scholarly journals Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei)

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2669
Author(s):  
Priscila Silva de Almeida-Monteiro ◽  
Mayara Setúbal Oliveira Araújo ◽  
Romulo Roberto Ribeiro Pinheiro ◽  
Júlia Trugilio Lopes ◽  
Yasmim Maia Ferreira ◽  
...  
Keyword(s):  
Vitamin E ◽  
Vitamin C ◽  
Deleterious Effect ◽  
Membrane Integrity ◽  
Sperm Cells ◽  
Second Stage ◽  
Sperm Membrane ◽  
Two Stages

Seminal cryopreservation allows the long-term conservation of gametes of various species, including endangered species, such as Prochilodus brevis. However, the application of this biotechnology can cause damage to sperm cells, reducing seminal quality. Thus, we have sought substances that minimize the damage caused by this process, such as antioxidants. Thus, this study aimed to evaluate the association between two cryoprotectants and two vitamins, in different concentrations, on the quality of cryopreserved semen of P. brevis. For cryopreservation, the experiment was performed in two stages. In the first stage, the semen of 10 animals was submitted to six different freezing means, coming from the combination of 5% glucose, two cryoprotectants (Dimethyl sulfoxide [DMSO] or Methyl glycol) and two vitamins (C or E to 0.0001 mg) for cryopreservation. In the second stage, semen samples of eight animals were diluted in 5% glucose and the best cryoprotectant found in the first stage, associated with three different concentrations of vitamins C or E (0.01, 0.001, and 0.0001 mg). In both steps, the in natura and post-thawed samples were submitted to kinetic analysis, morphology, and sperm membrane integrity. The cryopreserved semen with DMSO presented significantly higher results (p < 0.05) than that frozen with Methyl glycol, regardless of the vitamin used. The morphologically normal spermatozoa rate was higher (p < 0.05) in the vitamin-containing samples, however, vitamin E reduced sperm motility rates, independent of the cryoprotectant used. As for vitamin concentrations, higher motility rates were obtained when cryopreserved semen with 0.01 and 0.0001 mg of any of the vitamins. However, the higher concentration had a deleterious effect on the spermatic morphology of P. brevis. Therefore, the glucose associated with DMSO and the lower concentration of vitamin C provides good quality for the post-thawed semen of P. brevis.

scholarly journals Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei)

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2669 ◽  
Author(s):  
Priscila Silva de Almeida-Monteiro ◽  
Mayara Setúbal Oliveira Araújo ◽  
Romulo Roberto Ribeiro Pinheiro ◽  
Júlia Trugilio Lopes ◽  
Yasmim Maia Ferreira ◽  
...  
Keyword(s):  
Vitamin E ◽  
Vitamin C ◽  
Deleterious Effect ◽  
Membrane Integrity ◽  
Sperm Cells ◽  
Second Stage ◽  
Sperm Membrane ◽  
Two Stages

Seminal cryopreservation allows the long-term conservation of gametes of various species, including endangered species, such as Prochilodus brevis. However, the application of this biotechnology can cause damage to sperm cells, reducing seminal quality. Thus, we have sought substances that minimize the damage caused by this process, such as antioxidants. Thus, this study aimed to evaluate the association between two cryoprotectants and two vitamins, in different concentrations, on the quality of cryopreserved semen of P. brevis. For cryopreservation, the experiment was performed in two stages. In the first stage, the semen of 10 animals was submitted to six different freezing means, coming from the combination of 5% glucose, two cryoprotectants (Dimethyl sulfoxide [DMSO] or Methyl glycol) and two vitamins (C or E to 0.0001 mg) for cryopreservation. In the second stage, semen samples of eight animals were diluted in 5% glucose and the best cryoprotectant found in the first stage, associated with three different concentrations of vitamins C or E (0.01, 0.001, and 0.0001 mg). In both steps, the in natura and post-thawed samples were submitted to kinetic analysis, morphology, and sperm membrane integrity. The cryopreserved semen with DMSO presented significantly higher results (p < 0.05) than that frozen with Methyl glycol, regardless of the vitamin used. The morphologically normal spermatozoa rate was higher (p < 0.05) in the vitamin-containing samples, however, vitamin E reduced sperm motility rates, independent of the cryoprotectant used. As for vitamin concentrations, higher motility rates were obtained when cryopreserved semen with 0.01 and 0.0001 mg of any of the vitamins. However, the higher concentration had a deleterious effect on the spermatic morphology of P. brevis. Therefore, the glucose associated with DMSO and the lower concentration of vitamin C provides good quality for the post-thawed semen of P. brevis.

scholarly journals Efektivitas Penambahan Vitamin E (alfa-Tokoferol) dalam Medium Pencucian Sperma dengan Sentrifugasi terhadap Kualitas Spermatozoa Sapi Brahman

