scholarly journals Nutrient content and accumulation in sugarcane under mineral fertilization and high doses of vinasse

2021 ◽  
Vol 42 (2) ◽  
pp. 565-582
Author(s):  
Monalisa Soares Costa ◽  
◽  
Mário Monteiro Rolim ◽  
Gerônimo Ferreira da Silva ◽  
Djalma Euzébio Simões Neto ◽  
...  
Keyword(s):  
Aerial Part ◽  
Mineral Fertilizer ◽  
Nutrient Content ◽  
Additional Treatment ◽  
High Dose ◽  
Mineral Fertilization ◽  
Initial Development ◽  
Dose Limits ◽  
Calcium Magnesium ◽  
High Doses

Vinasse, a waste from ethanol production, has been widely used as a means of fertigation in sugarcane. However, high dose limits application at and its effects on nutrient absorption and accumulation by the crop are not known. The aim of this study was to evaluate and compare, during the initial development of sugarcane, the effect of mineral fertilization and high doses of vinasse on the macronutrient and sodium contents of the top visible dewlap (TVD) leaf and their respective accumulations in the aerial part of the crop. The experiment was carried out in 100-dm3 pots filled with Argisol. The treatments consisted of five doses of vinasse (0, 150, 300, 600 and 1200 m3 ha-1) and an additional treatment with no vinasse and mineral fertilizer (500 kg ha-1 of the NPK 14-24-18 formulation). The levels of nitrogen, phosphorus, potassium, calcium, magnesium and sodium were evaluated in the TVD leaf at 120 days after planting and their accumulation in the aerial part of the plant at 210 days. Data were subjected to analysis of variance and compared using regression analysis and orthogonal contrasts. The vinasse doses applied did not adequately nourish the crop in macronutrients. Mineral fertilization provided higher N and P levels. The highest doses of vinasse provided higher levels of K, Ca and Mg and larger accumulations of N, P, K and Na, but were detrimental to the accumulation of Ca. The descending order of accumulation of macronutrients and sodium with mineral fertilization was P> Ca> N> K> Mg> Na. For the application of vinasse, the descending order was K> P> Ca> N> Na> Mg. In the absence of mineral fertilization and vinasse, the descending order was P> Ca> K> N> Mg> Na.

scholarly journals Productivity of lettuce in greenhouse as affected by humic and fulvic acids application in association to mineral fertilizer

2021 ◽  
Vol 39 (4) ◽  
pp. 444-450
Author(s):  
Ana Cristina Lüdtke ◽  
Deborah P Dick ◽  
Luiza Morosino ◽  
Vicente Kraemer
Keyword(s):  
Mineral Fertilizer ◽  
Nutrient Content ◽  
Fulvic Acids ◽  
Vegetable Crops ◽  
Humic And Fulvic Acids ◽  
Mineral Fertilization ◽  
Agronomic Efficiency ◽  
Combined Application ◽  
Nutritional Effect ◽  
Aerial Portion

ABSTRACT Humic substances (HS) have proved to be effective in improving the productivity of vegetable crops. We examined the effect of commercial biostimulants rich in humic (HA) and fulvic acids (FA) obtained from leonardite on agronomic efficiency and nutrient content in the leaves of lettuce (Lactuca sativa). A greenhouse experiment was carried out employing 2.5-L pots containing Ultisol samples that were supplied with commercial biostimulants rich in HA and FA, alone and in combination with NPK mineral fertilizer. The application of HS biostimulants associated to mineral fertilization to the lettuce plants provided the greatest values of the studied variables, namely: plant height and diameter, leaf number per plant and root length. The combination of FA biostimulants and mineral fertilization increased the lettuce productivity relative to the inorganic fertilizer alone. The combined application of rich biostimulants FA and HA with NPK fertilizes influenced K and Fe uptake by the plants, and their accumulation in the aerial portion. Our results suggest that HS products potentialize the nutritional effect of the mineral fertilizer, when applied in combination.

scholarly journals DESENVOLVIMENTO DE CULTIVARES DE SOJA APÓS INOCULAÇÃO DE ESTIRPES DE Bacillus subtilis

