scholarly journals Synergistic Effects between Mentha Piperita, Mentha Longifolia and Ocimum Basilicum on Different Bacterial Strains

2015 ◽  
Vol 7 (2) ◽  
pp. 170 ◽  
Author(s):  
Aveen Adham
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Bacterial Strain ◽  
Inhibitory Concentration ◽  
Synergistic Effects ◽  
Ocimum Basilicum ◽  
Mentha Piperita ◽  
Dilution Method ◽  
Bacterial Strains ◽  
Mentha Longifolia

The present study aimed to quantitatively evaluate antimicrobial activity of Mentha piperita, Mentha longifolia and Ocimum basilicum from Lamiaceae family, compare between them and to evaluate the type of interaction between them by microbroth dilution method and calculation of fractional inhibitory concentration. Dried leave of Mentha piperita, Mentha longifolia and Ocimum basilicum were extracted using same extraction method and solvent the highest ratio yielded by Mentha longifolia 9% and lowest ratio yielded by Ocimum basilicum 6%. Mentha piperita exhibit the lowest minimum inhibitory concentration value ranged between 1.5-<0.1 mg/ml, followed by Mentha longifolia and Ocimum basilicum ranged between 3-<0.1mg/ml with minimum bactericidal concentration ranged between 6-0.1875. On combination of leave extract Mentha piperita with Mentha longifolia exhibit the lowest minimum inhibitory concentration value ranged between 0.1875-<0.05 mg/ml, while combination of Mentha longifolia with Ocimum basilicum revealed minimum inhibitory concentration ranged between 0.75-<0.05. Among tested bacterial strain Streptococcus mitis showed high sensitivity against all tested leave extract alone and in combination form. The combinations of Mentha piperita, Mentha longifolia and Ocimum basilicum showed synergistic effects against most of bacteria. Mentha longifolia and Ocimum basilicum revealed antagonistic effect against Staphylococcus aureus. As a result three Lamiaceae species showed broad spectrum antimicrobial activity against isolated bacterial strain and their activity in combination form more pronounced than alone.

scholarly journals In vitro evaluation of the antimicrobial activity of Basil (Ocimum baslicum L.) and Coriander (Coriandrum satirum L.) oil extracts on Streptococus mutans

2018 ◽  
Vol 5 (2) ◽  
pp. 40 ◽  
Author(s):  
Mariana Lourenço ◽  
Adilson Cesar Abreu Bernardi ◽  
Nadia Lunardi ◽  
Rodolfo Jorge Boeck Neto ◽  
Patricia Sigilló Mazzoni Bernardi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Plant Extracts ◽  
Inhibitory Activity ◽  
Inhibitory Concentration ◽  
Growth Control ◽  
Ocimum Basilicum ◽  
Coriandrum Sativum ◽  
Microdilution Method

Plant extracts and essential oils show efficiency on growth control in a wide variation of microorganisms, including filamentous fungi, yeasts and bacteria. To evaluate antimicrobial activity of plant extracts, determine the lower quantity of substance to inhibit the microorganism test growth is necessary. This value is known as Minimum Inhibitory Concentration (MIC). This study had as aim to verify the antimicrobial action and the Minimum Inhibitory Concentration (MIC) of basil (Ocimum basilicum L.) and coriander (Coriandrum sativum L.) oil extract before S. mutans (ATCC 25175) strains. Antimicrobial activity determination was carried out by microdilution method and performed according to recommendations of CLSI (previously known as NCCLS), standard M7-A6 (NCCLS, 2003) for bacteria, and standard M27-A2 (NCCLS, 2002). All the experiments were carried out in triplicate. Results showed the Minimum Inhibitory Concentration (MIC) determination by microdilution method in broth showed Ocimum basilicum L. and Coriandrum sativum L. extract oils presented inhibitory activity before S. mutans strain. Basil in 1:4 concentration is bacteriostatic and in 1:3 concentration is bactericide. Coriander in 1:2 concentration is bacteriostatic and in 1:1 concentration is bactericide. We concluded that basil presented higher inhibitory activity regarding to the coriander. We also observed as bigger the extract dilution, lower their effectivity.o assess the attitude and practice of dental professionals towards using of advance Radiographic technique.

scholarly journals Evaluation of minimum inhibitory concentration and antimicrobial activity of basil extract (Ocimum basilicum L.) incorporated to the mouthwash

2014 ◽  
Vol 2 (5) ◽  
pp. 409
Author(s):  
Ana Carolina Marcantonio ◽  
Guilherme Oliveira ◽  
Eloisa Boeck ◽  
Thalita Pilon ◽  
Adilson Bernardi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Positive Control ◽  
Negative Control ◽  
Ocimum Basilicum ◽  
Verification Method ◽  
Microtiter Plates

