Evaluation of minimum inhibitory concentration and antimicrobial activity of basil extract (Ocimum basilicum L.) incorporated to the mouthwash

AIM: This study has as aim to evaluate the minimum inhibitory concentration and the antimicrobial activity of basil extract incorporated to the mouthwash against the bacteria S.mutans. MATERIAL AND METHODS: For the study, the hydro alcoholic basil extract (Ocimum basilicum L.) incorporated to the mouthwash was used in order to evaluate the effect of this formulation on bacteria and its Minimum Inhibitory Concentration (MIC). As positive control, TBS + S. mutans was used; as negative control, only the TBS; the fluid hydro alcoholic basil extract 20% and the concentrated basil extract 12%, both incorporated to the mouthwash, were also evaluated. As MIC verification method and antimicrobial activity, the micro dilution was used in the concentrations: pure, 1/2, 1/4, 1/8, 1/16, 1/32, 1/64, 1/128; and carried out in triplicate. The microtiter plates were incubated and evaluated after 24 and 72hs. RESULTS: The results showed there was no antimicrobial activity of mouthwash associated to the fluid and concentrated basil extract. However, the mouthwash insulated showed antimicrobial activity only as pure; other dilutions did not presented the same result. CONCLUSION: Before the findings in this study, it is possible conclude that hydro alcoholic basil extract incorporated to the mouthwash did not present antimicrobial activity against the bacteria S. mutans.

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In vitro evaluation of the antimicrobial activity of Basil (Ocimum baslicum L.) and Coriander (Coriandrum satirum L.) oil extracts on Streptococus mutans

Journal of Research in Dentistry ◽

10.19177/jrd.v5e2201740-45 ◽

2018 ◽

Vol 5 (2) ◽

pp. 40 ◽

Cited By ~ 1

Author(s):

Mariana Lourenço ◽

Adilson Cesar Abreu Bernardi ◽

Nadia Lunardi ◽

Rodolfo Jorge Boeck Neto ◽

Patricia Sigilló Mazzoni Bernardi ◽

...

Keyword(s):

Antimicrobial Activity ◽

Minimum Inhibitory Concentration ◽

Plant Extracts ◽

Inhibitory Activity ◽

Inhibitory Concentration ◽

Growth Control ◽

Ocimum Basilicum ◽

Coriandrum Sativum ◽

Microdilution Method

Plant extracts and essential oils show efficiency on growth control in a wide variation of microorganisms, including filamentous fungi, yeasts and bacteria. To evaluate antimicrobial activity of plant extracts, determine the lower quantity of substance to inhibit the microorganism test growth is necessary. This value is known as Minimum Inhibitory Concentration (MIC). This study had as aim to verify the antimicrobial action and the Minimum Inhibitory Concentration (MIC) of basil (Ocimum basilicum L.) and coriander (Coriandrum sativum L.) oil extract before S. mutans (ATCC 25175) strains. Antimicrobial activity determination was carried out by microdilution method and performed according to recommendations of CLSI (previously known as NCCLS), standard M7-A6 (NCCLS, 2003) for bacteria, and standard M27-A2 (NCCLS, 2002). All the experiments were carried out in triplicate. Results showed the Minimum Inhibitory Concentration (MIC) determination by microdilution method in broth showed Ocimum basilicum L. and Coriandrum sativum L. extract oils presented inhibitory activity before S. mutans strain. Basil in 1:4 concentration is bacteriostatic and in 1:3 concentration is bactericide. Coriander in 1:2 concentration is bacteriostatic and in 1:1 concentration is bactericide. We concluded that basil presented higher inhibitory activity regarding to the coriander. We also observed as bigger the extract dilution, lower their effectivity.o assess the attitude and practice of dental professionals towards using of advance Radiographic technique.

