scholarly journals Effects of slow-release urea on in vitro rumen fermentation parameters, growth performance, nutrient digestibility and serum metabolites of beef cattle

2020 ◽  
Vol 41 (4) ◽  
pp. 1399
Author(s):  
Huan Liang ◽  
Erlong Zhao ◽  
Chunyan Feng ◽  
Jianfei Wang ◽  
Lanjiao Xu ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Soybean Meal ◽  
Dry Matter ◽  
Slow Release ◽  
Rumen Fermentation ◽  
Nutrient Digestibility ◽  
Serum Metabolites ◽  
Fermentation Parameters ◽  
Slow Release Urea

Two experiments were conducted to investigate the effects of slow-release urea (SRU) on in vitro rumen fermentation parameters, growth performances, nutrient digestibility, and serum metabolites of beef cattle. The single factor design was applied in both experiments. Three diets with different nitrogen sources including soybean meal (Control group), slow-release urea (SRU group), and common urea (Urea group) was designed (concentrate to forage ratio was 4:6). The diets were formulated to be isoenergetic and isonitrogenous, 75% of the soybean meal in the control diet was replaced by 1.41% SRU and 1.15% urea in SRU group and Urea group, respectively. In experiment 1, five healthy Jinjiang cattle (average body weight (BW) was 380 ± 17.1 kg) with permanent rumen fistulas were used in in vitro ruminal fermentation experiment. The results showed that supplementing SRU increased the dry matter degradation rate (DMD), digestible organic matter (DOM) and propionic acid concentration in cultivated fluid, and SRU supplementation decreased pH, NH3-N, total volatile fatty acid (TVFA), acetic acid, butyric acid concentration and microbial growth efficiency (MOEFF) in cultivated fluid. In experiment 2, eighteen Simmental crossbred cattle BW= 315 ± 5.2 kg) were stratified by BW and then assigned to the three groups to have equal BW among groups. The results showed that supplementing SRU reduced the average dry matter intake (ADMI), apparent digestibility of ether extract (EE), the activity of glutathione peroxidase (GSH-Px), the levels of IgG and IgA, and the production of thiiodothronine (T3) in serum, SRU supplementation increased the apparent digestibility of dry matter and organic matter (OM) and alanine aminotransferase (ALT) concentration in serum. These results indicated that some soybean meal could be replaced by SRU and urea in the production of beef cattle. In addition, compared with urea, SRU had a good sustained-release effect. The replacement of some soybean meal by SRU in the diet had no adverse impact on rumen fermentation, growth performance, and serum metabolites of beef cattle.

scholarly journals Slow-Release Urea as a Sustainable Alternative to Soybean Meal in Ruminant Nutrition

2021 ◽  
Vol 13 (5) ◽  
pp. 2464
Author(s):  
Saheed A. Salami ◽  
Maria Devant ◽  
Juha Apajalahti ◽  
Vaughn Holder ◽  
Sini Salomaa ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Soybean Meal ◽  
Dry Matter ◽  
Vegetable Protein ◽  
Slow Release ◽  
Protein Sources ◽  
Slow Release Urea ◽  
In Vitro Rumen Fermentation

Three experiments were conducted to evaluate the feasibility of using a commercial slow-release urea product (SRU; Optigen®, Alltech Inc., Nicholasville, KY, USA) as a partial replacement for vegetable protein sources in cattle diets. The first experiment was an in vitro rumen fermentation that evaluated the effect of replacing soybean meal (SBM) nitrogen with nitrogen from either SRU or free urea in diets varying in forage:concentrate ratios. The second experiment examined the effect of replacing SBM with SRU on in situ dry matter and nitrogen degradability in the rumen. In the third experiment, a feeding trial was conducted to evaluate the effect of replacing SBM (0% as-fed SRU) with 1% or 3% as-fed SRU on feed carbon footprint (CFP; total greenhouse gas emissions associated with the life cycle of feed raw materials) and the toxicity potential of SRU in growing beef cattle. Results showed that replacing SBM with SRU up to 1.3% did not negatively affect in vitro rumen fermentation parameters. Supplementing SRU favourably decreased ruminal accumulation of ammonia and lactic acid when compared to free urea. There was no significant effect on effective rumen degradability of dry matter and nitrogen when one-third of SBM was replaced by SRU in the in situ study. Compared with the 0% SRU diet, feed CFP decreased by 18% and 54% in 1% SRU and 3% SRU diets, respectively. Additionally, feeding up to 3% SRU diet to beef cattle did not affect health and intake, and blood hematological and biochemical indices were within the physiological range for healthy bulls, suggesting no indication of ammonia toxicity. Overall, these results indicate that SRU can be used as a sustainable alternative to partially replace vegetable protein sources in ruminant diets without compromising rumen function and health of ruminants.

