Improvement of the submergence stress tolerance of local South Sumatran rice through the introgression of the Sub1 gene by using marker-assisted selection

Submergence stress due to unpredictable soil flooding is one of the main constraints encountered in rainfed growing areas, especially in Southern Sumatran riparian swamps. The development of submergence-stress-tolerant cultivars through the introgression of Sub1 via marker-assisted backcrossing (MABC) is an ideal solution. This study was carried out during 2020 at Sriwijaya University, Palembang, Indonesia, with the aim to select Sub1-introgressed lines in BC3F1 generations on the basis of MABC and to screen out the SSR markers that were unlinked to the target gene for application in subsequent background selection studies. Results revealed that almost all the backcrossed progenies segregated from the rice parental cultivars ‘FR13A’ and ‘Pegagan’. The backcrossed lines showed significantly improved submergence stress tolerance and recovery rates compared with their parents. Sub1 introgression into the BC3F1 generation was confirmed by the tightly linked Sub1 marker SUB1C173, and marker RM23915 was used for recombinant selection. These markers followed the expected marker segregation ratio in accordance with the Mendelian single gene model. In the parental polymorphism survey, 84 out of 237 SSR markers that were unlinked to the target loci were found to be available for background study. Twenty-seven backcrossed lines were selected on the basis of foreground selection. Seven plants were selected on the basis of the recombinant marker RM23915. Five backcrossed lines were further selected on the basis of submergence stress tolerance and agronomic performance.

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Inheritance of Arabica Coffee Resistance to Radopholus similisCobb.

Pelita Perkebunan (a Coffee and Cocoa Research Journal) ◽

10.22302/iccri.jur.pelitaperkebunan.v23i1.53 ◽

2007 ◽

Vol 23 (1) ◽

Author(s):

Retno Hulupi ◽

Nasrullah . ◽

Soemartono .

Keyword(s):

Single Gene ◽

Gene Interaction ◽

Segregation Ratio ◽

Segregation Pattern ◽

Root Weight ◽

Resistant Parent ◽

Arabica Coffee ◽

Resistant Varieties ◽

Analysis System ◽

Almost All

A research to get inheritance of Arabica coffee resistance to Radopholus similisnematode was done in screen house and laboratory of Indonesian Coffee and Cocoa Research Institute, also at endemic area of coffee plantation, using F1, F1 R and F2 crossing between BP 542 A(resistant) x Andungsari 1 (susceptible) with their reciprocal, and BP 542 A x Kartika 1. The purpose of this study that was conducted at seedling stage is to formulate a Strategy for Arabica coffee breeding to get resistant varieties to nematode. As the variables of resistance were weight of seedling biomass, percent of root weight deviation, number of root nematodes, number of soil nematodes, reproduction and percent of necrotic root. Using discriminant analysis and fastclus, those data variables were analyzed for genetic of resistance with Statistical Analysis System programme version 8. Genetic study on the inheritance of resistance to R. similiswas started with evaluation of homozigosity of BP 542 A was resistant parent. The result showed that BP 542 A was heterozygous. Therefore, segregation test could not be suggested with segregation pattern principals as Mendel proposed. Segregation test on BP 542 A showed that it was heterozygote and the resistance was controlled by single gene with complete dominant effect, so the progeny segregated in 75% resistant and 25% susceptible. The result of the test showed the absence of maternal effect for root weight deviation and percentage of necrotic root variables, which meant that no cytoplasmic inheritance was involved. Based on the test of segregation ratio, almost all of the resistance was not appropriate for monogenic and or digenic segregation pattern as expected due to non allelic gene interaction that caused epistasis. Key words: Inheritance, resistance, Arabica coffee, Radopholus similis.