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Uji Aktivitas Antifungi Ekstrak Rimpang Kencur (Kaempferia galanga L.) Terhadap Saprolegnia sp. Secara In Vitro [Antifungal Activity Test Of Kaempferia galanga L. Extract To Saprolegnia sp. By In Vitro]

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v5i1.11420 ◽

2019 ◽

Vol 5 (1) ◽

pp. 23

Author(s):

Rahayu Kusdarwati, Ayu Ratnaningtyas, Dewa Ketut Meles

Keyword(s):

Minimum Inhibitory Concentration ◽

Antifungal Activity ◽

Inhibitory Concentration ◽

Positive Control ◽

Negative Control ◽

Fresh Water Fish ◽

Minimum Fungicidal Concentration ◽

Kaempferia Galanga ◽

Activity Test

Abstract Saprolegnia sp. is a fungus that causes the Saprolegniasis disease can infection eggs and fresh water fish. Treatment Saprolegniasis done using chemical drugs, however the use of drugs is bad for the environment and biota. The purpose of the research was to determined the antifungal activity include a minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) from Kaempferia galanga L. to Saprolegnia sp. by in vitro. This research used 9 different concentrations of Kaempferia galanga L extract were 50%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0, 39%, 0.2%, positive control used H2O2 3% and negative control used DMSO 10%. The results showed that the extract of Kaempferia galanga L had an antifungal activity were inhibits and kill with minimum inhibitory concentration (MIC) was 0.39% equivalen with 3,9 mg/ml and minimu fungicidal concentration (MBC) was 1.56% equivalen with 15,6 mg/ml. The existence of antifungal activity against Saprolegnia sp. by in vitro caused by some active compounds from the extracts of the Kaempferia galanga L. are polyphenolic compounds, flavonoin, saponins and essential oils.

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Ekstrak Umbi Rumput Teki (Cyperus Rotundus) Sebagai Antibakteri Terhadap Staphylococcus Epidermidis Dan Propionibacterium Acnes

Biosfer Jurnal Tadris Biologi ◽

10.24042/biosfer.v9i2.3800 ◽

2018 ◽

Vol 9 (2) ◽

pp. 165-175

Author(s):

Siti Nurjanah ◽

Akhmad Rokiban ◽

Erdy Irawan

Keyword(s):

Antimicrobial Activity ◽

Staphylococcus Epidermidis ◽

Inhibitory Concentration ◽

Propionibacterium Acnes ◽

Positive Control ◽

Negative Control ◽

Cyperus Rotundus ◽

Mean Diameter ◽

Inhibition Zones ◽

The Mean

The tuber of cyperus rotundus are used to tradisional medicine. C. rotundus known to contain compounds of secondary metabolites such as alkoloids, sineol, pinene, siperon, rotunal, siperenon, siperol, tannins, flavonoids and scientifically it has been believed to have antimicrobial activity. Empirically this plant are used as an antibacterial, antitumor, anticancer, and antiallergic. The purpose of this study were determining the inhibition of extract of Cyperus rotundus against tubers of Staphylococcus epidermidis and Propionibacterium acnes. In this study C. rotundus extracted by maceration using 70% ethanol. The extracts tested against S. epidermidis and P. acnes using disc blank method. The results obtained from the mean diameter of the inhibition zones of exstract at concentrations of 20%, 40%, 60%, 80%, and 100% on S. Epidermidis 8.03 mm, 11.43 mm, 15.04 mm, 15.83 mm and 19.98 mm respectively and P. acnes 11.59 mm, 13.35 mm, 17.17 mm, 20.44 mm, and 30.08 mm respectively Aquades used as negative control and erythromycin used as positive control. Testing were continued by determining the Minimum Inhibitory Concentration (MIC). The result of MIC for S. epidermidis was 15% and P. acnes was 11%. The analysis showed that the antibacterial activity of the tubers extract is bacteriostatic for S. epdermidis and bacterisid for P. acnes.

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Antimicrobial potential of Casearia sylvestris against oral bacteria

Revista de Odontologia da UNESP ◽

10.1590/1807-2577.11015 ◽

2016 ◽

Vol 45 (4) ◽

pp. 214-218 ◽

Cited By ~ 1

Author(s):

Amanda Henriques CAVALHEIRO ◽

Ana Paula Terossi GODOI ◽

Tatiane Cruz de CARVALHO ◽

Alma Blasida CATIRSE ◽

Niege Araçari Jacometti Cardoso FURTADO

Keyword(s):

Antimicrobial Activity ◽

Minimum Inhibitory Concentration ◽

Essential Oil ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Antimicrobial Activities ◽