2012 ◽  
Vol 12 (2) ◽  
pp. 7-13
Author(s):  
Dasrul Dasrul ◽  
Rasmaidar Rasmaidar ◽  
Abdul Harris
Keyword(s):  
Vitamin E ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Average Percentage ◽  
Treatment Groups ◽  
Frozen Semen ◽  
Brahman Cattle ◽  
Sperm Membrane ◽  
Better Than

Effect of vitamin E addition (alfa-tokoferol) into sperm washing medium by centrifuge on the quality of Brahman cattle spermatozoa ABSTRACT. The aims of study to determine the effectiveness of the addition of vitamin E in the washing medium by centrifugation on sperm quality Brahman cattle. frozen semen of Brahman cattle, divided into 4 treatment groups addition of vitamin E in the washing medium: 0.0gr/100 ml medium (K0), 0.1gr /100 ml medium (K1); 0.2gr/100 ml medium (K2) and 0.3 g / 100 ml medium (K4), each group was repeated 5 times. Examination of motility, viability and integrity of sperm membrane performed according to WHO standards. The data obtained were analyzed with one-way ANOVA and Duncan test. The average percentage of motility, viability and membrane integrity of spermatozoa in the addition of vitamin E were significantly different (P 0.05) compared to the control. Percentage of motility, viability and membrane integrity of spermatozoa in the group K2 significantly higher (P 0.05) compared with the group K3: K1 and K0. Percentage of motility, viability and sperm capacitation and sperm live on group K3 significantly higher (P 0.05) compared with the K1 and K0. While the percentage motility of spermatozoa in the group K1 higher were not significant (P 0.05) compared with the group K0. The addition of vitamin E in the medium on the process of washing spermatozoa Brahman cattle. The addition of vitamin E 0.2gr/100ml better than vitamin E 0.1gr/100ml and 0.3gr/100ml in maintaining the percentage of motility and live spermatozoa Brahman cattle.

scholarly journals Effects of long-term liquid commercial semen extender and storage time on the membrane quality of boar semen

2010 ◽  
Vol 55 (No. 4) ◽  
pp. 160-166 ◽  
Author(s):  
S. Frydrychová ◽  
J. Čeřovský ◽  
A. Lustyková ◽  
M. Rozkot
Keyword(s):  
Storage Time ◽  
Membrane Integrity ◽  
Simple Method ◽  
Laboratory Methods ◽  
Significant Difference ◽  
Sperm Membrane ◽  
Boar Semen ◽  
And Storage

The objective of this study was to assess the sperm membrane integrity in extended boar semen during storage time using specific spectrum laboratory methods. Boar semen was diluted with the long-term liquid commercial extenders Androhep (A), Androstar (AS), Androstar plus (AS<SUP>+</SUP>), LD and M III and was stored up to 96 h. The sperm membrane integrity was evaluated by motility, viable spermatozoa, short hypoosmotic swelling test (sHOST) and by the activity of the enzyme aspartate aminotransferase (AST). Negative changes in the quality of sperm membrane in relation to storage time were observed after 48 h for sHOST, after 72 h for viable spermatozoa and after 72 h for motility. The percentage of viable spermatozoa was decreased by 0.27% each hour. A statistically significant difference between extenders A and LD was observed in sHOST after 72 h and 96 h storage (<I>P</I> &lt; 0.05). The AST activity did not show any statistically significant differences in extenders and in storage time. In overall assessment Androhep was the best of the tested extenders, followed by AS, AS<SUP>+</SUP>, M III and LD in terms of motility, viable spermatozoa and sHOST. The correlations among laboratory methods were highly significant (<I>P</I> &lt; 0.001). In conclusion, the results documented that the sperm membrane integrity was statistically significantly affected by extenders and storage time (<I>P</I> &lt; 0.001). Boar semen quality was the best in extender A. sHOST is a very sensitive and relatively simple method for the assessment of sperm membrane integrity in diluted semen.

Acute Pancreatitis Has a Long-term Deleterious Effect on Physical Health Related Quality of Life

2017 ◽  
Vol 15 (9) ◽  
pp. 1435-1443.e2 ◽  
Author(s):  
Jorge D. Machicado ◽  
Amir Gougol ◽  
Kimberly Stello ◽  
Gong Tang ◽  
Yongseok Park ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Acute Pancreatitis ◽  
Physical Health ◽  
Deleterious Effect ◽  
Health Related Quality ◽  
Related Quality ◽  
Health Related

A novel two-stage method of plant seedlings classification based on deep learning

2021 ◽  
pp. 1-11
Author(s):  
Tianhong Dai ◽  
Shijie Cong ◽  
Jianping Huang ◽  
Yanwen Zhang ◽  
Xinwang Huang ◽  
...  
Keyword(s):  
Deep Learning ◽  
Learning Technology ◽  
Two Stage ◽  
Second Stage ◽  
Stage Classification ◽  
Different Types ◽  
Two Stages ◽  
Plant Seedlings