Nativa ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 126
Author(s):  
Letícia Carolina Costa ◽  
Renan Francisco Rimoldi Tavanti ◽  
Tauan Rimoldi Tavanti ◽  
Cassiano Spaziani Pereira
Keyword(s):  
Bacillus Subtilis ◽  
Glycine Max ◽  
Aerial Part ◽  
Crop Productivity ◽  
Additional Treatment ◽  
Subtilis Strain ◽  
Initial Development ◽  
Soybean Cultivars ◽  
Leaf Chlorophyll ◽  
Glycine Max L

O uso de bactérias diasotróficas na agricultura pode promover incrementos de produtividade das culturas. Este estudo teve por objetivo avaliar o efeito de diferentes concentrações de inoculantes a base de rizobactérias Bacillus subtilis no desenvolvimento inicial da cultura soja. Foram conduzidos dois experimentos com variedades comerciais de soja, M8210 e TMG132, avaliadas aos 15, 30 e 45 dias após semeadura (DAS). Em cada experimento, aplicou-se dois produtos no tratamento de sementes, um contendo a bactéria Bacillus subtilis estirpe pant001 nas concentrações de 0, 2, 4, 6 e 8 mL kg-1, e o tratamento adicional com um fungicida protetor comercial, que também possui Bacillus subtilis estirpe QST713 na sua formulação, na dose recomendada de 2 mL kg-1. Avaliou-se o índice Spad de clorofila de folhas do terço superior, médio e inferior do dossel aos 15, 30 e 45 DAS, a altura de plantas, fitomassa fresca e seca da parte aérea das plantas, fitomassa fresca e seca de raízes e volume de raízes aos 30 e 45 DAS. Os resultados indicaram aumento do índice Spad de clorofila das folhas de ambas as variedades com aumento das doses de Bacillus subtillis pant001, porém a inoculação não surtiu efeito sobre os atributos da parte aérea das plantas. Na variedade TMG132, houve incrementos na massa fresca de parte aérea após os 30 DAS e volume de raízes aos 45 DAS.Palavras-chave: Glycine max L., rizobactérias, tratamento de sementes. DEVELOPMENT OF SOYBEAN CULTIVARS AFTER INOCULATION WITH STRAINS OF Bacillus subtilis ABSTRACT: Diasotrophic bacteria in agriculture can promote increases in crop productivity. This study aimed to evaluate the effect of different concentrations of Bacillus subtilis rhizobacteria inoculants on the initial development of two soybean cultivars. Two experiments were conducted with commercial soybean varieties, M8210 and TMG132, evaluated at 15, 30 and 45 days after sowing (DAS). In each experiment, two products were applied in the treatment of seeds, a Bacillus subtilis strain pant001 at concentrations of 0, 2, 4, 6 and 8 mL kg-1, and the additional treatment with a commercial protective fungicide, which also has Bacillus subtilis strain QST713 in its formulation at the recommended dose of 2 mL kg-1. The leaf chlorophyll content index of the upper, middle and lower canopy was evaluated at 15, 30 and 45 DAS, height of plants, fresh and dry mass of the aerial part of the plants, fresh and dry root mass and volume roots at 30 and 45 DAS. The results indicated an increase in the Spad index of leaf chlorophyll of both varieties with increasing doses of Bacillus subtillis pant001, but the inoculation had no effect on the attributes of the aerial part of the plants. In the TMG132 variety, there was an increase in fresh shoot mass after 30 DAS and root volume at 45 DAS.Keywords: Glycine max L., rhizobacteria, seed treatment.

The Influence of Serotonin on Clot Retraction and the Thrombelastogram

1958 ◽  
Vol 02 (01/02) ◽  
pp. 111-124 ◽  
Author(s):  
E Deutsch ◽  
K Martiny
Keyword(s):  
Human Plasma ◽  
Human Platelet ◽  
Platelet Rich Plasma ◽  
Specific Effect ◽  
High Dose ◽  
Clot Retraction ◽  
Platelet Counts ◽  
Essential Value ◽  
High Doses ◽  
Free Plasma

Summary1. Normal platelets are necessary for induction of normal clot retraction.2. Serotonin does not induce retraction in human platelet-free plasma-clots or enhance clot firmness as measured in the coagulogram.3. Serotonin does not improve clot retraction or firmness in plasma clots with sub-optimal platelet counts.4. Methylserotonin inhibits clot retraction of platelet-rich plasma to a certain extent in moderate doses, whereas, high doses are ineffective. BOL 148 has a similar, but less significant action. There is a possibility that these effects are specific antiserotonin-effects.5. LSD 25 was ineffective in all concentrations used.6. Largactil and reserpin inhibit retraction in high doses. There seems to be a non specific effect caused by the high dose.7. Reserpine does not release a retraction-inducing agent from the platelets, which could be detected in the centrifuged platelet-free plasma used for the incubation.8. Serotonin does not replace the retraction-cofactor of Hartert, or the dialyzable factor of Lüscher in synthetic clotting substrates.9. Serotonin is of no essential value in inducing normal retraction of human plasma clots.