scholarly journals Ruminal fermentation and digestion of cattle diets with total and partial replacement of soybean meal by a slow-release urea product

2019 ◽  
Vol 64 (No. 7) ◽  
pp. 294-301
Author(s):  
S Gonzalez-Munoz ◽  
J Sanchez ◽  
S Lopez-Aguirre ◽  
J Vicente ◽  
J Pinos-Rodriguez
Keyword(s):  
Soybean Meal ◽  
Degradation Rate ◽  
Slow Release ◽  
In Vitro Assay ◽  
Partial Replacement ◽  
Vitro Assay ◽  
Dietary Substitution ◽  
Slow Release Urea

One in vitro assay and one in vivo trial with ruminally cannulated Holstein steers were conducted to evaluate the effects of a dietary substitution of soybean meal by a urea and slow-release urea source of fermentation and degradation of diets for cattle. The experimental diets consisted of the total mixed rations defined as the control with soybean meal (SBM), U (urea), SRU (slow-release urea), and SRU+U+AA (0.42% + 0.42% + 1% amino acids methionine and lysine). The dietary substitution of SBM by U or SRU reduced (P < 0.05) the total gas production (V), microbial mass and degradation at 72 h incubation under the in vitro conditions, as well as the degradation rate (c) and the total volatile fatty acids (VFA) in the rumen of the steers; however, when the dietary substitution of SBM was by U+SRU+AA, those values did not decrease. In the steers, the dietary substitution of SBM by U and SRU reduced the ruminal degradation rate and the total VFA, and increased the ammonia N, but when SBM was substituted by U+SRU+AA in the diets, these changes were not observed. No advantage of SRU over U was found. The dietary substitution of SBM by U, SRU, U+SRU+AA did not modify the molar proportion of the VFA in the rumen nor were there changes in the nutrient digestion or excretion. Both the in vitro assay and the in vivo trial indicated that replacing SBM with U or SRU increases the ruminal ammonia N concentrations and reduces the degradation rate in the rumen, although those undesirable findings were not found when the SBM was replaced by U+SRU+AA. Therefore, it is feasible to replace the SBM with a combination of urea, slow-release urea, lysine and methionine in the diet for the ruminants.

scholarly journals Sunflower cake in diets for beef cattle: digestibility, kinetics and in vitro ruminal fermentation parameters

2018 ◽  
Vol 41 (1) ◽  
pp. 39492 ◽  
Author(s):  
Rafael Henrique de Tonissi e Buschinelli de Goes ◽  
Luiz Henrique Xavier da Silva ◽  
Tatiana García Díaz ◽  
Antonio Ferriani Branco ◽  
Ana Lúcia Teodoro ◽  
...  
Keyword(s):  
Organic Matter ◽  
Beef Cattle ◽  
Soybean Meal ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Fermentation Products ◽  
Sunflower Cake

 The objective of this study was to evaluate the effect of the inclusion of sunflower cake replacing soybean meal in beef cattle diets on the in vitro digestibility of dry matter (IVDDM), organic matter (IVDOM), crude protein (IVDCP) and the ruminal fermentation kinetics and parameters. The experiment was analyzed according to a completely randomized design. The treatments consisted of four levels of sunflower cake, 0, 200, 400, 600 g kg-1, replacing soybean meal in the concentrate of beef cattle diets. The coefficients of IVDDM, IVDOM and IVDCP presented a quadratic effect with the addition of sunflower cake. The soluble fraction (fraction B) degradation rate and total gas production decreased linearly with the inclusion of sunflower cake. Values of pH in ruminal fluid were higher for levels 0, 200 and 600 g kg-1 sunflower cake. Sunflower cake can replace soybean meal by up to 280 g kg-1 in the concentrate of beef cattle diets, improving the in vitro digestibility of dry matter organic, matter and crude protein. Levels above 400 g kg-1 reduce ruminal digestion rate, digestibility and release of final fermentation products. 