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Transmission Ratio Distortion Due to the bl Gene in Table Beet

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.126.3.340 ◽

2001 ◽

Vol 126 (3) ◽

pp. 340-343 ◽

Cited By ~ 3

Author(s):

Diane Austin ◽

I.L. Goldman

Keyword(s):

Single Gene ◽

Recessive Gene ◽

Segregation Ratio ◽

Transmission Ratio Distortion ◽

Transmission Ratio ◽

Wild Type ◽

Gene Model ◽

Male Gametes ◽

Ratio Distortion ◽

The Individual

The bl gene conditions a blotchy phenotype (irregular sectors of red and white root color) in table beet (Beta vulgaris ssp. vulgaris). Segregation of the bl gene was found to be consistent with a single recessive gene, however, some evidence for a departure from a single gene model was observed when blbl plants were used as females. In this report, segregation of the bl gene was examined in greater detail in 10 F2 populations derived from crosses of red blotchy-rooted females (genotype blbl, denoted blotchy) with red-rooted males (BlBl, denoted red,), and 10 F2 populations derived from the reciprocal cross. In blbl × BlBl crosses, the proportion of red-rooted progeny was greater than 0.75 in seven of the crosses, and was significantly greater (P = 0.005) in three crosses. A test for heterogeneity was significant, indicating that the proportion of red-rooted progeny differed significantly in these 10 crosses. In BlBl × blbl crosses, the proportion of red-rooted progeny was <0.75 in seven of the crosses and there were no significant departures from the expected 3:1 ratio in any of the individual crosses. However, a pooled estimate of the segregation ratio showed a significant (P < 0.01) departure from the 3:1 ratio (pooled estimate = 0.71). These data demonstrate transmission ratio distortion at the bl locus when blbl plants are used as both females and males in matings with wild type plants, but the degree of distortion is greater when blbl plants are used as females. Ratio distortion in such crosses may be due to a variety of factors, including increased transmission of the bl gene through female or male gametes depending on the direction of the cross, reduced fitness of maternally derived blbl progeny, epigenetic phenomena, increased fitness of paternally derived blbl progeny, or linkage of the bl gene to viability genes.

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A single gene all3940 (Dps) overexpression in Anabaena sp. PCC 7120 confers multiple abiotic stress tolerance via proteomic alterations

Functional & Integrative Genomics ◽

10.1007/s10142-015-0467-7 ◽

2015 ◽

Vol 16 (1) ◽

pp. 67-78 ◽

Cited By ~ 7

Author(s):

Om Prakash Narayan ◽

Nidhi Kumari ◽

Poonam Bhargava ◽

Hema Rajaram ◽

Lal Chand Rai

Keyword(s):

Abiotic Stress ◽

Stress Tolerance ◽

Abiotic Stress Tolerance ◽

Single Gene ◽

Anabaena Sp ◽

Pcc 7120

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CYSTIC FIBROSIS OF THE PANCREAS

PEDIATRICS ◽

10.1542/peds.19.6.1136 ◽

1957 ◽

Vol 19 (6) ◽

pp. 1136-1138

Author(s):

Paul A. di Sant'Agnese ◽

Charles Upton Lowe

Keyword(s):

Cystic Fibrosis ◽

Clinical Evidence ◽

Clinical Effect ◽

Single Gene ◽

Pancreatic Insufficiency ◽

Recessive Gene ◽

The United States ◽

Live Births ◽

Almost All ◽

Very High

IN THE COURSE of a review of all features of the disease, the following points were particularly noteworthy: Incidence This disease accounts for almost all cases of pancreatic insufficiency in children. The incidence in the population of the United States is between 1 in 600 and 1 in 10,000 live births, with a probable average incidence of 1 in 2,500. There is no sex predominance. There is, however, a difference in racial predilection, being rarely seen in the Negro and never in Mongolians. It is a familial disease, displaying the characteristics of a mendelian recessive gene. This means that in an affected family the disease may occur in approximately 25% of the offspring, that both parents must be carriers of the trait and that two-thirds of the non-affected children are also carriers. Birth order has no effect on the inheritance of this disease. The fact that it is usually a lethal disease indicates that the mutation rate for this gene must be very high; the frequency of the single gene in the population has been calculated to be approximately 1 in 50. Pancreatic Insufficiency Clinical evidence of poor digestion and absorption of protein and fat is seen in the increased quantities of these substances in the feces, which causes the feces to be bulky, foul smelling, foamy and greasy. Another clinical effect of malabsorption is seen in the failure of the newborn infant with cystic fibrosis of the pancreas to regain birth weight in the first 10 days of life. In the absence of other evidence of disease, this is a sign suggestive of pancreatic failure.