Estimation of the productivity and nutritionality of forage mass of new varieties of Medicago lupulina

2020 ◽  
Author(s):  
A.V. Vorsheva ◽  
◽  
G.V. Stepanova
Keyword(s):  
Vitamin D ◽  
Ascorbic Acid ◽  
Aerial Part ◽  
Nutrient Content ◽  
Calcium Salts ◽  
Green Mass ◽  
Medicago Lupulina ◽  
High Nutrient ◽  
New Varieties

Medicago has a high nutrient content, and the aerial part of Medicago lupulina contains saponins, tannins, estrogenic substances and calcium salts. The leaves of this plant contain carotene, vitamin D and ascorbic acid. Medicago lupulina can develop a significant green mass, has low pubescence, thin stems and provides a delicate forage. The article presents the results of assessing the productivity and feeding qualities of new varieties ofMedicago lupulina

Influence of β-D-mannuronic acid, as a new member of non-steroidal anti-inflammatory drugs family, on expression pattern of chemokines and their receptors in rheumatoid arthritis

2019 ◽  
Vol 16 ◽  
Author(s):  
Mona Aslani ◽  
Arman Ahmadzadeh ◽  
Zahra Aghazadeh ◽  
Majid Zaki-Dizaji ◽  
Laleh Sharifi ◽  
...  
Keyword(s):  
Cell Surface ◽  
Mrna Expression ◽  
Surface Expression ◽  
Phase Iii ◽  
Cell Surface Expression ◽  
Optimum Dose ◽  
High Dose ◽  
Mannuronic Acid ◽  
Anti Inflammatory ◽  
High Doses

Background: : Based on the encouraging results of phase III clinical trial of β-D-mannuronic acid (M2000) (as a new anti-inflammatory drug) in patients with RA, in this study, we aimed to evaluate the effects of this drug on the expression of chemokines and their receptors in PBMCs of RA patients. Methods:: PBMCs of RA patients and healthy controls were separated and the patients' cells were treated with low, moderate and high doses (5, 25 and 50 μg/mL) of M2000 and optimum dose (1 μg/mL) of diclofenac, as a control in RPMI-1640 medium. Real-time PCR was used for evaluating the mRNA expression of CXCR3, CXCR4, CCR2, CCR5 and CCL2/MCP-1. Cell surface expression of CCR2 was investigated using flow cytometry. Results:: CCR5 mRNA expression reduced significantly, after treatment of the patients' cells with all three doses of M2000 and optimum dose of diclofenac. CXCR3 mRNA expression down-regulated significantly followed by treatment of these cells with moderate and high doses of M2000 and optimum dose of diclofenac. CXCR4 mRNA expression declined significantly after treatment of these cells with moderate and high doses of M2000. CCL2 mRNA expression significantly reduced only followed by treatment of these cells with high dose of M2000, whereas, mRNA and cell surface expressions of CCR2 diminished significantly followed by treatment of these cells with high dose of M2000 and optimum dose of diclofenac. Conclusion:: According to our results, M2000 through the down-regulation of chemokines and their receptors may restrict the infiltration of immune cells into the synovium.

scholarly journals Reduced dose folinic acid rescue after rapid high-dose methotrexate clearance is not associated with increased toxicity in a pediatric cohort

2021 ◽  
Author(s):  
Riitta Niinimäki ◽  
Henri Aarnivala ◽  
Joanna Banerjee ◽  
Tytti Pokka ◽  
Kaisa Vepsäläinen ◽  
...  
Keyword(s):  
Folinic Acid ◽  
Lymphoblastic Leukemia ◽  
High Dose ◽  
Optimal Dosage ◽  
High Dose Methotrexate ◽  
Reduced Dose ◽  
Dose Methotrexate ◽  
Antileukemic Effect ◽  
Folinic Acid Rescue ◽  
High Doses