AIM: This study has as aim to evaluate the minimum inhibitory concentration and the antimicrobial activity of basil extract incorporated to the mouthwash against the bacteria S.mutans. MATERIAL AND METHODS: For the study, the hydro alcoholic basil extract (Ocimum basilicum L.) incorporated to the mouthwash was used in order to evaluate the effect of this formulation on bacteria and its Minimum Inhibitory Concentration (MIC). As positive control, TBS + S. mutans was used; as negative control, only the TBS; the fluid hydro alcoholic basil extract 20% and the concentrated basil extract 12%, both incorporated to the mouthwash, were also evaluated. As MIC verification method and antimicrobial activity, the micro dilution was used in the concentrations: pure, 1/2, 1/4, 1/8, 1/16, 1/32, 1/64, 1/128; and carried out in triplicate. The microtiter plates were incubated and evaluated after 24 and 72hs. RESULTS: The results showed there was no antimicrobial activity of mouthwash associated to the fluid and concentrated basil extract. However, the mouthwash insulated showed antimicrobial activity only as pure; other dilutions did not presented the same result. CONCLUSION: Before the findings in this study, it is possible conclude that hydro alcoholic basil extract incorporated to the mouthwash did not present antimicrobial activity against the bacteria S. mutans.

scholarly journals Comparative evaluation of ginger extract and chlorhexidine on periodontal pathogens: an invitro study.

2019 ◽  
pp. 21-24
Author(s):  
Kesha Vaghani ◽  
Deepak Dave ◽  
Girish U Sailor
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Antimicrobial Property ◽  
Diffusion Method ◽  
Dilution Method ◽  
Periodontal Pathogens ◽  
Ginger Extract ◽  
Agar Disc ◽  
Mechanical Therapy

Brief Background: P. gingivalis, P. intermedia, T. forsythia and A. actinomycetemcomitans are considered to be the most commonly associated pathogens with periodontal disease. Chlorhexidine (CHX) is considered the gold standard for the chemical plaque control along with mechanical therapy. However, CHX has its own disadvantages. There has been a great swing from the drug of antibiotics to the use of remedial plant like ginger. Studies have shown that the existence of oxygenated mono- and sesquiterpenes, phenolic compounds (shogaol, gingerol) is responsible for the antimicrobial activity of ginger, that are lipid-soluble phenol compounds extracted from the ginger root Materials and Methods: The ginger extracts were prepared from fresh ginger. The different concentrations of extract - 1%, 5% and 10% - were used to determine the zone of inhibition by agar disc diffusion method against periodontal pathogens and compared to that of 0.2% chlorhexidine mouthwash. The minimum inhibitory concentration was also determined by serial dilution method. Results: Results of the study showed that the efficacy of all 3 ginger extracts showed comparatively less significant antimicrobial property against the microorganisms as compared to 0.2%chlorhexidine. The minimum inhibitory concentration was also found to be very high, i.e., 100μg/ml. Summary and Conclusions: There is no significant antimicrobial activity of ginger extract as compared to chlorhexidine Key Words: ginger extracts, chlorhexidine, periodontal pathogens, MIC

scholarly journals Antimicrobial Activity of the Dichloromethane Extract from in vitro Cultured Roots of Morinda royoc and Its Main Constituents

2010 ◽  
Vol 5 (5) ◽  
pp. 1934578X1000500
Author(s):  
Janetsy Borroto ◽  
Ricardo Salazar ◽  
Alejandro Pérez ◽  
Yemeys Quiros ◽  
Martha Hernandez ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Bacterial Strains ◽  
Yeast Strains ◽  
Mic Value

The dichloromethane extract and seven anthraquinones isolated from in vitro cultured roots of Morinda royoc L. were tested for their antimicrobial activity against seven yeast and seven bacterial strains. The extract showed a minimum inhibitory concentration (MIC) value of 15.6 μg/m against all species of Candida tested; except C. glabrata (MIC 1.95 μg/mL), and it inhibited the growth of oxacillin-resistant Staphylococcus aureus (MIC 31.2 μg/mL). Only morindone showed activity against all yeast strains (MIC 1.9 μg/mL), and against oxacillin-resistant Staphylococcus aureus (MIC 15 μg/mL).

scholarly journals Studies on meropenem and cefixime metal ion complexes for antibacterial activity

