Yates’ correction and Student’s t-test. Total numbers of COC from Holstein or Japanese Black cows were 1330 (n = 51) and 1543 (n = 47), respectively. However, no differences were observed in the numbers of collected and cultured COC per OPU session between Holstein (26.1 ± 20.1 and 21.7 ± 20.1) and Japanese Black (32.8 ± 24.7 and 28.7 ± 22.7) cows. The percentage of COC linked into Grade 1, 2, or 3 was 47.6, 32.1, and 10.7% for Japanese Black and 45.8, 25.6, and 12.2% for Holstein cows, respectively. The proportion of COC with expanded cumulus cells was significantly higher (P < 0.01) in Holstein (12.7%) than in Japanese Black (7.7%) cows. The number of blastocyst and transferable embryo production per OPU session in Holstein (7.5 ± 8.3 and 7.3 ± 7.7) cows was not different from that in Japanese Black (10.6 ± 9.3 and 9.8 ± 9.0) cows. Moreover, there was no difference in blastocyst formation rates between Japanese Black (36.8%) and Holstein (34.8%) cows. Percentages of embryos selected by factors 1 to 4 were 42.6, 66.8, 59.1, and 54.4% in Holstein, and 54.6, 83.2, 72.2, and 75.2% in Japanese Black cows, respectively. Proportions of blastocysts in Holstein and Japanese Black cows selected by a combination of all 4 factors were 21.6 and 36.1%, respectively. There were significant differences (P < 0.01) between the 2 breeds in terms of proportion of blastocysts selected based on individual factors as well as a combination of all 4 factors. These results suggested that the efficiency of embryo production did not differ between Japanese Black and Holstein cows. However, the quality of embryos selected by a combination of 4 factors was significantly higher in Japanese Black than in Holstein cows.

scholarly journals 10-Year Journey Through Brain Gliomas: Case Report

2020 ◽  
Vol 25 (3) ◽  
pp. 26-28
Author(s):  
Cosmin-Nicodim Cîndea ◽  
Adriana Săceleanu
Keyword(s):  
Statistical Data ◽  
Medical Profession ◽  
Doctor Who ◽  
Wild Type ◽  
Cerebral Gliomas ◽  
Different Types ◽  
Grade Ii ◽  
Brain Gliomas ◽  
Grade Iii

AbstractWe present the case of a 28-year-old doctor who was diagnosed in 2009 with grade II oligodendroglioma. In the next 10 years he underwent 4 surgeries, passing in turn through 3 different types of cerebral gliomas: grade II oligodendroglioma, grade III oligoastrocytoma and IDH Wild-Type glioblastoma. In the spirit of the surgical resection limited by the preservation of the function, he was able to practice his medical profession until the last months of his life. Life expectancy at the time of diagnosis complied with all the statistical data. Although the etiology, pathophysiology and treatment of cerebral gliomas is still full of unknowns, knowing our limits we can provide a good quality of life for our patients.

Effectiveness of Bypass and Sleeve Bariatric Surgical Methods in Weight Reduction

2021 ◽  
Vol 7 (4) ◽  
pp. 1-5
Author(s):  
Cristianne Confessor Castilho Lopes ◽  
Keyword(s):  
Surgical Technique ◽  
Surgical Techniques ◽  
Psychosocial Problems ◽  
Morbid Condition ◽  
Endocrine Disease ◽  
Surgical Methods ◽  
Effective Option ◽  
Grade Ii ◽  
Grade Iii

Obesity is a chronic disease described by the large amount of body fat that leads to an increase in Body Mass Index (BMI). It is subdivided into three levels, being grade I with a BMI between 30 and 34.9 kg/m2, grade II between 35 and 39.9 kg/m2 and grade III or morbid obesity with a BMI above 40 kg/m2. Aiming at reversing the morbid condition caused by the disease, the bariatric surgical technique presents itself as the most effective option to contain grade III obesity, as well as the associated co morbidities. The objective of surgical techniques is to improve the quality of life of the obese, reducing both physical and psychosocial problems. As endocrine disease is related to several co morbidities, such as high blood pressure, sleep apnea and even some types of cancer, it is necessary to carry out an investigation into the surgical technique to be applied as well as its effectiveness during and after the surgical procedure.