Abstract Purpose Low doses of folinic acid (FA) rescue after high-dose methotrexate (HD-MTX) have been associated with increased toxicity, whereas high doses may be related to a decreased antileukemic effect. The optimal dosage and duration of FA rescue remain controversial. This study was designed to investigate, whether a shorter duration of FA rescue in the setting of rapid HD-MTX clearance is associated with increased toxicity. Methods We reviewed the files of 44 children receiving a total of 350 HD-MTX courses during treatment for acute lymphoblastic leukemia according to the NOPHO ALL-2000 protocol. Following a 5 g/m2 HD-MTX infusion, pharmacokinetically guided FA rescue commenced at hour 42. As per local guidelines, the patients received only one or two 15 mg/m2 doses of FA in the case of rapid MTX clearance (serum MTX ≤ 0.2 μmol/L at hour 42 or hour 48, respectively). Data on MTX clearance, FA dosing, inpatient time, and toxicities were collected. Results Rapid MTX clearance was observed in 181 courses (51.7%). There was no difference in the steady-state MTX concentration, nephrotoxicity, hepatotoxicity, neutropenic fever, or neurotoxicity between courses followed by rapid MTX clearance and those without. One or two doses of FA after rapid MTX clearance resulted in a 7.8-h shorter inpatient time than if a minimum of three doses of FA would have been given. Conclusion A pharmacokinetically guided FA rescue of one or two 15 mg/m2 doses of FA following HD-MTX courses with rapid MTX clearance results in a shorter hospitalization without an increase in toxic effects.

scholarly journals AB0601 RAPID AND SUSTAINED EFFICACY OF AN INDUCTION TREATMENT WITH A TRIPLE THERAPY INCLUDING HIGH-DOSE INTRAVENOUS IMMUNOGLOBULINS, METHOTREXATE AND GLUCOCORTICOIDS IN ANTI-3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE MYOPATHY

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1597.1-1597
Author(s):  
E. Treppo ◽  
M. Infantino ◽  
M. Benucci ◽  
V. Ravagnani ◽  
B. Palterer ◽  
...  
Keyword(s):  
Triple Therapy ◽  
Antibody Titer ◽  
Intravenous Immunoglobulins ◽  
High Dose ◽  
Sustained Remission ◽  
Red Rice ◽  
Gradual Improvement ◽  
High Doses ◽  
High Dose Ivig

Background:Anti-3-hydroxy-3-methylglutaryl-coenzime A reductase (HMGCR) myopathy is a new entity, which has been clearly associated to statin use, even if it can be diagnosed in patients without a history of exposure to statin or even in the childhood (1).Objectives:The aim of the study is to describe the efficacy of a triple therapy regimen consisting in high-doses of intravenous immunoglobulins (IVIG), methotrexate (MTX), and glucocorticoids (GC) in 16 patients with Anti-HMGCR myopathy enrolled in 6 specialized centres.Methods:A total of 16 patients with anti-HMGCR myopathy (7 females; 9 males) were collected. Mean (±standard deviation) age at the onset of disease was 72.4±10.3 years old. All patients were diagnosed having anti-HMGCR myopathy [anti-HMGCR antibodies were measured by chemiluminescence assay (BioFlash, Inova, CA)] (2). Median follow-up was 29.5 months (interquartile range: 15.75-60 months). Anti-HMGCR antibodies were available in the follow-up in 8/16 patients.Results:Thirteen out of 16 patients (81.3%) had been exposed to statin (1/13 to red rice), 3/16 (18.7%) were not exposed. As induction therapy, 11/16 patients have been treated with triple therapy (high-dose IVIG, MTX and GC), 2/16 with double therapy (high-dose IVIG and GC), 2/16 have been treated with GC alone, the patient exposed to red rice resolved only with red rice suspension. Clinical remission and normalization of CPK values within month +24 were obtained in all the patients. All the patients were in remission at the last follow-up. Gradual improvement started soon from the first month, and among the 13 patients treated with an aggressive immunosuppresssive therapy including IVIG (13/13), GC (13/13) and methotrexate (11/13), 9/13 normalized the CPK value within 6 months. Clinical and laboratory response was accompanied by significant decrease or normalization of the anti-HMGCR antibody titer. All the patients were either not taking GC (56.3%), or were taking low doses of GC (43.7%) at the last follow-up. Four patients had stopped GC within 6 months. No serious side effects were recorded. After persistent remission, a maintenance immunosuppressive therapy was then administered. Only 3 relapses in 3 different cases were recorded, all of them during drug-free remission in long-term follow-up. Reinduction was again effective in all.Conclusion:Anti-HMGCR myopathy is a rare and serious myopathy which usually affects older people during statin treatment. After statin suspension, a rapid and sustained remission can be achieved by induction with a triple aggressive therapy consisting in medium-to high doses of GC, high-dose IVIG, and MTX (3). GC should be tapered as soon as possible. Relapse appears infrequent during maintenance treatment. Monitoring anti-HMGCR antibody titer may be clinically relevant.References:[1]AL Mammen et al. N Engl J Med. 2016;374:664-9[2]Musset L et al. Autoimmun Rev. 2016;15:983-93.[3]Aggarwal A et al. Scand J Rheumatol. 2019; 1-7.Acknowledgments:We thank MD Francesca Grosso and MD Valentina Mecheri from the University of Florence, MD Angela Zuppa and MD Chiara De Michelis, from San Martino Hospital, Genova, for their valued collaboration in data collectionDisclosure of Interests:Elena Treppo: None declared, Maria Infantino: None declared, Maurizio Benucci: None declared, Viviana Ravagnani: None declared, Boaz Palterer: None declared, Marina Grandis: None declared, Martina Fabris: None declared, Paola Tomietto: None declared, Mariangela Manfredi: None declared, Arianna Sonaglia: None declared, Maria Grazia Giudizi: None declared, Francesca Ligobbi: None declared, Daniele Cammelli: None declared, Paola Parronchi: None declared, Salvatore De Vita Consultant of: Roche, GSK, Speakers bureau: Roche, GSK, Novartis, Luca Quartuccio Consultant of: Abbvie, Bristol, Speakers bureau: Abbvie, Pfizer