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Milind J. Umekar ◽  
Radheshyam T. Lohiya ◽  
Krishna R. Gupta ◽  
Nandkishore R. Kotagale ◽  
Neha S. Raut
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Metal Ion ◽  
Inhibitory Concentration ◽  
Resistant Bacteria ◽  
Dilution Method ◽  
Bacterial Strains ◽  
Uv Spectrum ◽  
Metal Ion Complexes ◽  
Form Complex

Abstract Background The metal ion complexes of meropenem and cefixime with cadmium, silver, palladium, zinc, nickel, cobalt and copper were synthesized and characterized by UV, FTIR and H1-NMR spectrophotometry. The antibacterial effects of the complexes were studied using cup and plate method against S. aureus, B. subtilis, E. coli, P. aeruginosa and K. pneumoniae for normal and resistant strains of bacteria. The minimum inhibitory concentration of the metal ion complexes was determined by broth dilution method. Results UV spectroscopic studies suggested that meropenem ligand form complex with different metal ions and FTIR spectrum confirmed the proposed structure. Similarly, UV spectrum of cefixime metal ion complexes at λmax 202–295 nm and meropenem metal ion complexes at λmax 249–304 nm was observed in all the complexes. FTIR peaks for a proposed structure were observed in all the meropenem and cefixime metal ion, indicating the formation of complexes, and retained the functional groups of drugs. Meropenem as well as cefixime metal ion complexes exhibited more antibacterial activity against all the selected bacterial strains. Specifically, the lowest minimum inhibitory concentration against P. aeruginosa and K. pneumoniae was observed to be 100 and 150 μg/ml, respectively. Conclusion The present study concluded that the meropenem and cefixime metal complexes can exhibit the better treatment than individual drug on normal as well as resistant bacteria.

scholarly journals Investigating the antimicrobial activity of extract prepared by ultrasound against Escherichia coli isolated from poultry stool

2020 ◽  
Vol 3 (2) ◽  
pp. 01-05
Author(s):  
Elham Javadian
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Withania Somnifera ◽  
Inhibitory Concentration ◽  
Rosmarinus Officinalis ◽  
Mentha Piperita ◽  
Antibiotic Resistant ◽  
Rotary Machine ◽  
Microdilution Method

Background: The aim of this study was to investigate the antimicrobial activity of Mentha piperita , Withania somnifera and Rosmarinus officinalis extract on antibiotic resistant Escherichia coli isolated from poultry stool in Zabol city. Materials & Methods: The extract of Mentha piperita, Rosmarinus officinalis and Withania somnifera was prepared by rotary machine. Escherichia coli strains were isolated from poultry stool samples. Minimum inhibitory concentration and minimum bactericidal concentration were determined by microdilution method. Results: The results of this study showed that Mentha piperita extract showed the highest inhibitory ratio compared to other extracts. The minimum inhibitory concentration was 3.1 ppm, with 5 strains inhibited at this concentration. Discussion: The results of this study could be an effective strategy for production and preparation of medicinal plants to help treating E. coli infections.

Green and Simple Synthesis of Silver Nanoparticles by Aqueous Extract of Perovskia abrotanoides: Characterization, Optimization and Antimicrobial Activity

2020 ◽  
Vol 21 (11) ◽  
pp. 1129-1137 ◽  
Author(s):  
Somayeh Mirsadeghi ◽  
Masoumeh F. Koudehi ◽  
Hamid R. Rajabi ◽  
Seied M. Pourmortazavi
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Minimum Inhibitory Concentration ◽  
Plant Extract ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Extraction Temperature ◽  
Silver Particles ◽  
Silver Nps ◽  
Perovskia Abrotanoides

Background: Herein, we report the biosynthesis procedure to prepare silver nanoparticles as reduction and capping agents with the aqueous plant extract of Perovskia abrotanoides. Methods: The therapeutic application of silver nanoparticles entirely depends on the size and shape of the nanoparticles therefore, their control during the synthesis procedure is so important. The effects of synthesis factors, for example, silver ion concentration, the mass of plant extract, reaction time and extraction temperature, on the size of silver particles were considered and optimized. Several analytical methods were used for the characterization of silver NPs including FT-IR and UV–Vis spectrophotometer, XRD and SEM. Results: The results showed that the mean size of the silver particles was about 51 nm. Moreover, the antibacterial properties of biosynthesized silver NPs were investigated by the minimum inhibitory concentration, minimum bactericidal concentration, and Well-diffusion tests. The minimum inhibitory concentration/ minimum bactericidal concentration values of silver NPs and aqueous plant extract versus Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and Gram-negative bacteria (E. coli) were 3.03/0.00, 1.20/0.01, 3.06/0.00, 0.98/1.04, 1.00/0.05 and 1.30/0.03 (mg/mL), respectively. Conclusion: The antimicrobial activity study displayed that the synthesized silver nanoparticles by plant extract have better antimicrobial properties compared to aqueous plant extract of Perovskia abrotanoides.