250 EFFICIENCY COMPARISON BETWEEN FOLLICULAR ASPIRATION AND SCRAPING FOR EX VIVO RECOVERY OF EQUINE CUMULUS ENCLOSED OOCYTES

2015 ◽  
Vol 27 (1) ◽  
pp. 214
Author(s):  
J. Walter ◽  
S. Jaeger ◽  
U. Bleul
Keyword(s):  
Follicular Fluid ◽  
Ex Vivo ◽  
Cumulus Cells ◽  
Well Being ◽  
Developmental Competence ◽  
Recovery Rates ◽  
Cumulus Oophorus ◽  
Oocyte Recovery ◽  
Oocyte Aspiration ◽  
Efficiency Comparison

Collection of cumulus enclosed oocytes from excised ovaries has become an ambitious task in recent years as a result of a decrease in slaughtering of horses because of ethical and political reasons. This increases the demand for highly efficient procedures to collect the maximum amount of oocytes out of these ovaries. The main objective of this study was to compare the efficiency of 2 oocyte recovery procedures. Oocyte aspiration is a quick and easy-to-perform technique with one potential limitation: the loss of the cumulus oophorus through suction forces. Recovery by follicular scraping is a more laborious procedure, but seems to be superior with regard to collection of cumulus-enclosed oocytes. This study was conducted to figure out the most efficient technique to collect high-quality equine cumulus-enclosed oocytes. The study was performed with ovaries of 32 slaughtered mares on 9 experimental days. All follicles of both ovaries from each mare were randomly allocated either to oocyte recovery by aspiration (15 mares) or by scraping (17 mares). Follicular scraping was performed with a bone curette after incision of the follicle with a scalpel blade, and follicular fluid was evacuated in watch glasses. After scraping, the follicles were flushed with PBS. Aspiration was performed using an 18-gauge needle with Tuohy bevel on a syringe. In a first step, follicular fluid was aspirated using scraping movements with the needle on the follicular wall. The empty follicular cavity was flushed once with the follicular fluid, the aspiration fluid was transferred to Falcon tubes and the aspirate settled for 10 min. Sediment of aspirated follicles was transferred to culture dishes, oocytes were searched under the stereomicroscope, and scraped follicles were searched directly in the watch glasses. Oocytes were classified in oocytes with compact cumulus oophorus, oocytes with expanded cumulus oophorus, oocytes with corona radiata only, denuded oocytes, and degenerated oocytes. Recovery rates were analysed for the 2 methods with special regard to oocyte classification. The overall recovery rates were similar for scraped (53.74%) and aspirated (52.70%) follicles. The 2 methods performed differently with respect to oocyte qualities. Significantly more of the oocytes recovered by scraping were collected with compact cumulus oophorus (72.16%) than in the aspiration group (33.77%; P = 0.004). Both methods collected similar amounts of oocytes with expanded cumulus oophorus (10.12% by scraping, 7.79% by aspiration). Using the aspiration more oocytes presented denuded (24.67 v. 6.33%, P = 0.08). The results of the presented study confirm that the scraping procedure is superior with regard to collection of cumulus-enclosed oocytes. These results prioritize follicle scraping for ex vivo collection of oocytes for research purposes because cumulus cells may support oocytal well-being during maturation and represent a unique biomarker source for the developmental competence of their corresponding oocytes.

189 EFFECT OF THE OVARIAN STIMULATION OF ANESTROUS CATS WITH eCG ON MORPHOLOGICAL QUALITY AND GENE EXPRESSION PROFILE OF CUMULUS-OOCYTE COMPLEXES

2017 ◽  
Vol 29 (1) ◽  
pp. 203 ◽  
Author(s):  
D. Veraguas ◽  
S. R. Cuevas ◽  
P. F. Gallegos ◽  
F. O. Castro ◽  
L. Rodriguez-Alvarez
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Polar Body ◽  
Relative Expression ◽  
First Polar Body ◽  
Maturation Rate ◽  
Grade Ii ◽  
Grade Iii