Epidural Clonidine or Bupivacaine as the Sole Analgesic Agent during and after Abdominal Surgery 

1999 ◽  
Vol 90 (5) ◽  
pp. 1354-1362 ◽  
Author(s):  
Marc De Kock ◽  
Philippe Gautier ◽  
Athanassia Pavlopoulou ◽  
Marc Jonniaux ◽  
Patricia Lavand'homme
Keyword(s):  
High Dose ◽  
General Anesthetics ◽  
Visual Analogue Pain ◽  
Pain Scores ◽  
Arterial Blood ◽  
Sedation Scores ◽  
Group 3 ◽  
Group 2 ◽  
High Doses ◽  
Group 1

Background The rationale of this study was to compare high-dose epidural clonidine with a more commonly used agent, such as bupivacaine. This was performed to give a more objective idea of the relative analgesic potency of epidural clonidine. Methods Sixty patients undergoing intestinal surgery during propofol anesthesia were studied. At induction, the patients received epidurally a dose of 10 micrograms/kg [corrected] clonidine in 7 ml saline followed by an infusion of 6 micrograms [corrected] x kg(-1) x h(-1) (7 ml/h) (group 1, n = 20), a dose of 7 ml bupivacaine, 0.5%, followed by 7 ml/h bupivacaine, 0.25% (group 2, n = 20), or a dose of 7 ml bupivacaine, 0.25%, followed by 7 ml/h bupivacaine, 0.125% (group 3, n = 20). Intraoperatively, increases in arterial blood pressure or heart rate not responding to propofol (0.5 mg/kg) were treated with intravenous alfentanil (0.05 mg/kg). Additional doses of propofol were given to maintain an adequate bispectral index. The epidural infusions were maintained for 12 h. In cases of subjective visual analogue pain scores up to 5 cm at rest or up to 8 cm during coughing, the patients were given access to a patient-controlled analgesia device. Results During anesthesia, patients in group 1 required less propofol than those in groups 2 and 3 (78 [36-142] mg vs. 229 [184-252] mg and 362 [295-458] mg; P < 0.05) and less alfentanil than patients in group 3 (0 [0-0] mg vs. 11 [6-20] mg; P < 0.05). Analgesia lasted 380 min (range, 180-645 min) in group 1 versus 30 min (range, 25-40 min) in group 2 and 22 min (range, 12.5-42 min) in group 3 (P < 0.05). There was no suggestion of a hemodynamic difference among the three groups except for heart rates that were significantly reduced in patients in group 1. Sedation scores were significantly higher in this group during the first 2 h postoperatively. Conclusion Our results show that high doses of epidural clonidine potentiate general anesthetics and provide more efficient postoperative analgesia than the two bupivacaine dosage regimens investigated.