scholarly journals Effects of Slow Release Urea Supplementation of Sheep Protein Source Feed Protected with Condensed Tannin from Leucaena on Protein Degradation in Rumen and Post-rumen In Vitro

2018 ◽  
Vol 19 (2) ◽  
pp. 119
Author(s):  
Efka Aris Rimbawanto ◽  
Sri Suhermiyati ◽  
Bambang Hartoyo
Keyword(s):  
Protein Synthesis ◽  
Soybean Meal ◽  
Slow Release ◽  
Condensed Tannin ◽  
Protein Source ◽  
Fermentation Time ◽  
Residual Nitrogen ◽  
Slow Release Urea ◽  
Cassava Waste

The objectives of the research were to supply nitrogen for protein synthesis of ruminal microbe from slow release urea and to supply post-rumen protein from soybean meal protected with condensed tannin (CT) from crude Leucaena leaves extract. In Experiment 1, slow release urea (SRU) was made by extrusion of cassava waste-urea, tapioca meal-urea and cassava meal-urea. Evaluation of SRU properties was based on residual nitrogen concentration and ruminal fermentation products (total volatile fatty acid and ammonia-nitrogen) in vitro. In Experiment 2, soybean meal protected with CT from crude Leucaena leaves extract as much as 1,68 g tannin/100 g DM soybean meal. SRU that was selected from Experiment 1 was used as the supplement in sheep ration whose protein source was protected with CT. SRU supplement in basal rations was 0, 6.36, 12.75, 19.11% DM. In vitro result in Experiment 1, showed that SRU of cassava waste, tapioca meal and cassava meal were different (P<0,01) on residual nitrogen content, total VFA, ammonia-N, and fermentation time. The optimum SRU obtained from cassava waste by inhibiting nitrogen hydrolysis up to 14 h. In Experiment 2, SRU supplementation of cassava waste on basal ration whose protein source was protected with CT increased dry matter (DM) degradability (P<0,01), total VFA (P<0,01), ammonia-N (P<0,01), and did not affect crude protein (CP) ruminal degradation. Post-ruminal degradability of DM and CP increased (P<0.01) in line with the increasing supplement, and reached the optimum level at 12,75 – 19,11% DM. In conclusion, cassava waste was a potent SRU through extrusion process and could be harnessed as SRU supplement in ration with protein source protected with condensed tannin to improve ruminal microbe protein synthesis.

scholarly journals Soybean meal replaced by slow release urea in finishing diets for beef cattle

2014 ◽  
Vol 165 ◽  
pp. 51-60 ◽  
Author(s):  
P.D.B. Benedeti ◽  
P.V.R. Paulino ◽  
M.I. Marcondes ◽  
S.C. Valadares Filho ◽  
T.S. Martins ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Soybean Meal ◽  
Slow Release ◽  
Finishing Diets ◽  
Slow Release Urea

scholarly journals Effects of garlic supplementation on in vitro nutrient digestibility, rumen fermentation, and gas production

2021 ◽  
Vol 51 (2) ◽  
pp. 271-279
Author(s):  
M.R. Kekana ◽  
D. Luseba ◽  
M.C. Muyu
Keyword(s):  
Fatty Acids ◽  
Crude Protein ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Garlic Powder

Garlic contains secondary metabolites with antimicrobial properties that can alter nutrient digestibility and rumen fermentation, similar to other antimicrobial products. The objectives of the study were to evaluate the effects of garlic powder and garlic juice on in vitro nutrient digestibility, rumen fermentation, and gas production. The treatments consisted of control with no additives, garlic powder, and garlic juice at 0.5 ml and 1 ml. The digestibility of dry matter, crude protein and neutral detergent fibre were determined after 48 hours incubation. Rumen ammonia nitrogen and volatile fatty acids were determined at 12 hours and 24 hours incubation. The cumulative gas production was recorded periodically over 48 hours. The in vitro dry matter disappearance decreased with 1 ml of garlic juice compared with control. The crude protein degradability in garlic powder and garlic juice was lower than in control. Volatile fatty acids increased in all treatments. Individual volatile fatty acids were significantly different, especially propionate, whereas the acetate to propionate ratio was reduced by garlic juice, and ammonia nitrogen was reduced by garlic powder and 0.5 ml of garlic juice. The cumulative gas production increased significantly with both levels of garlic juice. The addition of garlic juice at 0.5 mL/100 ml could enhance the production of propionate, and reduce the acetate to propionate ratio, implying that the supply of hydrogen for methanogens was limited.