The objective of this research was to evaluate the effect of eCG on morphological quality and gene expression profile of cumulus-oocyte complexes (COC) recovered from anestrous cats. For this purpose, 3 experimental groups were made. Group 1 consisted of 11 oestrous cats (oestrous); Group 2 had 13 anestrous cats (anestrous); and Group 3 was made up of 11 anestrous cats treated with a single subcutaneous dose of 200 IU of eCG. In oestrous and anestrous groups the ovaries were obtained directly by ovariohysterectomy, whereas in the eCG group this was achieved 4 days after the dose injection. In all groups, each cat corresponded to an individual biological replicate, whereby the COC recovered from each cat were classified and processed separately for the experiments of gene expression analysis and in vitro maturation (IVM). The COC were collected by slicing of the ovaries and classified morphologically as grade I (excellent), grade II (good), grade III (fair), and grade IV (poor) quality. For gene expression analysis, pools of 8 to 10 grade I and II immature COC were made, resulting in 7 pools for each group. Quantitative RT-PCR was performed for gonadotrophin receptor genes (FSHR and LHCGR), FSH-induced genes (EGFR, EGR1, ESR2, and PTGS2), and genes related to oocyte competence (GDF9, BMP15, and GATM). The gene SDHA was used as the internal control. The total remaining proportion of grade I and II COC were used for IVM, and maturation rate was measured by visualisation of the first polar body. Statistical analysis was performed using the Kruskal–Wallis test. No differences were found in the total number of COC (mean ± standard deviation) recovered per cat among the oestrous (56.8 ± 20.5), anestrous (80.2 ± 35.2), and eCG groups (96.5 ± 62.0; P > 0.05). With respect to morphological quality of COC, the eCG group had a higher proportion of grade I COC (33.6 ± 11.0%) than the oestrous and anestrous groups (16.5 ± 8.7 and 8.9 ± 6.0%, respectively; P < 0.05). However, the anestrous group had a higher proportion of grade II COC (26.8 ± 6.4%) than the eCG group (21.1 ± 6.6%; P < 0.05). On the other hand, the eCG group had a lower proportion of grade III and IV COC (45.3 ± 12.8%) than the anestrous group (64.3 ± 9.1%; P < 0.05), without differences from the oestrous group (57.1 ± 12.0%; P > 0.05). Concerning to gene expression analysis, COC from the eCG group had a higher relative expression of FSHR, LHCGR, and EGFR than COC from the oestrous and anestrous group (P < 0.05). Furthermore, the COC from the eCG group had a higher relative expression of EGR1 than COC from the anestrous group and a higher expression of ESR2 than COC from the oestrous group (P < 0.05). However, COC from the eCG group had a lower relative expression of GATM and PTGS2 than COC from the oestrous group and a lower expression of GDF9 and BMP15 than COC from the anestrous group (P < 0.05). Although a higher number of oocytes with a first polar body could be seen in the eCG group after IVM, no significant differences in the maturation rate were found among the eCG (55.3 ± 13.2), oestrous (43.7 ± 11.1), and anestrous groups (47.9 ± 13.6; P > 0.05). In conclusion, the treatment with eCG improved the morphological quality of COC recovered from anestrous cats, which agrees with an increased relative expression of FSHR, LHCGR, EGFR, EGR1, and ESR2 and might be related to an enhanced competence of COC.

scholarly journals Fine quality evaluation of surrounding rock of an underground hydropower station

2020 ◽  
Vol 194 ◽  
pp. 05041
Author(s):  
Xiaojun Bao ◽  
Zhiyuan Wang ◽  
Shuai Ma ◽  
Gao Liu
Keyword(s):  
Quality Evaluation ◽  
Surrounding Rock ◽  
Classification Method ◽  
Hydropower Station ◽  
Rock Classification ◽  
Rock Quality ◽  
Grade Ii ◽  
Grade Iii

Using RMR surrounding rock classification method, the surrounding rock quality of underground main powerhouse of a hydropower station is evaluated with a small evaluation unit. The results of surrounding rock classification show that the surrounding rock of main powerhouse is mainly Grade III, and Grade II and IV surrounding rock develop intermittently with the depth of main powerhouse, and there is no Grade I or V surrounding rock distribution. Secondly, setting a smaller evaluation section is conducive to improve the accuracy of surrounding rock quality evaluation and better grasp the distribution of different grades of surrounding rock in the evaluation area.