In agricultural production, weed removal is an important part of crop cultivation, but inevitably, other plants compete with crops for nutrients. Only by identifying and removing weeds can the quality of the harvest be guaranteed. Therefore, the distinction between weeds and crops is particularly important. Recently, deep learning technology has also been applied to the field of botany, and achieved good results. Convolutional neural networks are widely used in deep learning because of their excellent classification effects. The purpose of this article is to find a new method of plant seedling classification. This method includes two stages: image segmentation and image classification. The first stage is to use the improved U-Net to segment the dataset, and the second stage is to use six classification networks to classify the seedlings of the segmented dataset. The dataset used for the experiment contained 12 different types of plants, namely, 3 crops and 9 weeds. The model was evaluated by the multi-class statistical analysis of accuracy, recall, precision, and F1-score. The results show that the two-stage classification method combining the improved U-Net segmentation network and the classification network was more conducive to the classification of plant seedlings, and the classification accuracy reaches 97.7%.

scholarly journals Cost Effectual Distributed Cooperative Cluster based Communication Protocol in Wireless Sensor Network

2020 ◽  
Vol 8 (6) ◽  
pp. 5652-5656
Keyword(s):  
Communication Protocol ◽  
Wireless Sensor ◽  
Team Leaders ◽  
Great Strength ◽  
Second Stage ◽  
Remote Sensor ◽  
Channel Distortion ◽  
Two Stages ◽  
Intense Research

Theoretical energy recognition in remote sensor systems has received intense research interest in the late years. Radio variation, channel distortion, and blockage bring great strength and responsiveness to packets broadcast over a remote channel. A twin innovation is effective communication that can drastically increase the channel range and reduce transmission vigor consumption in disrupting channel. Growth in the direct range brings with it a reduced fault rate. In this paper, an acceptable correspondence method is proposed for each tab with active sending and receiving clusters. It consists of two stages, the precise routing phase, the selective and transmitting stage. In the routing phase, the basic route between the source and the sink hub is started. In the second stage, centers of fundamental development toward flattering team leaders select additional touch centers with minimal biomass costs from their surroundings, and then spread from bundle to cluster to the recently established endurance cluster. Reductions in error rate and regeneration are proven by the fact that malpractice funds become long-term obligation systems.

scholarly journals Assessment of quality in specific fractions of Large White Yorkshire boar semen

2021 ◽  
Vol 52 (2) ◽  
Author(s):  
K. G. Ambily ◽  
Malati Naik ◽  
Hiron M. Harshan ◽  
C. Jayakumar ◽  
M. P. Unnikrishnan ◽  
...  
Keyword(s):  
Cold Shock ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Membrane Cholesterol ◽  
Large White ◽  
Progressive Motility ◽  
Sperm Membrane ◽  
Boar Semen ◽  
Quality Assessments

Boar semen is voluminous and ejaculated as jets or fractions of pre-sperm, sperm rich (SRF) and post-sperm rich fractions. Recent studies have reported more resilient characteristics of sperm in initial portions of SRF towards cold shock and cryopreservation. The present study was conducted to assess the quality of specific fractions of SRF, namely, first 10mL of SRF (F1) and rest of SRF (F2) in Large white Yorkshire (LWY) boar semen. Ejaculates were collected using gloved-hand technique and were subjected to quality assessments of volume, pH, sperm progressive motility, concentration, plasma membrane integrity, abnormality, acrosome integrity and sperm membrane cholesterol. Upon statistical analysis, significant differences were noticed in volume, pH, sperm concentration and sperm membrane cholesterol between fractions of the ejaculate.

Towards a Renewed Vision of Imperial Authority

2019 ◽  
pp. 237-269
Author(s):  
Florin Leonte
Keyword(s):  
Fourteenth Century ◽  
Political Communication ◽  
The West ◽  
Substantial Body ◽  
Political Messages ◽  
Imperial Authority ◽  
Second Stage ◽  
Two Stages

The chapter discusses how Manuel Palaiologos’ texts reveal not only the emperor’s standpoints in his attempts to answer political challenges, but also a long-term imperial project that sought to establish a system of effective political communication by exhibiting his fatherly concern for his son and co-emperor. This project involved two stages with changing approaches. In the first stage, the emperor strengthened his connections with the literati and frequently chaired theatra. The letters and the dialogic mode of his text on marriage point to the fact that during the last decade of the fourteenth century, the emperor did not have at his disposal too many possibilities of circulating his political messages except for the rather informal meetings in the framework of theatra. In a second stage, which chronologically coincided with the years following the emperor’s return from the West, Manuel attempted to consolidate his ruling position by highlighting in the Foundations and the Orations that he appointed his son, John, as successor. In the absence of a more substantial body of court rhetoricians, the emperor undertook the role of a social-political commentator and accordingly put forward a personal discourse on imperial authority.

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