scholarly journals Quantifying prescribed high dose opioids in the community and risk of overdose

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Joe Schofield ◽  
Deborah Steven ◽  
Rebecca Foster ◽  
Catriona Matheson ◽  
Alexander Baldacchino ◽  
...  
Keyword(s):  
Risk Factors ◽  
Service Improvement ◽  
High Dose ◽  
Opioid Overdose ◽  
Opioid Prescribing ◽  
Patient Level ◽  
Strong Opioid ◽  
High Doses ◽  
Strong Opioids ◽  
Very High

Abstract Background Opioid prescribing for a range of health issues is increasing globally. The risk of fatal and non-fatal overdose is increased among people prescribed strong opioids: in high doses in the context of polypharmacy (the use of multiple medications at the same time), especially with other sedatives; and among people with multiple morbidities including cardiorespiratory, hepatic and renal conditions. This study described and quantified the prescribing of strong opioids, comorbidities and other overdose risk factors among those prescribed strong opioids, and factors associated with high/very high opioid dosage in a regional health authority in Scotland as part of a wider service improvement exercise. Methods Participating practices ran searches to identify patients prescribed strong opioids and their characteristics, polypharmacy, and other overdose risk factors. Data were anonymised before being analysed at practice and patient-level. Morphine Equivalent Doses were calculated for patients based on drug/dose information and classed as Low/Medium/High/Very High. Descriptive statistics were generated on the strong opioid patient population and overdose risk factors. The relationship between the prescribing of strong opioids and practice/patient-level factors was investigated using linear and logistic regression models. Results Eighty-five percent (46/54) of GP practices participated. 12.4% (42,382/341,240) of individuals in participating practices were prescribed opioids and, of these, one third (14,079/42,382) were prescribed strong opioids. The most common comorbidities and overdose risk factors among strong opioid recipients were pain (67.2%), cardiovascular disease (43.2%), and mental health problems (39.3%). There was a positive significant relationship between level of social deprivation among practice caseload and level of strong opioid prescribing (p < 0.001). People prescribed strong opioids tended to be older (mean 59.7 years) and female (8638, 61.4%) and, among a subset of patients, age, gender and opioid drug class were significantly associated with prescribing of High/Very High doses. Conclusions Our findings have identified a large population at potential risk of prescription opioid overdose. There is a need to explore pragmatic models of tailored interventions which may reduce the risk of overdose within this group and clinical practice may need to be tightened to minimise overdose risk for individuals prescribed high dose opioids.

Immunomodulating functions of recombinant ovine interferon tau: potential for therapy in mulitple sclerosis and autoimmune disorders

1998 ◽  
Vol 4 (2) ◽  
pp. 63-69 ◽  
Author(s):  
O A Khan ◽  
H Jiang ◽  
P S Subramaniam ◽  
H M Johnson ◽  
S S Dhib-Jalbut
Keyword(s):  
Therapeutic Option ◽  
High Dose ◽  
Interferon Tau ◽  
Unique Type ◽  
Immune Mediated ◽  
High Concentrations ◽  
Ifn Treatment ◽  
High Doses ◽  
Mulitple Sclerosis

The interferons (IFN) are a family of complex proteins possessing antiviral, antiproliferative, and immunomodulatory activities. Two type 1 recombinant human IFN have been recently approved for the treatment of multiple sclerosis (MS). However, use of high dose type 1 IFN treatment in MS patients has been limited by dose-related toxicity. Ovine IFNt is a unique type 1 interferon discovered for its role in the animal reproductive cycle. It differs from other type 1 IFNs in that it is remarkably less toxic even at high concentrations, is able to cross species barriers, and is not inducible by viral infection. Ovine IFNt has been shown to be very effective in the treatment of animal models of MS. In this study, we examined the toxicity of OvIFNt on human T-cells at high doses and its immunregulatory properties at equivalent doses. Our experiments confirmed the remarkably non-toxic nature of OvIFNt on human cells at high concentrations as well as immunomodulating properties consistent with other type 1 IFNs including an antilymphoproliferative effect and inhibition of IFNg-induced HLA class II expression. These results suggest that OvIFNt could be developed into a potentially less toxic therapeutic option for immune-mediated disorders including MS.

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