Positive Control ◽

Oral Bacteria ◽

Volatile Fraction ◽

Casearia Sylvestris

Abstract Aim The aim of this study was to obtain Casearia sylvestris leave extracts by different extractive methods, including the obtention of essential oil, in order to compare their antimicrobial activities to conventional mouthwash chlorhexidine against oral bacteria. Material and method For this evaluation, extracts from the leaves were obtained by different methods of extraction (infusion, decoction, maceration and percolation) using different solvent systems: water 100%, ethanol 100%, methanol 100%, water: ethanol 3:7; water: ethanol 7:3; water: methanol 7:3 and water: methanol 3:7. The essential oil, which corresponds to a volatile fraction, was obtained by hydrodistillation using Clevenger modified apparatus. The microdilution broth method was used to determine the values of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for the following microorganisms: Streptococcus mutans ATCC 25175, S. mitis ATCC 49456, S. sanguinis ATCC 10556, S. salivarius ATCC 25975, Lactobacillus casei ATCC 11578 and Enterococcus faecalis ATCC4082. Chlorhexidine gluconate was used as a positive control. Result All extracts evaluated in the used protocol displayed MIC values higher than 400 µg/mL and few showed bactericidal activity. The antimicrobial activity of essential oil was higher than the activity of the extracts, and the best minimum inhibitory concentration and minimum bactericidal concentration values were obtained against L. casei (MIC of 0.023 µg/mL and MBC of 0.046 µg/mL) and S. mutans (MIC of 25 µg/mL and MBC of 50 µg/mL), respectively. Conclusion The essential oil of Casearia sylvestris has significant antimicrobial activity against oral microorganisms.

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Synergistic Effects between Mentha Piperita, Mentha Longifolia and Ocimum Basilicum on Different Bacterial Strains

International Journal of Chemistry ◽

10.5539/ijc.v7n2p170 ◽

2015 ◽

Vol 7 (2) ◽

pp. 170 ◽

Cited By ~ 1

Author(s):

Aveen Adham

Keyword(s):

Antimicrobial Activity ◽

Minimum Inhibitory Concentration ◽

Bacterial Strain ◽

Inhibitory Concentration ◽

Synergistic Effects ◽

Ocimum Basilicum ◽

Mentha Piperita ◽

Dilution Method ◽

Bacterial Strains ◽

Mentha Longifolia

The present study aimed to quantitatively evaluate antimicrobial activity of Mentha piperita, Mentha longifolia and Ocimum basilicum from Lamiaceae family, compare between them and to evaluate the type of interaction between them by microbroth dilution method and calculation of fractional inhibitory concentration. Dried leave of Mentha piperita, Mentha longifolia and Ocimum basilicum were extracted using same extraction method and solvent the highest ratio yielded by Mentha longifolia 9% and lowest ratio yielded by Ocimum basilicum 6%. Mentha piperita exhibit the lowest minimum inhibitory concentration value ranged between 1.5-<0.1 mg/ml, followed by Mentha longifolia and Ocimum basilicum ranged between 3-<0.1mg/ml with minimum bactericidal concentration ranged between 6-0.1875. On combination of leave extract Mentha piperita with Mentha longifolia exhibit the lowest minimum inhibitory concentration value ranged between 0.1875-<0.05 mg/ml, while combination of Mentha longifolia with Ocimum basilicum revealed minimum inhibitory concentration ranged between 0.75-<0.05. Among tested bacterial strain Streptococcus mitis showed high sensitivity against all tested leave extract alone and in combination form. The combinations of Mentha piperita, Mentha longifolia and Ocimum basilicum showed synergistic effects against most of bacteria. Mentha longifolia and Ocimum basilicum revealed antagonistic effect against Staphylococcus aureus. As a result three Lamiaceae species showed broad spectrum antimicrobial activity against isolated bacterial strain and their activity in combination form more pronounced than alone.