scholarly journals Effects of wilting and additives on the ensiling quality and in vitro rumen fermentation characteristics of sudangrass silage

2021 ◽  
Vol 34 (1) ◽  
pp. 56-65
Author(s):  
Jiang Chun Wan ◽  
Kai Yun Xie ◽  
Yu Xiang Wang ◽  
Li Liu ◽  
Zhu Yu ◽  
...  
Keyword(s):  
Production Rate ◽  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Ruminal Fermentation ◽  
Fresh Matter ◽  
Positive Effects

Objective: This study was conducted to investigate the effects of molasses and <i>Lactobacillus plantarum</i> on the ensiling quality and <i>in vitro</i> rumen fermentation of sudangrass silage prepared with or without wilting.Methods: The ensiling experiment, measured with 3 replicates, was carried out according to a 2×4 (wilted stages×additives) factorial treatment structure. Dry matter of the fresh (210 g/kg fresh matter) or wilted (305 g/kg fresh matter) sudangrass were ensiled (packed into 5.0-L plastic jars) without additive (control) or with molasses (M), <i>Lactobacillus plantarum</i> (LP), or molasses + <i>Lactobacillus plantarum</i> (M+LP). After 60 days of ensiling, the silages were analyzed for the chemical, fermentation, and <i>in vitro</i> characteristics.Results: After 60 days of ensiling, the fermentation parameters were affected by wilted, the additives and the interactions of wilted with the additives (p<0.05). The M+LP treatment at wilted had higher lactic acid levels and V-score (p<0.05) but lower pH values and butyric acid concentrations than the other treatments. In comparison with sudangrass before ensiling, after ensiling had lower dry matter and higher non-fibrous carbohydrate. The <i>in vitro</i> gas production, <i>in vitro</i> dry matter digestibility, <i>in vitro</i> crude protein digestibility, and <i>in vitro</i> acid fiber detergent digestibility changed under the effects of the additives. Significant interactions were observed between wilted and the additives in terms of <i>in vitro</i> gas production at 48 h, asymptotic gas production, gas production rate, half time, and the average gas production rate. The total volatile fatty acid levels in the additive treatments were higher than those in the control.Conclusion: Wilting and supplementation with molasses and <i>Lactobacillus plantarum</i> had the ability to improve the ensiling quality and <i>in vitro</i> nutrient digestibility of sudangrass silage. The M+LP treatment at wilted exhibited the strongest positive effects on silage quality and <i>in vitro</i> ruminal fermentation characteristics.

PSXIV-1 In vitro evaluation of methane mitigation using monensin and ammonium nitrate in beef cattle diets

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 471-472
Author(s):  
Ana Paula Tarozo ◽  
Annelise Aila G Gomes Lobo ◽  
Yuli Andrea A Peña Bermudez ◽  
Danny Alexander Rojas Moreno ◽  
Rafaela Zuliani Spalato ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Ammonium Nitrate ◽  
Methane Production ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Feed Additives ◽  
Control Treatment ◽  
Fermentation Kinetics ◽  
Fermentation Parameters

Abstract Currently, the use of feed additives appears as an alternative in reducing the environmental impact of animal agriculture, reducing the emission of greenhouse gases and increasing the acceptability of exports in international trade. Thus, the objective of the present study was to evaluate the in vitro rumen fermentation parameters by adding 4.5% ammonium nitrate and 30 ppm of the additive sodium monensin to beef cattle diets, searching for the best alternative to mitigate methane production. The experiment was performed in an in vitro gas production system, and the fermentation kinetics, methanogenesis and short-chain fatty acid (SCFA) production were studied. Regarding methanogenesis, it was observed that the diet with ammonium nitrate showed higher in vitro degradability in DM (P = 0.017) and lower methane production (in ml/g of DM; P = 0.0088), compared to the diet with sodium monensin. Considering the fermentation kinetics, it can be stated that acetate production in molar (%) was lower in control and monensin diets, and higher in nitrate and nitrate + monensin diets (P &lt; 0.0001). It is concluded that both treatments ammonium nitrate + sodium monensin and ammonium nitrate alone have mitigating effect on methane emission, when compared to the control treatment. However, ammonium nitrate is more effective in this regard, producing less methane in vitro and having no negative effect on rumen fermentation parameters.

