A consideration on criteria of blood cell counts for radiation workers. Variation of white blood cells within each individual.

Objectives: To determine the effects of Allium sativum essential oil (ASEO)phytotherapy on serum triglycerides, total cholesterol, HDLc, LDLc and blood cell counts inalbino rat model. Study design: Experimental study. Setting and Duration: Animal House,Sindh Agriculture University and Isra University Hyderabad from May 2014 to January 2015.Materials and Methods: 60 albino rats were divided into four groups. Controls were givenPlacebo. Experimental rat groups were given ASEO 100 mg/kg, 200 mg/kg and 300 mg/kgorally for 30 days. Cardiac puncture was performed for blood sampling. Research variableswere analyzed on Statistix 10.0 (USA). Results: Blood lipids showed significant reduction invarious blood lipid fractions. Serum LDLc exhibited with a concomitant rise in serum HDLc (p=0.0001) in high ASEO treated rats. Red blood cells, white blood cells and platelet showedsignificant improvement ASEO fed rats (p=0.001). Conclusion: Allium sativum essential oil(ASEO) phytotherapy showed a rise in HDLc and a reduction in LDLc, triglycerides and totalcholesterol with improvement in red blood cell counts.

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OVALOCYTOSIS ASSOCIATED WITH THE SICKLE CELL TRAIT

Blood ◽

10.1182/blood.v4.5.505.505 ◽

1949 ◽

Vol 4 (5) ◽

pp. 505-510 ◽

Cited By ~ 6

Author(s):

ROBERT S. FADEM

Keyword(s):

Blood Cell ◽

Red Blood Cells ◽

Blood Cells ◽

Sickle Cell Trait ◽

Daily Variation ◽

Blood Indices ◽

Sickle Cells ◽

Cell Counts ◽

Blood Cell Counts ◽

Saline Solutions

Abstract 1. A patient has been presented whose circulating red blood cells were composed of 65-84 per cent ovalocytes, 3-11 per cent sickled cells, and some normal appearing discoid cells. 2. The red blood cell counts and the blood indices were within normal limitations. 3. The red blood cells showed an increased resistance to hypotonic saline solutions. See PDF for Table See PDF for Table See PDF for Table 4. The peripheral blood showed a daily variation in the percentage of circulating ovalocytes, from 65 per cent to 84 per cent, and in the percentage of circulating sickle cells, from 3 per cent to 11 per cent. 5. After 72 hours in fresh wet preparations the per cent of ovalocytes remained essentially unchanged from that of fresh fixed blood. 6. The percentage of sickled cells was found to be increased after 18, 24, and 72 hours in fresh wet preparations as compared to the percentage of sickled cells found in fresh fixed preparations. 7. Some of the normal appearing discoid red blood cells were observed to sickle in fresh wet preparations within 18 hours.

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Accuracy of routine biomarkers and blood leucocytes count to assist diagnosis of COVID-19-associated pneumonia in adult patients visiting the emergency department

10.21203/rs.3.rs-34817/v1 ◽

2020 ◽

Author(s):

Cécile Maincent ◽

Frédéric Berthier ◽

Ryan Lukas Farhad ◽

Cristel Fissore-Magdelein ◽

Claire Claire Dittlot ◽

...

Keyword(s):