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PENGARUH EKSTRAK BIJI Ganitri (Elaeocarpus sphaericus Schum.) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli

JURNAL BIOSENSE ◽

10.36526/biosense.v2i01.372 ◽

2020 ◽

Vol 2 (01) ◽

pp. 28-37

Author(s):

Ega Bramaseta ◽

Tristi Indah Dwi Kurnia ◽

Hasyim As'ari

Keyword(s):

Escherichia Coli ◽

Minimum Inhibitory Concentration ◽

Inhibitory Concentration ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Seed Extract ◽

Growth Of Bacteria

This research was conducted in the laboratory of Biology University PGRI Banyuwangi to determine the effect of ganitri seeds extract (Elaeocarpus sphaericus Schum.) in inhibiting the growth of bacteria Escherichia coli and to determine the Minimum Inhibitory Concentration (MIC) of ganitri seeds extract (Elaeocarpus sphaericus Schum.). The method is using wells with 1% tetracycline positive control and negative control of sterile distilled water. Serial concentrations ganitri seed extract (Elaeocarpus sphaericus Schum.) is used by 10%, 20%, 30%, 40%, and 50%, and in the MIC test using serial concentrations of 1%, 2.5%, 5%, 7.5% and 10%. Based on the test results, the largest inhibition zone is shown by a concentration of 50% with an average diameter of 0,954 cm zone of inhibition and inhibition zone indicated smallest concentration of 10% with an average diameter of 0,215 cm inhibition zone. ANOVA test result value (F.Hit> F.Table) with F.Hit amounted to 33,512 and F.Tabel significance value of 2.85 and 0.000 (P <0.05), as the value of P <0.05 then it can be concluded that there is a concentration of ganitri seeds extract (Elaeocarpus sphaericus Schum.) on the growth of test bacteria. The results of Duncan test showed the ganitri seeds extract (Elaeocarpus sphaericus Schum.) at a concentration of 50% have significantly different inhibatory zone by treatment with 10%, 20%, 30%, 40%, positive control, and negative control. While MIC ganitri seed ethanol extract (Elaeocarpus sphaericus Schum.) Were still able to inhibit the growth of test bacteria at a concentration of 1% with an average diameter of 0.037 cm inhibition zone. The Minimum Inhibitory concentration (MIC) ganitri seed extract (Elaeocarpus sphaericus Schum.) shown by a concentration of 1%, where this concentration is still able to inhibit the growth of bacteria Escherichia coli. Keyword: Ganitri seed extract, Bacteria Escherichia coli, Serial concentration, Minimum Inhibitory concentration (MIC), Inhibition zone.

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Green and Simple Synthesis of Silver Nanoparticles by Aqueous Extract of Perovskia abrotanoides: Characterization, Optimization and Antimicrobial Activity

Current Pharmaceutical Biotechnology ◽

10.2174/1389201020666190618121218 ◽

2020 ◽

Vol 21 (11) ◽

pp. 1129-1137 ◽

Cited By ~ 1

Author(s):

Somayeh Mirsadeghi ◽

Masoumeh F. Koudehi ◽

Hamid R. Rajabi ◽

Seied M. Pourmortazavi

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Minimum Inhibitory Concentration ◽

Plant Extract ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Extraction Temperature ◽

Silver Particles ◽

Silver Nps ◽

Perovskia Abrotanoides

Background: Herein, we report the biosynthesis procedure to prepare silver nanoparticles as reduction and capping agents with the aqueous plant extract of Perovskia abrotanoides. Methods: The therapeutic application of silver nanoparticles entirely depends on the size and shape of the nanoparticles therefore, their control during the synthesis procedure is so important. The effects of synthesis factors, for example, silver ion concentration, the mass of plant extract, reaction time and extraction temperature, on the size of silver particles were considered and optimized. Several analytical methods were used for the characterization of silver NPs including FT-IR and UV–Vis spectrophotometer, XRD and SEM. Results: The results showed that the mean size of the silver particles was about 51 nm. Moreover, the antibacterial properties of biosynthesized silver NPs were investigated by the minimum inhibitory concentration, minimum bactericidal concentration, and Well-diffusion tests. The minimum inhibitory concentration/ minimum bactericidal concentration values of silver NPs and aqueous plant extract versus Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and Gram-negative bacteria (E. coli) were 3.03/0.00, 1.20/0.01, 3.06/0.00, 0.98/1.04, 1.00/0.05 and 1.30/0.03 (mg/mL), respectively. Conclusion: The antimicrobial activity study displayed that the synthesized silver nanoparticles by plant extract have better antimicrobial properties compared to aqueous plant extract of Perovskia abrotanoides.