scholarly journals Optimizing beef cattle performance using rumen-protected lysine supplementation in diets balanced for predicted amino acid and effective energy requirement

2017 ◽  
Author(s):  
◽  
Jacquelyn Prestegaard
Keyword(s):  
Beef Cattle ◽  
Continuous Culture ◽  
Soybean Meal ◽  
Dry Matter ◽  
Energy Requirement ◽  
Latin Square ◽  
Negative Control ◽  
Effective Energy ◽  
Dietary Treatments

The objectives of these experiments were to determine the bypass value of rumen-protected lysine and performance responses of beef cattle fed encapsulated lysine. During experiment 1 singe-flow continuous culture fermenters were fed a Lys-deficient control (CON), a Lys-sufficient diet supplemented with rumen-protected soybean meal (RPSBM; AminoPlus, Ag Processing Inc., Omaha, NE), or a Lys-sufficient diet containing a commercially available rumen-protected Lys product (RPLYS; USA Lysine, Kemin Industries Inc., Des Moines, IA). Results from continuous culture showed greater degradation of RPLYS than RPSBM and CON. During Experiment 2, an in vitro dry matter digestibility study showed DM degradation was 23 percent greater for pure USA Lysine than another encapsulated lysine product (AjiPro 2G, Ajinomoto Heartland, Inc., Omaha, NE). The next objective was to test if AjiPro would increase plasma Lys levels in vivo (and therefore indicate its successful ruminal bypass and small intestinal absorption). Experiment 3 involved a 3 x 3 Latin Square study conducted on cannulated crossbred steers fed a combination of rumen-protected soybean meal and two increased levels of the rumen-protected product, where it was fed to meet 100 percent (AJ100) and 150 percent (AJ150) absorbable AA to effective energy (EE) ratio. Plasma Lys levels were greater when steers were fed diets containing AjiPro in comparison to when they were fed a negative control (NEGCON) which did not contain the product and was deficient in absorbable Lys. From both the in vitro dry matter degradation experiment and the 3 x 3 Latin Square study, we determined AjiPro to be an effective source of bypass Lys, and consequently used it to conduct a growing through finishing study. During Experiment 4, we evaluated steer performance when fed diets balanced for predicted Lys requirement to EE ratio through its supplementation in several dietary treatments. Control treatments included a negative control (NEGCON) that was deficient in absorbable Lys and contained no rumen-protected products; and a positive control (POSCON) where rumen-protected soybean meal was used to balance absorbable AA to EE ratio. Three additional dietary treatments included similar amounts of rumen-protected soybean meal and incremental amounts of AjiPro formulated to provide 50 percent (AJ50), 100 percent (AJ100), or 150 percent (AJ150) of the absorbable Lys provided by POSCON. Starting on d 151 of the growth study, steers were weighed on 2 consecutive days every 14 d and assigned a final BW when no longer profitable (defined as when cost of gain exceeded value of gain). Steers remained profitable for greater days for NEGCON, POSCON and AJ100 than AJ150. Steers consuming POSCON had lesser ADG (kg/d) than all other treatments during the early finishing phase (d 75 to 112). However, steer ADG (kg/d) during late finishing (d 112 to 179) was greater for steers fed diets optimized for Lys requirement (POSCON and AJ100) than all other treatments. Between d 112 to 179, POSCON had greater G:F than all other treatments but did not differ from AJ100. When encapsulated Lys was under or over-supplemented, finishing steers became less profitable sooner. Feed efficiency increased with use of rumen-protected products during late finishing (d 112 to 179) in diets formulated to meet Lys requirement, but this did not impact overall steer performance from growing through finishing. A more accurate understanding of steer AA requirement and subsequent AA metabolism will allow more precise and effective use of rumenprotected products.

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