Emergency Department ◽

Blood Cell ◽

White Blood Cells ◽

Adult Patients ◽

Predictive Values ◽

Cell Counts ◽

Blood Cell Counts ◽

Low Probability ◽

White Blood Cell Counts ◽

Adjudication Committee

Abstract Background To investigate whether routine biomarkers and blood leucocytes count could assist diagnosis of COVID-19-associated pneumonia in adult patients visiting the emergency department (ED). Methods This monocentre retrospective study enrolled 254 patients with nasopharyngeal RT-PCR for SARS-COV-2, routine biomarkers (D-dimers, fibrinogen, C-reactive protein, procalcitonin, NTpro-BNP, cTnT-hs) and blood cell counts. Sensitivity and specificity were evaluated. An adjudication committee classified diagnostic probability as certain, probable, unlikely, and excluded, based on all available data, then distributed in 2 categories: high (certain and probable) and low probability (unlikely and excluded). Results Between 25 th of February and 15 th of April, 2020, 254 of 388 patients could be analyzed. The adjudication committee classified 46 patients as definite, 18 as probable, 64 as unlikely, and 126 as excluded, corresponding to 64 high and 190 low probability. High and low probability patients differed for fibrinogen (P<0.0005) and white blood cell counts, notably leucocytes (P=0.0015), neutrophilic (P=0.0036), lymphocytes (P=0.0057), eosinophilic (P=0.027), and basophilic (P<0.001) counts. In a multivariate analysis, basophilic count < 25/µL (OR 3.048 [95%CI; 1.34-6.919]), neutrophilic count < 4000 /µL (OR 5.525 [95%CI; 2.20-13.855], and fibrinogen > 3g/L (OR 6355 [95%CI; 2.01-20.079] were independently associated with the diagnosis. Negative predictive values were 0.98 and 0.93 combining fibrinogen ( < 3g/L) and eosinophilic count ( < 80/µL), and fibrinogen and basophilic count ( < 25/µL), respectively. Conclusion Changes in fibrinogen and white blood cells, notably basophilic count, showed interesting performance for the diagnosis COVID-19 associated pneumonia. Combining fibrinogen with either eosinophilic or basophilic count was helpful to exclude the diagnosis.

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Moderate Range Static Magnetic Field Promoted Variation of Blood Parameters: An In vitro Study

ARO-The Scientific Journal of Koya University ◽

10.14500/aro.10612 ◽

2020 ◽

Vol 8 (1) ◽

pp. 55-64

Author(s):

Bestoon T. Mustafa ◽

Sardar P. Yaba ◽

Asaad H. Ismail

Keyword(s):

Magnetic Field ◽

Blood Cell ◽

Static Magnetic Field ◽

Blood Cells ◽

White Blood Cells ◽

Albino Rats ◽

Field Exposure ◽

Blood Samples ◽

Cell Counts

This study was undertaken to investigate the influence of a homogenous and uniform static magnetic field (SMF) on the main blood cell counts in vitro experiment. Fresh blood samples were collected from albino rats and exposed to SMF (2.4, 6, 25, 50, 75, and 100 mT) versus 15–60 min. Results showed a significant change of blood counts under the low field effects. A 2.4 mT was a trend of white blood cells (WBCs) count increase non-linearly. However, a 6 mT exposure reduced WBCs with about 39%. Other variations fluctuated within 30%. The 25 mT decreased red blood cells (RBCs), hemoglobin, and hematocrit levels with 13% similarly. The lower exposure field, (2.4 and 6) mT, and effects on RBCs were 6% fluctuation. The 6 mT reduced platelet counts with half in comparison to control blood samples. About 20% increase obtained due to 50 mT exposure during all period. None of 75 and 100 mT exposures dominated blood counts alterations. The quiet magnetic field exposure for a certain time can be interesting to control blood cell count-related diseases.

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Determination of an optimal dilution of virulent feline infectious enteritis (panleucopaenia) virus for challenge purposes

Epidemiology and Infection ◽

10.1017/s0022172400042479 ◽

1970 ◽

Vol 68 (4) ◽

pp. 549-556 ◽

Cited By ~ 8

Author(s):

K. J. O'Reilly

Keyword(s):

Influenza Virus ◽

Blood Cells ◽

White Blood Cells ◽

Geometric Means ◽

Virus Challenge ◽

Cell Counts ◽

Blood Cell Counts ◽

Virulent Virus ◽

White Blood Cell Counts

SUMMARYWhen ten cats were infected orally with undiluted or a 10−1 dilution of virulent feline infectious enteritis (panleucopaenia) virus, all developed severe leucopaenia followed by the development of demonstrable antibody, but none died. Eighteen of 29 cats given a 10−2 dilution of virus died of the disease. Three of the survivors had white blood cell counts of less than 4000 and three had counts between 4000 and 6000 cells. Although the remaining five animals never had individual counts of less than 6000 cells, the geometric means of these counts showed that a marked depression in the leucocyte counts had occurred. All surviving cats developed antibody.Among the ten cats dosed with either 10−3 or 10−4 dilution of virus, four died of feline infectious enteritis and three developed antibody after falls in the leucocyte counts. It is suspected that low dilutions of feline infectious enteritis virulent virus in cats produce a phenomenon similar to that reported by von Magnus (1954) with influenza virus in eggs.Leucopaenia is commonly defined as less than 4000 white blood cells/mm.3 of blood. Counts lower than this are usual in cats which either die of the disease or have received large doses of virus; they are less common in cats surviving after administration of diluted virus. Challenge of cats with pre-existing antibody did not provoke a depression in the leucocyte counts.