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Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts

Revista de Biología Tropical ◽

10.15517/rbt.v66i4.33140 ◽

2018 ◽

Vol 66 (4) ◽

pp. 1519

Author(s):

Mailen Ortega Cuadros ◽

Adriana Patricia Tofiño Rivera ◽

Luciano Jose Merini ◽

Maria Cecilia Martinez Pabon

Keyword(s):

Antimicrobial Activity ◽

Dental Caries ◽

Essential Oil ◽

Streptococcus Mutans ◽

Human Fibroblasts ◽

Positive Control ◽

Negative Control ◽

Cymbopogon Citratus ◽

Significant Difference ◽

Efficient Alternative

Dental caries is a pathology of multifactorial origin and currently natural products are an efficient alternative treatment; The work sought to evaluate the antimicrobial activity of the Cymbopogon citratus essential oil and the citral and myrcene components against Streptococcus mutans ATCC UA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. The viability effect against Streptococcus mutans on biofilms was evaluated through exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1, 0.1, and 0.01 µg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated on keratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control (negative control) and ethanol 1% as vehicle control (positive control). The three substances evaluated had effects on the viability of Streptococcus mutans with mortality between 74% and 96%, without significant difference among them (p > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24-h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studied further as potential co-adjuvants to prevent dental caries that cause minor adverse effects

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Antimicrobial Activity of Bauhinia Purpurea (L) by Minimum Inhibitory Concentration (MIC) Method

IOSR Journal of Pharmacy and Biological Sciences ◽

10.9790/3008-1201026971 ◽

2017 ◽

Vol 12 (01) ◽

pp. 69-71

Author(s):

Shamala T ◽

A.M. Krupanidhi ◽

Shanmukhappa S ◽

B.E. Basavarajappa ◽

Jayamma kulkarni

Keyword(s):

Antimicrobial Activity ◽

Minimum Inhibitory Concentration ◽

Inhibitory Concentration ◽

Bauhinia Purpurea

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Study and evaluation of antimicrobial activity and antioxidant capacity of dry extract and fractions of leaves of Raphanus sativus var. oleiferus Metzg.

Bioscience Journal ◽

10.14393/bj-v36n2a2020-41848 ◽

2020 ◽

Vol 36 (2) ◽

Cited By ~ 1

Author(s):

Ana Flávia da Silva ◽

Marisa de Oliveira Lopes ◽

Cláudio Daniel Cerdeira ◽

Ingridy Simone Ribeiro ◽

Isael Aparecido Rosa ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Minimum Inhibitory Concentration ◽

Ethyl Acetate ◽

Raphanus Sativus ◽

Inhibitory Concentration ◽

Ethyl Acetate Fraction ◽

Antimicrobial Activities ◽

Dry Extract ◽

Antioxidant And Antimicrobial Activities

The radish (Raphanus sativus L.) is a vegetable of the Brassicaceae family cultivated worldwide and has several medicinal properties. Its biological activities are related to various secondary metabolites present in the species, especially phenolics. Thus, the objectives of this study were the chemical analysis and evaluation of the antioxidant and antimicrobial activities of the dry extract and fractions of the fodder turnip leaves (R. sativus var. oleiferus Metzg.). Samples were analyzed by mass spectrometry and the antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical method and the reducing power method. Antimicrobial activity was determined by the agar diffusion and microdilution methods. The total phenols were concentrated in the butanol fraction (121.27 mg GAE/g) and the flavonoids were concentrated in the ethyl acetate fraction (98.02 mg EQ/g). The ethyl acetate fraction showed the best antioxidants results, with 83.45% of free radical scavenging and 11.34% of ferric ions reduction. The analysis of antimicrobial activity showed that the dry extract had the highest average zone of inhibition against Bacillus subtilis (18.67 mm). Smaller values of the minimum inhibitory concentration for Micrococcus luteus were, and the ethyl acetate fraction showed a lower minimum inhibitory concentration (0.1 mg/ml) for that microorganism. There was a strong correlation between the antioxidant activity and the content of phenols and flavonoids. The results showed the potential antioxidant and antimicrobial activities of this extract with the ethyl acetate fraction being most promising for further studies.

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