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Gene Transfer of Multiple Flowers and Pubescent Leaf from Capsicum chinense into Capsicum annuum Backgrounds

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.115.3.499 ◽

1990 ◽

Vol 115 (3) ◽

pp. 499-502 ◽

Cited By ~ 4

Author(s):

D.M. Shuh ◽

James F. Fontenot

Keyword(s):

Capsicum Annuum ◽

Female Parent ◽

Field Studies ◽

Segregation Ratio ◽

Gene Model ◽

Leaf Pubescence ◽

Segregation Ratios ◽

Selective Elimination ◽

Dominant Genes ◽

Made In

The inheritance of multiple flowers and leaf pubescence resulting from the crosses between accessions from pepper species Capsicum annuum L. and C. chinense Jacq. was examined. Hand cross- and self-pollinations were made in a glass greenhouse. Only eight normal F1 plants were obtained from crosses between the two species when C. annuum L. was the female parent. F2 and backcross generations obtained from the F, and the two parents were grown in the field. Two field studies indicated that multiple flowers and leaf pubescence were controlled by dominant genes. A three-gene model leading to an F2 segregation ratio of 45:9:10 and a two-gene model leading to an F2 segregation ratio of 13:3 were suggested for the inheritance of multiple flowers and leaf pubescence, respectively. Epistasis was evoked in the interpretation of the data. No linkage was found between the two characters. The inconsistencies between F2 and backcross data might be due to selective elimination of genes from one or the other parent in an Interspecific hybridization. Segregation ratios from intraspecific crosses for leaf pubescence supported a two-gene model and gave an F2 ratio of 13 pubescent leaf : 3 glabrous leaf progeny.

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Minimum Sample Size and Optimal Positioning of Flanking Markers in Marker-Assisted Backcrossing for Transfer of a Target Gene

Crop Science ◽

10.2135/cropsci1999.3961903x ◽

1999 ◽

Vol 39 (6) ◽

pp. 1903-1903

Author(s):

Matthias Frisch ◽

Martin Bohn ◽

Albrecht E. Melchinger

Keyword(s):

Sample Size ◽

Target Gene ◽

Minimum Sample Size ◽

Minimum Sample ◽

Marker Assisted Backcrossing

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Genetic diversity of cultivated and wild-type peanuts evaluated with M13-tailed SSR markers and sequencing

Genetics Research ◽

10.1017/s0016672307008695 ◽

2007 ◽

Vol 89 (2) ◽

pp. 93-106 ◽

Cited By ~ 37

Author(s):

NOELLE A. BARKLEY ◽

ROB E. DEAN ◽

ROY N. PITTMAN ◽

MING L. WANG ◽

CORLEY C. HOLBROOK ◽

...

Keyword(s):

Genetic Diversity ◽

Ssr Markers ◽

Polymorphic Information Content ◽

Data Set ◽

Mini Core Collection ◽

Genomic Ssr ◽

Cultivated Peanut ◽

The Mean ◽

Wild Accessions ◽

Almost All

SummaryThirty-one genomic SSR markers with a M13 tail attached were used to assess the genetic diversity of the peanut mini core collection. The M13-tailed method was effective in discriminating almost all the cultivated and wild accessions. A total of 477 alleles were detected with an average of 15·4 alleles per locus. The mean polymorphic information content (PIC) score was 0·687. The cultivated peanut (Arachis hypogaea L.) mini core produced a total of 312 alleles with an average of 10·1 alleles per locus. A neighbour-joining tree was constructed to determine the interspecific and intraspecific relationships in this data set. Almost all the peanut accessions in this data set classified into subspecies and botanical varieties such as subsp. hypogaea var. hypogaea, subsp. fastigiata var. fastigiata, and subsp. fastigiata var. vulgaris clustered with other accessions with the same classification, which lends further support to their current taxonomy. Alleles were sequenced from one of the SSR markers used in this study, which demonstrated that the repeat motif is conserved when transferring the marker across species borders. This study allowed the examination of the diversity and phylogenetic relationships in the peanut mini core which has not been previously reported.