Preliminary Results of Therapy with AM3 in Patients Undergoing Hematopoietic Stem Cell Transplantation.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 5370-5370
Author(s):  
Valle Recasens ◽  
Araceli Rubio-Martinez ◽  
Pilar Alfonso ◽  
Ignacio De Blas ◽  
Daniel Rubio-Felix ◽  
...  
Keyword(s):  
Immune System ◽  
Infectious Diseases ◽  
Acute Leukemia ◽  
Oral Mucositis ◽  
Hematopoietic Stem ◽  
Group A ◽  
Grade Ii ◽  
Group B ◽  
Autologous Hsct ◽  
Grade Iii

Abstract Introduction: AM3(Inmunoferon®), is a glycoconjugate of natural origin with immunomodulatory properties indicated in secondary immnunodeficiencies as well as coadjuvant treatment in neoplastic diseases with cellular immunity deficiency. Aim: To evaluate the influence of AM3 in the biological recovery of immune system in patients undergoing hematopoietic stem cells transplantation (HSCT)(autologous/allogenic). As well as to register the clinical incidences as intercurrent infections and mucositis from day of infusion hematopoietic cells to 90 days postransplantion in patients with and without AM3. Patients and methods: Group A (cases): Inclusion of 19 consecutive patients undergoing HSCT. April 2004 - June 2005. Inclusion criteria: age ≥ 18 years, first HSCT. and signed consent form. AM3 dose: 20mg/8h orally since infusion day to 90 days postransplantation. Group B (controls): 19 patients consecutively undergoing HSCT. from February 2003- March 2004. Biological evaluation of the immune system recovery: BCC, immunoproteins quantification: IgG, IgA, IgM, C3, C4, lymphoid populations distribution and activity of monocyte biomarkers (chitotriosidase, CCL18/PARC) on days −7, 0,+7,+14,+21,+28,+56,+90. Clinical evaluation and comparing cases/controls of incidence of mucositis severity evaluated according to WHO classification and microbiological documented infectious diseases. Results: The addition of AM3 as coadjuvant therapy in patients undergoing HSCT reduce significantly the percentage of oral mucositis (63.1% vs 89.5%) (p= 0.025) being those mucositis of less complexity in the first 2 weeks postransplantation. The number of infectious diseases was lower in patients under AM3 therapy (31.5% vs 47.4%) (p= 0.254) (Detailed in table). The recovery of the immune system evaluated by blood cells counts and immunobiomarkers showed a initial recuperation at day +14 in autologous HSCT and at day 21 for allogenic HSCT. The tolerance was satisfactory and only two patients needed discontinue therapy because of digestive intolerance. Wide studies must be performed in order to evaluate the benefit of this coadjuvant therapy. The inclusion of AM3 as a coadjuvant therapy in patients undergoing HSCT is associated with a significant minor percentage of oral mucositis. This work has been partially sponsored by a grant from FEHHA. Group A Group B Mean age (range) 44 (18–67) 48 (18–68) Females (%) 6 (31%) 7 (36,8%) Allogenic HSCT (M/F) 4 (21%) (3M/1F) 6 (31,5%) (5M/1F) Autologous HSCT(M/F) 15 (78,9%) (10H/5M) 13(68,4%) (7M/6F) Diagnosis Acute leukemia: 7 (37%), MM 7 (37%), MDS 2 (10%), Hodgkin disease 2nd remission 2 (11%), lymphoblastic lymphoma 1 (5%) Acute leukemia 4 (21%), MM 9 (47%), Hodgkin disease 2nd remission 2 (11%), NHL 4 (21%) Mucositis 12 (63%). Grade I: 1 patient (9%); grade II: 4 (33%); grade III: 4 (33%) 17 (89.5%). Grade II: 1 patient (6%); grade III: 6 (35%); grade IV: 9 (53%) Infectious diseases 6 (31,5%): S. Epidermidis 4 (21%), S. hominis 1 (5%), CMV 2 (10,5%), C. Albicans 1 (5%) 8 (42%): S. coagulase negative 7 (36,8%), S. viridans 1 (5.2%), S. epidermidis 1 (5.2%), Acinetobacter junii 1 (5.2%), Ochrobactrum anthropi 1 (5.2%), P. aeruginosa 1 (5.2%), C. difficile 1 (5.2%), ADN Herpes virus 6: 2 (10.5%), CMV 3 (15.7%), C. albicans 2 (10.5%), C. Krusei 1 (5.2%), P. carinii 1 (5.2%)

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