scholarly journals Controlled deterioration test to evaluate the physiological potential of sweet corn seeds

2021 ◽  
Vol 42 (3) ◽  
pp. 949-962
Author(s):  
Jéssica de Lucena Marinho ◽  
◽  
José Henrique Bizzarri Bazzo ◽  
Claudemir Zucareli ◽  
Inês Cristina de Batista Fonseca ◽  
...  

The evaluation of the physiological potential of sweet corn seeds is essential in a quality control program and the choice of vigor tests using an adequate analysis methodology is essential to obtain reliable results. In this sense, this study aimed to standardize the methodology of the controlled deterioration test through combinations between time and temperature to evaluate the vigor of sweet corn seeds. Six lots of sweet corn seeds were characterized by the degree of moisture and the initial physiological potential through the following tests: germination, first germination count, cold test, electrical conductivity, accelerated aging, length and dry mass of normal seedlings, seedling emergence in sand, and emergence rate index. Subsequently, these lots had their initial water content adjusted to 20% using the wet substrate method. The seeds from the six lots were subjected to the controlled deterioration test under two temperatures for four periods, obtaining a completely randomized design in an 8 × 6 factorial scheme with eight combinations of time/temperature and six lots. Germination and the first germination count were evaluated after the deterioration test. The data were subjected to analysis of variance with a comparison of means by the Tukey test at a 5% significance. The results of the first count and germination tests after deterioration were subjected to a simple linear correlation study together with the initial characterization data. The combinations 8 h – 40 °C and 8 h – 45 °C in the controlled deterioration test were efficient in distinguishing the vigor of seed lots. The shorter exposure period and the first germination count after deterioration are more suitable for evaluating the vigor of sweet corn seeds.

2017 ◽  
Vol 38 (6) ◽  
pp. 3491
Author(s):  
Paulo Alexandre Fernandes Rodrigues de Melo ◽  
Tatiane Sanches Jeromini ◽  
Carlos Eduardo Affonso ◽  
Edna Ursulino Alves ◽  
Cibele Chalita Martins

The correct assessment of the physiological quality of seed lots is necessary for the quality control program of companies. For such purpose, tests that detect differences in the physiological potential of seed lots and that meet the minimum market requirements. Thus, the study was conducted towards assessing the efficiency of laboratory tests in differentiating the quality of Brachiaria brizantha cv. Xaraés seed lots. Seeds from nine lots were assessed regarding water content, germination, first germination count, electrical conductivity and seedling emergence in sand in the laboratory (normal seedlings, first count and germination rate index), and the results were compared with those from the seedling emergence test conducted in the field. The experimental design used was completely randomized, with four replicates, and the Pearson correlation coefficient between the values from the germination, vigor and field seedling emergence tests was determined. The germination and seedling emergence in sand tests and the seedling emergence rate index efficiently assess the physiological quality of Brachiaria brizantha cv. Xaraés seed lots, providing data similar to those from seedling emergence in the field.


Author(s):  
Paulo Bruno Santos das Virgens ◽  
Taise Almeida Conceição ◽  
Rafael Marani Barbosa

Evaluating the vigour and viability of seeds can help in the production of seed lots with proven high physiological potential. In this regard, the tetrazolium (TZ) test is efficient, rapid and inexpensive; however, it not been fully explored for forest species. Further development of species-specific methodologies is required in order to maximise the test efficacy. The objective of this work was to define a tetrazolium test protocol to evaluate the vigour and viability of Genipa americana L seeds. Fruits and seeds were characterised for biometry and initial water content before and after hydration. Germination, germination speed index, electrical conductivity and seedling emergence was also performed. For the TZ test, G. americana seeds were cut longitudinally and submitted to different seed pre-conditioning methods (with and without 24 hours imbibition), different TZ concentrations (0.05, 0.075, 0.10 and 0.25%) and three immersion periods (2, 3 and 4 hours). The tetrazolium test allowed adequate evaluation of seed vigour levels (Viable; Viable and non-vigourous; and Non-viable). Immersion of non-imbibed seeds in 0.10% TZ solution, for three hours is the most efficient protocol for evaluating the vigour and viability of G. americana seeds.


Biologia ◽  
2017 ◽  
Vol 72 (8) ◽  
Author(s):  
M. Teresa Mas ◽  
Antoni M.C. Verdú ◽  
Marta Ginovart ◽  
Ramon Josa

AbstractIt is well known that soil sealing strongly affects seedling emergence. The effect of soil sealing on the emergence of flax and turnip seedlings was studied in the laboratory. Seeds were sown in pots, watered, then covered with loamy soil and water was added. Three different doses of water were tested. Soil sealing was obtained with a paste of soil mixed with distilled water, added to each pot as a thick homogeneous, continuous and isotropic layer. The initial water content of the seal was measured. When seedling emergence was observed (or at the end of the experiment in the case of event failure), seal strength was measured in situ by a firmness pressure tester (used as a penetrometer). Relationships between water loss and initial moisture of the seal versus mechanical impedance were obtained. Differences in emergence success between species depended on the initial soil water content as well as on the initial seal moisture. A model of seedling emergence success of the two species, flax and turnip, as a function of the initial seal moisture content was obtained using a binary logistic regression model.


2013 ◽  
Vol 35 (3) ◽  
pp. 340-346 ◽  
Author(s):  
Renata Oliveira Alvarenga ◽  
Julio Marcos-Filho ◽  
Tathiana Silva Timóteo

The assessment of physiological potential is essential in seed quality control programs. This study compared the sensitivity of different procedures for evaluating super sweet corn seed vigor, focusing on the primary root protrusion test. Six seed lots, each of the SWB 551 and SWB 585 hybrids, were used. Seed physiological potential was evaluated by germination and vigor tests (speed of germination, traditional and saturated salt accelerated aging, cold test, seedling length, seedling emergence and primary root protrusion). Primary root protrusion was evaluated every 12 hours at 15 °C, 20 °C and 25 °C using two criteria (primary root protrusion and seedlings at the 2 mm root stage). It was concluded that the primary root protrusion test at 15 °C can evaluate super sweet corn seed vigor by counting the number of seedlings at the 2 mm root stage.


Author(s):  
Jung Keun Choi ◽  
Mi A Son ◽  
Hyun Kyung Kim ◽  
Domyung Paek ◽  
Byung Soon Choi

Author(s):  
Xiaobing Li ◽  
Jianpeng Chen ◽  
Xiuqing Hu ◽  
Hongtao Fu ◽  
Jun Wang ◽  
...  

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 90
Author(s):  
Andrzej Bryś ◽  
Joanna Bryś ◽  
Marko Obranović ◽  
Dubravka Škevin ◽  
Szymon Głowacki ◽  
...  

The olive oil industry represents an important productive sector in the Mediterranean basin countries. Olive stone is an essential by-product generated in the olive oil extraction industries and it represents roughly 10% by weight of the olive fruit. The seeds of pickled olives are also a significant waste product. In the present study, we have investigated the possibility of the use of differential scanning calorimetry for the thermal characterization of seeds from green and black pickled olives from Croatia. The differential scanning calorimeter (DSC) with a normal pressure cell equipped with a cooling system was used to determine the thermal properties of seeds from olives. The following analyses were also performed: the determination of calorific values in a pressure bomb calorimeter, the determination of initial water content, the determination of changes of water content during drying at the temperatures of 30 °C, 50 °C and 80 °C, the determination of a percentage content of seeds mass to the mass of the whole olives, and the determination of ash content. Seeds from olives are characterized by very good parameters as a biomass. The analyzed olive seeds were characterized by low water content, low ash content, and a relatively high caloric value.


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