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Inheritance of anthracnose resistance in the tropical pasture legume Stylosanthes hamata

Australian Journal of Agricultural Research ◽

10.1071/ar9951353 ◽

1995 ◽

Vol 46 (7) ◽

pp. 1353 ◽

Cited By ~ 3

Author(s):

N Iamsupasit ◽

DF Cameron ◽

M Cooper ◽

S Chakraborty ◽

LA Edye

Keyword(s):

Combining Ability ◽

Colletotrichum Gloeosporioides ◽

Single Gene ◽

General Combining Ability ◽

Tropical Pasture ◽

Gene Model ◽

Stylosanthes Hamata ◽

Moderately Resistant ◽

Half Diallel ◽

Pasture Legume

Eight tetraploid accessions of the tropical pasture legume Stylosanthes hamata with varying levels of response to the anthracnose pathogen (Colletotrichum gloeosporioides) were crossed in a half diallel scheme. Based on mean disease severity ratings (MDR), two parents, 55830 and 75164, were grouped as resistant (R), 55828 and 65365 were susceptible (S), and the remaining four, cvv. Verano and Amiga and 65371 and 75162 were moderately resistant (MR). Of these, the two resistant parents appear to carry different genes for resistance. The MDR of 20 of the 28 F2 populations was significantly different from their mid-parent MDR and the expression of resistance, in most cases, was recessive. Only a limited number of the F2 distributions for crosses between RxS, RxMR and MRxS parents conformed to a single gene model. The inheritance patterns observed were considered to be predominantly quantitative. Variation for general combining ability, was as large as or larger than that for specific combining ability suggesting that a large proportion of the genetic differences among the parents was additive. The finding that the resistance is inherited as a quantitative trait is consistent with results on the epidemiology of anthracnose in tetraploid S. hamata.

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Marker-assisted Backcrossing for Identification of Salt Tolerant Rice Lines

International Journal of Agricultural Research Innovation and Technology ◽

10.3329/ijarit.v2i2.14008 ◽

2013 ◽

Vol 2 (2) ◽

pp. 1-8 ◽

Cited By ~ 4

Author(s):

M Moniruzzaman ◽

MS Islam ◽

JA Rashid ◽

SN Begum ◽

MM Islam

Keyword(s):

Genetic Diversity ◽

Salt Tolerance ◽

Ssr Markers ◽

Marker Assisted Selection ◽

Genetic Maps ◽

Salt Tolerant ◽

High Yielding Variety ◽

Marker Assisted Backcrossing ◽

Tolerant Genotype ◽

New Cultivars

SSR or microsatellite markers are proved to be ideal for making genetic maps, assisting selection and studying genetic diversity in germplasm. SSR markers are playing important role to identify gene for salt tolerance that can be helpful for plant breeders to develop new cultivars. The experiment was conducted during the period from July 2009 to November 2010 in the experimental field and Biotechnology Laboratory of Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh to identify salt tolerant rice line of BC1F1 progenies of Binadhan-5 x FL-478 using SSR markers. Salt tolerant genotype, FL-478 was crossed with high yielding variety, Binadhan-5. Randomly selected 40 BC1F1 progenies along with their two parents (Binadhan-5, FL-478 and F1) were genotyped with microsatellite or SSR markers for identification of salt tolerant rice lines. Parental polymorphism survey was assayed by 10 SSR markers and three polymorphic SSR markers viz., RM 336, RM 510, and RM 585 were selected to evaluate BC1F1 rice lines for salt tolerance. In respect of Primer RM 336, 11 lines were found as salt tolerant and 25 lines were heterozygous and 3 lines were susceptible. Primer RM 510 identified two tolerant, 14 heterozygous and 22 susceptible lines. And primer RM 585 identified 4 lines as tolerant and 35 lines as susceptible. Thus, these markers could be efficiently used in tagging salt tolerant genes, in marker-assisted selection and quantitative trait loci (QTL) mapping. The selected BC1F1 could be used for developing BC2F1 and BC2F2 and mapping genes for salinity tolerance. DOI: http://dx.doi.org/10.3329/ijarit.v2i2.14008 Int. J. Agril. Res. Innov. & Tech. 2 (2): 1-8, December, 2012

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