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PSIV-27 Peripheral complete blood cell count results in pigs are impacted by gender, sow parity, and farrowing season

Journal of Animal Science ◽

10.1093/jas/skaa278.525 ◽

2020 ◽

Vol 98 (Supplement_4) ◽

pp. 291-292

Author(s):

Elle Rottman ◽

Alisun N Watson ◽

Catherine Buck ◽

Tsungcheng Tsai ◽

Jeffery J Chewning ◽

...

Keyword(s):

Blood Cell ◽

Cell Count ◽

Whole Blood ◽

Blood Cells ◽

White Blood Cells ◽

Mean Corpuscular Hemoglobin ◽

Complete Blood Cell Count ◽

Blood Cell Count ◽

Corpuscular Hemoglobin ◽

Cell Counts

Abstract Complete blood cell counts have been used as a diagnostic tool across many animal species including swine. To investigate the factors that cause variation in complete blood cell count results, a total of 2,284 whole blood samples were collected from 2012 to 2019 in preweaning piglets (n = 518), nursery pigs (n = 1,704), and grower pigs (n = 60). Whole blood was collected into K2EDTA blood collection tubes and assayed using an automatic hematologic analyzer within 6 hours of collection. Data were analyzed by Mixed procedure of SAS with gender, parity group, and farrowing season as fixed effects. Body weight and age of pigs served as covariances. Farrowing season was grouped into summer (born during May to October) or winter (or November to April). Pigs that were born from first, second, and third parity, and four and above parity sows were assorted into parity group 1, 2 to 3, and 4+, respectively. Barrows had a greater concentration of total white blood cells (P < 0.01), lymphocytes (P < 0.01), and neutrophils (P < 0.01) compared to gilts. Barrows had lower mean corpuscular volume (P = 0.03), mean corpuscular hemoglobin (P < 0.01), and mean corpuscular hemoglobin concentration (P = 0.02) compared to gilts. Pigs that were farrowed in the winter season had a greater concentration of white blood cells (P = 0.01), neutrophils (P = 0.01), and the percentage of neutrophils (P = 0.03), but were lower in the percentage of lymphocytes (P = 0.03) compared to pigs farrowed during summer. Pigs born to parity four and above sows obtained a greater lymphocyte count (P = 0.01), percentage of neutrophils (P = 0.02), and percentage of lymphocytes (P = 0.01). We concluded that peripheral complete blood cells count results were affected by gender, farrowing season, and sow parity.

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High intake of vegetables is linked to lower white blood cell profile and the effect is mediated by the gut microbiome

BMC Medicine ◽

10.1186/s12916-021-01913-w ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Cristina Menni ◽

Panayiotis Louca ◽

Sarah E. Berry ◽

Amrita Vijay ◽

Stuart Astbury ◽

...

Keyword(s):