scholarly journals Antimicrobial Activity of Bauhinia Purpurea (L) by Minimum Inhibitory Concentration (MIC) Method

2017 ◽  
Vol 12 (01) ◽  
pp. 69-71
Author(s):  
Shamala T ◽  
A.M. Krupanidhi ◽  
Shanmukhappa S ◽  
B.E. Basavarajappa ◽  
Jayamma kulkarni
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Bauhinia Purpurea

scholarly journals Minimum inhibitory concentration of squid ink Loligo sp. extract on growth of Staphylococcus aureus and Streptococcus mutans bacteria

2020 ◽  
Vol 8 (3) ◽  
pp. 801
Author(s):  
Damajanty H. C. Pangemanan ◽  
Christy Mintjelungan
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Soft Tissues ◽  
Inhibitory Concentration ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Alternative Medicines ◽  
Pharmaceutical Properties

Background: Dental caries and periodontal disease can be caused by bacteria that attack hard and soft tissues in the oral cavity like Staphylococcus aureus and Streptococcus mutans bacteria One way to prevent infections caused by the bacteria are by using mouthwash. Currently, mouthwash that is widely used is mouthwash containing chlorhexidine, which has side effects in the form of tooth staining when used prolonged. The need for alternative medicines from nature that effectively cope with polymicrobial infections. One of the marine products that has pharmaceutical properties is squid, especially the ink it produces.Methods: This research is a pure experimental study (true experimental design) with a randomized pretest-posttest control group design. Squid ink Loligo sp. extract, obtained by extracting squid ink Loligo sp., with maceration method using 96% ethanol solvent. This research uses serial dilution method with spectrophotometric testing method.Results: After measuring the turbidity value in each treatment tube, it was found that at a concentration of 1.56% squid ink Loligo sp. extract began to inhibit the growth of Staphylococcus aureus and Streptococcus mutans bacteria.  This is proven because the absorbance value after and before incubation is fixed.Conclusions: The minimum inhibitory concentration of squid ink Loligo sp. extract on the growth of Staphylococcus aureus and Streptococcus mutants at a concentration of 1.56%.

scholarly journals Study and evaluation of antimicrobial activity and antioxidant capacity of dry extract and fractions of leaves of Raphanus sativus var. oleiferus Metzg.

2020 ◽  
Vol 36 (2) ◽  
Author(s):  
Ana Flávia da Silva ◽  
Marisa de Oliveira Lopes ◽  
Cláudio Daniel Cerdeira ◽  
Ingridy Simone Ribeiro ◽  
Isael Aparecido Rosa ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Minimum Inhibitory Concentration ◽  
Ethyl Acetate ◽  
Raphanus Sativus ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Fraction ◽  
Antimicrobial Activities ◽  
Dry Extract ◽  
Antioxidant And Antimicrobial Activities

The radish (Raphanus sativus L.) is a vegetable of the Brassicaceae family cultivated worldwide and has several medicinal properties. Its biological activities are related to various secondary metabolites present in the species, especially phenolics. Thus, the objectives of this study were the chemical analysis and evaluation of the antioxidant and antimicrobial activities of the dry extract and fractions of the fodder turnip leaves (R. sativus var. oleiferus Metzg.). Samples were analyzed by mass spectrometry and the antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical method and the reducing power method. Antimicrobial activity was determined by the agar diffusion and microdilution methods. The total phenols were concentrated in the butanol fraction (121.27 mg GAE/g) and the flavonoids were concentrated in the ethyl acetate fraction (98.02 mg EQ/g). The ethyl acetate fraction showed the best antioxidants results, with 83.45% of free radical scavenging and 11.34% of ferric ions reduction. The analysis of antimicrobial activity showed that the dry extract had the highest average zone of inhibition against Bacillus subtilis (18.67 mm). Smaller values of the minimum inhibitory concentration for Micrococcus luteus were, and the ethyl acetate fraction showed a lower minimum inhibitory concentration (0.1 mg/ml) for that microorganism. There was a strong correlation between the antioxidant activity and the content of phenols and flavonoids. The results showed the potential antioxidant and antimicrobial activities of this extract with the ethyl acetate fraction being most promising for further studies.

Sign in / Sign up

Export Citation Format

Share Document