Blood Cell ◽

Chronic Inflammation ◽

White Blood Cell ◽

Gut Microbiome ◽

Blood Cells ◽

Vegetable Intake ◽

White Blood Cells ◽

Cell Counts ◽

Habitual Diet ◽

Cell Profile

Abstract Background Chronic inflammation, which can be modulated by diet, is linked to high white blood cell counts and correlates with higher cardiometabolic risk and risk of more severe infections, as in the case of COVID-19. Methods Here, we assessed the association between white blood cell profile (lymphocytes, basophils, eosinophils, neutrophils, monocytes and total white blood cells) as markers of chronic inflammation, habitual diet and gut microbiome composition (determined by sequencing of the 16S RNA) in 986 healthy individuals from the PREDICT-1 nutritional intervention study. We then investigated whether the gut microbiome mediates part of the benefits of vegetable intake on lymphocyte counts. Results Higher levels of white blood cells, lymphocytes and basophils were all significantly correlated with lower habitual intake of vegetables, with vegetable intake explaining between 3.59 and 6.58% of variation in white blood cells after adjusting for covariates and multiple testing using false discovery rate (q < 0.1). No such association was seen with fruit intake. A mediation analysis found that 20.00% of the effect of vegetable intake on lymphocyte counts was mediated by one bacterial genus, Collinsella, known to increase with the intake of processed foods and previously associated with fatty liver disease. We further correlated white blood cells to other inflammatory markers including IL6 and GlycA, fasting and post-prandial glucose levels and found a significant relationship between inflammation and diet. Conclusion A habitual diet high in vegetables, but not fruits, is linked to a lower inflammatory profile for white blood cells, and a fifth of the effect is mediated by the genus Collinsella. Trial registration The ClinicalTrials.gov registration identifier is NCT03479866.

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The New Hematology Analyzer Sysmex XE-2100

Archives of Pathology & Laboratory Medicine ◽

10.5858/2001-125-0391-tnhasx ◽

2001 ◽

Vol 125 (3) ◽

pp. 391-396 ◽

Cited By ~ 5

Author(s):

Katharina Ruzicka ◽

Mario Veitl ◽

Renate Thalhammer-Scherrer ◽

Ilse Schwarzinger

Keyword(s):

Blood Cell ◽

White Blood Cell ◽

Blood Cells ◽

White Blood Cells ◽

Reference Method ◽

University Hospital ◽

National Committee ◽

Medical Electronics ◽

Cell Counts ◽

Hematology Analyzer

Abstract Context.—The new hematology analyzer Sysmex XE-2100 (TOA Medical Electronics, Kobe, Japan) has a novel, combined, white blood cell differential technology and a special reagent system to enumerate nucleated red blood cells. Design.—Performance evaluation of both technologies of the Sysmex XE-2100 according to the H20-A protocol of the National Committee for Clinical and Laboratory Standards and comparison of the results with those for the hematology analyzer Sysmex NE-8000 (TOA Medical Electronics). Specimens.—Five hundred forty-four blood samples randomly chosen from various inpatient and outpatient departments of the Vienna University hospital. Results.—Five-part white blood cell differential counts on the XE-2100 revealed excellent correlation with the manual reference method for neutrophils, lymphocytes, and eosinophils (r = .925, .922, and .877, respectively) and good correlation for monocytes and basophils (r = .756 and .763, respectively). The efficiency rates of flagging for the presence of ≥1% abnormal white blood cells were 83% (XE-2100) and 66% (NE-8000). The correlation of automated and microscopic nucleated red blood cell counts was excellent (r = .97). Conclusions.—From the present evaluation and our former experience with other types of Sysmex analyzers, we conclude that the new white blood cell differential technology of the XE-2100 represents a further development toward more efficient flagging of abnormal white blood cells.

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Imatinib mesylate has an inhibitory effect on hematopoiesis in a mice model

Blood ◽

10.1182/blood.v122.21.5568.5568 ◽

2013 ◽

Vol 122 (21) ◽

pp. 5568-5568

Author(s):

Jie yu Ye ◽

Su yi Li ◽

En yu Liang ◽

Fan yi Meng ◽

Beng Chong ◽

...

Keyword(s):

Bone Marrow ◽

Blood Cell ◽

Tyrosine Kinase ◽

Imatinib Mesylate ◽

Blood Cells ◽

White Blood Cells ◽

Mice Model ◽

Cell Counts ◽

Number Of Patients ◽

And Control

Abstract Imatinib mesylate (Gleevec or STI-571), a tyrosine kinase inhibitor, can potently block both platelet-derived growth factor (PDGF) receptor-α and β by inhibiting the receptor tyrosine kinase activity. Imatinib mesylate has been successfully used to treat patients with chronic myeloid leukemia (CML) and Ph positive acute lymphoid leukemia (ALL). However, a significant number of patients with imatinib mesylate treatment have developed neutropenia, thrombocytopenia and anemia (van Deventer, Hall et al. 2002). Our previous studies have demonstrated that PDGF/PDGFR plays an important role on thrombopoiesis, and blockage of PDGFR by imatinib inhibited the downstream signal pathway, which led to reduce of megakaryocyte production (Ye, et al. Haematologica, 2010). This may partially explain the imatinib-related thrombocytopenia. However, the role of imatinib on normal hematopoiesis remains to be investigated. In the present study, we identified the expression of PDGFR-β on the human megakaryoblastic cells: Meg-01, CHRF-288-11, Dami and M-07e, human erythromyeloblastoid leukemia cells: K-562 and human myeloblastic cells: HL-60 by RT-PCR, western blot and flow cytometry. We then demonstrated the effect of imatinib on normal hematopoiesis in the 8 wk old C57BL/6 mice model. Gleevec (25 mg/kg/day) dissolved in distilled water or water only were given orally for up to 2 wks. 15ul whole blood was taken from postorbital weekly to perform blood cell counts. The Gleevec -treated group showed a reduction of platelet count as compared to the control at day 14 (543.3±71.95×109/L vs. 330.0±62.23×109/L, n=5, P<0.05). Similarly, Gleevec caused a significant decrease in the number of white blood cells on day 14 (9.667×±0.8511×109/L vs. 6.080±0.8015×109/L, n=5, P<0.05). While no significant differences were found on red blood cell count between Gleevec and control groups. The mice bone marrow was harvested from each group to perform CFU assays. Gleevec significantly inhibited the formation of bone marrow CFU-MK (15.33±1.764 vs 3.333±0.6667, n=3), and CFU-GM(13.50±2.784 vs 6.333±0.7265, n=3) compared with the control. There were no significant differences found in CFU-GEMM and CFU/BFU-E formation between the Gleevec and control treatment. Our studies suggested that PDGFR were expressed on hematopoietic cells. Imatinib mesylate, an inhibitor of PDGFR, not only played an important role in malignant hematopoiesis, but also in normal hematopoiesis. Administration of Gleevec reduced white blood cells and megakaryocytes/platelets production, which implied its potential therapeutic role in treating myeloproliferative diseases, such as Essential Thrombocytosis. Disclosures: No relevant conflicts of interest to declare.

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Evaluating Accuracy of DNA Pool Construction Based on White Blood Cell Counts

Frontiers in Genetics ◽

10.3389/fgene.2021.635846 ◽

2021 ◽

Vol 12 ◽

Author(s):

Amy N. Abrams ◽

Tara G. McDaneld ◽

John W. Keele ◽

Carol G. Chitko-McKown ◽

Larry A. Kuehn ◽

...

Keyword(s):

Blood Cell ◽

Dna Extraction ◽

White Blood Cell ◽

Univariate Analysis ◽

White Blood Cells ◽

Individual Animal ◽

Blood Samples ◽

Cell Counts ◽

Blood Cell Counts ◽

White Blood Cell Counts

Pooling individual samples prior to DNA extraction can mitigate the cost of DNA extraction and genotyping; however, these methods need to accurately generate equal representation of individuals within pools. The objective of this study was to determine accuracy of pool construction of blood samples based on white blood cell counts compared to two common DNA quantification methods. Fifty individual bovine blood samples were collected, and then pooled with all individuals represented in each pool. Pools were constructed with the target of equal representation of each individual animal based on number of white blood cells, spectrophotometric readings, spectrofluorometric readings, and whole blood volume with 9 pools per method and a total of 36 pools. Pools and individual samples that comprised the pools were genotyped using a commercially available genotyping array. ASReml was used to estimate variance components for individual animal contribution to pools. The correlation between animal contributions between two pools was estimated using bivariate analysis with starting values set to the result of a univariate analysis. Adonis test on distance matrix from the animal correlation showed clustering with method, and higher correlations between methods than within (P < 1 × 10–6). White blood cell count was predictive of sample representation when compared to pooling based on DNA concentration. Therefore, constructing pools using white blood cell counts prior to DNA extraction may reduce cost associated with DNA extraction and genotyping and improve representation of individuals in a pool.

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